QIAsymphony SP_AS – Flexible Input, Quality Output - Seamlessly integrated PCR assay setup

5 10 0 0.1 0.2 0.3 0.4 0.5 15 20 Cycle Threshold Threshold Norm. Fluoro. 0 0.1 0.2 0.3 0.4 0.5 Norm. Fluoro. 25 30 35 40 5 10 15 20 Cycle 25 30 35 40 Manual QIAsymphony AS 5 10 0 0.1 0.2 0.3 0.4 0.5 15 20 Cycle Threshold Threshold Norm. Fluoro. 0 0.1 0.2 0.3 0.4 0.5 Norm. Fluoro. 25 30 35 40 5 10 15 20 Cycle 25 30 35 40 Manual QIAsymphony AS Seamlessly integrated PCR assay setup QIAsymphony AS extends the capabilities of the QIAsymphony SP by integrating automated PCR assay setup, enabling you to optimize your PCR workflow and maximize your efficiency (Figure 3). • Interfaced and connected with QIAsymphony SP • Dedicated software and validated protocols for ease of use (Figure 4) • Full temperature control and active cooling of templates, reagents and reaction mixes • Liquid level detection and reliable 4-channel system for PCR setup • Setup of multiple different PCRs per run • Integrated or independent operation • Bi-directional data exchange with LIMS Figure 3. Highly reproducible assay setup. A serial dilution of leukocyte cDNA (100 ng to 0.01 ng) was performed A. manually and B. using the QIAsymphony AS. GAPDH was amplified from these dilutions, using the Rotor-Gene® Q and the QuantiFast® Probe PCR Kit. Reactions were set up in replicates of 4 in strip tubes. A B 6 QIAsymphony SP/AS 08/2016 510 1520 25303540 5101520 25303540 510 1520 25303540 5101520 25303540

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