QIAsymphony SP_AS – Flexible Input, Quality Output

Sample types Input sample amount Elution volume (µl) Nucleic acid yield (µg) DNA from blood samples Whole blood 200 µl 200 4–12 Whole blood 400 µl 400 8–24 Whole blood 1000 µl 500 15–45 Buffy coat 200 µl 200 12–40 Buffy coat 400 µl 400 24–72 cfDNA from cell-free body fluids Plasma 200–4000 µl 60 ~5 ng/ml plasma DNA from tissues and cells Cultured cells 1x107 200 40–80 Spleen 25 mg 200 40–80 Muscle 50 mg 100 5–15 Lung 25 mg 200 10–25 Kidney 25 mg 200 15–30 Mouse tail 1 mg 200 20–40 Escherichia coli 4x109 200 50–70 FFPE tissues 1–8 sections 50–200 sample dependent DNA from forensic samples HID sample lysates 200–1000 µl 30–400 sample dependent DNA from food testing samples Bacterial enrichment culture 500 µl 100–400 sample dependent gDNA from food samples 500 µl 100–400 sample dependent RNA from tissues and cells Cultured cells 1x106 100, 200 10–25 Kidney 10 mg 100, 200 20–30 Liver 10 mg 100, 200 40–60 Spleen 10 mg 100, 200 30–40 Lung 10 mg 100, 200 10–20 Skin 10 mg 100, 200 4–8 Brain 10 mg 100, 200 3–10 FFPE tissue 1–5 sections 50, 100, 200 sample dependent Table 1. Specifications for nucleic acid purification from a range of sample types. QIAsymphony SP/AS 08/2016 9 DNA from blood samples Whole blood 200 µl 2004–12 Whole blood 400 µl 4008–24 Whole blood 1000 µl 50015–45 Buffy coat 200 µl 20012–40 Buffy coat 400 µl 40024–72 20040–80 Spleen 25 mg 20040–80 Muscle 50 mg 1005–15 Lung 25 mg 20010–25 Kidney 25 mg 20015–30 Mouse tail 1 mg 20020–40 20050–70 100, 20010–25 Kidney 10 mg 100, 20020–30 Liver 10 mg 100, 20040–60 Spleen 10 mg 100, 20030–40 Lung 10 mg 100, 20010–20 Skin 10 mg 100, 2004–8 Brain 10 mg 100, 2003–10

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