Product FAQ - QIAGEN



Product FAQ Bookmark Share more.. You are not authorized to download the resource Sort options Sort alphabetically Sort by relevance Sorted alphabetically Do you have a protocol for evaluating temperature accuracy of the BioRobot EZ1? FAQ ID -1168 View Do you have a protocol for GMO testing of food samples? FAQ ID -921 View Do you have a protocol for high-throughput purification of plasmid DNA using the BioRobot Universal System? FAQ ID -1191 View Do you have a protocol for isolating cytoplasmic RNA from animal cells using the RNeasy Mini Kit? FAQ ID -1207 View Do you have a protocol for isolating RNA from yeast using RNeasy? FAQ ID -535 View Do you have a protocol for isolation of bacterial DNA from soil? FAQ ID -923 View Do you have a protocol for isolation of genomic and plasmid DNA from cell cultures using QIAGEN Genomic-tips? FAQ ID -902 View Do you have a protocol for isolation of genomic and viral DNA from lymphocytes using QIAGEN Genomic-tips? FAQ ID -901 View Do you have a protocol for isolation of genomic DNA from dried blood spots using the QIAamp 96 DNA Blood Kit? FAQ ID -919 View Do you have a protocol for isolation of genomic DNA from flies using QIAGEN Genomic-tips? FAQ ID -903 View Do you have a protocol for isolation of genomic DNA from insects? FAQ ID -904 View Do you have a protocol for isolation of genomic DNA from plants and filamentous fungi using QIAGEN Genomic-tips? FAQ ID -905 View Do you have a protocol for isolation of genomic DNA from saliva and mouthwash? FAQ ID -917 View Do you have a protocol for isolation of genomic DNA from saliva using the DNeasy Blood & Tissue Kit? FAQ ID -931 View Do you have a protocol for isolation of plasmid DNA using the Sigma Centrifuge 4? -15 View Do you have a protocol for long-term, siRNA-mediated gene silencing using HiPerFect Transfection Reagent? FAQ ID -972 View Do you have a protocol for manual purification of 6xHis-tagged proteins expressed in E. coli using Ni-NTA Superflow? FAQ ID -967 View Do you have a protocol for obtaining > 20 µg plasmid DNA using the QIAprep Spin Miniprep Kit? FAQ ID -3102 View Do you have a protocol for performing quantitative multiplex PCR on the LightCycler 2.0 system? FAQ ID -966 View Do you have a protocol for polyacrylamide gel analysis of oligonucleotides? FAQ ID -961 View Do you have a protocol for purification of cytoplasmic RNA from animal cells? FAQ ID -1257 View Do you have a protocol for purification of DNA fragments from Cy3/Cy5 dye-label reactions? FAQ ID -947 View Do you have a protocol for purification of DNA from clotted blood using the FlexiGene DNA Kit? FAQ ID -973 View Do you have a protocol for purification of DNA from cultured animal cells using the DNeasy 96 Blood & Tissue Kit? FAQ ID -934 View Do you have a protocol for purification of DNA from stool samples for pathogen detection using BioSprint Instruments? FAQ ID -1165 View Do you have a protocol for purification of DNA from stool samples using BioSprint Instruments? FAQ ID -1166 View Do you have a protocol for purification of genomic DNA from cultured cells using the QIAamp DNA Micro Kit? FAQ ID -1547 View Do you have a protocol for purification of miRNA from animal cells using the RNeasy Plus Mini Kit and RNeasy MinElute Cleanup Kit? FAQ ID -1258 View Do you have a protocol for purification of total DNA from crude lysates? FAQ ID -1255 View Do you have a protocol for purification of total DNA from insects? FAQ ID -1254 View Do you have a protocol for purification of total DNA from yeast? FAQ ID -1253 View Do you have a protocol for purification of total RNA from fatty tissues using QIAzol Lysis Reagent and MaXtract High Density? FAQ ID -1550 View Do you have a protocol for purification of total RNA from plant latex? FAQ ID -1694 View Do you have a protocol for purification of total RNA from plant tissue with the RNeasy Lipid Tissue Mini Kit? FAQ ID -1693 View Do you have a protocol for purifying 2x96 PCR samples simultaneously with the QIAquick 96 PCR Purification Kit? FAQ ID -935 View Do you have a protocol for radioactive labelling of cDNA? FAQ ID -960 View Do you have a protocol for rapid purification of archive-quality DNA from up to 9 x 10e6 cells using Gentra Puregene Kits? FAQ ID -1969 View Do you have a protocol for reverse transfection of adherent cells with siRNA in 384-well plates? FAQ ID -971 View Do you have a protocol for Rotor-Gene software setup for the Rotor-Gene SYBR Green PCR and RT-PCR Kits? FAQ ID -2104 View Do you have a protocol for Streptokinase treatment of clotted blood? FAQ ID -912 View Do you have a protocol for the AllPrep DNA/RNA/Protein Mini Kit on the QIAcube Connect? FAQ ID - 141506 View Do you have a protocol for the detection of Bordetella pertussis DNA by PCR? FAQ ID -914 View Do you have a protocol for the fixation of cells transfected with fluorescently labeled siRNA? FAQ ID -793 View Do you have a protocol for the isolation of BAC DNA using the QIAGEN Plasmid Midi Kit? FAQ ID -881 View Do you have a protocol for the isolation of bacteriophage P1 derived constructs with any of your plasmid kits? FAQ ID -883 View Do you have a protocol for the isolation of DNA from buccal cells using the BioRobot EZ1? FAQ ID -1260 View Do you have a protocol for the isolation of DNA from buffy coat using the BioRobot EZ1? FAQ ID -985 View Do you have a protocol for the isolation of DNA from cultured cells using the BioRobot EZ1? FAQ ID -990 View Do you have a protocol for the isolation of DNA from epithelial cells mixed with sperm cells? FAQ ID -926 View Do you have a protocol for the isolation of DNA from forensic samples using the BioRobot M48? FAQ ID -1017 View Do you have a protocol for the isolation of DNA from formalin-preserved stool samples? FAQ ID -922 View Do you have a protocol for the isolation of DNA from fungi using the BioRobot EZ1? FAQ ID -992 View Do you have a protocol for the isolation of DNA from mouse tails using MagAttract technology on BioRobot M96? FAQ ID -1007 View Do you have a protocol for the isolation of DNA from soft tissues using the TissueLyser? FAQ ID -924 View Do you have a protocol for the isolation of DNA from tofu? FAQ ID -932 View Do you have a protocol for the isolation of genomic DNA and/or proteins from fatty tissue treated with QIAzol? FAQ ID -955 View Do you have a protocol for the isolation of genomic DNA from bone? FAQ ID -908 View Do you have a protocol for the isolation of genomic DNA from frozen clotted blood? FAQ ID -900. Test View Do you have a protocol for the isolation of genomic DNA from fungi? FAQ ID -911 View Do you have a protocol for the isolation of genomic DNA from paraffin-embedded samples on the BioRobot M48? FAQ ID -1019 View Do you have a protocol for the isolation of genomic DNA from sperm? FAQ ID -909 View Do you have a protocol for the isolation of genomic DNA from whole blood for use in MRC-Holland MLPA® assays? FAQ ID -1169 View Do you have a protocol for the isolation of genomic DNA from yeast/fungi directly from Blood? FAQ ID -3150 View Do you have a protocol for the isolation of plasmid DNA from Agrobacterium? FAQ ID -898 View Do you have a protocol for the isolation of plasmid DNA from Bacillus subtilis? FAQ ID -896 View Do you have a protocol for the isolation of plasmid DNA from Borrelia spp.? FAQ ID -884 View Do you have a protocol for the isolation of plasmid DNA from Citrobacter freundii? FAQ ID -885 View Do you have a protocol for the isolation of plasmid DNA from Corynebacterium glutamicum? FAQ ID -886 View Do you have a protocol for the isolation of plasmid DNA from Lactobacillus spp.? FAQ ID -887 View Do you have a protocol for the isolation of plasmid DNA from Oligotropha carboxidovorans? FAQ ID -888 View Do you have a protocol for the isolation of plasmid DNA from Proteus spp.? FAQ ID -889 View Do you have a protocol for the isolation of plasmid DNA from Staphylococcus spp.? FAQ ID -890 View Do you have a protocol for the isolation of plasmid DNA from yeast? FAQ ID -853 View Do you have a protocol for the isolation of RNA from bacterial cultures using RNAprotect Bacteria Reagent and QIAzol Lysis Reagent? FAQ ID -1020 View Do you have a protocol for the isolation of RNA from leukocytes in milk? FAQ ID -952 View Do you have a protocol for the isolation of single-stranded DNA from M13 phage using QIAGEN Plasmid Kits? FAQ ID -894 View Do you have a protocol for the isolation of total nucleic acids from animal and human cells on the BioRobot EZ1? FAQ ID -974 View Do you have a protocol for the isolation of total nucleic acids from animal and human tissues on the BioRobot EZ1? FAQ ID -975 View Do you have a protocol for the isolation of total nucleic acids from cells on the M48 BioRobot? FAQ ID -1009 View Do you have a protocol for the isolation of total nucleic acids from tissue on the M48 BioRobot? FAQ ID -1008 View Do you have a protocol for the isolation of total RNA from buccal swabs? FAQ ID -956 View Do you have a protocol for the isolation of total RNA from paraffin-embedded sections on the BioRobot EZ1? FAQ ID -976 View Do you have a protocol for the isolation of total RNA from paraffin-embedded sections on the Biorobot M48? FAQ ID -1733 View Do you have a protocol for the isolation of total RNA from plant tissue using QIAGEN-tips? FAQ ID -949 View Do you have a protocol for the isolation of total RNA from plants using the RNeasy 96 Kit? FAQ ID -951 View Do you have a protocol for the isolation of very low-copy plasmids from Streptomyces spp.? FAQ ID -893 View Do you have a protocol for the isolation of viral DNA from stool? FAQ ID -929 View Do you have a protocol for the isolation of viral RNA from stool? FAQ ID -918 View Do you have a protocol for the preparation of tissues preserved in RNAprotect Tissue Reagent for pathological studies? FAQ ID -950 View Do you have a protocol for the purification of 6xHis-tagged proteins using BioSprint? FAQ ID -1167 View Do you have a protocol for the purification of archive-quality DNA from 0.5–20 mg paraffin-embedded tissue using Gentra Puregene Kits? FAQ ID -1964 View Do you have a protocol for the purification of archive-quality DNA from 1 ml of whole blood with the Gentra Puregene Tissue Kit? FAQ ID -1953 View Do you have a protocol for the purification of archive-quality DNA from 100 to 5 x 10e8 cultured cells using Gentra Puregene Kits? FAQ ID -1960 View Do you have a protocol for the purification of archive-quality DNA from blood smears using Gentra Puregene Kits? FAQ ID -1948 View Do you have a protocol for the purification of archive-quality DNA from buccal brushes using Gentra Puregene Kits? FAQ ID -1965 View Do you have a protocol for the purification of archive-quality DNA from buffy coat using Gentra Puregene Kits? FAQ ID -1955 View Do you have a protocol for the purification of archive-quality DNA from cerebrospinal fluid (CSF) using Gentra Puregene Kits? FAQ ID -1967 View Do you have a protocol for the purification of archive-quality DNA from Chlamydomonas using Gentra Puregene Kits? FAQ ID -1968 View Do you have a protocol for the purification of archive-quality DNA from Eimeria oocysts using Gentra Puregene Kits? FAQ ID -1952 View Do you have a protocol for the purification of archive-quality DNA from fecal cells using Gentra Puregene Kits? FAQ ID -1972 View Do you have a protocol for the purification of archive-quality DNA from fish using Gentra Puregene Kits? FAQ ID -1973 View Do you have a protocol for the purification of archive-quality DNA from fungus using Gentra Puregene Kits? FAQ ID -1971 View Do you have a protocol for the purification of archive-quality DNA from Gram-negative bacteria using Gentra Puregene Kits? FAQ ID -1975 View Do you have a protocol for the purification of archive-quality DNA from Gram-positive bacteria using Gentra Puregene Kits? FAQ ID -1946 View Do you have a protocol for the purification of archive-quality DNA from hair roots using Gentra Puregene Kits? FAQ ID -1947 View Do you have a protocol for the purification of archive-quality DNA from human dried blood using Gentra Puregene Kits? FAQ ID -1959 View Do you have a protocol for the purification of archive-quality DNA from marine invertebrate tissue using Gentra Puregene Kits? FAQ ID -1950 View Do you have a protocol for the purification of archive-quality DNA from nematodes using Gentra Puregene Kits? FAQ ID -1951 View Do you have a protocol for the purification of archive-quality DNA from tissue fixed in ethanol or formalin using Gentra Puregene Kits? FAQ ID -1974 View Do you have a protocol for the purification of archive-quality DNA from umbilical cord blood using Gentra Puregene Kits? FAQ ID -1958 View Do you have a protocol for the purification of archive-quality DNA from whole blood using Gentra Puregene Kits? FAQ ID -1954 View Do you have a protocol for the purification of DNA from buccal swabs using the BioSprint System? FAQ ID -983 View Do you have a protocol for the purification of DNA from cells using the BioSprint System? FAQ ID -977 View Do you have a protocol for the purification of DNA from dried blood spots using the BioSprint System? FAQ ID -982 View Do you have a protocol for the purification of DNA from fruit flies? FAQ ID -1970 View Do you have a protocol for the purification of DNA from rodent tail using the BioSprint System? FAQ ID -979 View Do you have a protocol for the purification of PCR products using the BioSprint System? FAQ ID -984 View Do you have a protocol for the QIAprep Spin M13 Kit on the QIAcube Connect? FAQ ID - 1414507 View Do you have a protocol for the removal of endotoxins from already purified plasmid DNA? FAQ ID -500 View Do you have a protocol for transfection of adherent cells with low concentrations of siRNA using RNAiFect Transfection Reagent? FAQ ID -970 View Do you have a protocol for transfection of differentiated macrophage cell lines (THP) with siRNA? -1 View Do you have a protocol for transfection of macrophage cell lines (J774.A1 and RAW 264.7) with siRNA? FAQ ID -1251 View Do you have a protocol for transfection of suspension cell lines (Jurkat and K562) with siRNA? FAQ ID -1250 View Do you have a protocol for transient transfection of cells in 96-well plates using PolyFect Transfection Reagent? FAQ ID -1013 View Do you have a protocol for transient transfection of HEK 293 cells in 75 cm3 flasks? FAQ ID -1014 View Do you have a protocol for transient transfection of HeLa-S3 cells in 60 mm dishes using PolyFect? FAQ ID -1012 View Do you have a protocol for transient transfection of Hela-S3 cells using PolyFect Transfection Reagent? FAQ ID -997 View Do you have a protocol for vacuum processing of blood samples with the QIAamp DNA Blood Midi or Maxi Kits? FAQ ID -1011 View Do you have a protocol to isolate RNA from blood and bone marrow using the BioRobot EZ1? FAQ ID -567 View Do you have an antibody for Western Blot Analysis of MAPK1 gene knockdown using your MAPK1 control siRNA? FAQ ID -746 View Do you have an in vitro translation kit allowing the incorporation of seleno-methionine for X-Ray crystallography based protein analysis? FAQ ID -879 View Do you have an off-the shelf diagnostics kit for WGA? FAQ ID -678 View Do you have any information or guidelines regarding the choice of reference genes for real-time PCR? FAQ ID -2371 View Do you have any recommendations for using the PosphoProtein Kit with starting material other than cells? FAQ ID -597 View Do you have any recommendations on how to process my AAV samples? FAQ ID - 3860 View Do you have data on the DNA yield at different incubation time for the Repli-G Kits? FAQ ID -3113 View Do you have data showing effects of sample size on DNA yield and purity using the DNeasy 96 Blood & Tissue kit? FAQ ID -129 View Do you have data to show lowest dropout rate with Repli-G WTA Single Cells kit? FAQ ID - 3392 View Do you have FACS or Fluoro-Microscopy data using HiPerFect HTS Reagent for delivery of siRNA? FAQ ID -2557 View Do you have information about the cleanup of single-stranded DNA (ssDNA) with QIAquick columns? FAQ ID -759 View Do you have information on DNA yields from blood with variable numbers of white blood cells using the M48 BioRobot? FAQ ID -1006 View Do you have information on in vivo RNA interference experiments? FAQ ID -817 View Do you have information on PCR template preparation from preserved blood samples on the M48 BioRobot? FAQ ID - 1003 View Do you have information on realtime RT-PCR performance of RNA isolated from monocytes on the M48 BioRobot? FAQ ID -1010 View Do you have information on the reproducibility of DNA yields from blood using the M48 BioRobot? FAQ ID - 1004 View Do you have information on the use of recombinant DNA and RNA as absolute standards for realtime RT-PCR? FAQ ID -729 View Do you have protocols for multiple extractions of DNA fragments from agarose gels? FAQ ID -944 View Do you have protocols for sample processing on the Autopure LS instrument? FAQ ID -1759 View Do you have protocols for software setup on various PCR platforms using QuantiFast Probe RT-PCR Kits? FAQ ID -2105 View Do you have protocols for the isolation of genomic DNA from tissue using QIAGEN-tips 2500 (Mega) and 10000 (Giga)? FAQ ID -907 View Do you have stability data for genomic DNA isolated with the BioSprint Kits? FAQ ID -1318 View Do you have stability data for genomic DNA isolated with the Flexigene DNA Kit? FAQ ID -1317 View Do you have stability data for genomic DNA isolated with the QIAamp DNA Blood Mini Kit? FAQ ID -518 View Do you have stability data for RNA isolated with PAXgene miRNA Kit? FAQ ID - 3480 View Do you have stability data for RNA isolated with the QIAsymphony PAXgene Blood RNA Kit? FAQ ID -2983 View Do you have to use a restriction enzyme to relieve ITR secondary structures and increase target accessibility? FAQ ID - 3974 View Do you have transfection data for QIAGEN Transfection Reagents? FAQ ID -158 View Do you have WGA protocols for starting materials not mentioned in the REPLI-g Handbooks? FAQ ID -1160 View Do you know of anything in the buffers/DNA sample that can cause bias in REPLI-g WGA? FAQ ID -684 View Do you need to dilute the lysates before setting up the PCR mix? FAQ ID - 3978 View Do you need to pre-treat food samples before DNA isolation using the QIAsymphony mericon Bacteria Kit on the QIAsymphony SP? FAQ ID -2957 View Do you offer a QuantiFast Kit for one-step RT-PCR? FAQ ID -1695 View Do you offer FlexiTube or FlexiPlate siRNAs for rat? FAQ ID -3114 View Do you offer QIAshredder spin columns for the RNeasy Midi/Maxi Kit format? If not, what is the alternative? FAQ ID -560 View Do you offer trial-kit sizes for the new QuantiFast Kits? FAQ ID -1429 View Do you provide control proteins for use with the PhosphoProtein Purification Kit? FAQ ID -596 View Do you recommend 1-step or 2-step real-time RT-PCR for gene expression analysis? FAQ ID -1056 View Do you recommend normalizing single cell miRNA expression data to a “housekeeping” gene or miRNA? FAQ ID - 3580 View Do you recommend storage of highly diluted AAV samples? FAQ ID - 3972 View Do you recommend storage of highly diluted mycoplasma samples? FAQ ID - 3998 View Do you recommend using indicator cards for buccal cells on FTA with Investigator IDplex GO! Kit? FAQ ID -3095 View Do you sell CoralLoad dye separately? FAQ ID - 3368 View Do you sell the ATP-Dependent Exonuclease of the QIAGEN Large-Construct Kit separately? FAQ ID -825 View Do you synthesize thiophosphate modified random hexamers? FAQ ID -711 View Does a QIAseq miRNA library include hY4 Y RNA? FAQ ID - 3670 View Does an activation time of 15 minutes influence the performance of the HotStarTaq Plus DNA Polymerase? FAQ ID -1050 View Does an internal control have to be included for each QIAsymphony Virus/Bacteria protocol run? FAQ ID -2040 View Does an internal control have to be used with the QIAsymphony Virus Blood 200 Protocol? FAQ ID -2162 View Does anything have to be added to express post-translational modifications (PTMs) with the EasyXpress Insect Kit II? FAQ ID -1226 View Does Buffer APP in the AllPrep DNA/RNA/Protein Mini Kit contain acetone? FAQ ID -1576 View Does carrier RNA have to be added using the QIAsymphony Virus Blood 200 Protocol? FAQ ID -2163 View Does carrier RNA interfere with the QuantiTect Whole Transcriptome amplification? FAQ ID -3080 View Does cDNA amplified with the QuantiTect Whole Transcriptome Kit correspond to full-length RNA transcripts? FAQ ID -1585 View Does DEPC harm RNA? FAQ ID -529 View Does endogenously expressed DAPase in human cells degrade proteins that are expressed in cell culture? FAQ ID -527 View Does ethanol from the PCR product purification influence the normalization procedure for the SeqTarget System? FAQ ID -2244 View Does GelPilot DNA Loading Dye contain ethidium bromide? FAQ ID -1286 View Does HiPerFect work for the transfection of miScript miRNA mimics and inhibitors? FAQ ID -1984 View Does QIAGEN guarantee the performance of the SureSilencing shRNA Plasmids? FAQ ID -2787 View Does QIAGEN offer a design of Custom PyroMark CpG Assays? FAQ ID -2818 View Does QIAGEN offer an anti-GFP gapmer control? FAQ ID - 3739 View Does QIAGEN offer vectors for direct cloning of PCR products? FAQ ID -146 View Does QIAGEN offers arrays and assays configured specifically for QIAquant instrument? 153929 View Does QIAGEN provide template files for GeneMapper® 5 software? FAQ ID -3177 View Does QIAGEN sell 6xHis-tagged proteins besides the 6xHis Protein Ladder? FAQ ID -594 View Does QIAGEN sell a vector with a T7 promotor for use with the EasyXpress Protein Synthesis Insect Kit? FAQ ID -937 View Does QIAGEN sell Q-Solution separately? FAQ ID -204 View Does REPLI-g technology for whole genome amplification work with paraffin embedded samples? FAQ ID -673 View Does residual Allprotect Tissue Reagent left behind on tissue samples interfere with DNA, RNA, and protein purification? FAQ ID -1573 View Does RNAprotect affect morphology of tissues? FAQ ID -3142 View Does RNase-free DNase I affect the isolation of dsRNA? FAQ ID -3153 View Does salt concentration in the food matter when using the DNeasy mericon Food Kit? FAQ ID - 3346 View Does SYBR Green dye present in QuantiTect kits interfere with ethidium bromide staining? FAQ ID -312 View Does the 5x OneStep RT-PCR Buffer contain BSA? FAQ ID -326 View Does the addition of sample and organic solution to the MaXtract Tubes require a specific pipetting sequence? FAQ ID -1307 View Does the amount of Internal Control to be spiked into the lysis buffer or lysate depend on the elution volume only? FAQ ID -2604 View Does the atypical look of the lyophilized FlexiTube siRNA Premix have a negative impact on performance? FAQ ID -2267 View Does the centrifugal force have any effect on the kinetics of a PCR reaction on the Rotor-Gene Q? FAQ ID -2081 View Does the DNA extracted using the PAXgene Blood DNA System contain RNA? FAQ ID - 3499 View Does the EasyXtal Tool conform to an SBS format? FAQ ID -1115 View Does the Epitect Bisulfite Kit also work on DNA extracted from plants? FAQ ID -1209 View Does the high primer concentration required by QuantiFast SYBR Green PCR Kits impair annealing specificity? FAQ ID -1444 View Does the His-tag interfere with the purification procedure of the QIAGEN PhosphoProtein Purification Kit? FAQ ID -598 View Does the insert sequence provided in the specification sheet correspond to the target sequence or to anti-sense sequence? FAQ ID - 3627 View Does the KRAS PCR kit cover the codon 61 mutation? FAQ ID -2308 View Does the master mix in the QuantiFast Kits contain dUTP to allow UNG treatments? FAQ ID -1431 View Does the miScript Plant RT Kit contain controls? FAQ ID - 3435 View Does the miScript Single Cell qPCR Kit contain controls? FAQ ID - 3574 View Does the miScript Target Protector match the target sequence? FAQ ID -2250 View Does the PAXgene Tissue RNA Kit co-purify small RNAs? FAQ ID -3048 View Does the PAXgene Tissue RNA Kit co-purify small RNAs? FAQ ID - 3615 View Does the PyroMark Assay Design or application software give any cycling conditions for individual assay primers or a PCR setup pipetting scheme? FAQ ID -2862 View Does the PyroMark CpG LINE assay target mouse transposons as well? -1 View Does the PyroMark LINE assay target all LINE sequences? 2860 View Does the QIAquant Software have the capacity to export data? Which data formats can be used for the export? 153946 View Does the QIAsymphony mericon Bacteria Kit contain an extraction control? FAQ ID -2961 View Does the QIAsymphony PAXgene Blood ccfDNA Kit also allow eluting ccfDNA in less than 60 µl? FAQ ID - 3656 View Does the QIAsymphony SP system generate run reports and/or log files? FAQ ID -1911 View Does the QuantiTect Whole Transcriptome Kit work with RNA purified from bacteria, yeast, or plants? FAQ ID -1615 View Does the REPLI-g Single Cell DNA Library Kit include a size selection step? FAQ ID - 3539 View Does the REPLI-g Single Cell DNA Library Kit include adapters? FAQ ID - 3538 View Does the REPLI-g Single Cell RNA Library Kit include a size selection step? FAQ ID - 3551 View Does the REPLI-g Single Cell RNA Library Kit include adapters? FAQ ID - 3550 View Does the reuse of the Waste Tubes increase the risk of cross-contamination? FAQ ID - 3988 View Does the Rotor-Gene Q software have the capacity to export data? FAQ ID -2089 View Does the same U1 promoter in the SureSilencing shRNA Plasmids express shRNA in human, mouse, and rat cell lines? FAQ ID -2887 View Does the Type-it Microsatellite PCR Kit always require an annealing temperature of 60°C, or can pre-optimized annealing temperatures for target-primer systems be used? FAQ ID -2061 View Does the use of MAPK1 as a control in siRNA transfection experiments have secondary effects on the cell culture? FAQ ID -548 View Does this method with the EpiTect Methyl qPCR Array detect methylation at specific CpG dinucleotides? FAQ ID -2737 View Does TopTaq Stabilizer of the TopTaq DNA Polymerase and Master Mix Kits interfere with downstream applications? FAQ ID -1744 View dPCR results are in copies per microliter. How do I calculate host cell DNA contamination in femtogram per microliter? FAQ ID - 3876 View During data analysis, how should the threshold be set in the Yellow channel on the Rotor-Gene Q cycler to analyze the Internal Control from the QuantiFast Pathogen PCR +IC Kit or the QuantiFast Pathogen RT-PCR +IC Kit FAQ ID -2607 View Following thermal cycling, reaction volume has evaporated from some of the wells of my qPCR assay plate. What should I do? FAQ ID -2683 View For how long are BioMag Particles stable? FAQ ID -262 View For sequencing application, does the lysate require purification? 3792 View For the QuantiFast Pathogen RT-PCR +IC kit, does the Internal Control also control for the reverse transcription reaction? FAQ ID -2601 View For the Sanger analysis tool, the customer needs to upload two .abi1 files of forward and reverse sequences? Is there any additional info that needs to be provided? 