Why is there no detection of free reporter fluorescence in the LiquiChip System?

The detection of the green reporter signal is triggered by the detection of the red classification fluorescence. Therefore, a specific sample value is only recorded if a red bead classification signal and a green reporter signal are measured simultaneously.

In addition, the assay mixture is covered by the LiquiChip System Fluid for the measurement in the capillary, so that only a small amount of assay mixture is around the beads during measurements. Further information can be found online on the product page for the LiquiChip System.