Product FAQ - QIAGEN



Product FAQ Bookmark Share more.. You are not authorized to download the resource Sort options Sort alphabetically Sort by relevance Sorted alphabetically Will the 2x QIAGEN Multiplex PCR Master Mix freeze at -20°C? FAQ ID - 525 View Will the "classic" RNeasy Mini Kit be discontinued after the launch of the Allprep DNA/RNA Mini Kit? FAQ ID -1054 View Will TA cloning work with REPLI-g WGA product? FAQ ID -672 View Will site-specific biotin labeling with the EasyXpress Site-Specific Biotin kit alter the structure and function of my protein? FAQ ID -877 View Will Rotor-Gene Kits also work on the Rotor-Gene 6000 and 3000 cyclers? FAQ ID -2121 View Will REPLI-g work at high temperatures? FAQ ID -656 View Will QuantiTect Primer Assays work with Rotor-Gene SYBR Green Kits using an annealing step at 60ºC? FAQ ID -2124 View Will QuantiTect Primer Assays work at an annealing temperature of 60ºC with QuantiFast SYBR Green PCR and RT-PCR Kits? FAQ ID -1553 View Will QIAseq FastSelect work in other species? FAQ ID -147911 View Will qBiomarker Copy Number Assays work with heterogeneous samples like mixtures of tumor and normal tissue? FAQ ID — 3419 View Will proteins eluted in the Qproteome Plasma Membrane Protein protocol be modified, e.g. with the ligand? FAQ ID -1195 View Will pipetting error affect the miRNA qPCR Assay results? FAQ ID -2728 View Will pipetting error affect the EpiTect Methyl qPCR Array results? FAQ ID -2741 View Will pancreas tissue swim on top of RNAprotect Tissue Reagent? FAQ ID -536 View Will mitochondrial DNA also be isolated using the DNA Tissue protocols on the QIAsymphony SP? FAQ ID -1921 View Will mitochondrial DNA also be isolated using the DNA tissue protocols and QIAsymphony DSP DNA Mini Kit on the QIAsymphony SP? FAQ ID -2991 View Will integral, peripheral as well as lipid-anchored plasma membrane proteins be isolated with the Qproteome Plasma Membrane Protein Kit? FAQ ID -1199 View Will dUTP in a PCR reaction affect pyrosequencing? FAQ ID -2843 View Will DNA from chromosomes collected in TE with a drop of mineral oil work with REPLI-g amplification? FAQ ID -664 View Will biotin detection assays be efficient when doing in vitro translation from E. coli lysates with the EasyXpress Site-Specific Biotin Kit? FAQ ID -878 View Will acetone precipitation recommended in the Qproteome Protocols denature Protein? FAQ ID -807 View Why would clumps occur following the addition of Buffer P2 when using LyseBlue Reagent in a plasmid preparation? FAQ ID -862 View Why should I work with diluted or undiluted screens? FAQ ID -2419 View Why should I use the RNeasy Microarray Tissue Mini Kit when purifying RNA for microarray analysis? FAQ ID -2188 View Why should I use the EpiTect Plus FFPE Bisulfite Kit instead of isolating FFPE DNA first and then doing the bisulfite conversion? FAQ ID -2412 View Why should I use RT² SYBR Green Mastermix with RT² qPCR Primer Assays? FAQ ID -2706 View Why should DNA or cDNA targets be less than 250 bp long for real-time PCR? FAQ ID-751 View Why might my gene of interest drop out during WGA if it is not near a telomere or centromere? FAQ ID -703 View Why might Affymetrix GeneChip Mapping assays interfere with the REPLI-g FFPE kit? FAQ ID -1755 View Why is VeraSeq 2.0 DNA Polymerase sometimes cloudy upon removing from -20°C storage? FAQ ID - 3913 View Why is there RNA in the DNA eluate when using the AllPrep DNA Mini Spin Column? FAQ ID -1044 View Why is there no vacuum-only protocol for the RNeasy Plus 96 Kit? FAQ ID -1993 View Why is there no QuantiTect Primer Assay for my gene of interest? FAQ ID -1140 View Why is there no male DNA obtained from sexual assault samples? FAQ ID - 143767 View Why is there no detection of free reporter fluorescence in the LiquiChip