Application Guide - QIAxcel Advanced

124 QIAxcel Advanced Application Guide 10/2016 Figure 3. An electropherogram view of the data for the analyzed samples. A. Sample FF2 digested with AluI (corresponding to Lane A6 in Figure 2). B. Sample FF2 digested with HinfI (corresponding to Lane C3 in Figure 2). 0 15 50 75 100 150 200 15 bp 15 bp 49 bp 49 bp 121 bp 121 bp 203 bp 203 bp 366 bp 366 bp 600 bp 600 bp 250 300 400 500 [bp] 0.6 0.4 0.5 0.3 0.2 0.1 RFU x 1E0 Size 0 15 50 75 100 150 200 15 bp 15 bp 113 bp 113 bp 169 bp 169 bp 190 bp 190 bp 245 bp 245 bp 600 bp 600 bp 250 300 400 500 [bp] 1.4 1.2 1.0 0.8 0.6 0.4 0.2 RFU x 1E0 Size 0 15 50 75 100 150 200 15 bp 15 bp 49 bp 49 bp 121 bp 121 bp 203 bp 203 bp 366 bp 366 bp 600 bp 600 bp 250 300 400 500 [bp] 0.6 0.4 0.5 0.3 0.2 0.1 RFU x 1E0 Size 0 15 50 75 100 150 200 15 bp 15 bp 113 bp 113 bp 169 bp 169 bp 245 bp 245 bp 600 bp 600 bp 250 300 400 500 [bp] 1.2 1.0 0.8 0.6 0.4 0.2 Size In this study, four out of six samples were found to be correctly labeled with the RFLP-PCR yielding the expected results. However, we found that samples FF2 and FF3 were not properly labeled: they contained various amounts of pork despite being labeled as pure beef. All of the commercial samples analyzed had undergone different production processes. The results indicated that the enzyme digestions were incomplete in some samples (lanes A5 to A9, Figure 2). Therefore, the samples were additionally analyzed with real-time PCR (results not shown), which confirmed that the digestion with AluI was incomplete for those samples. Conclusion • The QIAxcel Advanced System facilitates analysis of PCR- RFLP samples for meat species identification, especially for large-scale analyses. • Using this native capillary electrophoresis system significantly reduces analysis time for the authentication of meat samples and minimizes the potential for procedural errors that would influence the accuracy of analysis. • The method also eliminates exposure to hazardous chemicals and is easy to handle, making it an excellent routine method for the control of meat and meat products. A B 155075100150200 250300400500 [bp] 155075100150200 250300400500 [bp] 155075100150200 250300400500 [bp] 155075100150200 250300400500 [bp]

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