Application Guide - QIAxcel Advanced

8 QIAxcel Advanced Application Guide 10/2016 A B Figure 2. Assessment of DNA degraded by sonication. A Agarose gel photo showing gDNA in various states of degradation. Lane 1 non- degraded gDNA samples. Lanes 2 and 3: gDNA samples partially degraded by sonication with 7 and 14 pulses, respectively. Lanes 4 and 5: gDNA samples fully degraded with 70 and 105 pulses, respectively. B QIAxcel gel photo showing gDNA samples in three states of degradation. 1, 3, and 5 correspond to lanes 1, 3, and 5 in A. C Electropherograms and a gel photo of gDNA samples from lanes 1, 3, and 5 in A. Lane 1: Electropherogram indicates very good quality gDNA (no degradation products before the major peak, long tailing off). Lane 3: Partially degraded gDNA (some degradation product signals, no tailing off). Lane 5: Highly degraded gDNA (many degradation product signals, no tailing off). 1 2 3 4 5 1 3 5 Conclusions Quality control of genomic DNA purified with silica membrane-based methods can routinely and effectively be performed using the QIAxcel system. The optimal procedure uses the DNA Screening Cartridge and the AM900 method. The results are highly reproducible, as shown in repeated runs. Using the electropherogram data as well as the results from a gel image allows straightforward verification of the integrity of the purified gDNA. If degradation products are present, they are visible as a broad peak preceding the major gDNA peak. In order to detect even minute quantities of unwanted degradation products, a long sample injection time of 40 seconds is suitable. It is recommended that gDNA concentrations ranging between 25 and 100 ng/μl are used. This novel method using the QIAxcel system is optimal for gDNA quality control. C 12345135

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