3796 View For the the QIAprep 96 Plus Miniprep Kit or the QIAGEN Plasmid Plus 96 Miniprep Kit, can plates be partially used? FAQ ID -2319 View For what type of sample material is the QIAsymphony PAXgene Blood ccfDNA Kit intended? FAQ ID - 3652 View For which applications can the RNA isolated with the RNeasy Plus Universal Kit also be used? FAQ ID -2339 View Has anyone verified whole genome amplification accuracy with Sequencing? FAQ ID -701 View Has QIAGEN ever tested Proteinase K treatment for a time period longer than overnight? View Has RNAi been successful using siRNA in Zebrafish and Xenopus? FAQ ID -400 View Have primer sequences in the Investigator IDplex GO! Kit been changed compared to the Investigator IDplex Plus assay? FAQ ID -3092 View Have QuantiTect Primer Assays been tested with QuantiFast SYBR Green PCR Kits on the Mastercycler ep realplex? FAQ ID -1714 View Have you observed co-amplification of genomic DNA from RNA templates used in the QuantiTect Whole Transcriptome Procedure? FAQ ID -1619 View Have you tested the effect of inhibitors on PCR performance? FAQ ID -818 View How accurate and reliable is PyroMark Q24 in mutation analysis? FAQ ID -2094 View How are NeXtal deep-well blocks sealed? Can they be resealed? FAQ ID -1128 View How are plasma membrane containing vesicles eluted off the ligand in the Qproteome Plasma Membrane Protein procedure? FAQ ID -1203 View How are siRNAs introduced into C. elegans? FAQ ID -396 View How are the different mutations for a single gene normalized on the qBiomarker Somatic Mutation PCR array? FAQ ID -2428 View How are the genes in each iPSC qBiomarker PCR Array selected? FAQ ID -2439 View How are the primers in the EpiTect Methyl qPCR Array designed? FAQ ID -2735 View How are the PyroMark CpG Assays reconstituted? FAQ ID -2817 View How are the PyroMark CpG Assays shipped and stored? FAQ ID -2816 View How are the supernatants resulting from the first two spin steps of the PAXgene Bone Marrow RNA kit purification procedure disposed? FAQ ID -2516 View How are the SureSilencing shRNA Plasmids shipped? FAQ ID -2884 View How best to handle the light sensitive LiquiChip reagents? FAQ ID -735 View How can a C-terminal 6xHis-tagged QIAgene be expressed? FAQ ID -2041 View How can a clogged capillary channel be identified when running samples on the QIAxcel System or QIAxcel Advanced? FAQ ID -1836 View How can a positive threshold be set when running the QIAxcel System? FAQ ID -1843 View How can air bubbles in PhosphoProtein Purification Columns be avoided? FAQ ID -1070 View How can foaming of Buffer RLT Plus lysates be avoided when using RNeasy Plus Micro and Mini Kits, or the Allprep DNA/RNA Mini Kit? FAQ ID -1734 View How can full-length genes or individual domains of the QIAgenes Insect/Mammalia constructs be subcloned? FAQ ID -2051 View How can I adjust the ROX concentration in the QuantiFast Probe RT-PCR Plus master mix? FAQ ID -2356 View How can I analyze proteins from exosomes and other extracellular vesicles (EVs) obtained with the exoEasy Maxi Kit by SDS-PAGE? FAQ ID - 3707 View How can I avoid little or no RNA yields when using an RNeasy Kit? FAQ ID -28 View How can I avoid or remove genomic DNA contamination from the total RNA preparation? FAQ ID -2662 View How can I avoid poor immunolocalization morphology with Anti-His Antibodies? FAQ ID -200 View How can I avoid primer-dimer formation during PCR amplification? FAQ ID -544 View How can I avoid variable transfection efficiencies with SuperFect Reagent? FAQ ID -179 View How can I be sure that I am harvesting my induced bacterial culture at the best time point for protein expression? FAQ ID -788 View How can I be sure that the restriction enzyme digestion is complete in the EpiTect Methyl qPCR system? FAQ ID -2731 View How can I calculate the number of bacterial cells that are present in a sample using the Microbial DNA qPCR Assays? FAQ ID — 3411 View How can I check DNA sample quality before using the Somatic Mutation PCR Arrays & Assays? FAQ ID -2430 View How can I check for purity of RNA isolated using RNeasy Kits? FAQ ID -1023 View How can I check if any residual proteins remain on the Ni-NTA Agarose matrix after elution? FAQ ID -324 View How can I check the integrity of RNA purified using RNeasy Kits? FAQ ID -1024 View How can I check the quality or size of my extracted circulating cell-free DNA? FAQ ID - 3705 View How can I check the success of the normalization procedure for the SeqTarget System? FAQ ID -2245 View How can I create a report/logfile on my QIAsymphony SP system? FAQ ID - 3594 View How can I decontaminate my QIAsymphony SP / AS system? FAQ ID - 3714 View How can I decontaminate the BioRobot EZ1? FAQ ID -1231 View How can I decontaminate the QIAcube Connect MDx system? 148739 View How can I decontaminate the QIAcube Connect system? FAQ ID - 141500 View How can I decontaminate the QIAcube system? FAQ ID -1419 View How can I decrease ribosomal RNA (rRNA) contamination using Oligotex? FAQ ID -642 View How can I desalt and concentrate a protein fraction by trichloroacetic acid (TCA) precipitation? FAQ ID -2954 View How can I determine if a tube is suitable for clot detection on QIAsymphony? FAQ ID - 3590 View How can I determine the quality of my REPLI-g amplified products? FAQ ID -695 View How can I determine whether amplification occurs from mRNA-derived cDNA or from genomic DNA contamination? FAQ ID -2687 View How can I eliminate contaminating protein in my Ni-NTA 6xHis-tag protein purification? FAQ ID -102 View How can I ensure complete genomic DNA removal when using the RNase-Free DNase Set? FAQ ID -1087 View How can I ensure optimal performance of my QIAGEN instrument? FAQ ID -1329 View How can I ensure that reaction volume is not lost due to evaporation during thermal cycling? FAQ ID -2679 View How can I estimate the size of my protein based on the length of the respective cDNA? FAQ ID -2952 View How can I express toxic protein in E. coli? FAQ ID -373 View How can I extract DNA from a polyacrylamide (PAGE) gel? FAQ ID -120 View How can I extract proteins from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue? FAQ ID -3049 View How can I find out if my gene of interest is expressed in a specific tissue type or cell line? FAQ ID - 3214 View How can I find the run parameters for a BioSprint protocol that I am running? FAQ ID -3069 View How can I get a certificate for quality assurance (ISO 13485 and ISO 9001) from QIAGEN? FAQ ID -762 View How can I get software updates for the QIAcube Connect MDx? 148734 View How can I get software updates for the QIAcube Connect? FAQ ID - 141494 View How can I get software updates for the QIAcube? FAQ ID -1413 View How can I get specific information and a quote for my individual Sequencing Service needs? FAQ ID -792 View How can I identify the binding sites of my transcription factor using ChIP? FAQ ID -2753 View How can I improve DNA yields from very tough tissues using the DNeasy Blood & Tissue Kit or the QIAamp DNA Mini Kit? FAQ ID -374 View How can I improve ligation efficiency of DNA from a QIAEX II Gel Extraction Kit? FAQ ID -141 View How can I improve my RNA yield from liver sample when using RNeasy Kits? FAQ ID -623 View How can I improve recoveries when using the QIAquick Kits? FAQ ID -180 View How can I improve the expression of proteins containing hydrophobic regions? FAQ ID -339 View How can I improve the performance of the HiSpeed QIAprecipitator module? FAQ ID -144 View How can I improve transfection efficiency using Effectene Transfection Reagent? FAQ ID -181 View How can I increase DNA concentration using QIAprecipitators of the HiSpeed Plasmid Kits? FAQ ID -1061 View How can I increase expression of my 6xHis-tagged protein in E. coli? FAQ ID -63 View How can I increase low-copy plasmid DNA yields using QIAfilter Plasmid Kits? FAQ ID -1062 View How can I increase the amount of soluble recombinant protein in E. coli expression? FAQ ID -64 View How can I increase the DNA yield from low-copy plasmids using the QuickLyse Miniprep Kit? FAQ ID -1354 View How can I isolate RNA from 1 gram of plant tissue? FAQ ID -128 View How can I keep my centrifuge tubes endotoxin-free? FAQ ID -301 View How can I know whether there is any genomic DNA present in my purified plasmid? FAQ ID -3134 View How can I load new protocols onto the QIAcube Connect MDx? 148740 View How can I load new protocols onto the QIAcube Connect? FAQ ID - 141501 View How can I load new protocols onto the QIAcube? FAQ ID -1421 View How can I minimize degradation of RNA from my pancreas sample? FAQ ID -624 View How can I normalize my EpiTect ChIP results? FAQ ID -2751 View How can I obtain DNA-free RNA using an RNeasy Midi or Maxi Kit? FAQ ID -143 View How can I optimize Mg2+ conditions for a specific amplicon when using Phoenix Hot Start Taq and the supplied reaction buffer? FAQ ID - 3902 View How can I optimize Mg2+ conditions for a specific amplicon when using VeraSeq 2.0 DNA Polymerase and the supplied reaction buffers? FAQ ID - 3909 View How can I optimize Mg2+ conditions for a specific amplicon when using VeraSeq ULtra DNA Polymerase and the supplied reaction buffers? FAQ ID - 3922 View How can I optimize the transfection of oligos and large plasmids using PolyFect Transfection Reagent? FAQ ID -176 View How can I precipitate genomic DNA using isopropanol? FAQ ID -2953 View How can I predict the percent qPCR signal due to contaminating DNA, for a given qPCR assay, and its matching NRT control? FAQ ID -2688 View How can I prevent clogging of QIAfilter cartridges? FAQ ID -1060 View How can I prevent non-specific binding when using BioMag? FAQ ID -277 View How can I prevent running out of M48 Kit reagents before the full number of samples are processed? FAQ ID -1042 View How can I prevent the evaporation of reaction volume from the wells EpiTect Methyl qPCR Arrays? FAQ ID -2742 View How can I prevent the evaporation of reaction volume from the wells in the miScript miRNA PCR array? FAQ ID -2729 View How can I purify DNA from soil, food and sewage samples for PCR? FAQ ID -118 View How can I purify mRNA from total RNA using an Oligotex Direct mRNA Kit? FAQ ID -201 View How can I purify very small amounts of 6xHis-tagged protein using Ni-NTA technology? FAQ ID -134 View How can I quantify the amount of REPLI-g DNA I have amplified? FAQ ID -694 View How can I remove imidazole from a protein sample? FAQ ID -91 View How can I resuspend the lyophilized assay? FAQ ID - 3864 View How can I separate PCR fragments that are small and very close in size on an agarose gel? FAQ ID -800 View How can I streamline the quantification workflow? FAQ ID - 3738 View How can I tell if I have primer-dimers in my PCR reaction? FAQ ID -552 View How can I tell TurboCapture Microplate Holder Type A from Type B? FAQ ID -1039 View How can I transfect siRNA into insect cells such as Drosophila melanogaster S2? FAQ ID -1046 View How can I upload my gene list for FlexiPlate siRNA to GeneGlobe? FAQ ID -1364 View How can I use the SureSilencing shRNA Plasmids for stable transfection and knock down in a cell line that is already resistant to both neomycin and puromycin? FAQ ID -2895 View How can one determine the optimal annealing temperature for a specific PCR assay? FAQ ID -288 View How can one perform multiplex real-time PCR analysis using the LightCycler 2.0? FAQ ID -780 View How can PCR cycling conditions be optimized for Phoenix Hot Start Taq? FAQ ID - 3899 View How can proteins purified from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue be analyzed? FAQ ID -3052 View How can QIAGEN Protease and Proteinase K be inactivated? FAQ ID -315 View How can RNA interference be used as a life science research tool? FAQ ID -2756 View How can samples be shipped and stored prior to extraction on the Autopure LS? FAQ ID - 3522 View How can samples be shipped and stored prior to extraction on the Autopure LS? FAQ ID - 3528 View How can the QIAsymphony SP system be decontaminated? FAQ ID -1914 View How can we get our BioRobot EZ1 upgraded when new protocols are launched? FAQ ID -1241 View How can yield for long targets be increased when using Phoenix Hot Start Taq? FAQ ID - 3907 View How can yield for targets be increased when using VeraSeq 2.0 DNA Polymerase? FAQ ID - 3914 View How can yield for targets be increased when using VeraSeq ULtra DNA Polymerase? FAQ ID - 3926 View How comparable is CoralLoad gel loading dye contained in various QIAGEN PCR Kits to Sigma Red? FAQ ID -1644 View How complete is the removal of DAPase in the TAGZyme process? FAQ ID -319 View How critical is the time of the temperature change from 60°C to 80°C? FAQ ID - 143768 View How did you calculate the conversion factor? FAQ ID - 3937 View How did you determine that your AllStar Negative Control siRNA binds to RISC? FAQ ID -1180 View How do I analyze codon 61 mutations with the RAS extension kit (cat. no. 971590)? FAQ ID - 3622 View How do I analyze the data for the particular iPSC qBiomarker Arrays I have ordered? FAQ ID -2440 View How do I calculate the percentage of silencing with real-time RT-PCR for siRNA? FAQ ID -498 View How do I choose between the DNA-based Cignal Reporter Assays and the Cignal Lenti Reporter Assays? FAQ ID -2761 View How do I choose EpiTect ChIP-grade antibodies? FAQ ID -2746 View How do I clean up RNA preparations containing miRNA? FAQ ID -3002 View How do I create a workspace that is free of DNA contamination, prior to carrying out a qPCR experiment? FAQ ID -2654 View How do I determine if my gene expression results are statistically significant in the REST software? FAQ ID -2458 View How do I determine the amplification efficiency of my qPCR assay? FAQ ID -2694 View How do I determine the linear dynamic range of my qPCR or qRT-PCR assay? FAQ ID -2696 View How do I find the SureSilencing shRNA Plasmids for my gene of interest? FAQ ID -2883 View How do I get started using the NeXtal CubicPhase kits? FAQ ID -2413 View How do I harvest the crystals from meso phase? Are special tools needed with the NexTal Cubic Phase products? FAQ ID -2330 View How do I harvest the crystals from meso phase? Are special tools needed? FAQ ID -2422 View How do I insert and replace an absorber strip into the PyroMark Q48 Autoprep chamber? FAQ ID - 3691 View How do I know if my plasmid is a high- or low copy number type? FAQ ID -350 View How do I know if the assays are compatible with the sample DNA of interest? FAQ ID - 3870 View How do I know if the CGT dPCR assays are compatible with the sample DNA of interest? FAQ ID - 3980 View How do I know in which phase of my NeXtal CubicPhase cristallisation experiment I am? FAQ ID -2421 View How do I know whether my miRNA has been successfully purified and how much is expected? FAQ ID -3135 View How do I obtain the concentration of my gDNA from the data analysis using the GeneRead DNA QuantiMIZE? FAQ ID - 3530 View How do I perform a DNA precipitation to concentrate my sample? FAQ ID -305 View How do I perform an Acetone Precipitation for concentrating and desalting protein samples? FAQ ID -1035 View How do I perform the PyroMark Q24 Advanced upgrade? FAQ ID -3163 View How do I prepare an insert for pQE vectors? FAQ ID -185 View How do I prepare Buffer MP? FAQ ID - 3620 View How do I prevent a drifting baseline in my pyrosequencing pyrogram? If this method cannot be selected automatically in the application software, you can download the method/code file from the instrument webpage. FAQ ID -2878 View How do I prevent bubbles from forming in my Ni-NTA agarose column? FAQ ID -285 View How do I proceed if the amount of starting material cannot be calculated in detail? FAQ ID - 3943 View How do I reduce background peaks in the pyrosequencing pyrogram? FAQ ID -2877 View How do I remove the RNA contamination from purified DNA? FAQ ID -3133 View How do I resuspend my FlexiPlate siRNAs? FAQ ID -1360 View How do I safely inactivate biohazardous flow-through material? FAQ ID -12 View How do I search for all the miScript Target Protectors for one species? FAQ ID -2255 View How do I set up a PyroMark CpG Assay? FAQ ID -2814 View How do I store my FlexiTube siRNA Premix? FAQ ID -2270 View How do I submit a siRNA order by telephone or online? FAQ ID -399 View How do I use EasyXtal Crystallization Tools? FAQ ID -1105 View How do I use the qBiomarker Somatic Mutation PCR Arrays? FAQ ID -2923 View How do QuantiFast PCR Kits compare to QuantiTect PCR Kits for quantitative real-time PCR? FAQ ID -1441 View How do Rotor-Gene Probe Kits compare with QuantiFast Probe Kits? FAQ ID -2125 View How do the FastLane Cell cDNA Kit and QuantiTect Reverse Transcription Kit eliminate genomic DNA contamination? FAQ ID -783 View How do Unique Molecular Indexes (UMIs) improve quantification? FAQ ID - 3669 View How do you achieve fast cycling, yet still deliver the same performance in PCR as that achieved with standard cycling? FAQ ID -1430 View How do you choose which assays to include on the arrays? FAQ ID — 3420 View How do you decide on the qBiomarker Somatic Mutation PCR Arrays to include on the pathway arrays and disease arrays? FAQ ID -2913 View How do you design your EpiTect ChIP qPCR Assay primers? Do they work with SB GR? FAQ ID -2749 View How do you determine the efficiency using the PCR array? FAQ ID -2701 View How do you ensure that concentrated RNA is purified from saliva using the RNeasy Protect Saliva Mini Kit? FAQ ID -1212 View How do you ensure that RNeasy buffers are RNase-free? FAQ ID -113 View How does a Service Support Agreement provide defined budgeting for maintaining my BioRobot? FAQ ID -1330 View How does HotStart PCR help minimize nonspecific amplification events? FAQ ID -2676 View How does imidazole affect my quantitation of protein? FAQ ID -132 View How does magnetic-particle carry-over in QIAsymphony eluates need to be handled? FAQ ID -2166 View How does QIAGEN control the quality of the RT² qPCR Primer Assays? FAQ ID -2711 View How does QIAseq FastSelect work? FAQ ID -147907 View How does QIAsymphony SP keep cellular samples in suspension prior to pipetting so that samples do not settle? FAQ ID -1905 View How does stable transfection of a shRNA-encoding plasmid work? FAQ ID -2779 View How does template quality influence the results with the Type-it HRM PCR Kit? FAQ ID -2197 View How does the built-in QC control for complete bisulfite conversion of DNA work on PyroMark Q24? FAQ ID -2095 View How does the DNA Protection work in the EpitTect Bisulfite kits? FAQ ID -2410 View How does the gDNA eliminator solution of the RNeasy Plus Universal Kit work? FAQ ID -2341 View How does the liquid level detection work and what is the minimal detectable volume for the conductive tips of the BioRobot Universal? FAQ ID - 3524 View How does the QuantiFast Probe RT-PCR Plus Kit eliminate genomic DNA contamination? FAQ ID -2354 View How does the RNeasy Microarray Tissue Mini Kit prevent traces of phenol from negatively affecting the reverse-transcription step in microarray experiments? FAQ ID -2189 View How does the sealing efficiency of the crystallization supports of the EasyXtal 15-Well Tool compare to grease? FAQ ID -1109 View How does the stabilizing reagent in the PAXgene Saliva Collector stabilize saliva? 3814 View How easy is it to scoop up a protein crystal with the lip around the crystallization supports of the EasyXtal 15-Well Tools? FAQ ID -1113 View How efficient is DNA removal by a gDNA Eliminator 96 plate compared with DNase digestion on an RNeasy 96 plate? FAQ ID -1994 View How fast does the rotor spin during a run on the Rotor-Gene Q? FAQ ID -2080 View How fast is the 6xHis-tagged protein purification process using Ni-NTA Superflow Cartridges? FAQ ID -1605 View How great is the enrichment of miRNA when using the dedicated PAXgene Blood miRNA Kit compared to the PAXgene Blood RNA Kit? FAQ ID -2495 View How important is the RNA purification process, for obtaining reliable qRT-PCR results? FAQ ID -2655 View How is "Touchdown PCR" used to increase PCR specificity? FAQ ID -75 View How is an antibody epitope determined? FAQ ID -593 View How is ARMS technology superior to alternative methods such as dye terminator sequencing? FAQ ID -2290 View How is stability and performance of these mericon Assay kits guaranteed? FAQ ID -2542 View How is the hypothesis test performed in the REST software? FAQ ID -2459 View How is the log graph threshold value (Ct) for the Rotor-Gene Q determined? FAQ ID -2088 View How is the Pyromark Q48 cartridge to be cleaned? FAQ ID - 3718 View How is the quality of proteins purified from PAXgene Tissue fixed and stabilized tissue? FAQ ID - 3619 View How is the quality of the crystallization condition Screening Suites guaranteed? FAQ ID -1126 View How is the RNeasy Lipid Tissue Mini Kit different from the RNeasy Mini Kit? FAQ ID -468 View How is the RNeasy Microarray Tissue Mini Kit different from the classic RNeasy Mini Kit? FAQ ID -2192 View How is the RNeasy Plus Universal Kit different from the RNeasy Plus Kit? FAQ ID -2340 View How is total RNA purified with RNeasy kits free of genomic DNA contamination? FAQ ID -3154 View How is VeraSeq 2.0 DNA Polymerase different from the standard recombinant Taq-B DNA Polymerase? FAQ ID - 3908 View How is VeraSeq ULtra DNA Polymerase different from the standard recombinant Taq-B DNA Polymerase? FAQ ID - 3921 View How long after first use can a reagent cartridge be stored for further use? FAQ ID - 3650 View How long and at which temperature can plasma generated from whole blood in PAXgene Blood ccfDNA Tubes be stored? FAQ ID - 3644 View How long are sample lysates stable in the fridge/ freezer? FAQ ID - 143765 View How long can a reagent cartridge remain open without affecting my results? FAQ ID - 3706 View How long can a tissue specimen stay in the PAXgene Tissue STABILIZER? FAQ ID -3028 View How long can DNA converted with EpiTect Bisulfite Kits be stored before use in methylation specific PCR with the EpiTect MSP Kit? FAQ ID -2002 View How long can eluates be stored after sample preparation using the EZ1 instruments or EZ2 Connect MDx and the EZ1 DSP Virus Kit? FAQ ID - 3897 View How long can homogenates remain on the QIAsymphony SP without risk of RNA degradation? FAQ ID -1941 View How long can I archive cDNA generated with the QuantiTect Whole Transcriptome Kit? FAQ ID -1623 View How long can I store an RLT lysate? FAQ ID -3115 View How long can I store bacterial culture stabilized in RNAprotect Bacteria Reagent? FAQ ID -614 View How long can I store my purified DNA/RNA isolated with the AllPrep DNA/RNA FFPE kit? FAQ ID -2350 View How long can I store the dilutions of the Control DNA Z1 standards at 2°C? -8 View How long can I store the dilutions of the Male Control DNA M1 standards at 2–8°C? FAQ ID - 3744 View How long can I store the reconsituted assay regarding QIAcuity HEK293 resDNA Quant Kit (96) at 4°C? FAQ ID - 3934 View How long can I store the reconstituted Internal Control and the reconstituted Positive Control? FAQ ID - 3997 View How long can primer and probes from the mericon Assay kits be kept at°C once reconstituted? -20 View How long can reaction components incubate with VeraSeq 2.0 DNA Polymerase at room temperature prior to PCR cycling? FAQ ID - 3917 View How long can saliva in PAXgene Saliva Collector tubes be stored at room temperature? 3815 View How long can samples in PAXgene Blood DNA Tubes be stored at room temperature? FAQ ID - 3508 View How long can the PAXgene Blood DNA Tube be kept at room temperature? What is room temperature? FAQ ID - 3492 View How long can the PAXgene Blood RNA tubes be kept frozen at –20°C or –70°C? FAQ ID - 3463 View How long can whole blood stabilized in PAXgene Blood ccfDNA Tubes be stored at room temperature? FAQ ID - 3639 View How long does a QuantiFast Pathogen + IC run take on the Rotor-Gene Q? FAQ ID -2452 View How long does a run take? FAQ ID -2566 View How long does a saliva collection take? 3804 View How long does a single run on the PyroMark Q24 take? FAQ ID -2096 View How long does a typical in vitro translation reaction take with your large-scale EasyXpress protein synthesis kits? FAQ ID -880 View How long does it take to generate results using the KRAS and EGFR PCR kits? FAQ ID -2299 View How long does it take to set up a 96-well plate PCR reaction on the QIAgility? FAQ ID -3066 View How long does the sample disruption with the TissueLyser LT take? FAQ ID -2550 View How long is cDNA generated with the QuantiTect Whole Transcriptome Kit? FAQ ID -1613 View How long is fluorescence detectable in cells after transfection with fluorescently labeled siRNAs? FAQ ID -392 View How long is the fixation time when using PAXgene Tissue FIX Container? FAQ ID -3026 View How long is the fixation time? FAQ ID -2519 View How long should I wait after transfection before enriching my transfected cells, validating knock down, and looking for phenotypic changes? FAQ ID -2893 View How long should PCR fragments be for the SeqTarget System? FAQ ID -2236 View How long should the amplicon be if I am using QuantiTect SYBR Green detection? FAQ ID -553 View How long will the intensity calibration procedure on the QIAxcel System take? FAQ ID -1826 View How magnetically responsive is BioMag? FAQ ID -265 View How many AAV genome copies can I load into the QIAcuity? FAQ ID - 3977 View How many assays can be multiplexed? FAQ ID - 3862 View How many cells can be used with the FastLane Cell cDNA Kit? FAQ ID -796 View How many cells can be used with the Qproteome Mitochondria Isolation Kit, and how much mitochondrial protein can be expected? FAQ ID -1189 View How many CpG sites are analyzed by the PyroMark CpG LINE assay? 2858 View How many different genes and gene mutations are on a Somatic Mutation PCR Array? FAQ ID -2427 View How many drops can be set up on a protein crystallization support? FAQ ID -1110 View How many elution steps are recommended when using Glutathione Superflow resin? FAQ ID -2175 View How many experiments can I do with the Cignal Lenti Reporter Assays? FAQ ID -2769 View How many extractions can I perform with a QIAsymphony PAXgene Blood ccfDNA Kit, using the 2.4 ml or the 4.8 ml protocols? FAQ ID - 3649 View How many freeze-thaw cycles can the Internal Control supplied with Certal Residual DNA Detection Kits tolerate? FAQ ID -2975 View How many freeze-thaw cycles can the master mix contained in the QuantiFast Probe RT-PCR Plus Kit tolerate? FAQ ID -2357 View How many freeze-thaw cycles can the various reaction mixes in the EasyXpress Insect Kit II tolerate? FAQ ID -1219 View How many housekeeping genes are included in each PCR Array? FAQ ID -2704 View How many LiquiChip beads are recommended for an xMAP experiment? FAQ ID -1161 View How many miScript Precursor Assays do you offer? FAQ ID -2213 View How many molecules can be detected in the system? 