Application Guide - QIAxcel Advanced

QIAxcel Advanced Application Guide 10/2016 75 was used to estimate the size of the PCR amplicons. Sizing was done automatically using the QIAxcel ScreenGel® software. Allele calling was performed automatically using a peak calling table that defines the size of the alleles according to theoretical tables (2) and a tolerance in % for each locus to exclude any overlapping data. Then the results were merged into an Excel® spreadsheet and submitted to the free online genotyping database. Reference sizing method Sanger sequencing was used as a reference method to determine the amplicon sizes. Table 1. Summary of MIRU-VNTR analyzed in this study Alias/Locus Minimum size (bp) Maximum size (bp) Allele Distribution 0 1 2 3–3s 4 5 6 7 8 9 10 15 Mixture 15-locus MIRU-VNTR panel (n = 82) MIRU 04/580 250 757 2 63 3 7 2 3 2 MIRU 10/960 590 1262 8 48 17 2 3 2 1 1 MIRU 16/1644 565 821 1 4 19 57 1 MIRU 26/2996 335 649 4 8 10 6 40 2 11 1 MIRU 31/3192 492 816 1 10 24 3 40 3 1 MIRU 40/802 398 692 8 9 42 10 12 1 Mtub 04/424 602 920 1 25 12 38 6 ETR C/577 270 445 3 14 63 2 Mtub 21/1955 164 1053 1 16 14 8 42 1 QUB-11b/2163b 140 626 6 12 6 11 2 38 5 1 1 ETR A/2165 274 732 1 6 17 50 2 5 1 Mtub 30/2401 248 533 1 7 22 52 Mtub 39/3690 387 783 7 53 8 9 2 1 1 1 QUB-26/4052 385 1317 1 6 4 7 17 11 29 4 1 2 QUB-4156/4156 560 849 4 8 56 8 6 24-locus MIRU-VNTR (n = 66) MIRU 02/154 456 513 3 62 1 MIRU 20/2059 508 605 2 64 MIRU 23/2531 306 472 1 59 5 1 MIRU 24/2687 439 505 59 7 MIRU 27/3007 644 665 65 1 MIRU 39/4348 584 700 1 17 47 1 Mtub 29/2347 451 579 1 7 58 ETR B/2461 403 647 1 57 1 6 1 Mtub 35/3171 386 492 1 64 1 0123–3s 4567891015 Mixture MIRU 04/58025075726337232 MIRU 10/96059012628481723211 MIRU 16/16445658211419571 MIRU 26/299633564948106402111 MIRU 31/31924928161102434031 MIRU 40/802398692894210121 Mtub 04/42460292012512386 ETR C/577270445314632 Mtub 21/19551641053116148421 QUB-11b/2163b 140626612611238511 ETR A/2165274732161750251 Mtub 30/2401248533172252 Mtub 39/3690387783753892111 QUB-26/405238513171647171129412 QUB-4156/4156560849485686 MIRU 02/1544565133621 MIRU 20/2059508605264 MIRU 23/253130647215951 MIRU 24/2687439505597 MIRU 27/3007644665651 MIRU 39/4348584700117471 Mtub 29/23474515791758 ETR B/2461403647157161 Mtub 35/31713864921641

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