Application Guide - QIAxcel Advanced

46 QIAxcel Advanced Application Guide 10/2016 Results Electropherograms Electropherograms from the Coffalyser software analyses showed peak patterns comparable to the corresponding electropherograms from QIAxcel ScreenGel 1.2 analyses (Figures 1–3). The electropherograms represent typical samples from the APC MLPA analyses. Figure 1. Comparable pattern of peaks for APC control using an ABI PRISM® 3100 Genetic Analyzer and QIAxcel Advanced System. A Electropherogram from Coffalyser software analysis. B Electropherogram from the QIAxcel Screen Gel 1.2 software analysis. Green circles show reference peaks. APC control 64 Q-64 (DNA) 135 bp 157 bp 163 bp 178 bp 185 bp 195 bp 203 bp 210 bp 219 bp 223 bp 250 bp 253 bp 254 bp 274 bp 270 bp 296 bp 307 bp 319 bp 330 bp 333 bp 334 bp 353 bp 365 bp 358 bp 368 bp 371 bp 379 bp 382 bp 387 bp 399 bp 396 bp 425 bp 419 bp 420 bp 455 bp 458 bp Q-70 (DNA) Q-82 (DNA) CARM1-1 (DD) JPH3-up (DD) IL1B-4 (Lig) UTY-15 (YC) MUTYH-9 APC-2 APC-3 APC-4 APC-5 APC-6 APC-7 APC-8 APC-9 APC-1 APC-1 APC-10 APC-11 APC-12 APC-13 APC-14 APC-15 APC-16 APC-18 APC-18 APC-18 APC-18 (WT) APC-18 (WT) APC-intr.13 APC-17 MUTYH-1 MUTYH-4 APC-2 AMOT-4 (XC) Reference* Reference* Reference* Reference* Reference* Reference* Reference* Reference* Reference* Reference* Q-80 (DNA) 70 82 80 88 92 96 100 105 130 136 142 150 157 171 178 186 193 202 211 220 229 238 216 253 260 267 274 282 289 297 306 312 321 328 337 346 356 365 373 382 391 400 409 418 427 445 Relative fluorescence units A Relative fluorescence units B 0.150 0.140 0.130 0.120 0.110 0.100 0.090 0.080 0.070 0.060 0.050 0.040 0.030 0.020 0.010 0.000 4800 4600 4400 4200 4000 3800 3600 3400 3200 3000 2800 2600 2400 2200 2000 1800 1600 1400 1200 1000 800 600 400 200 0

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