Application Guide - QIAxcel Advanced

QIAxcel Advanced Application Guide 10/2016 117 Results and Discussion The amplicons from the singleplex and multiplex PCR assays were analyzed using the QIAxcel system and QIAxcel DNA High Resolution Kit (Figure 1). An overview of the assays and the fragments detected is presented in Table 1. All of the nuts present on the ingredients list of the samples were accurately identified. An electropherogram of sample 16 is also presented to illustrate the successful application of the newly developed PCR method (Figure 2). The limit of detection for all of the nut species based on singleplex assays using 100% nut material was less than 20 pg of nut DNA. 75 – – 25 – 50 – 75 – 100 – 150 – 200 – 250 – 300 – 400 99 – 110 – 134 – bp 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 bp 15 Figure 1. Successful use of the QIAxcel system for DNA fragment analysis. DNA fragments from macadamia, peanut, hazelnut, and almond are clearly visible. Lane 1: Macadamia (75 bp); lanes 3, 4, 11, 13, 14, 16, and 17: peanut (99 bp); lanes 6, 8, 10,  15 and 16: hazelnut (110 bp); lanes 2, 5, 7, 9, 12, 15, 16, and 17: almond (134 bp); lane 18: QX DNA Size Marker 25–500 bp. Figure 2. Electropherogram displaying the result from the multiplex PCR, sample 16 (cereal bar). DNA fragments from peanut (99 bp), hazelnut (110 bp), and almond (134 bp) were detected. The ingredients listed in the cereal bar included almonds (15%), peanuts (15%), and hazelnuts (9%). bp 123456789101112131415161718 bp

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