153926 View How many mutations can I profile per sample on the qBiomarker Somatic Mutation PCR Array? FAQ ID -2911 View How many nucleotides of a homopolymer can be resolved in pyrosequencing? FAQ ID -2871 View How many PAXgene Blood RNA Tubes can be processed per PAXgene 96 Blood RNA Kit well plate? FAQ ID -2485 View How many PCR reactions can be performed for different scales of miScript Primer Assays? FAQ ID -1990 View How many protein crystallization conditions are available at QIAGEN? FAQ ID -1119 View How many reactions can be performed per well of the QuantiTect Primer Assay Plates? FAQ ID -2107 View How many reactions can be performed with one QIAsymphony mericon Bacteria Kit? FAQ ID -2955 View How many reactions can I perform with the new QuantiFast Kits for real-time PCR? FAQ ID -1425 View How many reporter dyes can be used in the same analysis using the LiquiChip Workstation? FAQ ID -738 View How many samples can be processed per kit or per reagent cartridge for RNA purification on the QIAsymphony SP? FAQ ID -1940 View How many samples can be processed per RNeasy Mini QIAcube kit? FAQ ID -2332 View How many samples can be processed with the PAXgene Blood miRNA Kit? FAQ ID - 3477 View How many samples can be processed with the PAXgene Blood RNA MDx Kit? FAQ ID -2490 View How many samples can be processed with the QIAsymphony DNA Mini Kit and the Virus Blood 200 protocol? FAQ ID -2159 View How many samples can be processed with the QIAsymphony DSP Virus/Pathogen Kits? FAQ ID -2935 View How many samples can be processed with the QIAsymphony PAXgene Blood RNA Kit? FAQ ID -2497 View How many samples can be run in parallel with the Rotor-Gene Q real-time PCR instrument? FAQ ID -1519 View How many signal readings are taken during the real-time data acquisition on the Rotor-Gene Q? FAQ ID -2083 View How many template copies can I detect using QuantiTect Kits in real-time PCR? FAQ ID -543 View How many times can the cartridges for PyroMark Q24 or PyroMark Q96 ID instruments be reused? FAQ ID -2863 View How many times can the CDTs, NDTs, and RDTs be used? FAQ ID -2865 View How many times can the Internal Controls used in the QuantiFast Pathogen PCR +IC Kit and the QuantiFast Pathogen RT-PCR +IC Kit be freeze-thawed? FAQ ID -2599 View How many times can vacuum troughs be reused with the PyroMark Vacuum Preparation Stations? FAQ ID -2848 View How many transfections can be performed with Flexiplate siRNAs? FAQ ID -3105 View How many transfections can I perform with 20 nmol of HPP grade siRNA? FAQ ID -367 View How much active protein can be expressed in-vitro using the EasyXpress Insect Kit II, and how much time does it take? FAQ ID -1222 View How much buffer should be used for agarose gel electrophoresis? FAQ ID -2257 View How much can the mixed standard ?Ct values vary in the KRAS PCR kit? FAQ ID -2307 View How much cDNA is sufficient for real-time PCR detection of miRNA transcripts generated with the miScript System? FAQ ID -1598 View How much circulating cell-free DNA can I expect from samples, and how can I quantify the yield? FAQ ID - 3704 View How much detection reagent do you generally recommend for LiquiChip Assays? FAQ ID -1159 View How much DNA and RNA can be expected from human blood cells? FAQ ID -2950 View How much DNA does a bacterial cell contain? FAQ ID -2947 View How much DNA does a human cell contain? FAQ ID -2948 View How much DNA is needed in the PyroMark Q48? FAQ ID - 3721 View How much DNA is obtained in the average PCR reaction? FAQ ID -750 View How much DNA should be loaded per well of an agarose gel? FAQ ID -2259 View How much DNA should I use in a qBiomarker Copy Number PCR Assay? FAQ ID — 3418 View How much DNA should I use on a qBiomarker Copy Number PCR Array? FAQ ID — 3417 View How much DNA starting sample is needed for analysis with a Somatic Mutations PCR Array? And Assay? FAQ ID -2431 View How much DNA/RNA can be isolated with the AllPrep DNA/RNA FFPE Kit? FAQ ID -2353 View How much dye-labeled siRNA should I use to monitor transfection efficiency when using HiPerfect Transfection Reagent? FAQ ID -1058 View How much protein can be produced with the EasyXpress Protein Synthesis Mega Kit? FAQ ID -843 View How much protein does one vial of Protease in the QIAamp spin kits contain? What is the concentration of the resuspended Protease? FAQ ID - 3520 View How much REPLI-g amplified mitochondrial DNA should be used in a subsequent PCR reaction? FAQ ID -1320 View How much RNA does a bacterial cell contain? FAQ ID -2949 View How much RNA does a typical mammalian cell contain? FAQ ID -2946 View How much sample can be used with the Allprep DNA/RNA 96 Kit? FAQ ID -1997 View How much sample can be used with the RNeasy Plus 96 Kit? FAQ ID -1992 View How much sample can I load for detecting host cell contamination? FAQ ID - 3879 View How much sample is required for each well for an ELISArray Kit? FAQ ID -2908 View How much sample material can I use for DNA/RNA purification with the AllPrep DNA/RNA FFPE Kit? FAQ ID -2351 View How much solution can I add to an EasyXtal 15-Well Tool reservoir well? FAQ ID -1114 View How much space does the PyroMark Q24 instrument need? FAQ ID -2098 View How much space does the PyroMark Q48 Autoprep instrument need? FAQ ID - 3693 View How much starting material can be used with the QIAsymphony DNA Mini Kit on the QIAsymphony SP? FAQ ID -1917 View How much starting material can be used with the QIAsymphony DSP DNA Mini Kit on the QIAsymphony SP? FAQ ID -2927 View How much template can I use in the reaction and what is the maximum volume of template that can be used in the QuantiTect Kits? FAQ ID -1086 View How much time can be saved when using the RNeasy Mini QIAcube Kit? FAQ ID -2335 View How much time is needed for a LiquiChip assay point measurement? FAQ ID -1162 View How much time may the aspirated bone marrow sample be allowed to incubate before pipetting it into the PAXgene Bone Marrow RNA Tube? FAQ ID -2504 View How much time will be saved when switching from standard cycling to fast cycling with QuantiFast Kits? FAQ ID -1438 View How often can the Strep-Tactin Superflow Plus resin be reused? FAQ ID -2169 View How often should the alignment marker for the QIAxcel System be changed? FAQ ID -1839 View How often should the o-ring for the pipettor tip adapter be changed on the QIAcube Connect? FAQ ID - 141509 View How often should the solutions in the Buffer Tray for the QIAxcel System or QIAxcel Advanced be replaced? FAQ ID -1840 View How precise is the QIAgility? FAQ ID -3063 View How pure are the CTCs enriched from blood samples using the AdnaTest? 3831 View How pure should my protein be with the NexTal CubicPhase kits? FAQ ID -2414 View How pure should the chloroform used with the RNeasy Microarray Tissue Mini Kit be? FAQ ID -2193 View How pure should the chloroform used with the RNeasy Plus Universal Mini Kit be? FAQ ID -2344 View How reliable are the results from the miScript Primer Assays? FAQ ID -2730 View How sensitive is the LiquiChip Reader? FAQ ID -1157 View How should blood samples drawn into PAXgene Blood DNA Tubes be shipped? FAQ ID - 3489 View How should blood samples drawn into PAXgene Blood DNA Tubes be transported? FAQ ID - 3507 View How should blood samples drawn into the PAXgene Blood DNA Tubes be stored? FAQ ID - 3490 View How should data be analyzed when the miScript Single Cell qPCR Kit is used with miScript miRNA PCR Arrays or miScript Primer Assays? FAQ ID - 3581 View How should DNA isolated on the QIAsymphony be stored? FAQ ID -2165 View How should DNA purified using the PAXgene Blood DNA System be stored? FAQ ID - 3502 View How should eluates produced with the PAXgene Blood ccfDNA System be stored? FAQ ID - 3657 View How should fluorescent labeled probes be stored? FAQ ID -784 View How should gels be cast with the GelPilot Agarose so that optimal resolution is achieved? FAQ ID -2258 View How should I analyze the data generated from a qBiomarker Copy Number PCR Array experiment? FAQ ID — 3421 View How should I dissolve the lyophilized Anti-His and Tag Antibodies? -100 View How should I prepare buffy coat samples for use on the BioRobot EZ1? FAQ ID -1249 View How should I propagate pQE expression plasmids? FAQ ID -58 View How should I quantify RNA isolated with RNeasy Kits? FAQ ID -32 View How should I reconstitute the lyophilized RNase-Free DNase Set with the RNeasy 96 kit? FAQ ID -3003 View How should I store Anti·His and Tag·100 Antibodies? FAQ ID -194 View How should I store cDNA produced using Omniscript Reverse Transcriptase? FAQ ID -561 View How should I store plant material for DNA isolation using the DNeasy Plant Kit? FAQ ID -114 View How should I store the REPLI-g amplification product? FAQ ID -692 View How should I thaw frozen PAXgene Blood DNA Tubes? FAQ ID - 3495 View How should I thaw frozen plasma samples? FAQ ID - 3645 View How should QIAGEN Plasmid Purification Kits be stored and for how long? FAQ ID -192 View How should RNeasy Kits be stored and how long are they stable? FAQ ID -103 View How should samples be stored before purification using the Virus Blood 200 Protocol on the QIAsymphony? FAQ ID -2157 View How should the GelPilot DNA Loading Dye be handled? FAQ ID -1287 View How should the PAXgene Bone Marrow RNA Tubes be frozen after collection and for how long? FAQ ID -2505 View How should the samples be stored before purification of RNA with the QIAsymphony PAXgene Blood RNA Protocol? FAQ ID -2984 View How should the samples be stored before purification with the QIAsymphony DSP Virus/Pathogen Kits? FAQ ID -2934 View How should unused PAXgene Blood ccfDNA Tubes be stored? FAQ ID - 3634 View How should unused PAXgene Saliva Collector be stored? 3812 View How stable is Phoenix Hot Start Taq when incubated in a PCR reaction mix at room temperature? FAQ ID - 3898 View How to prepare DNA prior to dPCR? 3778 View How to quantitate DNA purified using the BioSprint 96? FAQ ID -773 View How to store DNA from forensic samples eluted into water on the EZ1 biorobot in TE buffer instead? FAQ ID -734 View How to upload a Flexiplate on QIAGEN Webpage? FAQ ID -3103 View How was it determined that the PhosphoSerine Antibody will bind phosphoserines only? FAQ ID -579 View How well is DNA integrity preserved in PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue? FAQ ID - 3612 View How well is RNA integrity preserved in PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue? FAQ ID - 3611 View How will running less than 96 samples with the MagAttract 96 cador Pathogen kit affect usage of plasticware? FAQ ID -2071 View How will service work carried out on my QIAGEN BioRobot be documented? FAQ ID -1331 View How would I provide information on the QuantiTect Primer assays in publications when the sequence is confidential? FAQ ID - 3593 View I accidentally added RNAprotect Tissue Reagent to my cells, and now the cells are difficult to pellet. What can I do? FAQ ID -364 View I accidentally stored Buffer RDD of the RNase-Free DNase Set at°C. Will it still function? -20 View I am seeing a precipitate after adding LyseBlue reagent to Buffer P1. What should I do about that? FAQ ID -1045 View I am using an RNA library prep kit that is not listed in the QIAseq FastSelect handbook. Will QIAseq FastSelect work with my kit? FAQ ID -147913 View I bound an 11 kb DNA fragment to a QIAquick column; is it completely lost? FAQ ID -756 View I cannot analyze the data from your Investigator HID kits with my GeneMapper software. What can I do? FAQ ID - 3363 View I cannot see the LCD display fonts on the Sigma Centrifuge. How do I adjust the contrast? FAQ ID -822 View I do not see any miRNA expression for my miRNAs of interest after performing single cell miRNA expression analysis with the miScript Single Cell qPCR Kit and miScript miRNA PCR Arrays or miScript Primer Assays. What could be the cause? FAQ ID - 3577 View I don’t have any glassware in the lab, is the QIAwave kit still a good option for me? FAQ ID - 3989 View I have a Human Druggable Genome siRNA Set V2.0. Can I reorder the siRNAs from this set? FAQ ID -1366 View I have accidentally pipetted beads when removing the eluate after cDNA cleanup. What should I do? FAQ ID - 3572 View I left Buffer P1 at room temperature after addition of RNase A, what shall I do? FAQ ID -859 View I need to buy a new computer for my PyroMark Q96. Can I reinstall the PyroMark Q96 software on my new computer or do I need to purchase a new license? FAQ ID - 3472 View I ran my RNA out on an agarose gel and can see lots of bands similar to a ladder. Why? FAQ ID -745 View I received a kit containing the MinElute columns; however, they were left out for a while and not stored at 2–8°C upon receipt. Can I still use them? FAQ ID - 3560 View I routinely heat an aliquot of the RNA eluate prior to my RT reaction. Do I still need to heat the eluate after the PAXgene Bone Marrow RNA preparation? FAQ ID -2513 View I use cultured cells as starting material. Can I also use the RNeasy Plus Universal Kit? FAQ ID -2342 View I used to buy BioMag particles from a company by another name? Are your BioMag particles the same thing? FAQ ID -263 View I want to purify RNA from heart tissue. What is the best kit to use? FAQ ID -3116 View I want to start with gene silencing (RNAi) experiments. Do you have informational literature? FAQ ID -580 View I would like to order the QuantiFast Probe Assays without master mix. Is this possible? FAQ ID -2360 View I would like to sequence my siRNA constructs. Does QIAGEN offer Sequencing Services for cloned siRNA? FAQ ID -829 View I would like to stop during the library preparation procedure. When can I do that? FAQ ID - 3687 View I would like to use the kit on another third party instrument. Who should I contact? 3803 View If 400 µl are used as lysis volume, how do I concentrate the lysate? (Otherwise the DNA would be too diluted.) FAQ ID - 143766 View If a cycler from Applied Biosystems is used in combination with the Certal Residual DNA Detection Kit, should dye calibration for MAX be performed? FAQ ID -2977 View If a QuantiTect Primer Assay has more than one version number, does this mean that earlier assay versions may not work? FAQ ID -1136 View If both MaXtract Low Density and High Density is suitable for an application, which type is preferable to use? FAQ ID -1310 View If FlexiPlate siRNA was accidentally redissolved in siRNA Suspension Buffer instead of water, resulting in a 2x buffer, would it affect performance of the siRNAs? FAQ ID -1477 View If housekeeping and target genes differ significantly in abundance, will they both be amplified with equal efficiency using the Rotor-Gene Multiplex PCR Kit? FAQ ID -2129 View If I am using BioMag Streptavidin to capture biotinylated oligos, how can I then remove the oligos? FAQ ID -279 View If I am working with a difficult-to-transfect cell type or if I obtain only weak silencing effects what can I do? FAQ ID -2265 View If I cannot find a QuantiTect Primer Assay for my target gene in rat, can I use the assays for the orthologous genes in mouse and human? FAQ ID -1145 View If I cannot order FlexiPlate siRNA online, how do I order? FAQ ID -1371 View If I want to use BioMag particles, do I have to buy a QIAGEN magnet too or can I use one that I already have? FAQ ID -266 View If less than 48/96 samples are being processed, is it possible to leave positions empty with the PAXgene Blood RNA MDx Kit? FAQ ID -2492 View If new dyes or chemistries appear on the real-time PCR market, will Rotor-Gene Q be capable of running those dyes? FAQ ID -2087 View If orders cannot be placed online, how can QIAgenes be ordered? FAQ ID -2046 View If pQE40 is used to express a fusion protein with DHFR, can the DHFR be cleaved afterwards? FAQ ID -513 View If template abundance differs significantly between housekeeping and target gene, are both amplified equally efficient with the QuantiTect Multiplex Kits? FAQ ID -718 View If the M500 method is used with the QIAxcel System, does a new DNA reference marker table have to be created? FAQ ID -1856 View If Vapor-Lock is used to overlay PCR reactions, which volume should be entered as reaction volume in the Rotor-Gene Q Software? FAQ ID -2150 View In the REST software, what is the range indicated by 'Std. Error' on the 'Results' page? FAQ ID -2455 View In the results table generated with the QIAxcel System, what does "normalized area" mean? FAQ ID -1854 View In which channels can you detect the CGT dPCR assays? FAQ ID - 3863 View In which format can PyroMark CpG Assays be ordered? FAQ ID -2824 View In which formats are crystallization condition Screening Suites available? FAQ ID -1127 View In which solution can I keep my protein for the NexTal Cubic Phase kits? FAQ ID -2416 View In which tubes is clot detection possible on QIAsymphony? FAQ ID - 3589 View Investigator Quantiplex and Quantiplex Pro: What do I have to consider if I am using Applied Biosystems SDS software version 1.2.3? FAQ ID -2570 View Investigator Quantiplex Pro RGQ: On which real-time cyclers is the kit validated? FAQ ID - 3741 View Investigator Quantiplex Pro RGQ: Which real-time cycler software version can be used to run the kit? FAQ ID - 3743 View Investigator Quantiplex Pro RGQ: Which Rotor-Gene Q System can be used to run the kit? FAQ ID - 3742 View Investigator Quantiplex Pro: Can the kit be run on the Rotor-Gene Q? FAQ ID - 3729 View Investigator Quantiplex Pro: Do I have to calibrate new dyes on my Applied Biosystems 7500 or QuantStudio 5 Real-Time PCR System? FAQ ID - 3732 View Investigator Quantiplex Pro: On which real-time cyclers is the Investigator Quantiplex Pro Kit validated? FAQ ID - 3728 View Investigator Quantiplex Pro: Which real-time cycler software version can be used to run the kit? FAQ ID - 3730 View Investigator Quantiplex: Do I have to calibrate new dyes on my Applied Biosystems 7500 or 7500 Fast Real-Time PCR System? FAQ ID -2571 View Investigator Quantiplex: For which real-time cyclers has the kit been validated? FAQ ID -2567 View Investigator Quantiplex: Which version of the Applied Biosystems 7500 Real-Time PCR System software can be used to run the Investigator Quantiplex? FAQ ID -2569 View Investigator Quantiplex: Which version of the Rotor Gene-Q software can be used to run the kit? FAQ ID -2568 View Is 2 kb the minimal gDNA fragment size for REPLI-g Whole Genome Amplification? FAQ ID -682 View Is a passive internal reference dye, like ROX, required on the QIAquant to obtain reproducible results? 153920 View Is a passive internal reference dye, like ROX, required on the Rotor-Gene Q to obtain reproducible results? FAQ ID -2079 View Is a restriction digest necessary for DNA isolated from PAXgene Saliva samples before performing a dPCR run using the QIAcuity? 3829 View Is a saliva collection device provided in the RNeasy Protect Saliva Mini Kit? FAQ ID -1213 View Is a special processing protocol needed? FAQ ID -2523 View Is a special processing protocol needed? FAQ ID -3035 View Is a standard curve needed in dPCR? 3786 View Is an additional proteinase K digest possible with DNeasy PowerSoil Kit and DNeasy PowerLyzer Kit? FAQ ID - 3725 View Is cDNA generated with the QuantiTect Whole Transcriptome Kit suitable for use in microarray analysis? FAQ ID -1587 View Is clot detection possible on the QIAsymphony? FAQ - ID 3588 View Is dihydrofolate reductase (DHFR) immunogenic? FAQ ID -471 View Is DNA extraction from the samples needed? FAQ ID - 3873 View Is double centrifugation required for plasma processing? FAQ ID - 3643 View Is dPCR sensitive to inhibition? FAQ ID - 3880 View Is Factor SB of the HotStar HiFidelity Polymerase Kit the same as Factor MP of the QIAGEN Multiplex PCR Kit? FAQ ID -1049 View Is FlexiTube GeneSolution available for other scales than the 1 nmol scale? FAQ ID -1666 View Is food DNA co-purified using the QIAsymphony mericon Bacteria Kit? FAQ ID -2959 View Is GFP excited and detected by the green laser of the LiquiChip System? FAQ ID -737 View Is it acceptable to correct for transfection efficiency, when determining the level of gene expression knock down by an shRNA expression vector? FAQ ID -2783 View Is it also possible to isolate miRNA with the AllPrep DNA/RNA FFPE Kit? FAQ ID -2348 View Is it good to pool multiple RNA replicates to detect expression changes that are consistently reproducible? FAQ ID -2663 View Is it necessary to clean a processor normally used for formalin-fixed tissue before using it with PAXgene Tissue fixed tissue? FAQ ID -3034 View Is it necessary to clean a processor normally used for formalin-fixed tissue before using it with PAXgene Tissue fixed tissue? FAQ ID - 3607 View Is it necessary to clean up cDNA prepared with the QuantiTect Whole Transcriptome Kit? FAQ ID -1610 View Is it necessary to include a genomic DNA removal step with the QuantiTect Whole Transcriptome Kit? FAQ ID -1614 View Is it necessary to perform calibration steps for the use of the MAX dye in duplex RT-PCR experiments on different cyclers? FAQ ID -2373 View Is it necessary to place the lids of the elution tube and column into the slots of the rotor adapter during processing on the QIAcube Connect? FAQ ID - 141496 View Is it necessary to place the lids of the elution tube and column into the slots of the rotor adapter during processing on the QIAcube? FAQ ID -1415 View Is it necessary to place the lids of the elution tube and column into the slots of the rotor adapter during processing on the QIAcube? 148736 View Is it necessary to reanalyze a plate with VPF (Volume Precision Factor) that was already processed using a QIAcuity instrument that was purchased in 2020? 3771 View Is it normal that the binding capacity of GST-resins decreases with flow rate? FAQ ID -2174 View Is it possible to adjust the Lysis Buffer volume used with the Qproteome Bacterial Protein Preparation Kit? FAQ ID -841 View Is it possible to archive PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue blocks? FAQ ID -2524 View Is it possible to archive PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue? FAQ ID -3038 View Is it possible to buy the EZ Competent Cells of the QIAGEN PCR Cloning Plus Kit separately? FAQ ID -763 View Is it possible to change voltage set-up from 110V to 230V on the QIAcuity instruments? 3761 View Is it possible to clean up a methylation reaction containing bisulfite with QIAquick Cleanup Kits? FAQ ID -519 View Is it possible to cleave the 6xHis-tag from an expressed protein? FAQ ID -140 View Is it possible to collect intact mitochondria before or after the density gradient centrifugation step of the Qproteome Mitochondria Isolation protocol? FAQ ID -1187 View Is it possible to edit the sample sheet after the run in the QIAquant Software? 153955 View Is it possible to edit the sample sheet in the Rotor-Gene Q software after a PCR run? FAQ ID -2092 View Is it possible to elute plasmid DNA from the QIAprep Spin Miniprep columns with buffer containing Potassium Phosphate? FAQ ID -854 View Is it possible to elute plasmid DNA obtained with the QuickLyse Miniprep Kit in nuclease-free water? FAQ ID -1355 View Is it possible to embed PAXgene Tissue fixed and stabilized samples in Optimal Cutting Temperature (OCT) medium for freezing? FAQ ID -2525 View Is it possible to establish an internet connection with QIAquant instrument? 153952 View Is it possible to extract RNA from frozen blood? FAQ ID -3119 View Is it possible to extract RNA from RNAprotect-stabilized-blood? FAQ ID -3120 View Is it possible to fix over a weekend? FAQ ID -3027 View Is it possible to get a Custom RT2 Profiler PCR Array, cat. # 330131, with primer assays targeting transcripts of different species, e.g. assays targeting transcripts from mouse and assays targeting transcripts from human, on one plate? FAQ ID - 3710 View Is it possible to import a standard curve from a previous PCR run on the Rotor-Gene Q? FAQ ID -2093 View Is it possible to import a standard curve from a previous run? 153947 View Is it possible to increase nucleic acid concentrations when following protocols on the BioRobot EZ1? FAQ ID -1232 View Is it possible to increase the amount of sample to obtain higher signals using the Investigator IDplex GO! Kit? FAQ ID -3090 View Is it possible to integrate the PAXgene Tissue STABILIZER into the processing? FAQ ID -3037 View Is it possible to integrate the PAXgene Tissue STABILIZER into tissue processing? FAQ ID - 3608 View Is it possible to interrupt the PAXgene Blood DNA kit protocol? FAQ ID - 3503 View Is it possible to introduce an affinity tag using the EasyXpress Linear Template Fab Kit? FAQ ID -2972 View Is it possible to isolate both RNA and recombinant 6xHis-tagged protein from the same sample? FAQ ID -532 View Is it possible to isolate DNA from bone marrow with the QIAamp DNA Blood Kits? FAQ ID -563 View Is it possible to isolate DNA from Saliva Stabilized in RNAprotect® Saliva Reagent? FAQ ID -2995 View Is it possible to isolate free genomic DNA using the QIAamp UltraSens Virus Kit? FAQ ID -358 View Is it possible to isolate genomic DNA or viral nucleic acids with PAXgene Blood miRNA protocol? FAQ ID - 3483 View Is it possible to isolate genomic DNA or viral nucleic acids with the QIAsymphony PAXgene Blood RNA protocol? FAQ ID - 3476 View Is it possible to isolate single stranded DNA (ssDNA) with the QIAEX II Kit from agarose or polyacrylamide gels? FAQ ID -576 View Is it possible to manually process PAXgene Tissue treated samples without using an automated processor? FAQ ID -3036 View Is it possible to measure the proteolytic activity of proteins on the surface of exosomes that were isolated with ExoEasy? FAQ ID - 3599 View Is it possible to microdissect PAXgene Tissue fixed, paraffin-embedded (PFPE) and PAXgene Tissue fixed, cryo-embedded (PFCE) tissues? FAQ ID -2531 View Is it possible to modify QIAsymphony protocols? FAQ ID -1903 View Is it possible to modify the transduction protocol for Cignal Lenti Reporter Assays to save some pipetting steps? FAQ ID - 3709 View Is it possible to normalize to more or less than 500 ng with the SeqTarget System if needed? FAQ ID -2235 View Is it possible to partially use RNeasy 96 plates and use the plate multiple times? FAQ ID -3124 View Is it possible to perform a UNG treatment when using QuantiTect kits? FAQ ID -564 View Is it possible to perform immunoprecipitation (IP) directly on protein fractions from the Qproteome Cell Compartment Kit? FAQ ID -855 View Is it possible to perform laser microdissection with tissues stabilized with RNAprotect Tissue reagent? FAQ ID -608 View Is it possible to place the PAXgene Saliva Collector tube directly on the QIAsymphony SP instrument sample rack? 3825 View Is it possible to process formalin-fixed and PAXgene Tissue fixed samples together in one run? FAQ ID -3033 View Is it possible to process formalin-fixed and PAXgene Tissue fixed samples together in one run? FAQ ID - 3606 View Is it possible to process volumes of saliva from the PAXgene Saliva Collector other than the 400 or 1000 µl from the standard protocols for the QIAsymphony DNA Midi Kit using the QIAsymphony SP instrument? 3824 View Is it possible to purchase Buffer VXL which is part of the QIAamp cador Pathogen Mini Kit, (Cat No.: 54104, 54106) separately? FAQ ID - 3712 View Is it possible to purify a GST-tagged protein under denaturing conditions using Glutathione Superflow? FAQ ID -2172 View Is it possible to purify and normalize long range PCRs produced with PCR kits from other vendors for the SeqTarget System? FAQ ID -2243 View Is it possible to purify DNA from the whole sample of a PAXgene Saliva Collector in one preparation? 3826 View Is it possible to purify viral RNA using FastLane Cell RT-PCR Kits? FAQ ID -1484 View Is it possible to quantify protein content in RLT lysates? FAQ ID -3155 View Is it possible to run therascreen mutation or therascreen Pyro kits on PyroMark Q24 Advanced system? FAQ ID -3161 View Is it possible to scale up QuantiTect Reverse Transcription reactions to allow use of larger amounts of RNA? FAQ ID -1063 View Is it possible to scale up sample volume and reaction volume? FAQ ID - 3884 View Is it possible to simultaneously isolate DNA and RNA from Saliva Stabilized in RNAprotect® Saliva Reagent? FAQ ID -2996 View Is it possible to stop the DNeasy tissue protocol and store the tissue lysates after digesting in buffer ATL and Proteinase K? FAQ ID - 3362 View Is it possible to store nucleic acids extracted using the SpeedXtract Virus Kit? FAQ ID - 3680 View Is it possible to store TAGzyme enzymes at°C? -80 View Is it possible to store the lysates? How can the lysates be stored? FAQ ID - 3973 View Is it possible to synthesize membrane proteins using the EasyXpress Protein Synthesis Kit? FAQ ID -602 View Is it possible to test the efficiency of FastSelect rRNA removal by using a Bioanalyzer, etc.? FAQ ID -147916 View Is it possible to transfect DNA into insect cell lines such as Drosophila melanogaster S2? FAQ ID -397 View Is it possible to upgrade existing PyroMark Q24 instruments to PyroMark Q24 Advanced or do I have to purchase a new instrument? FAQ ID -3162 View Is it possible to use a QIAzol lysate for the isolation of RNA from tissue on the BioRobot EZ1? FAQ ID -573 View Is it possible to use a standard processor — the kind used routinely for formalin-fixed samples — for dehydration and paraffin infiltration of PAXgene Tissue treated samples? FAQ ID -3032 View Is it possible to use ABI instruments without ROX as a passive reference? FAQ ID - 3889 View Is it possible to use different sample types in one run with the MagAttract 96 cador Pathogen Kit? FAQ ID -2072 View Is it possible to use formalin-fixed, paraffin-embedded (FFPE) kits and protocols for extraction of biomolecules from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue? FAQ ID -3043 View Is it possible to use formalin-fixed, paraffin-embedded (FFPE) kits and protocols to isolate biomolecules from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue? FAQ ID - 3610 View Is it possible to use frozen cell pellets with the FastLane Cell cDNA Kit? FAQ ID -835 View Is it possible to use less than 1 mg of protein with the PhosphoProtein Purification Kit? FAQ ID -599 View Is it possible to use LiquiChip beads for FACS applications? FAQ ID -733 View Is it possible to use lower siRNA concentrations with FlexiTube siRNA Premix? FAQ ID -2264 View Is it possible to use Lyse&Spin baskets? FAQ ID - 143762 View Is it possible to use MaXtract for an organic extraction with a mixture of phenol and BCP (1 bromine chlorpropane)? -3 View Show more results Back to top Contact QIAGEN Global contacts Technical Service Customer Care

