QIAsymphony SP_AS – Flexible Input, Quality Output - High quality nucleic acids, ready for analysis

High quality nucleic acids, ready for analysis QIAsymphony SP uses magnetic bead technology to streamline and automate nucleic acid purification from a wide range of starting materials (Table 1). Optimized protocols provide high yields of pure DNA (Figure 5A) and RNA (Figure 6), even from FFPE tissues (Figure 5B, C). Nucleic acids purified with QIAsymphony SP are free from inhibitors and ready for use in downstream analyses such as PCR, qPCR and RT-PCR. Figure 5. High yield and purity. A. DNA was purified from human blood in replicates of 4 using the QIAsymphony DSP DNA Kit. The purified DNA was of high yield and purity, with A260 /A280 >1.8, regardless of the starting volume (not shown). B. DNA was purified from human spleen FFPE sections using the QIAsymphony DSP DNA Kit. The yield increases linearly with the starting sample amounts. C. DNA was purified from human FFPE tissues manually using the QIAamp® DSP DNA FFPE Tissue Kit or automated using QIAsymphony and the QIAsymphony DSP DNA Kit. Automated purification is as reliable as the manual method. 10 QIAsymphony SP/AS 08/2016 50 40 1 2 Donor 3 0 DNA Yield (µg) 20 30 10 200 µl protocol 1000 µl protocol A 12 10 6 4 Number of 10 µl sections 4.5 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 0 DNA Yield (µg) 2 8 Human spleen Linear (Human spleen) R2 = 0.9963 B 8 6 Kidney Colon Lung 0 DNA Yield (µg) 2 4 QIAsymphony QIAamp C 25 20 QIAsymphony Run A QIAsymphony Run B RNeasy Mini 0 RNA Yield (µg) 10 15 5 2 x 106 cells 1 x 106 cells 0.5 x 106 cells 50 40 20 10 Time (s) 65 20 25 30 35 40 28S 18S 45 50 55 60 0 Fluorescence 30 Figure 6. High yields of high-quality RNA. A. RNA was purified from Jurkat cells manually using the RNeasy® Mini Kit or automated using QIAsymphony SP and the QIAsymphony RNA Kit. The automated procedure performed as well as the trusted manual procedure. B. RNA purified from cultured Jurkat cells using QIAsymphony SP had RIN values between 9.9 and 10 and 28S/18S rRNA ratios of 2.0–2.1, indicating intact and high-quality RNA. B A 12 0.51.01.52.02.53.03.54.0 2025303540 45505560

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