Application Guide - QIAxcel Advanced

96 QIAxcel Advanced Application Guide 10/2016 References 1. British Retail Consortium (2005) Code of Practice on Basmati Rice. www.brc.org.uk/Downloads/Basmati_Code.pdf 2. Archak S., Lakshminarayanareddy, V. and Nagaraju, J. (2007) High-throughput multiplex microsatellite marker assay for detection and quantification of adulteration in Basmati rice (Oryza sativa). Electrophoresis 28, 2396. 3. Vemireddy L.R., Archak, S. and Nagaraju, J. (2007) Capillary electrophoresis is essential for microsatellite marker based detection and quantification of adulteration of Basmati rice (Oryza sativa). J Agric Food Chem 55, 8112. 4. Steele K. (2009) Rice Authentication using SSR and InDel markers. Online presentation. www.ec.europa.eu/agriculture/analysis/external/basmati/ssr_markers_steele_en.pdf Conclusions • The SSR-PCR protocol for the QIAxcel Advanced System combined with a SSR marker allele database for rice proved to be accurate, reliable and fast, making this experimental setup a good candidate for routine Basmati rice authentication analysis. • Identification and quantification of Basmati rice and its adulterants was successfully performed using the QIAxcel High Resolution Kit, which discriminates SSR fragment sizes with an accuracy of 3–5 bp. • The method is best suited for samples containing 1 or 2 different rice varieties. Analysis of samples with 3 or more different rice types is more complex and requires additional testing. • The method detects adulterant contents as low as 0.1%, with quantification of up to 99.0% Basmati rice. The overall uncertainty is 5.2%, and only 1.9% for mixtures with a Basmati rice proportion higher than 95%.

Pages Overview