How can air bubbles in PhosphoProtein Purification Columns be avoided?

Air bubbles forming in the columns of the PhosphoProtein Purification Kit can reduce the resin surface area available for protein binding, and thus reduce the binding capacity of the column. To avoid this, it is advisable to degas the PhosphoProtein Lysis Buffer prior to equilibrating the column. Another possibility is to mix the resin with the column storage buffer by inverting the column several times, and let the resin resettle. Always detach the top cap first before opening the bottom closure.