Do you have a protocol for evaluating temperature accuracy of the BioRobot EZ1?

FAQ ID -1168

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Do you have a protocol for GMO testing of food samples?

FAQ ID -921

View

Do you have a protocol for high-throughput purification of plasmid DNA using the BioRobot Universal System?

FAQ ID -1191

View

Do you have a protocol for isolating cytoplasmic RNA from animal cells using the RNeasy Mini Kit?

FAQ ID -1207

View

Do you have a protocol for isolating RNA from yeast using RNeasy?

FAQ ID -535

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Do you have a protocol for isolation of bacterial DNA from soil?

FAQ ID -923

View

Do you have a protocol for isolation of genomic and plasmid DNA from cell cultures using QIAGEN Genomic-tips?

FAQ ID -902

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Do you have a protocol for isolation of genomic and viral DNA from lymphocytes using QIAGEN Genomic-tips?

FAQ ID -901

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Do you have a protocol for isolation of genomic DNA from dried blood spots using the QIAamp 96 DNA Blood Kit?

FAQ ID -919

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Do you have a protocol for isolation of genomic DNA from flies using QIAGEN Genomic-tips?

FAQ ID -903

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Do you have a protocol for isolation of genomic DNA from insects?

FAQ ID -904

View

Do you have a protocol for isolation of genomic DNA from plants and filamentous fungi using QIAGEN Genomic-tips?

FAQ ID -905

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Do you have a protocol for isolation of genomic DNA from saliva and mouthwash?

FAQ ID -917

View

Do you have a protocol for isolation of genomic DNA from saliva using the DNeasy Blood & Tissue Kit?

FAQ ID -931

View

Do you have a protocol for isolation of plasmid DNA using the Sigma Centrifuge 4?

-15

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Do you have a protocol for long-term, siRNA-mediated gene silencing using HiPerFect Transfection Reagent?

