Application Guide - QIAxcel Advanced

QIAxcel Advanced Application Guide 10/2016 49 Figure 4. Ratio chart interpretations of the data from APC samples by the Coffalyser software. A APC control data indicating a ratio of 1, meaning no deletion/ duplication at any of the specific genomic regions. B Heterozygous deletion of specific APC alleles indicated by a ratio tending towards 0.5 (ratio of 0 would indicate homozygous deletion). C Duplication of specific APC alleles indicated by a ratio tending towards 1.5. Mean ± 95% confidence interval of ratio of signal intensity between sample and reference. Green/ blue boxes indicate the 95% confidence range of reference. APC control 01-045.571067 01-045.572632 01-045.578503 05-112.071072 05-112.071434 05-112.101127 05-112.101435 05-112.101975 05-112.118477 05-112.129936 05-112.130843 05-112.139223 05-112.144431 05-112.156040 05-112.164867 05-112.179090 05-112.182586 05-112.185611 05-112.186901 05-112.190704 05-112.191530 05-112.192470 05-112.198522 05-112.201344 05-112.202335 05-112.203069 05-112.204006 05-112.207595 01-065.848260 02-113.606702 04-187.390319 05-132.037610 07-091.680412 09-071.040783 09-134.767853 12-006.796730 14-034.941573 19-061.386348 MUTYH-9-136nt MUTYH-4-346nt MUTYH-1-253nt APC-1-260nt APC-1-274nt APC-2-142nt APC-2-150nt APC-3-171nt APC-4-178nt APC-5-193nt APC-6-202nt APC-7-220nt APC-8-229nt APC-9-246nt APC-10-267nt APC-11-282nt APC-12-289nt APC-13-306nt APC-intr.13-409nt APC-14-312nt APC-15-328nt APC-16-337nt APC-17-365nt APC-18-356nt APC-18-(WT)-418nt APC-18-(WT)-427nt APC-18-382nt APC-18-391nt Reference*-400nt Reference*-238nt Reference*-186nt Reference*-130nt Reference*-297nt Reference*-211nt Reference*-321nt Reference*-373nt Reference*-157nt Reference*-445nt Ratio A B 3 2.5 2 1.5 1 0.5 0 APC duplication 01-045.571067 01-045.572632 01-045.578503 05-112.071072 05-112.071434 05-112.101127 05-112.101435 05-112.101975 05-112.118477 05-112.129936 05-112.130843 05-112.139223 05-112.144431 05-112.156040 05-112.164867 05-112.179090 05-112.182586 05-112.185611 05-112.186901 05-112.190704 05-112.191530 05-112.192470 05-112.198522 05-112.201344 05-112.202335 05-112.203069 05-112.204006 05-112.207595 01-065.848260 02-113.606702 04-187.390319 05-132.037610 07-091.680412 09-071.040783 09-134.767853 12-006.796730 14-034.941573 19-061.386348 MUTYH-9-136nt MUTYH-4-346nt MUTYH-1-253nt APC-1-260nt APC-1-274nt APC-2-142nt APC-2-150nt APC-3-171nt APC-4-178nt APC-5-193nt APC-6-202nt APC-7-220nt APC-8-229nt APC-9-246nt APC-10-267nt APC-11-282nt APC-12-289nt APC-13-306nt APC-intr.13-409nt APC-14-312nt APC-15-328nt APC-16-337nt APC-17-365nt APC-18-356nt APC-18-(WT)-418nt APC-18-(WT)-427nt APC-18-382nt APC-18-391nt Reference*-400nt Reference*-238nt Reference*-186nt Reference*-130nt Reference*-297nt Reference*-211nt Reference*-321nt Reference*-373nt Reference*-157nt Reference*-445nt Ratio C 3 2.5 2 1.5 1 0.5 0 APC deletion 01-045.571067 01-045.572632 01-045.578503 05-112.071072 05-112.071434 05-112.101127 05-112.101435 05-112.101975 05-112.118477 05-112.129936 05-112.130843 05-112.139223 05-112.144431 05-112.156040 05-112.164867 05-112.179090 05-112.182586 05-112.185611 05-112.186901 05-112.190704 05-112.191530 05-112.192470 05-112.198522 05-112.201344 05-112.202335 05-112.203069 05-112.204006 05-112.207595 01-065.848260 02-113.606702 04-187.390319 05-132.037610 07-091.680412 09-071.040783 09-134.767853 12-006.796730 14-034.941573 19-061.386348 MUTYH-9-136nt MUTYH-4-346nt MUTYH-1-253nt APC-1-260nt APC-1-274nt APC-2-142nt APC-2-150nt APC-3-171nt APC-4-178nt APC-5-193nt APC-6-202nt APC-7-220nt APC-8-229nt APC-9-246nt APC-10-267nt APC-11-282nt APC-12-289nt APC-13-306nt APC-intr.13-409nt APC-14-312nt APC-15-328nt APC-16-337nt APC-17-365nt APC-18-356nt APC-18-(WT)-418nt APC-18-(WT)-427nt APC-18-382nt APC-18-391nt Reference*-400nt Reference*-238nt Reference*-186nt Reference*-130nt Reference*-297nt Reference*-211nt Reference*-321nt Reference*-373nt Reference*-157nt Reference*-445nt Ratio 3 2.5 2 1.5 1 0.5 0 B A

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