Application Guide - QIAxcel Advanced

QIAxcel Advanced Application Guide 10/2016 77 For up-to-date licensing information and product-specific disclaimers, see the respective QIAGEN kit handbook or user manual. QIAGEN kit handbooks and user manuals are available at www.qiagen.com or can be requested from QIAGEN Technical Services or your local distributor. See the benefits of how automated gel electrophoresis at www.qiagen.com/QIAxcel! Trademarks: QIAGEN® , Sample to Insight® , QIAxcel® , ScreenGel® , HotStarTaq® (QIAGEN Group); NuSieve® (Lonza Group); Excel® (Microsoft Corporation). Registered names, trademarks, etc. used in this document, even when not specifically marked as such, are not to be considered unprotected by law. Ordering www.qiagen.com/shop Technical Support support.qiagen.com Website www.qiagen.com Conclusions • The precision and accuracy of sample size estimation of Mycobacterium tuberculosis DNA with the QIAxcel Advanced method is compatible with that of conventional methods, and the repeatability and reproducibility are better. • The QIAxcel Advanced method is significantly faster and less expensive. • Implementing QIAxcel Advanced-based MIRU-VNTR genotyping of M. tuberculosis in the field would benefit epidemiological studies on tuberculosis worldwide. References 1. Supply, P. et al. (2006) Proposal for Standardization of Optimized MIRU-VNTR Typing of Mycobacterium Tuberculosis. J. Clin. Micr. 44(12) 4498. 2. http://www.miru-vntrplus.org/MIRU/files/MIRU-VNTRtypingmanualv6.pdf 3. Gauthier, M. et al. (2014) High-Throughput MIRU-VNTR Genotyping for Mycobacterium Tuberculosis Epidemiological Studies. J. Clin Micr. Accepted for publication November 2014. Ordering Information Product Contents Cat. no. QIAxcel Advanced System Capillary electrophoresis device: includes computer, QIAxcel ScreenGel software, and 1-year warranty on parts and labor 9001941 QIAxcel DNA High Resolution Kit (1200) QIAxcel DNA High Resolution Cartridge, Buffers, Mineral Oil, QX Intensity Calibration Marker, 12-Tube Strips 929002 QIAxcel Alignment Marker 15 bp/3 kb (1.5 ml) Alignment marker with 15 bp and 3 kb fragments 929522 QIAxcel DNA Size Marker 100 bp – 2.5 bp (50 µl) DNA size marker with fragments of varying size from 100 to 2500 bp; concentration 100 ng/µl 929559

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