Application Guide - QIAxcel Advanced

2 QIAxcel Advanced Application Guide 10/2016 1. gDNA analysis /RNA analysis/NGS library QC 4 Quality control of genomic DNA using the QIAxcel system 6 Quality control of total RNA and cRNA for microarray analysis 10 Identifying rare mutations in Diamond-Blackfan anemia using target enrichment and next-generation sequencing 14 2. DNA analysis – Basic Research 18 A. thaliana genotyping with a CAPS marker for a pks3 mutant allele 20 Detection of alternative Tra2β regulated splicing 24 QIAxcel system — linkage analysis of zebrafish mutants 28 QIAxcel system — mapping mutant gene loci in Arabidopsis thaliana30 Rapid and effective genotyping of Cre transgenic mice 34 3. DNA analysis – Biomedical Research 38 Mutation screening of c-kit and EGFR using the QIAxcel system prior to Pyrosequencing 40 Rapid Analysis of Deletions/Duplications with MLPA Using the QIAxcel Advanced System 44 Rapid and accurate detection of Y chromosome microdeletions 52 Reliable and fast detection of B-cell clonality in lymphoproliferations 56 Safe and rapid HLA typing using the QIAxcel system 60 4. DNA analysis – Microbiology 64 A Novel Approach for Identification of 16 Respiratory Viral Targets 66 Advantages of the QIAxcel system for bacterial genotyping 70 Automated MIRU-VNTR genotyping of Mycobacterium tuberculosis strains using the QIAxcel Advanced Systems 74 ERIC-PCR fingerprinting of indigenous Sinorhizobium meliloti strains78 ISSR-PCR fingerprinting of plant pathogen strains using the QIAxcel system and BioNumerics 82 QIAxcel System — Clostridium difficile ribotype determination 86 5. DNA analysis – Applied Testing 90 Authentication of Basmati rice using SSR-PCR and QIAxcel Advanced 92 Detection of genetically modified plants using the QIAxcel System 98 Fish species identification using PCR-RFLP and the QIAxcel Advanced 102 High-throughput detection of Shiga toxin-producing Escherichia coli using multiplex PCR and the QIAxcel Advanced system 108 Identifying allergenic nut species using the QIAxcel system 116 Identifying meat species using RFLP-PCR and the QIAxcel Advanced system 120 Marker-assisted selection (MAS) of wheat lines for udon noodle production 126 Species determination for meat using PCR-RFLP analysis on the QIAxcel system 130 Chapter Contents

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