Product FAQ - QIAGEN



Product FAQ Bookmark Share more.. You are not authorized to download the resource Sort options Sort alphabetically Sort by relevance Sorted alphabetically Why do you recommend using Triton X for the purification of 6xHis-tagged protein? -100 View Why do the Internal Control templates for extraction (Internal Control DNA or RNA [High conc.]) have a 10x higher concentration than the IC templates provided with the QuantiFast Pathogen PCR +IC Kit and the QuantiFast Pathogen RT-PCR +IC Kit? FAQ ID -2603 View Why do the amplification curves generated with ABI PRISM® 7000 SDS instrument look rather zigzag-shaped when compared to results generated with the ABI PRISM® 7700 SDS instrument? FAQ ID -1522 View Why do replicates in real-time PCR have different plateau heights? FAQ ID -539 View Why do QuantiFast SYBR Green PCR Kits require such a high primer concentration? FAQ ID -1443 View Why do qBiomarker Copy Number PCR Arrays have assays in quadruplicate? FAQ ID — 3414 View Why do pQE-T7 vectors of the QIAgenes E. coli system have an additional UAG amber stop codon? FAQ ID -2043 View Why do my qPCR amplification curves or plots decrease in fluorescence intensity after the saturation phase? FAQ ID -2689 View Why do melting temperatures differ between PCR fragments amplified with QIAGEN's QuantiTect SYBR Green PCR Kits and Roche Kits? FAQ ID -1084 View Why do I sometimes get light blue colonies when using the QIAGEN PCR Cloning Kit? FAQ ID -603 View Why do I see multiple high-intensity peaks in my qPCR dissociation curve at temperatures less than 70 ºC? FAQ ID -2690 View Why do I see low, poor, or sub-standard amplification efficiency in my qRT-PCR assay? FAQ ID -2695 View Why do I observe a CT shift for the Internal Control in some samples in comparison to the CT value for the Internal Control of the dilution series of the Male Control DNA M1? FAQ ID - 3745 View Why do I need to identify my real-time instrument model when placing my order for RT² qPCR Primer Assays? FAQ ID -2713 View Why do I need normalization using Rotor-Gene ScreenClust HRM Software? FAQ ID -2198 View Why do I have wavy DNA bands on my agarose gel? FAQ ID -2260 View Why do I have to use an ACS (Assay Control Set)? FAQ ID -2933 View Why do I have to add beta-mercaptoethanol (beta-ME) to lysis Buffer RLT of the RNeasy Kits? FAQ ID -101 View Why do I get smeared PCR products? FAQ ID -87 View Why do I get genomic DNA contamination in my plasmid prep? FAQ ID -353 View Why do I get different results for the loci SE33 if I analyze the same sample using the Investigator Nonaplex ESS Kit or Investigator Triplex AFS QS Kit? FAQ ID -2401 View Why do I get amplification in a negative control DNA tube using the REPLI-g Kit for WGA? FAQ ID -675 View Why do casework-sample protocols for the QIAsymphony DNA Investigator Kit start with different lysate volumes (200 µl, 500 µl, or 1000 µl)? FAQ ID -2035 View Why did you choose ORF1a+ORF1b and ORF3a+ORF7a? FAQ ID - 3893 View Why did you choose MAPK1 as a positive control in the QIAGEN RNAi Human/Mouse Control Kit? FAQ ID -604 View Why did we not choose commonly used genes such as the S gene, E gene, or RdRP? FAQ ID - 3892 View Why did the real-time PCR yield Ct values < 12? FAQ ID -2727 View Why can’t I see my QIAcube Connect instrument in the QIAcube Connect App? FAQ ID - 1414518 View Why can't I find the DNA methylation qPCR Assay for my gene of interest? FAQ ID -2743 View Why can I not find the QuantiTect Primer Assay in GeneGlobe that I had previously ordered from QIAGEN? FAQ ID -1138 View Why can I not find the Q-Base device in the device list during the first installation? FAQ ID - 141516 View Why are two reagents used in the PAXgene Tissue System? FAQ ID -3022 View Why are two reagents used in the PAXgene Tissue System? FAQ ID - 3603 View Why are there various different QuantiTect Primer Assays for my gene (previous versions, transcript variants)? FAQ ID -1133 View Why are there various different QuantiFast Probe Assays for my gene (previous versions, transcript variants)? FAQ ID -2366 View Why are the RT² qPCR Primer Assays