Application Guide - QIAxcel Advanced

80 QIAxcel Advanced Application Guide 10/2016 Conclusions • ERIC PCR fragments were separated and unambiguously identified using the QIAxcel system and BioCalculator Software. Subsequent comparison of the ERIC PCR patterns led to the precise determination of the genetic relationship of bacterial strains. • The QIAxcel DNA High Resolution Kit resolves ERIC PCR fragments more effectively than agarose or polyacrylamide gels, providing greater sizing accuracy and improved sensitivity. The results are fully reproducible due to controlled running conditions and automated data acquisition. Since up to 96 samples can be analyzed in a single run, the QIAxcel system yields more information from DNA fingerprints while saving time. • Since the QIAxcel capillary electrophoresis uses only minute quantities of DNA through electrokinetic injection, the samples are retained for downstream procedures, such as sequencing or cloning. References 1. Bradić, M., Sikora, S., Redžepović, S., and Štafa, Z. (2003) Genetic identification and symbiotic efficiency of an indigenous Sinorhizobium meliloti field population. Food Technol. Biotechnol. 41, 69. 2. Hartmann, A., Giraud, J.J., and Catroux, G. (1998) Genotypic diversity of Sinorhizobium (formerly Rhizobium) meliloti strains isolated directly from a soil and from nodules of alfalfa (Medicago sativa) grown in the same soil. FEMS Microbiol. Ecol. 25, 107. 3. Sikora, S., Redžepović, S., Pejič, I., and Kozumplik, V. (1997) Genetic diversity of Bradyrhizobium japonicum field population revealed by RAPB fingerprinting. J. Appl. Microbiol. 82, 527. 4. Huić, B., et al. (2008) Influence of different Sinorhizobium meliloti inocula on abundance of genes involved in nitrogen transformations in the rhizosphere of alfalfa (Medicago sativa L.) Environmental Microbiol. 10, 2922. 5. Versalovic, J., Koeuth, T., and Lupski, J.R. (1991) Distribution of repetitive DNA sequences in eubacteria and application to fingerprinting of bacterial genomes. Nucleic Acids Res. 19, 6823. 6. De Brujin, F.J. (1992) Use of repetitive (repetitive extragenic palindromic and enterobacterial repetitive intergeneric consensus) sequences and the polymerase chain reaction to fingerprint the genomes of Rhizobium meliloti isolates and other soil bacteria. Appl. Environ. Microbiol. 58, 2180.

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