System? FAQ ID -732 View Why is there more than one QIAgene for my gene of interest? FAQ ID -2044 View Why is there DNA in the no-template control reaction when using the standard REPLI-g procedure, but not when using the UltraFast procedure? FAQ ID -1327 View Why is there DNA in the no-template (negative) control when using the QuantiTect Whole Transcriptome Kit? FAQ ID -1620 View Why is there an asterisk for my miScript Primer Assays? FAQ ID -2176 View Why is there a need to overlay the resuspended DNA pellet with PRS again (step 17 of QSP)? FAQ ID - 3951 View Why is the Type-it Mutation Detect PCR Kit recommended for preamplification of SNPs? FAQ ID -2067 View Why is the storage time for QuantiFast PCR Kits shorter than that for QuantiTect PCR Kits? FAQ ID -1446 View Why is the RT step with the QuantiFast RT Kits much shorter compared to QuantiTect RT Kits? FAQ ID -1451 View Why is the reaction volume for QuantiFast PCR Kits lower than that for QuantiTect PCR Kits? FAQ ID -1447 View Why is the QuantiFast denaturation step different for PCR and RT-PCR runs in the two-step protocol for the ABI 7500 and other cyclers? FAQ ID -1442 View Why is the qRT-PCR reproducibility so critical when detecting gene expression knock down in an RNAi experiment? FAQ ID -2774 View Why is the QIAcube Connect App reacting so slowly? FAQ ID - 141519 View Why is the QIAamp Viral RNA Mini Kit (50) currently unavailable? FAQ ID -147395 View Why is the pQE DNA provided in QIAexpress Kits blue in color? FAQ ID -487 View Why is the Phoenix Hot Start Taq sometimes cloudy upon removing from -20°C storage? FAQ ID - 3906 View Why is the magnesium (Mg2+) solution supplied in a separate tube in the artus® Parvo B19 PCR Kits? FAQ ID -1533 View Why is the initial fluorescence signal too high when using artus PCR assays on the Rotor-Gene cycler? FAQ ID -1497 View Why is the initial activation step different for Rotor-Gene Probe, SYBR Green and Multiplex Kits? FAQ ID -2118 View Why is the IC not detectable although the analytical PCR is positive? FAQ ID -1505 View Why is the IC not detectable although the analytical PCR is negative? FAQ ID -1506 View Why is the fluorescence signal of the Internal Control on my Rotor-Gene Q very low? FAQ ID -2574 View Why is the final heat denaturation step so important with the QIAsymphony PAXgene Blood RNA protocol? FAQ ID -2987 View Why is the copy number call lower than expected? FAQ ID — 3413 View Why is the activation time for HotStarTaq Plus Polymerase in the QuantiFast SYBR Green Kits different from that for QuantiFast Probe Kits? FAQ ID -1449 View Why is special Type-it Fast SNP Probe PCR chemistry required for TaqMan® SNP Genotyping? FAQ ID -2057 View Why is my plasmid DNA yield low? FAQ ID -768 View Why is my no template control (NTC) real-time Ct value < 35 cycles in my qPCR Assay? FAQ ID -2686 View Why is my EpiTect Methyl qPCR Assay "failed" as indicated in the QC page of the data analysis Excel file? FAQ ID -2734 View Why is my 260/280 ratio low after using the DNeasy mericon Food Kit? FAQ ID - 3349 View Why is maximum amplicon size for the Type-it Microsatellite PCR Kit limited to 500 bp? FAQ ID -2060 View Why is it recommended to add 1 mM IPTG for optimal protein yields using the EasyXpress Protein Synthesis Kits? FAQ ID -860 View Why is it not recommended to stabilize cells with RNAprotect Tissue Reagent? FAQ ID -941 View Why is EvaGreen instead of SYBR Green used as fluorescent dye in the Type-it HRM PCR Kit? FAQ ID -2196 View Why is DTT used only for semen samples? FAQ ID - 143761 View Why is CoralLoad included in the Type-it Mutation Detect, but not in the Type-it Microsatellite PCR Kit? FAQ ID -2068 View Show more results Back to top Contact QIAGEN Global contacts Technical Service Customer Care