FAQ ID -972

View

Do you have a protocol for manual purification of 6xHis-tagged proteins expressed in E. coli using Ni-NTA Superflow?

FAQ ID -967

View

Do you have a protocol for obtaining > 20 µg plasmid DNA using the QIAprep Spin Miniprep Kit?

FAQ ID -3102

View

Do you have a protocol for performing quantitative multiplex PCR on the LightCycler 2.0 system?

FAQ ID -966

View

Do you have a protocol for polyacrylamide gel analysis of oligonucleotides?

FAQ ID -961

View

Do you have a protocol for purification of cytoplasmic RNA from animal cells?

FAQ ID -1257

View

Do you have a protocol for purification of DNA fragments from Cy3/Cy5 dye-label reactions?

FAQ ID -947

View

Do you have a protocol for purification of DNA from clotted blood using the FlexiGene DNA Kit?

FAQ ID -973

View

Do you have a protocol for purification of DNA from cultured animal cells using the DNeasy 96 Blood & Tissue Kit?

FAQ ID -934

View

Do you have a protocol for purification of DNA from stool samples for pathogen detection using BioSprint Instruments?

FAQ ID -1165

View

Do you have a protocol for purification of DNA from stool samples using BioSprint Instruments?

FAQ ID -1166

View

Do you have a protocol for purification of genomic DNA from cultured cells using the QIAamp DNA Micro Kit?

FAQ ID -1547

View

Do you have a protocol for purification of miRNA from animal cells using the RNeasy Plus Mini Kit and RNeasy MinElute Cleanup Kit?

FAQ ID -1258

View

Do you have a protocol for purification of total DNA from crude lysates?

FAQ ID -1255

View

Do you have a protocol for purification of total DNA from insects?

FAQ ID -1254

View

Do you have a protocol for purification of total DNA from yeast?

FAQ ID -1253

View

Do you have a protocol for purification of total RNA from fatty tissues using QIAzol Lysis Reagent and MaXtract High Density?

FAQ ID -1550

View

Do you have a protocol for purification of total RNA from plant latex?

FAQ ID -1694

View

Do you have a protocol for purification of total RNA from plant tissue with the RNeasy Lipid Tissue Mini Kit?

FAQ ID -1693

View

Do you have a protocol for purifying 2x96 PCR samples simultaneously with the QIAquick 96 PCR Purification Kit?

FAQ ID -935

View

Do you have a protocol for radioactive labelling of cDNA?

FAQ ID -960

View

Do you have a protocol for rapid purification of archive-quality DNA from up to 9 x 10e6 cells using Gentra Puregene Kits?

FAQ ID -1969

View

Do you have a protocol for reverse transfection of adherent cells with siRNA in 384-well plates?

FAQ ID -971

View

Do you have a protocol for Rotor-Gene software setup for the Rotor-Gene SYBR Green PCR and RT-PCR Kits?

FAQ ID -2104

View

Do you have a protocol for Streptokinase treatment of clotted blood?

FAQ ID -912

View

Do you have a protocol for the AllPrep DNA/RNA/Protein Mini Kit on the QIAcube Connect?

FAQ ID - 141506

View

Do you have a protocol for the detection of Bordetella pertussis DNA by PCR?

FAQ ID -914

View

Do you have a protocol for the fixation of cells transfected with fluorescently labeled siRNA?

FAQ ID -793

View

Do you have a protocol for the isolation of BAC DNA using the QIAGEN Plasmid Midi Kit?

FAQ ID -881

View

Do you have a protocol for the isolation of bacteriophage P1 derived constructs with any of your plasmid kits?

FAQ ID -883

View

Do you have a protocol for the isolation of DNA from buccal cells using the BioRobot EZ1?

FAQ ID -1260

View

Do you have a protocol for the isolation of DNA from buffy coat using the BioRobot EZ1?

FAQ ID -985

View

Do you have a protocol for the isolation of DNA from cultured cells using the BioRobot EZ1?

FAQ ID -990

View

Do you have a protocol for the isolation of DNA from epithelial cells mixed with sperm cells?

FAQ ID -926

View

Do you have a protocol for the isolation of DNA from forensic samples using the BioRobot M48?

FAQ ID -1017

View

Do you have a protocol for the isolation of DNA from formalin-preserved stool samples?

FAQ ID -922

View

Do you have a protocol for the isolation of DNA from fungi using the BioRobot EZ1?

FAQ ID -992

View

Do you have a protocol for the isolation of DNA from mouse tails using MagAttract technology on BioRobot M96?

FAQ ID -1007

View

Do you have a protocol for the isolation of DNA from soft tissues using the TissueLyser?

FAQ ID -924

View

Do you have a protocol for the isolation of DNA from tofu?

FAQ ID -932

View

Do you have a protocol for the isolation of genomic DNA and/or proteins from fatty tissue treated with QIAzol?

FAQ ID -955

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Do you have a protocol for the isolation of genomic DNA from bone?

FAQ ID -908

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Do you have a protocol for the isolation of genomic DNA from frozen clotted blood?

FAQ ID -900. Test

View

Do you have a protocol for the isolation of genomic DNA from fungi?

FAQ ID -911

View

Do you have a protocol for the isolation of genomic DNA from paraffin-embedded samples on the BioRobot M48?

FAQ ID -1019

View

Do you have a protocol for the isolation of genomic DNA from sperm?

FAQ ID -909

View

Do you have a protocol for the isolation of genomic DNA from whole blood for use in MRC-Holland MLPA® assays?

FAQ ID -1169

View

Do you have a protocol for the isolation of genomic DNA from yeast/fungi directly from Blood?

FAQ ID -3150

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Do you have a protocol for the isolation of plasmid DNA from Agrobacterium?

FAQ ID -898

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Do you have a protocol for the isolation of plasmid DNA from Bacillus subtilis?

FAQ ID -896

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Do you have a protocol for the isolation of plasmid DNA from Borrelia spp.?

FAQ ID -884

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Do you have a protocol for the isolation of plasmid DNA from Citrobacter freundii?

FAQ ID -885

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Do you have a protocol for the isolation of plasmid DNA from Corynebacterium glutamicum?

FAQ ID -886

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Do you have a protocol for the isolation of plasmid DNA from Lactobacillus spp.?

FAQ ID -887

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Do you have a protocol for the isolation of plasmid DNA from Oligotropha carboxidovorans?

FAQ ID -888

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Do you have a protocol for the isolation of plasmid DNA from Proteus spp.?

FAQ ID -889

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Do you have a protocol for the isolation of plasmid DNA from Staphylococcus spp.?

FAQ ID -890

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Do you have a protocol for the isolation of plasmid DNA from yeast?

FAQ ID -853

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Do you have a protocol for the isolation of RNA from bacterial cultures using RNAprotect Bacteria Reagent and QIAzol Lysis Reagent?

FAQ ID -1020

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Do you have a protocol for the isolation of RNA from leukocytes in milk?

FAQ ID -952

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Do you have a protocol for the isolation of single-stranded DNA from M13 phage using QIAGEN Plasmid Kits?

FAQ ID -894

View

Do you have a protocol for the isolation of total nucleic acids from animal and human cells on the BioRobot EZ1?

FAQ ID -974

View

Do you have a protocol for the isolation of total nucleic acids from animal and human tissues on the BioRobot EZ1?

FAQ ID -975

View

Do you have a protocol for the isolation of total nucleic acids from cells on the M48 BioRobot?

FAQ ID -1009

View

Do you have a protocol for the isolation of total nucleic acids from tissue on the M48 BioRobot?

FAQ ID -1008

View

Do you have a protocol for the isolation of total RNA from buccal swabs?

FAQ ID -956

View

Do you have a protocol for the isolation of total RNA from paraffin-embedded sections on the BioRobot EZ1?

FAQ ID -976

View

Do you have a protocol for the isolation of total RNA from paraffin-embedded sections on the Biorobot M48?

FAQ ID -1733

View

Do you have a protocol for the isolation of total RNA from plant tissue using QIAGEN-tips?

FAQ ID -949

View

Do you have a protocol for the isolation of total RNA from plants using the RNeasy 96 Kit?

FAQ ID -951

View

Do you have a protocol for the isolation of very low-copy plasmids from Streptomyces spp.?

FAQ ID -893

View

Do you have a protocol for the isolation of viral DNA from stool?

FAQ ID -929

View

Do you have a protocol for the isolation of viral RNA from stool?

FAQ ID -918

View

Do you have a protocol for the preparation of tissues preserved in RNAprotect Tissue Reagent for pathological studies?

FAQ ID -950

View

Do you have a protocol for the purification of 6xHis-tagged proteins using BioSprint?

FAQ ID -1167

View

Do you have a protocol for the purification of archive-quality DNA from 0.5–20 mg paraffin-embedded tissue using Gentra Puregene Kits?

FAQ ID -1964

View

Do you have a protocol for the purification of archive-quality DNA from 1 ml of whole blood with the Gentra Puregene Tissue Kit?

FAQ ID -1953

View

Do you have a protocol for the purification of archive-quality DNA from 100 to 5 x 10e8 cultured cells using Gentra Puregene Kits?

FAQ ID -1960

View

Do you have a protocol for the purification of archive-quality DNA from blood smears using Gentra Puregene Kits?

FAQ ID -1948

View

Do you have a protocol for the purification of archive-quality DNA from buccal brushes using Gentra Puregene Kits?

FAQ ID -1965

View

Do you have a protocol for the purification of archive-quality DNA from buffy coat using Gentra Puregene Kits?

FAQ ID -1955

View

Do you have a protocol for the purification of archive-quality DNA from cerebrospinal fluid (CSF) using Gentra Puregene Kits?

FAQ ID -1967

View

Do you have a protocol for the purification of archive-quality DNA from Chlamydomonas using Gentra Puregene Kits?

FAQ ID -1968

View

Do you have a protocol for the purification of archive-quality DNA from Eimeria oocysts using Gentra Puregene Kits?

FAQ ID -1952

View

Do you have a protocol for the purification of archive-quality DNA from fecal cells using Gentra Puregene Kits?

FAQ ID -1972

View

Do you have a protocol for the purification of archive-quality DNA from fish using Gentra Puregene Kits?

FAQ ID -1973

View

Do you have a protocol for the purification of archive-quality DNA from fungus using Gentra Puregene Kits?

FAQ ID -1971

View

Do you have a protocol for the purification of archive-quality DNA from Gram-negative bacteria using Gentra Puregene Kits?

FAQ ID -1975

View

Do you have a protocol for the purification of archive-quality DNA from Gram-positive bacteria using Gentra Puregene Kits?

FAQ ID -1946

View

Do you have a protocol for the purification of archive-quality DNA from hair roots using Gentra Puregene Kits?

FAQ ID -1947

View

Do you have a protocol for the purification of archive-quality DNA from human dried blood using Gentra Puregene Kits?

FAQ ID -1959

View

Do you have a protocol for the purification of archive-quality DNA from marine invertebrate tissue using Gentra Puregene Kits?

FAQ ID -1950

View

Do you have a protocol for the purification of archive-quality DNA from nematodes using Gentra Puregene Kits?

FAQ ID -1951

View

Do you have a protocol for the purification of archive-quality DNA from tissue fixed in ethanol or formalin using Gentra Puregene Kits?

FAQ ID -1974

View

Do you have a protocol for the purification of archive-quality DNA from umbilical cord blood using Gentra Puregene Kits?

FAQ ID -1958

View

Do you have a protocol for the purification of archive-quality DNA from whole blood using Gentra Puregene Kits?

FAQ ID -1954

View

Do you have a protocol for the purification of DNA from buccal swabs using the BioSprint System?

FAQ ID -983

View

Do you have a protocol for the purification of DNA from cells using the BioSprint System?

FAQ ID -977

View

Do you have a protocol for the purification of DNA from dried blood spots using the BioSprint System?

FAQ ID -982

View

Do you have a protocol for the purification of DNA from fruit flies?

FAQ ID -1970

View

Do you have a protocol for the purification of DNA from rodent tail using the BioSprint System?

FAQ ID -979

View

Do you have a protocol for the purification of PCR products using the BioSprint System?

FAQ ID -984

View

Do you have a protocol for the QIAprep Spin M13 Kit on the QIAcube Connect?

FAQ ID - 1414507

View

Do you have a protocol for the removal of endotoxins from already purified plasmid DNA?

FAQ ID -500

View

Do you have a protocol for transfection of adherent cells with low concentrations of siRNA using RNAiFect Transfection Reagent?

FAQ ID -970

View

Do you have a protocol for transfection of differentiated macrophage cell lines (THP) with siRNA?

-1

View

Do you have a protocol for transfection of macrophage cell lines (J774.A1 and RAW 264.7) with siRNA?

FAQ ID -1251

View

Do you have a protocol for transfection of suspension cell lines (Jurkat and K562) with siRNA?

FAQ ID -1250

View

Do you have a protocol for transient transfection of cells in 96-well plates using PolyFect Transfection Reagent?

FAQ ID -1013

View

Do you have a protocol for transient transfection of HEK 293 cells in 75 cm3 flasks?

FAQ ID -1014

View

Do you have a protocol for transient transfection of HeLa-S3 cells in 60 mm dishes using PolyFect?

FAQ ID -1012

View

Do you have a protocol for transient transfection of Hela-S3 cells using PolyFect Transfection Reagent?

FAQ ID -997

View

Do you have a protocol for vacuum processing of blood samples with the QIAamp DNA Blood Midi or Maxi Kits?

FAQ ID -1011

View

Do you have a protocol to isolate RNA from blood and bone marrow using the BioRobot EZ1?

FAQ ID -567

View

Do you have an antibody for Western Blot Analysis of MAPK1 gene knockdown using your MAPK1 control siRNA?

FAQ ID -746

View

Do you have an in vitro translation kit allowing the incorporation of seleno-methionine for X-Ray crystallography based protein analysis?

FAQ ID -879

View

Do you have an off-the shelf diagnostics kit for WGA?

FAQ ID -678

View

Do you have any information or guidelines regarding the choice of reference genes for real-time PCR?

FAQ ID -2371

View

Do you have any recommendations for using the PosphoProtein Kit with starting material other than cells?

FAQ ID -597

View

Do you have any recommendations on how to process my AAV samples?

FAQ ID - 3860

View

Do you have data on the DNA yield at different incubation time for the Repli-G Kits?

FAQ ID -3113

View

Do you have data showing effects of sample size on DNA yield and purity using the DNeasy 96 Blood & Tissue kit?

FAQ ID -129

View

Do you have data to show lowest dropout rate with Repli-G WTA Single Cells kit?

FAQ ID - 3392

View

Do you have FACS or Fluoro-Microscopy data using HiPerFect HTS Reagent for delivery of siRNA?

FAQ ID -2557

View

Do you have information about the cleanup of single-stranded DNA (ssDNA) with QIAquick columns?

FAQ ID -759

View

Do you have information on DNA yields from blood with variable numbers of white blood cells using the M48 BioRobot?

FAQ ID -1006

View

Do you have information on in vivo RNA interference experiments?

FAQ ID -817

View

Do you have information on PCR template preparation from preserved blood samples on the M48 BioRobot?

FAQ ID - 1003

View

Do you have information on realtime RT-PCR performance of RNA isolated from monocytes on the M48 BioRobot?

FAQ ID -1010

View

Do you have information on the reproducibility of DNA yields from blood using the M48 BioRobot?

FAQ ID - 1004

View

Do you have information on the use of recombinant DNA and RNA as absolute standards for realtime RT-PCR?

FAQ ID -729

View

Do you have protocols for multiple extractions of DNA fragments from agarose gels?

FAQ ID -944

View

Do you have protocols for sample processing on the Autopure LS instrument?

FAQ ID -1759

View

Do you have protocols for software setup on various PCR platforms using QuantiFast Probe RT-PCR Kits?

FAQ ID -2105

View

Do you have protocols for the isolation of genomic DNA from tissue using QIAGEN-tips 2500 (Mega) and 10000 (Giga)?

FAQ ID -907

View

Do you have stability data for genomic DNA isolated with the BioSprint Kits?

FAQ ID -1318

View

Do you have stability data for genomic DNA isolated with the Flexigene DNA Kit?

FAQ ID -1317

View

Do you have stability data for genomic DNA isolated with the QIAamp DNA Blood Mini Kit?

FAQ ID -518

View

Do you have stability data for RNA isolated with PAXgene miRNA Kit?

FAQ ID - 3480

View

Do you have stability data for RNA isolated with the QIAsymphony PAXgene Blood RNA Kit?

FAQ ID -2983

View

Do you have to use a restriction enzyme to relieve ITR secondary structures and increase target accessibility?

FAQ ID - 3974

View

Do you have transfection data for QIAGEN Transfection Reagents?

FAQ ID -158

View

Do you have WGA protocols for starting materials not mentioned in the REPLI-g Handbooks?

FAQ ID -1160

View

Do you know of anything in the buffers/DNA sample that can cause bias in REPLI-g WGA?

FAQ ID -684

View

Do you need to dilute the lysates before setting up the PCR mix?

FAQ ID - 3978

View

Do you need to pre-treat food samples before DNA isolation using the QIAsymphony mericon Bacteria Kit on the QIAsymphony SP?

FAQ ID -2957

View

Do you offer a QuantiFast Kit for one-step RT-PCR?

FAQ ID -1695

View

Do you offer FlexiTube or FlexiPlate siRNAs for rat?

FAQ ID -3114

View

Do you offer QIAshredder spin columns for the RNeasy Midi/Maxi Kit format? If not, what is the alternative?

FAQ ID -560

View

Do you offer trial-kit sizes for the new QuantiFast Kits?

FAQ ID -1429

View

Do you provide control proteins for use with the PhosphoProtein Purification Kit?

FAQ ID -596

View

Do you recommend 1-step or 2-step real-time RT-PCR for gene expression analysis?

FAQ ID -1056

View

Do you recommend normalizing single cell miRNA expression data to a “housekeeping” gene or miRNA?

FAQ ID - 3580

View

Do you recommend storage of highly diluted AAV samples?

FAQ ID - 3972

View

Do you recommend storage of highly diluted mycoplasma samples?

FAQ ID - 3998

View

Do you recommend using indicator cards for buccal cells on FTA with Investigator IDplex GO! Kit?

FAQ ID -3095

View

Do you sell CoralLoad dye separately?

FAQ ID - 3368

View

Do you sell the ATP-Dependent Exonuclease of the QIAGEN Large-Construct Kit separately?

FAQ ID -825

View

Do you synthesize thiophosphate modified random hexamers?

FAQ ID -711

View

Does a QIAseq miRNA library include hY4 Y RNA?

FAQ ID - 3670

View

Does an activation time of 15 minutes influence the performance of the HotStarTaq Plus DNA Polymerase?

FAQ ID -1050

View

Does an internal control have to be included for each QIAsymphony Virus/Bacteria protocol run?

FAQ ID -2040

View

Does an internal control have to be used with the QIAsymphony Virus Blood 200 Protocol?

FAQ ID -2162

View

Does anything have to be added to express post-translational modifications (PTMs) with the EasyXpress Insect Kit II?

FAQ ID -1226

View

Does Buffer APP in the AllPrep DNA/RNA/Protein Mini Kit contain acetone?

FAQ ID -1576

View

Does carrier RNA have to be added using the QIAsymphony Virus Blood 200 Protocol?

FAQ ID -2163

View

Does carrier RNA interfere with the QuantiTect Whole Transcriptome amplification?

FAQ ID -3080

View

Does cDNA amplified with the QuantiTect Whole Transcriptome Kit correspond to full-length RNA transcripts?

FAQ ID -1585

View

Does DEPC harm RNA?

FAQ ID -529

View

Does endogenously expressed DAPase in human cells degrade proteins that are expressed in cell culture?

FAQ ID -527

View

Does ethanol from the PCR product purification influence the normalization procedure for the SeqTarget System?

FAQ ID -2244

View

Does GelPilot DNA Loading Dye contain ethidium bromide?

FAQ ID -1286

View

Does HiPerFect work for the transfection of miScript miRNA mimics and inhibitors?

FAQ ID -1984

View

Does QIAGEN guarantee the performance of the SureSilencing shRNA Plasmids?

FAQ ID -2787

View

Does QIAGEN offer a design of Custom PyroMark CpG Assays?

FAQ ID -2818

View

Does QIAGEN offer an anti-GFP gapmer control?

FAQ ID - 3739

View

Does QIAGEN offer vectors for direct cloning of PCR products?

FAQ ID -146

View

Does QIAGEN offers arrays and assays configured specifically for QIAquant instrument?

153929

View

Does QIAGEN provide template files for GeneMapper® 5 software?

FAQ ID -3177

View

Does QIAGEN sell 6xHis-tagged proteins besides the 6xHis Protein Ladder?

FAQ ID -594

View

Does QIAGEN sell a vector with a T7 promotor for use with the EasyXpress Protein Synthesis Insect Kit?

FAQ ID -937

View

Does QIAGEN sell Q-Solution separately?

FAQ ID -204

View

Does REPLI-g technology for whole genome amplification work with paraffin embedded samples?

FAQ ID -673

View

Does residual Allprotect Tissue Reagent left behind on tissue samples interfere with DNA, RNA, and protein purification?

FAQ ID -1573

View

Does RNAprotect affect morphology of tissues?

FAQ ID -3142

View

Does RNase-free DNase I affect the isolation of dsRNA?

FAQ ID -3153

View

Does salt concentration in the food matter when using the DNeasy mericon Food Kit?

FAQ ID - 3346

View

Does SYBR Green dye present in QuantiTect kits interfere with ethidium bromide staining?

FAQ ID -312

View

Does the 5x OneStep RT-PCR Buffer contain BSA?

FAQ ID -326

View

Does the addition of sample and organic solution to the MaXtract Tubes require a specific pipetting sequence?

