Application Guide - QIAxcel Advanced

118 QIAxcel Advanced Application Guide 10/2016 Detecting the singleplex and multiplex nut assays and analyzing the data using the QIAxcel ScreenGel® software demonstrated that the QIAxcel system can be used to detect DNA of specific nut species in the foods tested. The specific assays yielded clear results with strong bands corresponding to the targeted fragment sizes. The results of the analysis can also be presented in other formats. 1. A table with up to 16 user-defined parameters, including size, concentration, and percentage. 2. Peak calling tables with information about the presence or absence, size, and concentration of the targeted amplicons in the analyzed samples Conclusions The QIAxcel system can be successfully used to identify PCR products from the DNA of the specific nut varieties almond, hazelnut, macadamia nut, and peanut. Thanks to the fully automated procedure, the results are highly reproducible and reliable. Safety and reproducibility is ensured by defining the process profile, which cannot be changed by a routine user. The process profile defines the electrophoresis parameters, analysis, and reporting of the data. This method used with the QIAxcel DNA High Resolution Kit and QIAxcel system proved to be an excellent tool for identifying nut DNA. These results demonstrate the successful application of the PCR method across a range of food samples and also the high sizing accuracy and reproducibility of results yielded by the QIAxcel electrophoresis system. Table 1. Foods tested for their nut contents with singleplex and multiplex assays using the QIAxcel system Food sample Lane Nuts present on ingredient list Assay Fragment(s) detected (bp) Macadamia cookies 1 Macadamia Macadamia 75 Marzipan 2 Almond Almond 134 Peanut butter 3 Peanut Peanut 99 Brownies 4 May contain nuts Peanut 99 Pistachio baklawa 5 Bakery (Pistachio) Almond 133 Pistachio baklawa 6 Bakery (Pistachio) Hazelnut 110 Dark chocolate 7 Almond, hazelnut Almond 133 Dark chocolate 8 Almond, hazelnut Hazelnut 110 Cereal bars 9 Almond, peanut, hazelnut Almond 134 Cereal bars 10 Almond, peanut, hazelnut Hazelnut 110 Cereal bars 11 Hazelnut Peanut 98 Marzipan 12 Almond Multiplex 134 Peanut butter 13 Peanut Multiplex 99 Brownies 14 May contain nuts Multiplex 99 Dark chocolate 15 Almond and hazelnut Multiplex 110/134 Cereal bars 16 Almond, peanut, hazelnut Multiplex 99/110/134 Pistachio baklawa 17 Bakery (Pistachio) Multiplex 98/134

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