Application Guide - QIAxcel Advanced

36 QIAxcel Advanced Application Guide 10/2016 Conclusions • The fragments amplified from the Cre transgene and from the myogenin control (320 bp and 250 bp, respectively) were easily distinguished using the QIAxcel system, allowing reliable identification of transgenic mice. The sizing accuracy and sensitivity obtained using the QIAxcel system is superior to conventional methods, such as agarose gel electrophoresis (data not shown). • Due to automated electrophoresis analysis as well as automated data acquisition, the QIAxcel system ensures safe and reliable results. • Because the QIAxcel system enables short running times and analysis of up to 96 samples in a single run, it is ideally suited for medium- to high-throughput mouse genotyping, significantly saving time. • QIAxcel analysis results are fully reproducible due to controlled running conditions and automated data acquisition. • Since QIAxcel capillary electrophoresis uses only minute quantities of DNA for electrokinetic injection, the samples are retained for downstream procedures, such as sequencing. References 1. Araki, K., Imaizumi, T., Okuyama, K., Oike, Y., and Yamamura, K. (1997) Efficiency of recombination by Cre transient expression in embryonic stem cells: comparison of various promoters. J. Biochem. 122, 977. 2. Nagy, A. (2000) Cre recombinase: the universal reagent for genome tailoring. Genesis 26, 99. 3. Dali-Youcef, N. et al. (2007) Adipose tissue-specific inactivation of the retinoblastoma protein protects against diabesity because of increased energy expenditure. Proc. Natl. Acad. Sci. 104, 10703. 4. Mataki, C. et al. (2007) Compromised intestinal lipid absorption in mice with a liver-specific deficiency of liver receptor homolog 1. Mol. Cell. Biol. 27, 8330.

Pages Overview