How much dye-labeled siRNA should I use to monitor transfection efficiency when using HiPerfect Transfection Reagent?

The amount of HiPerfect Transfection Reagent and siRNA required for optimal performance may vary, depending on the cell line and gene target. When transfecting fluorescently labeled siRNA (e.g., Alexa Fluor 488 siRNA) for monitoring transfection efficiency under a fluorescent microscope, it may be necessary to increase the amount of siRNA up to 25 nM (without increasing the volume of HiPerFect Reagent), depending on the optical requirements of the microscopic equipment that will be used for detection.