Application Guide - QIAxcel Advanced

QIAxcel Advanced Application Guide 10/2016 29 For up-to-date licensing information and product-specific disclaimers, see the respective QIAGEN kit handbook or user manual. QIAGEN kit handbooks and user manuals are available at www.qiagen.com or can be requested from QIAGEN Technical Services or your local distributor. Visit www.qiagen.com/linkage-analysis and find out how automated gel electrophoresis can benefit your lab! Trademarks: QIAGEN® , QIAxcel® , Sample to Insight® (QIAGEN Group). Registered names, trademarks, etc. used in this document, even when not specifically marked as such, are not to be considered unprotected by law. Ordering www.qiagen.com/shop Technical Support support.qiagen.com Website www.qiagen.com Conclusions These results demonstrated that the QIAxcel system can resolve the size difference of DNA fragments down to just a few base pairs, a resolution that was not achievable using conventional agarose gel electrophoresis. The QIAxcel system provides significant advantages in determination of genotype for gene linkage studies. M 1 2 3 4 5 6 7 8 9 220 bp – 298 bp – 1 2 3 4 5 6 7 8 9 – 242 bp 298 bp*– 233 bp – 40 bp*– Figure 1. Results using 3% agarose gel. M: Marker; Lane 1: TL line (P0 ); Lane 2: India line (P0 ); Lane 3: Individual (F1 ) obtained from TL x India cross; Lanes 4, 5, 6: Normal individual (F2 ) of brood; Lanes 7, 8, 9: Homozygous mutant (F2 ) of brood. Figure 2. Results using the QIAxcel system with the QIAxcel DNA High Resolution Kit. Lane 1: TL line (P0 ); Lane 2: India line (P0 ); Lane 3: Individual (F1 ) obtained from TL x India cross; Lanes 4, 5, 6: Normal individual (F2 ) of brood; Lanes 7, 8, 9: Homozygous mutant (F2 ) of brood. * Alignment marker. Ordering Information Product Contents Cat. no. QIAxcel Advanced system Capillary electrophoresis device, including computer, and ScreenGel Software; 1-year warranty on parts and labor 9001941 QIAxcel DNA High Resolution Kit (1200) QIAxcel DNA High Resolution Gel Cartridge, Buffers, Mineral Oil, QX Intensity Calibration Marker, 12-Tube Strips 929002 M 123456789 123456789

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