Application Guide - QIAxcel Advanced

QIAxcel Advanced Application Guide 10/2016 123 Table 1. Fragment sizes after digestion of 359-bp mitochondrial cytb fragment with restriction enzymes: Data are presented for nine animal species. Fragment sizes are given in base pairs. Restriction enzyme Animal species HaeIII AluI HinfI RsaI Cattle 74, 285 190, 169 44, 198, 117 359 Pig 74, 132, 153 115, 244 359 359 Sheep 74, 126, 159 359 161, 198 359 Deer 74, 285, 126, 159 359 44, 315 359 Rabbit 44, 132, 30 359 233, 126, 153 359 Chicken 74, 21, 159 359 105, 161, 188, 10 149, 210 Duck 55, 286, 18 359 161, 198 359 Turkey 74, 285 359 161, 198 149, 109, 101 Goose 74, 126, 159 130, 229 359 154, 205 A1 A5 A6 A7 A8 A9 A10 C2 C3 C4 C5 C6 C7 D3 D4 D5 D6 D7 D8 E3 E4 E5 E6 E7 E8 – 600 – 500 – 400 – 300 – 250 – 200 – 75 – 50 – 25 – 15 – 150 – 100 [bp] Figure 2. QIAxcel analysis of six commercial food samples. The samples were as follows — FF1: pork pâté (100% pig); FF2: hamburger meat (100% beef); FF3: Parmentier beef hash (100% beef); FF4: pork terrine (100% pig); FF5: pork liver mousse (100% pig); FF6: poultry liver terrine of (75% pig and 25% poultry). These samples were digested by the four studied enzymes, as follows — Lanes A5–A10: AluI; Lanes C2–C7: HinfI; Lanes D3–D8: HaeIII; Lanes E3–E8: Rsal. Lane A1: DNA Size Marker 25 – 500 bp. Table 2. Summary of the analysis of the six samples, based on the data from Figure 2. Sample Expected result Observed result Compliance FF1 (Pork pâté) Pig Pig Yes FF2 (Hamburger meat) Beef (cattle) Beef + Pig No FF3 (Parmentier beef hash) Beef Beef + Pig (traces) No FF4 (Pork terrine) Pig Pig Yes FF5 (Pork liver mousse) Pig Pig Yes FF6 (Poultry liver terrine) Pig + Poultry Pig + Chicken Yes Cattle 74, 285190, 16944, 198, 117359 Pig 74, 132, 153115, 244359359 Sheep 74, 126, 159359161, 198359 Deer 74, 285, 126, 15935944, 315359 Rabbit 44, 132, 30359233, 126, 153359 Chicken 74, 21, 159359105, 161, 188, 10149, 210 Duck 55, 286, 18359161, 198359 Turkey 74, 285359161, 198149, 109, 101 Goose 74, 126, 159130, 229359154, 205

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