Application Guide - QIAxcel Advanced

58 QIAxcel Advanced Application Guide 10/2016 Conclusions • The QIAxcel capillary electrophoresis system is an optimal tool to resolve amplification products from multiplex PCR samples. Since even very small differences in fragment size could be resolved, it was possible to unambiguously detect immunoglobulin gene rearrangements. This allowed us to determine whether a lymphoid tissue was of monoclonal, oligoclonal, or polyclonal origin. • Fast and accurate detection of gene rearrangement in B-cells facilitates the understanding of the development of malignancies during maturation of B-cells. • The QIAxcel system processes samples in batches of 12 and allows analysis of up to 96 samples without manual intervention. Results can be displayed as a gel-like image as well as an electropherogram. The size and concentration data can be exported as a Microsoft® Excel® table. • QIAxcel capillary electrophoresis uses electrokinetic injection of minute quantities of DNA, allowing the samples to be retained for downstream procedures, such as sequencing. References 1. Parham, P. (2009) The Immune System. 3rd ed. New York: Garland Science. 2. Jaffe, E.S. et al. eds (2001) World Health Organization Classification of Tumours. Pathology and Genetics of Tumours of Haematopoietic and Lymphoid Tissues. Lyon: IARC Press. 3. van Dongen, J.J.M. et al. (2003) Design and standardization of PCR primers and protocols for detection of clonal immunoglobulin and T-cell receptor gene recombinations in suspect lymphoproliferations: report of the BIOMED-2 Concerted Action BMH4-CT98-3936. Leukemia 17, 2257. Gene S1 S2 S3 S4 S5a S5b IGH-FR3 + – + * + + † + † IGH-FR2 + – + + + † + † IGK-A + – + * + + † + † IGK-B + – + * – – – Table 1. Observed IGH and IGK gene rearrangements Monoclonal gene rearrangement present (+) or absent (–). * Oligoclonality. † The same monoclonal gene rearrangements were observed in both S5 samples.

Pages Overview