Application Guide - QIAxcel Advanced

128 QIAxcel Advanced Application Guide 10/2016 Figure 2. Analysis of Wx-A1 gene types in a segregating F3 generation of wheat. W: wild type; N: mutant; H: heterozygote. Figure 1. Wx gene analysis in a segregating F3 generation of wheat. Genomic DNA was isolated from wild type (CS), Mochi- Otome (MO), and heterozygous (H) plants and amplified using primers for the A. Wx-A1, B. Wx-B1, and C. Wx-D1 genes. PCR products were analyzed on the QIAxcel system using the QIAxcel DNA Screening Kit. The sizes of expected fragments are listed in Table 1. Electropherogram views of fragments amplified from D. heterozygous and E. wild type plants using BDFL and BDR primers. A B C D E H H H H H H H W W W W W W W W W W 389 – W W W N N N H – 370 bp bp CS BDFL and BRD MO H bp – 425 CS BDFL and DRSL MO H bp – 1713 – 2307 Wx-B1 Wx-A1 Wx-D1 Wx-B1 Wx-A1 Wx-D1 H CS CS AFC and AR2 MO H bp – 370 389 – Conclusions • The high-throughput capacity of the QIAxcel system provides an efficient way to handle large numbers of plants from breeding programs and is highly suitable for the wheat breeding industry. • Selection of wild type, mutant, and heterozygous plants is feasible and simple using the QIAxcel system. References 1. Nakamura, T., Vrinten, P., Saito, M., and Konda, M. (2002) Rapid classification of partial waxy wheats using PCR-based markers. Genome 45, 1150.

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