Application Guide - QIAxcel Advanced

QIAxcel Advanced Application Guide 10/2016 47 The peak height ratio between deleted (Figure 2) or duplicated (Figure 3) exon probes with their corresponding reference probes showed changes in the specific target region. APC deletion Q-64 (DNA) 66 bp 85 bp 93 bp 136 bp 178 bp 196 bp 201 bp 220 bp 228 bp 246 bp 253 bp 264 bp 270 bp 283 bp 304 bp 289 bp 311 bp 297 bp 319 bp 330 bp 333 bp 354 bp 365 bp 367 bp 371 bp 379 bp 388 bp 399 bp 389 bp 426 bp 408 bp 454 bp 448 bp Q-70 (DNA) Q-82 (DNA) CARM1-1 (DD) JPH3-up (DD) IL1B-4 (Lig) UTY-15 (YC) MUTYH-9 APC-2 APC-2 APC-3 APC-4 APC-5 APC-6 APC-7 APC-8 APC-9 APC-1 APC-1 APC-10 APC-11 APC-12 APC-13 APC-14 APC-15 APC-16 APC-18 APC-18 APC-18 APC-18 (WT) APC-18 (WT) APC-intr.13 APC-17 MUTYH-1 MUTYH-4 AMOT-4 (XC) Reference* Reference* Reference* Reference* Reference* Reference* Reference* Reference* Reference* Reference* Q-80 (DNA) Relative fluorescence units 64 70 82 80 88 92 96 100 105 130 136 142 150 157 171 178 186 193 202 211 220 229 238 216 253 260 267 274 282 289 297 306 312 321 328 337 346 356 365 373 382 391 400 409 418 427 445 A Relative fluorescence units (RFU x 1EO) B 0.120 0.110 0.100 0.090 0.080 0.070 0.060 0.050 0.040 0.030 0.020 0.010 0.000 5000 4500 4000 3500 3000 2500 2000 1500 1000 5000 0 Figure 2. Comparable pattern of peaks for APC deletion using sequencing and QIAxcel Advanced System amplicon visualization techniques. A Electropherogram from Coffalyser software analysis of sequencing. B Electropherogram from the QIAxcel Screen Gel 1.2 software analysis of QIAxcel Advanced System capillary electrophoresis. Green circles show reference peaks. Black arrows indicate APC peaks reduced compared with the corresponding APC control electropherograms in Figure 1. Arrows are similarly placed between electropherograms A and B.

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