Application Guide - QIAxcel Advanced

98 QIAxcel Advanced Application Guide 10/2016 Detection of genetically modified plants using the QIAxcel® System BeeNa Lee, QIAGEN, Irvine, CA The QIAxcel System was successfully used to detect DNA derived from genetically modified organisms (GMOs) at a level suitable for GMO testing according to EU standards. Introduction According to current EU regulations that went into effect in 2004, food and feed that contain at least 0.9% content from GMOs, or that were derived from GMOs, must be labeled as such. Each GMO that is present must be specifically declared using the system of unique identifiers published by the EU. For products for which compliance can not be conclusively demonstrated by analysis (such as for highly refined materials), the non- GMO origin of the material must be documented. Enforcement of the regulation depends upon the availability of accurate methods for detection and tracing of GMO materials. This report describes the validation of a reliable and cost-effective system for GMO detection and analysis which facilitates compliance with EU guidelines. The following performance characteristics were determined for GMO material mixed with non-modified plant material: • DNA concentration • Fragmentation state of DNA • Specificity The QIAxcel System. The fully automated size separation and quantification capability of the QIAxcel System provides unmatched resolution, speed, and throughput.

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