not designed to cross exon-intron junctions or boundaries? FAQ ID -2710 View Why are the QuantiTect and QuantiFast Multiplex PCR Kits limited to triplex real-time PCR on some cyclers? FAQ ID -715 View Why are the QuantiFast Probe Assays located on a single exon, thereby also detecting genomic DNA? FAQ ID -2365 View Why are the fluorescence intensities of the HSV detection markedly lower than those of the HSV-1 detection using the artus® HSV 1/2 LC PCR Kit? -2 View Why are the denaturation and annealing/extension times for QuantiFast Multiplex RT-PCR Kits much shorter than those for QuantiFast Multiplex PCR Kits? FAQ ID -2147 View Why are the centrifugation speeds for the QIAamp DNA Mini kit at 6000 x g? Can I spin at full speed? FAQ ID -474 View Why are some of my samples outside of the cluster using Rotor-Gene ScreenClust HRM Software? FAQ ID -2204 View Why are some of my RNAprotect-stabilized samples frozen at 0ºC while others are not? FAQ ID -758 View Why are samples centrifuged at 4°C after the addition of chloroform when using RNeasy Lipid Tissue Kits? FAQ ID -533 View Why are samples centrifuged at 4°C after addition of chloroform to the QIAzol lysates in following kits: RNeasy Lipid Tissue Mini/Midi kits, RNeasy Microarray Tissue Mini kit, RNeasy Plus Universal Mini/Midi kit, RNeasy 96 Universal Tissue kit, miRNeasy Mini kit, miRNeasy Micro kit, miRNeasy 96 kit and miRNeasy Serum/Plasma kit? FAQ ID -2345 View Why are not all applications/kits/protocol files visible in the run setup (QIAcube Connect App)? FAQ ID - 1414520 View Why are my realtime PCR amplification plots hook-shaped? FAQ ID -587 View Why are my qPCR Ct values too low (< 12) in my qRT-PCR Assay? FAQ ID -2684 View Why are my qPCR Ct values too high (> 35 or not detectable) in my qRT-PCR assay? FAQ ID -2685 View Why are my DV200 values of my RNA sample so low? FAQ ID - 3957 View Why are most of my samples outside of the cluster in supervised mode using Rotor-Gene ScreenClust HRM Software? FAQ ID -2205 View Why and when is it necessary to use carrier DNA/RNA in the extraction procedure? FAQ ID -1489 View Who can help in case of issues with the third party instrument? 3799 View Who can help if it is not clear if issues are caused by the third party instrument or by the chemistry? 3802 View Which version of the EZ2 Connect can run the EZ2 RNA/miRNA Tissue/Cells Kit? FAQ ID - 3851 View Which version of the EZ2 Connect can run the EZ2 RNA FFPE Kit? FAQ ID - 3940 View Which version of the EZ2 Connect can run the EZ2 AllPrep DNA/RNA FFPE Kit? FAQ ID - 3947 View Which variants of the EasyXtal 15-well tool are available? FAQ ID -2221 View Which types of collection tubes should preferentially be used for blood samples? FAQ ID -1496 View Which types and amounts of sample material can be processed for RNA extraction on the QIAsymphony SP? FAQ ID -1937 View Which tubes are recommended for the QIAsymphony sample rack FAQ ID - 3653 View Which transfection reagent is recommended for transfection of the miScript Target Protectors? FAQ ID -2252 View Which thermal cyclers are validated for use with the Investigator Nonaplex ESS Kit? FAQ ID -2392 View Which thermal cyclers are validated for use with the Investigator IDplex Kit? FAQ ID -2403 View Which software updates are included in a Premium Cover Agreement for my QIAGEN Instrument? FAQ ID -1336 View Which settings for the filter set Any5Dye must be adjusted before running the spectral calibration for Investigator IDplex Plus Kit? FAQ ID -3018 View Which settings for the filter set Any5Dye must be adjusted before running the spectral calibration for Investigator ESSplex SE Plus Kit? FAQ ID -3031 View Which settings for the filter set Any5Dye have to be adjusted before running the spectral calibration on the Investigator ESSplex Plus? FAQ ID -2649 View Which SARS-CoV-2 targets can the SARS-CoV-2 Neo Kit detect? FAQ ID - 3881 View Which sample volumes can be used for the QIAsymphony DSP Circulating DNA Kit? FAQ ID - 3700 View Which sample types can be processed using the QIAsymphony Virus Blood 200 protocol? FAQ ID -2154 View Which sample types