Application Guide - QIAxcel Advanced

104 QIAxcel Advanced Application Guide 10/2016 The band at 204 bp was also present in lane 2 for S. scombrus, and in lane 4 for Salmo salar. The band at 273 bp appeared in lane 4 for S. salar, and finally, the band at 466 bp was visible in lane 3 for Merluccius merluccius. Table 3 compares sizes of the observed HinfI restriction fragments with the theoretically expected fragments. The deviation average between theoretical and observed data was 4 bp. Experiments with the other enzymes generated Scientific species names Anguilla anguilla Merluccius merluccius Scomber scombrus Boops boops Microstomus kitt Scophthalmus rhombus Coryphaenoides rupestris Mulus surmuletus Sparus aurata Cynoglossus senegalensis Oncorhynchus gorbuscha Stizostedion luciperca; Sander lucioperca Dicentrarchus labrax Oncorhynchus keta Theragra chalcogramma Epinephelus sp. Oncorhynchus kisutch Thunnus albacares Gadus morhua Oncorhynchus mykiss or Salmo gaidneri Thunnus thynnus Glyptocephalus cynoglossus Perca fluviatilis Trachurus trachurus Lates niloticus Pleuronectes platessa Xiphias gladius Lophius sp. Pollachius virens Zeus faber Melanogrammus aeglefinus Salmo salar Merlanguis merlanguis Sardina pilchardus Table 2. List of fish species that could be identified. 1 2 AluI 3 4 [bp] – 600 – 500 – 400 – 300 – 200 – 250 – 100 – 150 – 15 – 50 1 2 HaeIII 3 4 [bp] – 500 – 400 – 300 – 200 – 250 – 100 – 150 – 15 – 50 1 2 HinfI 3 4 [bp] – 400 – 300 – 200 – 250 – 100 – 150 – 15 – 50 Ddel 1 2 AluI 3 4 [bp] – 600 – 500 – 400 – 300 – 200 – 250 – 100 – 150 – 15 – 50 1 2 HaeIII 3 4 [bp] – 500 – 400 – 300 – 200 – 250 – 100 – 150 – 15 – 50 1 2 HinfI 3 4 [bp] – 400 – 300 – 200 – 250 – 100 – 150 – 15 – 50 1 2 Ddel 3 4 [bp] – 400 – 300 – 200 – 250 – 100 – 150 – 15 – 50 TaqI 1 2 AluI 3 4 [bp] – 600 – 500 – 400 – 300 – 200 – 250 – 100 – 150 – 15 – 50 1 2 HaeIII 3 4 [bp] – 500 – 400 – 300 – 200 – 250 – 100 – 150 – 15 – 50 1 2 HinfI 3 4 [bp] – 400 – 300 – 200 – 250 – 100 – 150 – 15 – 50 1 2 Ddel 3 4 [bp] – 400 – 300 – 200 – 250 – 100 – 150 – 15 – 50 1 2 TaqI 3 4 [bp] – 400 – 300 – 200 – 250 – 100 – 150 – 15 – 50 1 2 AluI 3 4 [bp] – 600 – 500 – 400 – 300 – 200 – 250 – 100 – 150 – 15 – 50 1 2 HaeIII 3 4 [bp] – 500 – 400 – 300 – 200 – 250 – 100 – 150 – 15 – 50 1 2 HinfI 3 4 [bp] – 400 – 300 – 200 – 250 – 100 – 150 – 15 – 50 1 2 Ddel 3 4 [bp] – 400 – 300 – 200 – 250 – 100 – 150 – 15 – 50 1 2 TaqI 3 4 [bp] – 400 – 300 – 200 – 250 – 100 – 150 – 15 – 50 1 2 HaeIII 3 4 [bp] – 500 – 400 – 300 – 200 – 250 – 100 – 150 – 15 – 50 1 2 HinfI 3 4 [bp] – 400 – 300 – 200 – 250 – 100 – 150 – 15 – 50 1 2 Ddel 3 4 [bp] – 400 – 300 – 200 – 250 – 100 – 150 – 15 – 50 1 2 TaqI 3 4 [bp] – 400 – 300 – 200 – 250 – 100 – 150 – 15 – 50 Figure 1. A panel of 5 enzymes used to discriminate between different fish species based on fragment analysis using QIAxcel Advanced. Lane 1: laboratory mixture of fresh S. salar, S. scombrus and M. merluccius in the proportions 20:40:40. Lane 2: S. scombrus; Lane 3: M. merluccius. Lane 4: S. salar. 12 34 [bp] 12 34 [bp] 12 34 [bp] 12 34 [bp] 12 34 [bp] 12 34 [bp] 12 34 [bp] 12 34 [bp] 12 34 [bp] 12 34 [bp] 12 34 [bp] 12 34 [bp] 12 34 [bp] 12 34 [bp] 12 34 [bp] 12 34 [bp] 12 34 [bp] 12 34 [bp] 12 34 [bp] 12 34 [bp] 12 34 [bp]

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