Application Guide - QIAxcel Advanced

12 QIAxcel Advanced Application Guide 10/2016 Results The QIAxcel system is a capillary electrophoresis system that processes samples in batches of 12, for analysis of up to 96 samples without manual intervention. Data can be viewed in both gel image and electropherogram format. Intact RNA is a prerequisite for successful microarray analyses. QIAxcel ScreenGel™ software provides reliable analysis of the size, quantity, and quality of total RNA prior to microarray analysis (Figure 2). The two distinct rRNA peaks and 28S/18Speak ratios of 1.48–1.67 indicate the high quality of the total RNA (Figures 2 and 3). Analysis of unlabeled cRNA shows expected profiles with a smear of products concentrated in size from 500 bp to 2000 bp. The size distribution should be equal between samples: less intact RNA yields smaller cRNA fragments (Figure 4). Conclusions These results demonstrate the high suitability of the QIAxcel system for fast, sensitive analysis of the quality and quantity of total RNA and fragmented or intact cRNA. The 28S/18S rRNA ratio, a measure of RNA integrity, was successfully determined — a crucial factor for meaningful gene expression data. The ease of use, fast processing times, and the ability to analyze up to 96 samples without manual intervention make the QIAxcel system particularly well suited for research laboratories with high-throughput needs. 0.2 0 1 2 3 4 5 6 7 8 9 0.4 0.6 0 Migration time (min) (RFU x 1EO) B 0.2 0 1 2 3 4 5 6 7 8 9 0.4 0.6 0 Migration time (min) (RFU x 1EO) A Figure 4. Reliable quality control of unlabeled cRNA generated from yeast total RNA. Results presented as A a gel view and B a superimposed electropherogram view. 0123456789 0123456789

Pages Overview