FAQ ID -1307

View

Does the amount of Internal Control to be spiked into the lysis buffer or lysate depend on the elution volume only?

FAQ ID -2604

View

Does the atypical look of the lyophilized FlexiTube siRNA Premix have a negative impact on performance?

FAQ ID -2267

View

Does the centrifugal force have any effect on the kinetics of a PCR reaction on the Rotor-Gene Q?

FAQ ID -2081

View

Does the DNA extracted using the PAXgene Blood DNA System contain RNA?

FAQ ID - 3499

View

Does the EasyXtal Tool conform to an SBS format?

FAQ ID -1115

View

Does the Epitect Bisulfite Kit also work on DNA extracted from plants?

FAQ ID -1209

View

Does the high primer concentration required by QuantiFast SYBR Green PCR Kits impair annealing specificity?

FAQ ID -1444

View

Does the His-tag interfere with the purification procedure of the QIAGEN PhosphoProtein Purification Kit?

FAQ ID -598

View

Does the insert sequence provided in the specification sheet correspond to the target sequence or to anti-sense sequence?

FAQ ID - 3627

View

Does the KRAS PCR kit cover the codon 61 mutation?

FAQ ID -2308

View

Does the master mix in the QuantiFast Kits contain dUTP to allow UNG treatments?

FAQ ID -1431

View

Does the miScript Plant RT Kit contain controls?

FAQ ID - 3435

View

Does the miScript Single Cell qPCR Kit contain controls?

FAQ ID - 3574

View

Does the miScript Target Protector match the target sequence?

FAQ ID -2250

View

Does the PAXgene Tissue RNA Kit co-purify small RNAs?

FAQ ID -3048

View

Does the PAXgene Tissue RNA Kit co-purify small RNAs?

FAQ ID - 3615

View

Does the PyroMark Assay Design or application software give any cycling conditions for individual assay primers or a PCR setup pipetting scheme?

FAQ ID -2862

View

Does the PyroMark CpG LINE assay target mouse transposons as well?

-1

View

Does the PyroMark LINE assay target all LINE sequences?

2860

View

Does the QIAquant Software have the capacity to export data? Which data formats can be used for the export?

153946

View

Does the QIAsymphony mericon Bacteria Kit contain an extraction control?

FAQ ID -2961

View

Does the QIAsymphony PAXgene Blood ccfDNA Kit also allow eluting ccfDNA in less than 60 µl?

FAQ ID - 3656

View

Does the QIAsymphony SP system generate run reports and/or log files?

FAQ ID -1911

View

Does the QuantiTect Whole Transcriptome Kit work with RNA purified from bacteria, yeast, or plants?

FAQ ID -1615

View

Does the REPLI-g Single Cell DNA Library Kit include a size selection step?

FAQ ID - 3539

View

Does the REPLI-g Single Cell DNA Library Kit include adapters?

FAQ ID - 3538

View

Does the REPLI-g Single Cell RNA Library Kit include a size selection step?

FAQ ID - 3551

View

Does the REPLI-g Single Cell RNA Library Kit include adapters?

FAQ ID - 3550

View

Does the reuse of the Waste Tubes increase the risk of cross-contamination?

FAQ ID - 3988

View

Does the Rotor-Gene Q software have the capacity to export data?

FAQ ID -2089

View

Does the same U1 promoter in the SureSilencing shRNA Plasmids express shRNA in human, mouse, and rat cell lines?

FAQ ID -2887

View

Does the Type-it Microsatellite PCR Kit always require an annealing temperature of 60°C, or can pre-optimized annealing temperatures for target-primer systems be used?

FAQ ID -2061

View

Does the use of MAPK1 as a control in siRNA transfection experiments have secondary effects on the cell culture?

FAQ ID -548

View

Does this method with the EpiTect Methyl qPCR Array detect methylation at specific CpG dinucleotides?

FAQ ID -2737

View

Does TopTaq Stabilizer of the TopTaq DNA Polymerase and Master Mix Kits interfere with downstream applications?

FAQ ID -1744

View

dPCR results are in copies per microliter. How do I calculate host cell DNA contamination in femtogram per microliter?

FAQ ID - 3876

View

During data analysis, how should the threshold be set in the Yellow channel on the Rotor-Gene Q cycler to analyze the Internal Control from the QuantiFast Pathogen PCR +IC Kit or the QuantiFast Pathogen RT-PCR +IC Kit

FAQ ID -2607

View

Following thermal cycling, reaction volume has evaporated from some of the wells of my qPCR assay plate. What should I do?

FAQ ID -2683

View

For how long are BioMag Particles stable?

FAQ ID -262

View

For sequencing application, does the lysate require purification?

3792

View

For the QuantiFast Pathogen RT-PCR +IC kit, does the Internal Control also control for the reverse transcription reaction?

FAQ ID -2601

View

For the Sanger analysis tool, the customer needs to upload two .abi1 files of forward and reverse sequences? Is there any additional info that needs to be provided?

3796

View

For the the QIAprep 96 Plus Miniprep Kit or the QIAGEN Plasmid Plus 96 Miniprep Kit, can plates be partially used?

FAQ ID -2319

View

For what type of sample material is the QIAsymphony PAXgene Blood ccfDNA Kit intended?

FAQ ID - 3652

View

For which applications can the RNA isolated with the RNeasy Plus Universal Kit also be used?

FAQ ID -2339

View

Has anyone verified whole genome amplification accuracy with Sequencing?

FAQ ID -701

View

Has QIAGEN ever tested Proteinase K treatment for a time period longer than overnight?

View

Has RNAi been successful using siRNA in Zebrafish and Xenopus?

FAQ ID -400

View

Have primer sequences in the Investigator IDplex GO! Kit been changed compared to the Investigator IDplex Plus assay?

FAQ ID -3092

View

Have QuantiTect Primer Assays been tested with QuantiFast SYBR Green PCR Kits on the Mastercycler ep realplex?

FAQ ID -1714

View

Have you observed co-amplification of genomic DNA from RNA templates used in the QuantiTect Whole Transcriptome Procedure?

FAQ ID -1619

View

Have you tested the effect of inhibitors on PCR performance?

FAQ ID -818

View

How accurate and reliable is PyroMark Q24 in mutation analysis?

FAQ ID -2094

View

How are NeXtal deep-well blocks sealed? Can they be resealed?

FAQ ID -1128

View

How are plasma membrane containing vesicles eluted off the ligand in the Qproteome Plasma Membrane Protein procedure?

FAQ ID -1203

View

How are siRNAs introduced into C. elegans?

FAQ ID -396

View

How are the different mutations for a single gene normalized on the qBiomarker Somatic Mutation PCR array?

FAQ ID -2428

View

How are the genes in each iPSC qBiomarker PCR Array selected?

FAQ ID -2439

View

How are the primers in the EpiTect Methyl qPCR Array designed?

FAQ ID -2735

View

How are the PyroMark CpG Assays reconstituted?

FAQ ID -2817

View

How are the PyroMark CpG Assays shipped and stored?

FAQ ID -2816

View

How are the supernatants resulting from the first two spin steps of the PAXgene Bone Marrow RNA kit purification procedure disposed?

FAQ ID -2516

View

How are the SureSilencing shRNA Plasmids shipped?

FAQ ID -2884

View

How best to handle the light sensitive LiquiChip reagents?

FAQ ID -735

View

How can a C-terminal 6xHis-tagged QIAgene be expressed?

FAQ ID -2041

View

How can a clogged capillary channel be identified when running samples on the QIAxcel System or QIAxcel Advanced?

FAQ ID -1836

View

How can a positive threshold be set when running the QIAxcel System?

FAQ ID -1843

View

How can air bubbles in PhosphoProtein Purification Columns be avoided?

FAQ ID -1070

View

How can foaming of Buffer RLT Plus lysates be avoided when using RNeasy Plus Micro and Mini Kits, or the Allprep DNA/RNA Mini Kit?

FAQ ID -1734

View

How can full-length genes or individual domains of the QIAgenes Insect/Mammalia constructs be subcloned?

FAQ ID -2051

View

How can I adjust the ROX concentration in the QuantiFast Probe RT-PCR Plus master mix?

FAQ ID -2356

View

How can I analyze proteins from exosomes and other extracellular vesicles (EVs) obtained with the exoEasy Maxi Kit by SDS-PAGE?

FAQ ID - 3707

View

How can I avoid little or no RNA yields when using an RNeasy Kit?

FAQ ID -28

View

How can I avoid or remove genomic DNA contamination from the total RNA preparation?

FAQ ID -2662

View

How can I avoid poor immunolocalization morphology with Anti-His Antibodies?

FAQ ID -200

View

How can I avoid primer-dimer formation during PCR amplification?

FAQ ID -544

View

How can I avoid variable transfection efficiencies with SuperFect Reagent?

FAQ ID -179

View

How can I be sure that I am harvesting my induced bacterial culture at the best time point for protein expression?

FAQ ID -788

View

How can I be sure that the restriction enzyme digestion is complete in the EpiTect Methyl qPCR system?

FAQ ID -2731

View

How can I calculate the number of bacterial cells that are present in a sample using the Microbial DNA qPCR Assays?

FAQ ID — 3411

View

How can I check DNA sample quality before using the Somatic Mutation PCR Arrays & Assays?

FAQ ID -2430

View

How can I check for purity of RNA isolated using RNeasy Kits?

FAQ ID -1023

View

How can I check if any residual proteins remain on the Ni-NTA Agarose matrix after elution?

FAQ ID -324

View

How can I check the integrity of RNA purified using RNeasy Kits?

FAQ ID -1024

View

How can I check the quality or size of my extracted circulating cell-free DNA?

FAQ ID - 3705

View

How can I check the success of the normalization procedure for the SeqTarget System?

FAQ ID -2245

View

How can I create a report/logfile on my QIAsymphony SP system?

FAQ ID - 3594

View

How can I decontaminate my QIAsymphony SP / AS system?

FAQ ID - 3714

View

How can I decontaminate the BioRobot EZ1?

FAQ ID -1231

View

How can I decontaminate the QIAcube Connect MDx system?

148739

View

How can I decontaminate the QIAcube Connect system?

FAQ ID - 141500

View

How can I decontaminate the QIAcube system?

FAQ ID -1419

View

How can I decrease ribosomal RNA (rRNA) contamination using Oligotex?

FAQ ID -642

View

How can I desalt and concentrate a protein fraction by trichloroacetic acid (TCA) precipitation?

FAQ ID -2954

View

How can I determine if a tube is suitable for clot detection on QIAsymphony?

FAQ ID - 3590

View

How can I determine the quality of my REPLI-g amplified products?

FAQ ID -695

View

How can I determine whether amplification occurs from mRNA-derived cDNA or from genomic DNA contamination?

FAQ ID -2687

View

How can I eliminate contaminating protein in my Ni-NTA 6xHis-tag protein purification?

FAQ ID -102

View

How can I ensure complete genomic DNA removal when using the RNase-Free DNase Set?

FAQ ID -1087

View

How can I ensure optimal performance of my QIAGEN instrument?

FAQ ID -1329

View

How can I ensure that reaction volume is not lost due to evaporation during thermal cycling?

FAQ ID -2679

View

How can I estimate the size of my protein based on the length of the respective cDNA?

FAQ ID -2952

View

How can I express toxic protein in E. coli?

FAQ ID -373

View

How can I extract DNA from a polyacrylamide (PAGE) gel?

FAQ ID -120

View

How can I extract proteins from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?

FAQ ID -3049

View

How can I find out if my gene of interest is expressed in a specific tissue type or cell line?

FAQ ID - 3214

View

How can I find the run parameters for a BioSprint protocol that I am running?

FAQ ID -3069

View

How can I get a certificate for quality assurance (ISO 13485 and ISO 9001) from QIAGEN?

FAQ ID -762

View

How can I get software updates for the QIAcube Connect MDx?

148734

View

How can I get software updates for the QIAcube Connect?

FAQ ID - 141494

View

How can I get software updates for the QIAcube?

FAQ ID -1413

View

How can I get specific information and a quote for my individual Sequencing Service needs?

FAQ ID -792

View

How can I identify the binding sites of my transcription factor using ChIP?

FAQ ID -2753

View

How can I improve DNA yields from very tough tissues using the DNeasy Blood & Tissue Kit or the QIAamp DNA Mini Kit?

FAQ ID -374

View

How can I improve ligation efficiency of DNA from a QIAEX II Gel Extraction Kit?

FAQ ID -141

View

How can I improve my RNA yield from liver sample when using RNeasy Kits?

FAQ ID -623

View

How can I improve recoveries when using the QIAquick Kits?

FAQ ID -180

View

How can I improve the expression of proteins containing hydrophobic regions?

FAQ ID -339

View

How can I improve the performance of the HiSpeed QIAprecipitator module?

FAQ ID -144

View

How can I improve transfection efficiency using Effectene Transfection Reagent?

FAQ ID -181

View

How can I increase DNA concentration using QIAprecipitators of the HiSpeed Plasmid Kits?

FAQ ID -1061

View

How can I increase expression of my 6xHis-tagged protein in E. coli?

FAQ ID -63

View

How can I increase low-copy plasmid DNA yields using QIAfilter Plasmid Kits?

FAQ ID -1062

View

How can I increase the amount of soluble recombinant protein in E. coli expression?

FAQ ID -64

View

How can I increase the DNA yield from low-copy plasmids using the QuickLyse Miniprep Kit?

FAQ ID -1354

View

How can I isolate RNA from 1 gram of plant tissue?

FAQ ID -128

View

How can I keep my centrifuge tubes endotoxin-free?

FAQ ID -301

View

How can I know whether there is any genomic DNA present in my purified plasmid?

FAQ ID -3134

View

How can I load new protocols onto the QIAcube Connect MDx?

148740

View

How can I load new protocols onto the QIAcube Connect?

FAQ ID - 141501

View

How can I load new protocols onto the QIAcube?

FAQ ID -1421

View

How can I minimize degradation of RNA from my pancreas sample?

FAQ ID -624

View

How can I normalize my EpiTect ChIP results?

FAQ ID -2751

View

How can I obtain DNA-free RNA using an RNeasy Midi or Maxi Kit?

FAQ ID -143

View

How can I optimize Mg2+ conditions for a specific amplicon when using Phoenix Hot Start Taq and the supplied reaction buffer?

FAQ ID - 3902

View

How can I optimize Mg2+ conditions for a specific amplicon when using VeraSeq 2.0 DNA Polymerase and the supplied reaction buffers?

FAQ ID - 3909

View

How can I optimize Mg2+ conditions for a specific amplicon when using VeraSeq ULtra DNA Polymerase and the supplied reaction buffers?

FAQ ID - 3922

View

How can I optimize the transfection of oligos and large plasmids using PolyFect Transfection Reagent?

FAQ ID -176

View

How can I precipitate genomic DNA using isopropanol?

FAQ ID -2953

View

How can I predict the percent qPCR signal due to contaminating DNA, for a given qPCR assay, and its matching NRT control?

FAQ ID -2688

View

How can I prevent clogging of QIAfilter cartridges?

FAQ ID -1060

View

How can I prevent non-specific binding when using BioMag?

FAQ ID -277

View

How can I prevent running out of M48 Kit reagents before the full number of samples are processed?

FAQ ID -1042

View

How can I prevent the evaporation of reaction volume from the wells EpiTect Methyl qPCR Arrays?

FAQ ID -2742

View

How can I prevent the evaporation of reaction volume from the wells in the miScript miRNA PCR array?

FAQ ID -2729

View

How can I purify DNA from soil, food and sewage samples for PCR?

FAQ ID -118

View

How can I purify mRNA from total RNA using an Oligotex Direct mRNA Kit?

FAQ ID -201

View

How can I purify very small amounts of 6xHis-tagged protein using Ni-NTA technology?

FAQ ID -134

View

How can I quantify the amount of REPLI-g DNA I have amplified?

FAQ ID -694

View

How can I remove imidazole from a protein sample?

FAQ ID -91

View

How can I resuspend the lyophilized assay?

FAQ ID - 3864

View

How can I separate PCR fragments that are small and very close in size on an agarose gel?

FAQ ID -800

View

How can I streamline the quantification workflow?

FAQ ID - 3738

View

How can I tell if I have primer-dimers in my PCR reaction?

FAQ ID -552

View

How can I tell TurboCapture Microplate Holder Type A from Type B?

FAQ ID -1039

View

How can I transfect siRNA into insect cells such as Drosophila melanogaster S2?

FAQ ID -1046

View

How can I upload my gene list for FlexiPlate siRNA to GeneGlobe?

FAQ ID -1364

View

How can I use the SureSilencing shRNA Plasmids for stable transfection and knock down in a cell line that is already resistant to both neomycin and puromycin?

FAQ ID -2895

View

How can one determine the optimal annealing temperature for a specific PCR assay?

FAQ ID -288

View

How can one perform multiplex real-time PCR analysis using the LightCycler 2.0?

FAQ ID -780

View

How can PCR cycling conditions be optimized for Phoenix Hot Start Taq?

FAQ ID - 3899

View

How can proteins purified from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue be analyzed?

FAQ ID -3052

View

How can QIAGEN Protease and Proteinase K be inactivated?

FAQ ID -315

View

How can RNA interference be used as a life science research tool?

FAQ ID -2756

View

How can samples be shipped and stored prior to extraction on the Autopure LS?

FAQ ID - 3522

View

How can samples be shipped and stored prior to extraction on the Autopure LS?

FAQ ID - 3528

View

How can the QIAsymphony SP system be decontaminated?

FAQ ID -1914

View

How can we get our BioRobot EZ1 upgraded when new protocols are launched?

FAQ ID -1241

View

How can yield for long targets be increased when using Phoenix Hot Start Taq?

FAQ ID - 3907

View

How can yield for targets be increased when using VeraSeq 2.0 DNA Polymerase?

FAQ ID - 3914

View

How can yield for targets be increased when using VeraSeq ULtra DNA Polymerase?

FAQ ID - 3926

View

How comparable is CoralLoad gel loading dye contained in various QIAGEN PCR Kits to Sigma Red?

FAQ ID -1644

View

How complete is the removal of DAPase in the TAGZyme process?

FAQ ID -319

View

How critical is the time of the temperature change from 60°C to 80°C?

FAQ ID - 143768

View

How did you calculate the conversion factor?

FAQ ID - 3937

View

How did you determine that your AllStar Negative Control siRNA binds to RISC?

FAQ ID -1180

View

How do I analyze codon 61 mutations with the RAS extension kit (cat. no. 971590)?

FAQ ID - 3622

View

How do I analyze the data for the particular iPSC qBiomarker Arrays I have ordered?

FAQ ID -2440

View

How do I calculate the percentage of silencing with real-time RT-PCR for siRNA?

FAQ ID -498

View

How do I choose between the DNA-based Cignal Reporter Assays and the Cignal Lenti Reporter Assays?

FAQ ID -2761

View

How do I choose EpiTect ChIP-grade antibodies?

FAQ ID -2746

View

How do I clean up RNA preparations containing miRNA?

FAQ ID -3002

View

How do I create a workspace that is free of DNA contamination, prior to carrying out a qPCR experiment?

FAQ ID -2654

View

How do I determine if my gene expression results are statistically significant in the REST software?

FAQ ID -2458

View

How do I determine the amplification efficiency of my qPCR assay?

FAQ ID -2694

View

How do I determine the linear dynamic range of my qPCR or qRT-PCR assay?

FAQ ID -2696

View

How do I find the SureSilencing shRNA Plasmids for my gene of interest?

FAQ ID -2883

View

How do I get started using the NeXtal CubicPhase kits?

FAQ ID -2413

View

How do I harvest the crystals from meso phase? Are special tools needed with the NexTal Cubic Phase products?

FAQ ID -2330

View

How do I harvest the crystals from meso phase? Are special tools needed?

FAQ ID -2422

View

How do I insert and replace an absorber strip into the PyroMark Q48 Autoprep chamber?

FAQ ID - 3691

View

How do I know if my plasmid is a high- or low copy number type?

FAQ ID -350

View

How do I know if the assays are compatible with the sample DNA of interest?

FAQ ID - 3870

View

How do I know if the CGT dPCR assays are compatible with the sample DNA of interest?

FAQ ID - 3980

View

How do I know in which phase of my NeXtal CubicPhase cristallisation experiment I am?

FAQ ID -2421

View

How do I know whether my miRNA has been successfully purified and how much is expected?

FAQ ID -3135

View

How do I obtain the concentration of my gDNA from the data analysis using the GeneRead DNA QuantiMIZE?

FAQ ID - 3530

View

How do I perform a DNA precipitation to concentrate my sample?

FAQ ID -305

View

How do I perform an Acetone Precipitation for concentrating and desalting protein samples?

FAQ ID -1035

View

How do I perform the PyroMark Q24 Advanced upgrade?

FAQ ID -3163

View

How do I prepare an insert for pQE vectors?

FAQ ID -185

View

How do I prepare Buffer MP?

FAQ ID - 3620

View

How do I prevent a drifting baseline in my pyrosequencing pyrogram? If this method cannot be selected automatically in the application software, you can download the method/code file from the instrument webpage.

FAQ ID -2878

View

How do I prevent bubbles from forming in my Ni-NTA agarose column?

FAQ ID -285

View

How do I proceed if the amount of starting material cannot be calculated in detail?

FAQ ID - 3943

View

How do I reduce background peaks in the pyrosequencing pyrogram?

FAQ ID -2877

View

How do I remove the RNA contamination from purified DNA?

FAQ ID -3133

View

How do I resuspend my FlexiPlate siRNAs?

FAQ ID -1360

View

How do I safely inactivate biohazardous flow-through material?

FAQ ID -12

View

How do I search for all the miScript Target Protectors for one species?

