How can I isolate RNA from 1 gram of plant tissue?

For large-scale RNA isolation from plants, 500 mg of tissue can be processed using the RNeasy Midi Kit, and 1 g using the RNeasy Maxi Kit. With optimization, it may be possible to use even larger amounts of starting material. Please refer to Appendix D of the RNeasy Midi/Maxi Handbook for guidelines for plant Midi- and Maxi preps.