Application Guide - QIAxcel Advanced

100 QIAxcel Advanced Application Guide 10/2016 GMO (%) 0 4.0 3.5 3.0 2.5 2.0 1.5 1.0 0.5 0 0 2 0 1 Labeling required Labeling not required Normalized peak area Reference Liu M.S. and Amirkhanian V.D. (2003) DNA fragment analysis by an affordable multiple-channel capillary electrophoresis system. Electrophoresis 24, 93.0. Conclusions • This system detected as few as 125 copies of GMO- origin target mixed with 50 ng genomic DNA from non- modified plants, corresponding to 0.25% GMO content (see Table 1). This result indicates that the sensitivity of the system allows detection of GMO content well below the cutoff limit for labeling (0.9%). • The QIAxcel System includes a consumable multi-channel cartridge that can be used to inject and analyze multiple DNA samples simultaneously: up to 12 samples can be analyzed in less than 7 minutes and 96 samples in a multiwell plate in less than 50 minutes. This throughput is sufficient to meet the needs of many GMO-testing facilities. Figure 2. LOD of the QIAxcel System. The LOD was calculated for the quantitative normalized area (peak area) of the amplified 35S product at a defined number of copies per reaction. According to the EU GMO regulation, the limit of detection for a GMO marker must be below 0.9%. Food containing over 0.9% GMO content must be labeled. QIAxcel Kits. Precast, reusable gel cartridges allow up to 200 runs of 12 samples to be performed. Template copies GMO content (%) Detected 2000 4 Yes 1000 2 Yes 500 1 Yes 250 0.5 Yes 125 0.25 Yes 62 0.125 No 31 0.06 No No template control 0.0 No Table 1. Detection of GMO content 20004 Yes 10002 Yes 5001 Yes 2500.5 Yes 1250.25 Yes 620.125 No 310.06 No

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