FAQ ID -2255

View

How do I set up a PyroMark CpG Assay?

FAQ ID -2814

View

How do I store my FlexiTube siRNA Premix?

FAQ ID -2270

View

How do I submit a siRNA order by telephone or online?

FAQ ID -399

View

How do I use EasyXtal Crystallization Tools?

FAQ ID -1105

View

How do I use the qBiomarker Somatic Mutation PCR Arrays?

FAQ ID -2923

View

How do QuantiFast PCR Kits compare to QuantiTect PCR Kits for quantitative real-time PCR?

FAQ ID -1441

View

How do Rotor-Gene Probe Kits compare with QuantiFast Probe Kits?

FAQ ID -2125

View

How do the FastLane Cell cDNA Kit and QuantiTect Reverse Transcription Kit eliminate genomic DNA contamination?

FAQ ID -783

View

How do Unique Molecular Indexes (UMIs) improve quantification?

FAQ ID - 3669

View

How do you achieve fast cycling, yet still deliver the same performance in PCR as that achieved with standard cycling?

FAQ ID -1430

View

How do you choose which assays to include on the arrays?

FAQ ID — 3420

View

How do you decide on the qBiomarker Somatic Mutation PCR Arrays to include on the pathway arrays and disease arrays?

FAQ ID -2913

View

How do you design your EpiTect ChIP qPCR Assay primers? Do they work with SB GR?

FAQ ID -2749

View

How do you determine the efficiency using the PCR array?

FAQ ID -2701

View

How do you ensure that concentrated RNA is purified from saliva using the RNeasy Protect Saliva Mini Kit?

FAQ ID -1212

View

How do you ensure that RNeasy buffers are RNase-free?

FAQ ID -113

View

How does a Service Support Agreement provide defined budgeting for maintaining my BioRobot?

FAQ ID -1330

View

How does HotStart PCR help minimize nonspecific amplification events?

FAQ ID -2676

View

How does imidazole affect my quantitation of protein?

FAQ ID -132

View

How does magnetic-particle carry-over in QIAsymphony eluates need to be handled?

FAQ ID -2166

View

How does QIAGEN control the quality of the RT² qPCR Primer Assays?

FAQ ID -2711

View

How does QIAseq FastSelect work?

FAQ ID -147907

View

How does QIAsymphony SP keep cellular samples in suspension prior to pipetting so that samples do not settle?

FAQ ID -1905

View

How does stable transfection of a shRNA-encoding plasmid work?

FAQ ID -2779

View

How does template quality influence the results with the Type-it HRM PCR Kit?

FAQ ID -2197

View

How does the built-in QC control for complete bisulfite conversion of DNA work on PyroMark Q24?

FAQ ID -2095

View

How does the DNA Protection work in the EpitTect Bisulfite kits?

FAQ ID -2410

View

How does the gDNA eliminator solution of the RNeasy Plus Universal Kit work?

FAQ ID -2341

View

How does the liquid level detection work and what is the minimal detectable volume for the conductive tips of the BioRobot Universal?

FAQ ID - 3524

View

How does the QuantiFast Probe RT-PCR Plus Kit eliminate genomic DNA contamination?

FAQ ID -2354

View

How does the RNeasy Microarray Tissue Mini Kit prevent traces of phenol from negatively affecting the reverse-transcription step in microarray experiments?

FAQ ID -2189

View

How does the sealing efficiency of the crystallization supports of the EasyXtal 15-Well Tool compare to grease?

FAQ ID -1109

View

How does the stabilizing reagent in the PAXgene Saliva Collector stabilize saliva?

3814

View

How easy is it to scoop up a protein crystal with the lip around the crystallization supports of the EasyXtal 15-Well Tools?

FAQ ID -1113

View

How efficient is DNA removal by a gDNA Eliminator 96 plate compared with DNase digestion on an RNeasy 96 plate?

FAQ ID -1994

View

How fast does the rotor spin during a run on the Rotor-Gene Q?

FAQ ID -2080

View

How fast is the 6xHis-tagged protein purification process using Ni-NTA Superflow Cartridges?

FAQ ID -1605

View

How great is the enrichment of miRNA when using the dedicated PAXgene Blood miRNA Kit compared to the PAXgene Blood RNA Kit?

FAQ ID -2495

View

How important is the RNA purification process, for obtaining reliable qRT-PCR results?

FAQ ID -2655

View

How is "Touchdown PCR" used to increase PCR specificity?

FAQ ID -75

View

How is an antibody epitope determined?

FAQ ID -593

View

How is ARMS technology superior to alternative methods such as dye terminator sequencing?

FAQ ID -2290

View

How is stability and performance of these mericon Assay kits guaranteed?

FAQ ID -2542

View

How is the hypothesis test performed in the REST software?

FAQ ID -2459

View

How is the log graph threshold value (Ct) for the Rotor-Gene Q determined?

FAQ ID -2088

View

How is the Pyromark Q48 cartridge to be cleaned?

FAQ ID - 3718

View

How is the quality of proteins purified from PAXgene Tissue fixed and stabilized tissue?

FAQ ID - 3619

View

How is the quality of the crystallization condition Screening Suites guaranteed?

FAQ ID -1126

View

How is the RNeasy Lipid Tissue Mini Kit different from the RNeasy Mini Kit?

FAQ ID -468

View

How is the RNeasy Microarray Tissue Mini Kit different from the classic RNeasy Mini Kit?

FAQ ID -2192

View

How is the RNeasy Plus Universal Kit different from the RNeasy Plus Kit?

FAQ ID -2340

View

How is total RNA purified with RNeasy kits free of genomic DNA contamination?

FAQ ID -3154

View

How is VeraSeq 2.0 DNA Polymerase different from the standard recombinant Taq-B DNA Polymerase?

FAQ ID - 3908

View

How is VeraSeq ULtra DNA Polymerase different from the standard recombinant Taq-B DNA Polymerase?

FAQ ID - 3921

View

How long after first use can a reagent cartridge be stored for further use?

FAQ ID - 3650

View

How long and at which temperature can plasma generated from whole blood in PAXgene Blood ccfDNA Tubes be stored?

FAQ ID - 3644

View

How long are sample lysates stable in the fridge/ freezer?

FAQ ID - 143765

View

How long can a reagent cartridge remain open without affecting my results?

FAQ ID - 3706

View

How long can a tissue specimen stay in the PAXgene Tissue STABILIZER?

FAQ ID -3028

View

How long can DNA converted with EpiTect Bisulfite Kits be stored before use in methylation specific PCR with the EpiTect MSP Kit?

FAQ ID -2002

View

How long can eluates be stored after sample preparation using the EZ1 instruments or EZ2 Connect MDx and the EZ1 DSP Virus Kit?

FAQ ID - 3897

View

How long can homogenates remain on the QIAsymphony SP without risk of RNA degradation?

FAQ ID -1941

View

How long can I archive cDNA generated with the QuantiTect Whole Transcriptome Kit?

FAQ ID -1623

View

How long can I store an RLT lysate?

FAQ ID -3115

View

How long can I store bacterial culture stabilized in RNAprotect Bacteria Reagent?

FAQ ID -614

View

How long can I store my purified DNA/RNA isolated with the AllPrep DNA/RNA FFPE kit?

FAQ ID -2350

View

How long can I store the dilutions of the Control DNA Z1 standards at 2°C?

-8

View

How long can I store the dilutions of the Male Control DNA M1 standards at 2–8°C?

FAQ ID - 3744

View

How long can I store the reconsituted assay regarding QIAcuity HEK293 resDNA Quant Kit (96) at 4°C?

FAQ ID - 3934

View

How long can I store the reconstituted Internal Control and the reconstituted Positive Control?

FAQ ID - 3997

View

How long can primer and probes from the mericon Assay kits be kept at°C once reconstituted?

-20

View

How long can reaction components incubate with VeraSeq 2.0 DNA Polymerase at room temperature prior to PCR cycling?

FAQ ID - 3917

View

How long can saliva in PAXgene Saliva Collector tubes be stored at room temperature?

3815

View

How long can samples in PAXgene Blood DNA Tubes be stored at room temperature?

FAQ ID - 3508

View

How long can the PAXgene Blood DNA Tube be kept at room temperature? What is room temperature?

FAQ ID - 3492

View

How long can the PAXgene Blood RNA tubes be kept frozen at –20°C or –70°C?

FAQ ID - 3463

View

How long can whole blood stabilized in PAXgene Blood ccfDNA Tubes be stored at room temperature?

FAQ ID - 3639

View

How long does a QuantiFast Pathogen + IC run take on the Rotor-Gene Q?

FAQ ID -2452

View

How long does a run take?

FAQ ID -2566

View

How long does a saliva collection take?

3804

View

How long does a single run on the PyroMark Q24 take?

FAQ ID -2096

View

How long does a typical in vitro translation reaction take with your large-scale EasyXpress protein synthesis kits?

FAQ ID -880

View

How long does it take to generate results using the KRAS and EGFR PCR kits?

FAQ ID -2299

View

How long does it take to set up a 96-well plate PCR reaction on the QIAgility?

FAQ ID -3066

View

How long does the sample disruption with the TissueLyser LT take?

FAQ ID -2550

View

How long is cDNA generated with the QuantiTect Whole Transcriptome Kit?

FAQ ID -1613

View

How long is fluorescence detectable in cells after transfection with fluorescently labeled siRNAs?

FAQ ID -392

View

How long is the fixation time when using PAXgene Tissue FIX Container?

FAQ ID -3026

View

How long is the fixation time?

FAQ ID -2519

View

How long should I wait after transfection before enriching my transfected cells, validating knock down, and looking for phenotypic changes?

FAQ ID -2893

View

How long should PCR fragments be for the SeqTarget System?

FAQ ID -2236

View

How long should the amplicon be if I am using QuantiTect SYBR Green detection?

FAQ ID -553

View

How long will the intensity calibration procedure on the QIAxcel System take?

FAQ ID -1826

View

How magnetically responsive is BioMag?

FAQ ID -265

View

How many AAV genome copies can I load into the QIAcuity?

FAQ ID - 3977

View

How many assays can be multiplexed?

FAQ ID - 3862

View

How many cells can be used with the FastLane Cell cDNA Kit?

FAQ ID -796

View

How many cells can be used with the Qproteome Mitochondria Isolation Kit, and how much mitochondrial protein can be expected?

FAQ ID -1189

View

How many CpG sites are analyzed by the PyroMark CpG LINE assay?

2858

View

How many different genes and gene mutations are on a Somatic Mutation PCR Array?

FAQ ID -2427

View

How many drops can be set up on a protein crystallization support?

FAQ ID -1110

View

How many elution steps are recommended when using Glutathione Superflow resin?

FAQ ID -2175

View

How many experiments can I do with the Cignal Lenti Reporter Assays?

FAQ ID -2769

View

How many extractions can I perform with a QIAsymphony PAXgene Blood ccfDNA Kit, using the 2.4 ml or the 4.8 ml protocols?

FAQ ID - 3649

View

How many freeze-thaw cycles can the Internal Control supplied with Certal Residual DNA Detection Kits tolerate?

FAQ ID -2975

View

How many freeze-thaw cycles can the master mix contained in the QuantiFast Probe RT-PCR Plus Kit tolerate?

FAQ ID -2357

View

How many freeze-thaw cycles can the various reaction mixes in the EasyXpress Insect Kit II tolerate?

FAQ ID -1219

View

How many housekeeping genes are included in each PCR Array?

FAQ ID -2704

View

How many LiquiChip beads are recommended for an xMAP experiment?

FAQ ID -1161

View

How many miScript Precursor Assays do you offer?

FAQ ID -2213

View

How many molecules can be detected in the system?

153926

View

How many mutations can I profile per sample on the qBiomarker Somatic Mutation PCR Array?

FAQ ID -2911

View

How many nucleotides of a homopolymer can be resolved in pyrosequencing?

FAQ ID -2871

View

How many PAXgene Blood RNA Tubes can be processed per PAXgene 96 Blood RNA Kit well plate?

FAQ ID -2485

View

How many PCR reactions can be performed for different scales of miScript Primer Assays?

FAQ ID -1990

View

How many protein crystallization conditions are available at QIAGEN?

FAQ ID -1119

View

How many reactions can be performed per well of the QuantiTect Primer Assay Plates?

FAQ ID -2107

View

How many reactions can be performed with one QIAsymphony mericon Bacteria Kit?

FAQ ID -2955

View

How many reactions can I perform with the new QuantiFast Kits for real-time PCR?

FAQ ID -1425

View

How many reporter dyes can be used in the same analysis using the LiquiChip Workstation?

FAQ ID -738

View

How many samples can be processed per kit or per reagent cartridge for RNA purification on the QIAsymphony SP?

FAQ ID -1940

View

How many samples can be processed per RNeasy Mini QIAcube kit?

FAQ ID -2332

View

How many samples can be processed with the PAXgene Blood miRNA Kit?

FAQ ID - 3477

View

How many samples can be processed with the PAXgene Blood RNA MDx Kit?

FAQ ID -2490

View

How many samples can be processed with the QIAsymphony DNA Mini Kit and the Virus Blood 200 protocol?

FAQ ID -2159

View

How many samples can be processed with the QIAsymphony DSP Virus/Pathogen Kits?

FAQ ID -2935

View

How many samples can be processed with the QIAsymphony PAXgene Blood RNA Kit?

FAQ ID -2497

View

How many samples can be run in parallel with the Rotor-Gene Q real-time PCR instrument?

FAQ ID -1519

View

How many signal readings are taken during the real-time data acquisition on the Rotor-Gene Q?

FAQ ID -2083

View

How many template copies can I detect using QuantiTect Kits in real-time PCR?

FAQ ID -543

View

How many times can the cartridges for PyroMark Q24 or PyroMark Q96 ID instruments be reused?

FAQ ID -2863

View

How many times can the CDTs, NDTs, and RDTs be used?

FAQ ID -2865

View

How many times can the Internal Controls used in the QuantiFast Pathogen PCR +IC Kit and the QuantiFast Pathogen RT-PCR +IC Kit be freeze-thawed?

FAQ ID -2599

View

How many times can vacuum troughs be reused with the PyroMark Vacuum Preparation Stations?

FAQ ID -2848

View

How many transfections can be performed with Flexiplate siRNAs?

FAQ ID -3105

View

How many transfections can I perform with 20 nmol of HPP grade siRNA?

FAQ ID -367

View

How much active protein can be expressed in-vitro using the EasyXpress Insect Kit II, and how much time does it take?

FAQ ID -1222

View

How much buffer should be used for agarose gel electrophoresis?

FAQ ID -2257

View

How much can the mixed standard ?Ct values vary in the KRAS PCR kit?

FAQ ID -2307

View

How much cDNA is sufficient for real-time PCR detection of miRNA transcripts generated with the miScript System?

FAQ ID -1598

View

How much circulating cell-free DNA can I expect from samples, and how can I quantify the yield?

FAQ ID - 3704

View

How much detection reagent do you generally recommend for LiquiChip Assays?

FAQ ID -1159

View

How much DNA and RNA can be expected from human blood cells?

FAQ ID -2950

View

How much DNA does a bacterial cell contain?

FAQ ID -2947

View

How much DNA does a human cell contain?

FAQ ID -2948

View

How much DNA is needed in the PyroMark Q48?

FAQ ID - 3721

View

How much DNA is obtained in the average PCR reaction?

FAQ ID -750

View

How much DNA should be loaded per well of an agarose gel?

FAQ ID -2259

View

How much DNA should I use in a qBiomarker Copy Number PCR Assay?

FAQ ID — 3418

View

How much DNA should I use on a qBiomarker Copy Number PCR Array?

FAQ ID — 3417

View

How much DNA starting sample is needed for analysis with a Somatic Mutations PCR Array? And Assay?

FAQ ID -2431

View

How much DNA/RNA can be isolated with the AllPrep DNA/RNA FFPE Kit?

FAQ ID -2353

View

How much dye-labeled siRNA should I use to monitor transfection efficiency when using HiPerfect Transfection Reagent?

FAQ ID -1058

View

How much protein can be produced with the EasyXpress Protein Synthesis Mega Kit?

FAQ ID -843

View

How much protein does one vial of Protease in the QIAamp spin kits contain? What is the concentration of the resuspended Protease?

FAQ ID - 3520

View

How much REPLI-g amplified mitochondrial DNA should be used in a subsequent PCR reaction?

FAQ ID -1320

View

How much RNA does a bacterial cell contain?

FAQ ID -2949

View

How much RNA does a typical mammalian cell contain?

FAQ ID -2946

View

How much sample can be used with the Allprep DNA/RNA 96 Kit?

FAQ ID -1997

View

How much sample can be used with the RNeasy Plus 96 Kit?

FAQ ID -1992

View

How much sample can I load for detecting host cell contamination?

FAQ ID - 3879

View

How much sample is required for each well for an ELISArray Kit?

FAQ ID -2908

View

How much sample material can I use for DNA/RNA purification with the AllPrep DNA/RNA FFPE Kit?

FAQ ID -2351

View

How much solution can I add to an EasyXtal 15-Well Tool reservoir well?

FAQ ID -1114

View

How much space does the PyroMark Q24 instrument need?

FAQ ID -2098

View

How much space does the PyroMark Q48 Autoprep instrument need?

FAQ ID - 3693

View

How much starting material can be used with the QIAsymphony DNA Mini Kit on the QIAsymphony SP?

FAQ ID -1917

View

How much starting material can be used with the QIAsymphony DSP DNA Mini Kit on the QIAsymphony SP?

FAQ ID -2927

View

How much template can I use in the reaction and what is the maximum volume of template that can be used in the QuantiTect Kits?

FAQ ID -1086

View

How much time can be saved when using the RNeasy Mini QIAcube Kit?

FAQ ID -2335

View

How much time is needed for a LiquiChip assay point measurement?

FAQ ID -1162

View

How much time may the aspirated bone marrow sample be allowed to incubate before pipetting it into the PAXgene Bone Marrow RNA Tube?

FAQ ID -2504

View

How much time will be saved when switching from standard cycling to fast cycling with QuantiFast Kits?

FAQ ID -1438

View

How often can the Strep-Tactin Superflow Plus resin be reused?

FAQ ID -2169

View

How often should the alignment marker for the QIAxcel System be changed?

FAQ ID -1839

View

How often should the o-ring for the pipettor tip adapter be changed on the QIAcube Connect?

FAQ ID - 141509

View

How often should the solutions in the Buffer Tray for the QIAxcel System or QIAxcel Advanced be replaced?

FAQ ID -1840

View

How precise is the QIAgility?

FAQ ID -3063

View

How pure are the CTCs enriched from blood samples using the AdnaTest?

3831

View

How pure should my protein be with the NexTal CubicPhase kits?

FAQ ID -2414

View

How pure should the chloroform used with the RNeasy Microarray Tissue Mini Kit be?

FAQ ID -2193

View

How pure should the chloroform used with the RNeasy Plus Universal Mini Kit be?

FAQ ID -2344

View

How reliable are the results from the miScript Primer Assays?

FAQ ID -2730

View

How sensitive is the LiquiChip Reader?

FAQ ID -1157

View

How should blood samples drawn into PAXgene Blood DNA Tubes be shipped?

FAQ ID - 3489

View

How should blood samples drawn into PAXgene Blood DNA Tubes be transported?

FAQ ID - 3507

View

How should blood samples drawn into the PAXgene Blood DNA Tubes be stored?

FAQ ID - 3490

View

How should data be analyzed when the miScript Single Cell qPCR Kit is used with miScript miRNA PCR Arrays or miScript Primer Assays?

FAQ ID - 3581

View

How should DNA isolated on the QIAsymphony be stored?

FAQ ID -2165

View

How should DNA purified using the PAXgene Blood DNA System be stored?

FAQ ID - 3502

View

How should eluates produced with the PAXgene Blood ccfDNA System be stored?

FAQ ID - 3657

View

How should fluorescent labeled probes be stored?

FAQ ID -784

View

How should gels be cast with the GelPilot Agarose so that optimal resolution is achieved?

FAQ ID -2258

View

How should I analyze the data generated from a qBiomarker Copy Number PCR Array experiment?

FAQ ID — 3421

View

How should I dissolve the lyophilized Anti-His and Tag Antibodies?

-100

View

How should I prepare buffy coat samples for use on the BioRobot EZ1?

FAQ ID -1249

View

How should I propagate pQE expression plasmids?

FAQ ID -58

View

How should I quantify RNA isolated with RNeasy Kits?

FAQ ID -32

View

How should I reconstitute the lyophilized RNase-Free DNase Set with the RNeasy 96 kit?

FAQ ID -3003

View

How should I store Anti·His and Tag·100 Antibodies?

FAQ ID -194

View

How should I store cDNA produced using Omniscript Reverse Transcriptase?

FAQ ID -561

View

How should I store plant material for DNA isolation using the DNeasy Plant Kit?

FAQ ID -114

View

How should I store the REPLI-g amplification product?

FAQ ID -692

View

How should I thaw frozen PAXgene Blood DNA Tubes?

FAQ ID - 3495

View

How should I thaw frozen plasma samples?

FAQ ID - 3645

View

How should QIAGEN Plasmid Purification Kits be stored and for how long?

FAQ ID -192

View

How should RNeasy Kits be stored and how long are they stable?

FAQ ID -103

View

How should samples be stored before purification using the Virus Blood 200 Protocol on the QIAsymphony?

FAQ ID -2157

View

How should the GelPilot DNA Loading Dye be handled?

FAQ ID -1287

View

How should the PAXgene Bone Marrow RNA Tubes be frozen after collection and for how long?

FAQ ID -2505

View

How should the samples be stored before purification of RNA with the QIAsymphony PAXgene Blood RNA Protocol?

