Application Guide - QIAxcel Advanced

22 QIAxcel Advanced Application Guide 10/2016 References 1. Konieczny, A., and Ausubel, F.M. (1993) A procedure for mapping Arabidopsis mutations using co-dominant ecotypespecific PCR-based markers. Plant J. 4, 403. 2. De Carbonnel, M. et al. (2010) The arabidopsis PHYTOCHROME KINASE SUBSTRATE2 protein is a phototropin signaling element that regulates leaf flattening and leaf positioning. Plant Physiol. 152, 1391. Conclusions • The sharp banding patterns achieved with the QIAxcel capillary electrophoresis system simplified and accelerated the routine sizing of wild type and mutant DNA fragments. Due to the accurate sizing of DNA fragments compared to conventional agarose gel electrophoresis (data not shown), the QIAxcel system enabled unambiguous size estimation in significantly shorter time. • Up to 96 samples can be analyzed in a single run without manual intervention using the QIAxcel system. In addition, the QIAxcel system provides more information from CAPS analyses than traditional methods, saving time and effort. Controlled running conditions and automated data acquisition ensure data safety, reliability, and reproducibility. • QIAxcel capillary electrophoresis uses only minute quantities of DNA for electrokinetic injection, allowing the samples to be used for downstream procedures, such as sequencing or cloning. Lane Estimated fragment size (bp) 1 41 2 180 306 3 210 303 4 179 304 5 179 210 304 6 29 170 300 7 34 171 204 299 8 178 210 303 9 50 100 150 200 250 300 400 500 600 700 800 Table 1. BioCalculator analysis of the gel image in Figure 1 141 2180306 3210303 4179304 5179210304 629170300 734171204299 8178210303 950100150200250300400500600700800

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