FAQ ID -2984

View

How should the samples be stored before purification with the QIAsymphony DSP Virus/Pathogen Kits?

FAQ ID -2934

View

How should unused PAXgene Blood ccfDNA Tubes be stored?

FAQ ID - 3634

View

How should unused PAXgene Saliva Collector be stored?

3812

View

How stable is Phoenix Hot Start Taq when incubated in a PCR reaction mix at room temperature?

FAQ ID - 3898

View

How to prepare DNA prior to dPCR?

3778

View

How to quantitate DNA purified using the BioSprint 96?

FAQ ID -773

View

How to store DNA from forensic samples eluted into water on the EZ1 biorobot in TE buffer instead?

FAQ ID -734

View

How to upload a Flexiplate on QIAGEN Webpage?

FAQ ID -3103

View

How was it determined that the PhosphoSerine Antibody will bind phosphoserines only?

FAQ ID -579

View

How well is DNA integrity preserved in PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?

FAQ ID - 3612

View

How well is RNA integrity preserved in PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?

FAQ ID - 3611

View

How will running less than 96 samples with the MagAttract 96 cador Pathogen kit affect usage of plasticware?

FAQ ID -2071

View

How will service work carried out on my QIAGEN BioRobot be documented?

FAQ ID -1331

View

How would I provide information on the QuantiTect Primer assays in publications when the sequence is confidential?

FAQ ID - 3593

View

I accidentally added RNAprotect Tissue Reagent to my cells, and now the cells are difficult to pellet. What can I do?

FAQ ID -364

View

I accidentally stored Buffer RDD of the RNase-Free DNase Set at°C. Will it still function?

-20

View

I am seeing a precipitate after adding LyseBlue reagent to Buffer P1. What should I do about that?

FAQ ID -1045

View

I am using an RNA library prep kit that is not listed in the QIAseq FastSelect handbook. Will QIAseq FastSelect work with my kit?

FAQ ID -147913

View

I bound an 11 kb DNA fragment to a QIAquick column; is it completely lost?

FAQ ID -756

View

I cannot analyze the data from your Investigator HID kits with my GeneMapper software. What can I do?

FAQ ID - 3363

View

I cannot see the LCD display fonts on the Sigma Centrifuge. How do I adjust the contrast?

FAQ ID -822

View

I do not see any miRNA expression for my miRNAs of interest after performing single cell miRNA expression analysis with the miScript Single Cell qPCR Kit and miScript miRNA PCR Arrays or miScript Primer Assays. What could be the cause?

FAQ ID - 3577

View

I don’t have any glassware in the lab, is the QIAwave kit still a good option for me?

FAQ ID - 3989

View

I have a Human Druggable Genome siRNA Set V2.0. Can I reorder the siRNAs from this set?

FAQ ID -1366

View

I have accidentally pipetted beads when removing the eluate after cDNA cleanup. What should I do?

FAQ ID - 3572

View

I left Buffer P1 at room temperature after addition of RNase A, what shall I do?

FAQ ID -859

View

I need to buy a new computer for my PyroMark Q96. Can I reinstall the PyroMark Q96 software on my new computer or do I need to purchase a new license?

FAQ ID - 3472

View

I ran my RNA out on an agarose gel and can see lots of bands similar to a ladder. Why?

FAQ ID -745

View

I received a kit containing the MinElute columns; however, they were left out for a while and not stored at 2–8°C upon receipt. Can I still use them?

FAQ ID - 3560

View

I routinely heat an aliquot of the RNA eluate prior to my RT reaction. Do I still need to heat the eluate after the PAXgene Bone Marrow RNA preparation?

FAQ ID -2513

View

I use cultured cells as starting material. Can I also use the RNeasy Plus Universal Kit?

FAQ ID -2342

View

I used to buy BioMag particles from a company by another name? Are your BioMag particles the same thing?

FAQ ID -263

View

I want to purify RNA from heart tissue. What is the best kit to use?

FAQ ID -3116

View

I want to start with gene silencing (RNAi) experiments. Do you have informational literature?

FAQ ID -580

View

I would like to order the QuantiFast Probe Assays without master mix. Is this possible?

FAQ ID -2360

View

I would like to sequence my siRNA constructs. Does QIAGEN offer Sequencing Services for cloned siRNA?

FAQ ID -829

View

I would like to stop during the library preparation procedure. When can I do that?

FAQ ID - 3687

View

I would like to use the kit on another third party instrument. Who should I contact?

3803

View

If 400 µl are used as lysis volume, how do I concentrate the lysate? (Otherwise the DNA would be too diluted.)

FAQ ID - 143766

View

If a cycler from Applied Biosystems is used in combination with the Certal Residual DNA Detection Kit, should dye calibration for MAX be performed?

FAQ ID -2977

View

If a QuantiTect Primer Assay has more than one version number, does this mean that earlier assay versions may not work?

FAQ ID -1136

View

If both MaXtract Low Density and High Density is suitable for an application, which type is preferable to use?

FAQ ID -1310

View

If FlexiPlate siRNA was accidentally redissolved in siRNA Suspension Buffer instead of water, resulting in a 2x buffer, would it affect performance of the siRNAs?

FAQ ID -1477

View

If housekeeping and target genes differ significantly in abundance, will they both be amplified with equal efficiency using the Rotor-Gene Multiplex PCR Kit?

FAQ ID -2129

View

If I am using BioMag Streptavidin to capture biotinylated oligos, how can I then remove the oligos?

FAQ ID -279

View

If I am working with a difficult-to-transfect cell type or if I obtain only weak silencing effects what can I do?

FAQ ID -2265

View

If I cannot find a QuantiTect Primer Assay for my target gene in rat, can I use the assays for the orthologous genes in mouse and human?

FAQ ID -1145

View

If I cannot order FlexiPlate siRNA online, how do I order?

FAQ ID -1371

View

If I want to use BioMag particles, do I have to buy a QIAGEN magnet too or can I use one that I already have?

FAQ ID -266

View

If less than 48/96 samples are being processed, is it possible to leave positions empty with the PAXgene Blood RNA MDx Kit?

FAQ ID -2492

View

If new dyes or chemistries appear on the real-time PCR market, will Rotor-Gene Q be capable of running those dyes?

FAQ ID -2087

View

If orders cannot be placed online, how can QIAgenes be ordered?

FAQ ID -2046

View

If pQE40 is used to express a fusion protein with DHFR, can the DHFR be cleaved afterwards?

FAQ ID -513

View

If template abundance differs significantly between housekeeping and target gene, are both amplified equally efficient with the QuantiTect Multiplex Kits?

FAQ ID -718

View

If the M500 method is used with the QIAxcel System, does a new DNA reference marker table have to be created?

FAQ ID -1856

View

If Vapor-Lock is used to overlay PCR reactions, which volume should be entered as reaction volume in the Rotor-Gene Q Software?

FAQ ID -2150

View

In the REST software, what is the range indicated by 'Std. Error' on the 'Results' page?

FAQ ID -2455

View

In the results table generated with the QIAxcel System, what does "normalized area" mean?

FAQ ID -1854

View

In which channels can you detect the CGT dPCR assays?

FAQ ID - 3863

View

In which format can PyroMark CpG Assays be ordered?

FAQ ID -2824

View

In which formats are crystallization condition Screening Suites available?

FAQ ID -1127

View

In which solution can I keep my protein for the NexTal Cubic Phase kits?

FAQ ID -2416

View

In which tubes is clot detection possible on QIAsymphony?

FAQ ID - 3589

View

Investigator Quantiplex and Quantiplex Pro: What do I have to consider if I am using Applied Biosystems SDS software version 1.2.3?

FAQ ID -2570

View

Investigator Quantiplex Pro RGQ: On which real-time cyclers is the kit validated?

FAQ ID - 3741

View

Investigator Quantiplex Pro RGQ: Which real-time cycler software version can be used to run the kit?

FAQ ID - 3743

View

Investigator Quantiplex Pro RGQ: Which Rotor-Gene Q System can be used to run the kit?

FAQ ID - 3742

View

Investigator Quantiplex Pro: Can the kit be run on the Rotor-Gene Q?

FAQ ID - 3729

View

Investigator Quantiplex Pro: Do I have to calibrate new dyes on my Applied Biosystems 7500 or QuantStudio 5 Real-Time PCR System?

FAQ ID - 3732

View

Investigator Quantiplex Pro: On which real-time cyclers is the Investigator Quantiplex Pro Kit validated?

FAQ ID - 3728

View

Investigator Quantiplex Pro: Which real-time cycler software version can be used to run the kit?

FAQ ID - 3730

View

Investigator Quantiplex: Do I have to calibrate new dyes on my Applied Biosystems 7500 or 7500 Fast Real-Time PCR System?

FAQ ID -2571

View

Investigator Quantiplex: For which real-time cyclers has the kit been validated?

FAQ ID -2567

View

Investigator Quantiplex: Which version of the Applied Biosystems 7500 Real-Time PCR System software can be used to run the Investigator Quantiplex?

FAQ ID -2569

View

Investigator Quantiplex: Which version of the Rotor Gene-Q software can be used to run the kit?

FAQ ID -2568

View

Is 2 kb the minimal gDNA fragment size for REPLI-g Whole Genome Amplification?

FAQ ID -682

View

Is a passive internal reference dye, like ROX, required on the QIAquant to obtain reproducible results?

153920

View

Is a passive internal reference dye, like ROX, required on the Rotor-Gene Q to obtain reproducible results?

FAQ ID -2079

View

Is a restriction digest necessary for DNA isolated from PAXgene Saliva samples before performing a dPCR run using the QIAcuity?

3829

View

Is a saliva collection device provided in the RNeasy Protect Saliva Mini Kit?

FAQ ID -1213

View

Is a special processing protocol needed?

FAQ ID -2523

View

Is a special processing protocol needed?

FAQ ID -3035

View

Is a standard curve needed in dPCR?

3786

View

Is an additional proteinase K digest possible with DNeasy PowerSoil Kit and DNeasy PowerLyzer Kit?

FAQ ID - 3725

View

Is cDNA generated with the QuantiTect Whole Transcriptome Kit suitable for use in microarray analysis?

FAQ ID -1587

View

Is clot detection possible on the QIAsymphony?

FAQ - ID 3588

View

Is dihydrofolate reductase (DHFR) immunogenic?

FAQ ID -471

View

Is DNA extraction from the samples needed?

FAQ ID - 3873

View

Is double centrifugation required for plasma processing?

FAQ ID - 3643

View

Is dPCR sensitive to inhibition?

FAQ ID - 3880

View

Is Factor SB of the HotStar HiFidelity Polymerase Kit the same as Factor MP of the QIAGEN Multiplex PCR Kit?

FAQ ID -1049

View

Is FlexiTube GeneSolution available for other scales than the 1 nmol scale?

FAQ ID -1666

View

Is food DNA co-purified using the QIAsymphony mericon Bacteria Kit?

FAQ ID -2959

View

Is GFP excited and detected by the green laser of the LiquiChip System?

FAQ ID -737

View

Is it acceptable to correct for transfection efficiency, when determining the level of gene expression knock down by an shRNA expression vector?

FAQ ID -2783

View

Is it also possible to isolate miRNA with the AllPrep DNA/RNA FFPE Kit?

FAQ ID -2348

View

Is it good to pool multiple RNA replicates to detect expression changes that are consistently reproducible?

FAQ ID -2663

View

Is it necessary to clean a processor normally used for formalin-fixed tissue before using it with PAXgene Tissue fixed tissue?

FAQ ID -3034

View

Is it necessary to clean a processor normally used for formalin-fixed tissue before using it with PAXgene Tissue fixed tissue?

FAQ ID - 3607

View

Is it necessary to clean up cDNA prepared with the QuantiTect Whole Transcriptome Kit?

FAQ ID -1610

View

Is it necessary to include a genomic DNA removal step with the QuantiTect Whole Transcriptome Kit?

FAQ ID -1614

View

Is it necessary to perform calibration steps for the use of the MAX dye in duplex RT-PCR experiments on different cyclers?

FAQ ID -2373

View

Is it necessary to place the lids of the elution tube and column into the slots of the rotor adapter during processing on the QIAcube Connect?

FAQ ID - 141496

View

Is it necessary to place the lids of the elution tube and column into the slots of the rotor adapter during processing on the QIAcube?

FAQ ID -1415

View

Is it necessary to place the lids of the elution tube and column into the slots of the rotor adapter during processing on the QIAcube?

148736

View

Is it necessary to reanalyze a plate with VPF (Volume Precision Factor) that was already processed using a QIAcuity instrument that was purchased in 2020?

3771

View

Is it normal that the binding capacity of GST-resins decreases with flow rate?

FAQ ID -2174

View

Is it possible to adjust the Lysis Buffer volume used with the Qproteome Bacterial Protein Preparation Kit?

FAQ ID -841

View

Is it possible to archive PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue blocks?

FAQ ID -2524

View

Is it possible to archive PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?

FAQ ID -3038

View

Is it possible to buy the EZ Competent Cells of the QIAGEN PCR Cloning Plus Kit separately?

FAQ ID -763

View

Is it possible to change voltage set-up from 110V to 230V on the QIAcuity instruments?

3761

View

Is it possible to clean up a methylation reaction containing bisulfite with QIAquick Cleanup Kits?

FAQ ID -519

View

Is it possible to cleave the 6xHis-tag from an expressed protein?

FAQ ID -140

View

Is it possible to collect intact mitochondria before or after the density gradient centrifugation step of the Qproteome Mitochondria Isolation protocol?

FAQ ID -1187

View

Is it possible to edit the sample sheet after the run in the QIAquant Software?

153955

View

Is it possible to edit the sample sheet in the Rotor-Gene Q software after a PCR run?

FAQ ID -2092

View

Is it possible to elute plasmid DNA from the QIAprep Spin Miniprep columns with buffer containing Potassium Phosphate?

FAQ ID -854

View

Is it possible to elute plasmid DNA obtained with the QuickLyse Miniprep Kit in nuclease-free water?

FAQ ID -1355

View

Is it possible to embed PAXgene Tissue fixed and stabilized samples in Optimal Cutting Temperature (OCT) medium for freezing?

FAQ ID -2525

View

Is it possible to establish an internet connection with QIAquant instrument?

153952

View

Is it possible to extract RNA from frozen blood?

FAQ ID -3119

View

Is it possible to extract RNA from RNAprotect-stabilized-blood?

FAQ ID -3120

View

Is it possible to fix over a weekend?

FAQ ID -3027

View

Is it possible to get a Custom RT2 Profiler PCR Array, cat. # 330131, with primer assays targeting transcripts of different species, e.g. assays targeting transcripts from mouse and assays targeting transcripts from human, on one plate?

FAQ ID - 3710

View

Is it possible to import a standard curve from a previous PCR run on the Rotor-Gene Q?

FAQ ID -2093

View

Is it possible to import a standard curve from a previous run?

153947

View

Is it possible to increase nucleic acid concentrations when following protocols on the BioRobot EZ1?

FAQ ID -1232

View

Is it possible to increase the amount of sample to obtain higher signals using the Investigator IDplex GO! Kit?

FAQ ID -3090

View

Is it possible to integrate the PAXgene Tissue STABILIZER into the processing?

FAQ ID -3037

View

Is it possible to integrate the PAXgene Tissue STABILIZER into tissue processing?

FAQ ID - 3608

View

Is it possible to interrupt the PAXgene Blood DNA kit protocol?

FAQ ID - 3503

View

Is it possible to introduce an affinity tag using the EasyXpress Linear Template Fab Kit?

FAQ ID -2972

View

Is it possible to isolate both RNA and recombinant 6xHis-tagged protein from the same sample?

FAQ ID -532

View

Is it possible to isolate DNA from bone marrow with the QIAamp DNA Blood Kits?

FAQ ID -563

View

Is it possible to isolate DNA from Saliva Stabilized in RNAprotect® Saliva Reagent?

FAQ ID -2995

View

Is it possible to isolate free genomic DNA using the QIAamp UltraSens Virus Kit?

FAQ ID -358

View

Is it possible to isolate genomic DNA or viral nucleic acids with PAXgene Blood miRNA protocol?

FAQ ID - 3483

View

Is it possible to isolate genomic DNA or viral nucleic acids with the QIAsymphony PAXgene Blood RNA protocol?

FAQ ID - 3476

View

Is it possible to isolate single stranded DNA (ssDNA) with the QIAEX II Kit from agarose or polyacrylamide gels?

FAQ ID -576

View

Is it possible to manually process PAXgene Tissue treated samples without using an automated processor?

FAQ ID -3036

View

Is it possible to measure the proteolytic activity of proteins on the surface of exosomes that were isolated with ExoEasy?

FAQ ID - 3599

View

Is it possible to microdissect PAXgene Tissue fixed, paraffin-embedded (PFPE) and PAXgene Tissue fixed, cryo-embedded (PFCE) tissues?

FAQ ID -2531

View

Is it possible to modify QIAsymphony protocols?

FAQ ID -1903

View

Is it possible to modify the transduction protocol for Cignal Lenti Reporter Assays to save some pipetting steps?

FAQ ID - 3709

View

Is it possible to normalize to more or less than 500 ng with the SeqTarget System if needed?

FAQ ID -2235

View

Is it possible to partially use RNeasy 96 plates and use the plate multiple times?

FAQ ID -3124

View

Is it possible to perform a UNG treatment when using QuantiTect kits?

FAQ ID -564

View

Is it possible to perform immunoprecipitation (IP) directly on protein fractions from the Qproteome Cell Compartment Kit?

FAQ ID -855

View

Is it possible to perform laser microdissection with tissues stabilized with RNAprotect Tissue reagent?

FAQ ID -608

View

Is it possible to place the PAXgene Saliva Collector tube directly on the QIAsymphony SP instrument sample rack?

3825

View

Is it possible to process formalin-fixed and PAXgene Tissue fixed samples together in one run?

FAQ ID -3033

View

Is it possible to process formalin-fixed and PAXgene Tissue fixed samples together in one run?

FAQ ID - 3606

View

Is it possible to process volumes of saliva from the PAXgene Saliva Collector other than the 400 or 1000 µl from the standard protocols for the QIAsymphony DNA Midi Kit using the QIAsymphony SP instrument?

3824

View

Is it possible to purchase Buffer VXL which is part of the QIAamp cador Pathogen Mini Kit, (Cat No.: 54104, 54106) separately?

FAQ ID - 3712

View

Is it possible to purify a GST-tagged protein under denaturing conditions using Glutathione Superflow?

FAQ ID -2172

View

Is it possible to purify and normalize long range PCRs produced with PCR kits from other vendors for the SeqTarget System?

FAQ ID -2243

View

Is it possible to purify DNA from the whole sample of a PAXgene Saliva Collector in one preparation?

3826

View

Is it possible to purify viral RNA using FastLane Cell RT-PCR Kits?

FAQ ID -1484

View

Is it possible to quantify protein content in RLT lysates?

FAQ ID -3155

View

Is it possible to run therascreen mutation or therascreen Pyro kits on PyroMark Q24 Advanced system?

FAQ ID -3161

View

Is it possible to scale up QuantiTect Reverse Transcription reactions to allow use of larger amounts of RNA?

FAQ ID -1063

View

Is it possible to scale up sample volume and reaction volume?

FAQ ID - 3884

View

Is it possible to simultaneously isolate DNA and RNA from Saliva Stabilized in RNAprotect® Saliva Reagent?

FAQ ID -2996

View

Is it possible to stop the DNeasy tissue protocol and store the tissue lysates after digesting in buffer ATL and Proteinase K?

FAQ ID - 3362

View

Is it possible to store nucleic acids extracted using the SpeedXtract Virus Kit?

FAQ ID - 3680

View

Is it possible to store TAGzyme enzymes at°C?

-80

View

Is it possible to store the lysates? How can the lysates be stored?

FAQ ID - 3973

View

Is it possible to synthesize membrane proteins using the EasyXpress Protein Synthesis Kit?

FAQ ID -602

View

Is it possible to test the efficiency of FastSelect rRNA removal by using a Bioanalyzer, etc.?

FAQ ID -147916

View

Is it possible to transfect DNA into insect cell lines such as Drosophila melanogaster S2?

FAQ ID -397

View

Is it possible to upgrade existing PyroMark Q24 instruments to PyroMark Q24 Advanced or do I have to purchase a new instrument?

FAQ ID -3162

View

Is it possible to use a QIAzol lysate for the isolation of RNA from tissue on the BioRobot EZ1?

FAQ ID -573

View

Is it possible to use a standard processor — the kind used routinely for formalin-fixed samples — for dehydration and paraffin infiltration of PAXgene Tissue treated samples?

FAQ ID -3032

View

Is it possible to use ABI instruments without ROX as a passive reference?

FAQ ID - 3889

View

Is it possible to use different sample types in one run with the MagAttract 96 cador Pathogen Kit?

FAQ ID -2072

View

Is it possible to use formalin-fixed, paraffin-embedded (FFPE) kits and protocols for extraction of biomolecules from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?

FAQ ID -3043

View

Is it possible to use formalin-fixed, paraffin-embedded (FFPE) kits and protocols to isolate biomolecules from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?

FAQ ID - 3610

View

Is it possible to use frozen cell pellets with the FastLane Cell cDNA Kit?

FAQ ID -835

View

Is it possible to use less than 1 mg of protein with the PhosphoProtein Purification Kit?

FAQ ID -599

View

Is it possible to use LiquiChip beads for FACS applications?

FAQ ID -733

View

Is it possible to use lower siRNA concentrations with FlexiTube siRNA Premix?

FAQ ID -2264

View

Is it possible to use Lyse&Spin baskets?

FAQ ID - 143762

View

Is it possible to use MaXtract for an organic extraction with a mixture of phenol and BCP (1 bromine chlorpropane)?

-3

View