can be processed using the QIAsymphony PAXgene Blood RNA Protocol? FAQ ID -2981 View Which sample types can be processed using the PAXgene Blood miRNA protocols? FAQ ID - 3478 View Which sample materials can be tested using the artus Infl./H1 LC/RG RT-PCR Kit? FAQ ID -2209 View Which sample materials are validated within the CE mark for the artus HSV/2 RG PCR -1 View Which sample material is validated within the CE mark for artus VZV RG PCR Kit? FAQ ID -2231 View Which Rotor-Gene Q rotor should be used with the therascreen EGFR RGQ PCR kit? FAQ ID -2312 View Which Rotor-Gene Q rotor should be used with the EGFR RGQ PCR kit? FAQ ID -2315 View Which resin is used in the QIAexpress Ni-NTA Fast Start Columns? FAQ ID -836 View Which regions are covered by the GR DNAseq V2 Panels (#181900)? FAQ ID - 3688 View Which real-time PCR kits are recommended downstream of the QuantiTect Whole Transcriptome Kit? FAQ ID -1592 View Which real-time PCR files should customers forward when they want their data to be re-analyzed by artus® technical support? FAQ ID -1508 View Which real-time PCR cyclers can be used with the artus Infl./H1 LC/RG RT-PCR Kits? FAQ ID -2208 View Which QuantiTect Primer Assay should I choose for my gene of interest? FAQ ID -1135 View Which Qproteome or Protein Fractionation Kits from QIAGEN are compatible with tissue samples? FAQ ID -755 View Which qPCR instrument should I use with your RT² qPCR Primer Assays? FAQ ID -2714 View Which QIAsymphony Virus/Bacteria Kit size should be used for each protocol? FAQ ID -2038 View Which QIAGEN plasmid preparation kits will contain LyseBlue Reagent? FAQ ID -865 View Which QIAGEN kit do you recommend for purifying plasmid DNA suitable for transfection of sensitive cells? FAQ ID -1092 View Which QIAGEN DNA extraction kits are compatible with the PAXgene Saliva Collector? 3821 View Which QIAcube Connect standard protocol might be suitable to extract RNA from saliva or from a buccal cell pellet? FAQ ID - 141508 View Which PyroMark Gold Q96 Reagent should be used for which instrument and application? FAQ ID -2836 View Which purity grade is recommended for pyrosequencing primers? FAQ ID -2832 View Which purification kits are recommended for the therascreen Pyro kits? FAQ ID -2386 View Which purification kits are recommended for the Pyro kits? FAQ ID -2380 View Which protocol line should I use to process plasma samples? FAQ ID - 3654 View Which probe labels are available for the QuantiFast Probe Assays? FAQ ID -2361 View Which probe labels are available for the Microbial DNA qPCR Assays? FAQ ID — 3407 View Which primers can I use for sequencing pQE-expression vector constructs? FAQ ID -343 View Which positive control should I use? FAQ ID - 3896 View Which plate formats are offered for QuantiTect Primer Assay Plates? FAQ ID -2106 View Which plasma volumes can I process with the QIAsymphony PAXgene Blood ccfDNA protocols? FAQ ID - 3648 View Which pathogens can be detected using the artus Infl./H1 LC/RG RT-PCR Kit? FAQ ID -2206 View Which parts are exchanged during a Preventive Maintenance visit for my QIAGEN BioRobot? FAQ ID -1334 View Which operating system is compatible with PyroMark Q24 Advanced application software? FAQ ID -3164 View Which operating system is compatible with PyroMark IdentiFire Software? FAQ ID - 3340 View Which nucleotide analogs can be used with TopTaq DNA Polymerase? FAQ ID -1741 View Which network settings are supported by the QIAcube Connect and Q-Base? FAQ ID - 141517 View Which mode should I use in the Rotor-Gene ScreenClust HRM Software, supervised or unsupervised? FAQ ID -2202 View Which Microbial qPCR Mastermix should I use? FAQ ID — 3395 View Which materials can be used as input samples for the QIAsymphony DSP Circulating DNA Kit? FAQ ID - 3702 View Which kits can be used in combination with the PyroMark CpG Assays and PyroMark Custom Assays? FAQ ID -2822 View Which kits and protocols can be used to isolate nucleic acids from microdissected PAXgene Tissue fixed, paraffin-embedded (PFPE) and PAXgene Tissue fixed, cryo-embedded (PFCE) specimens? FAQ ID - 3617 View Which kit, the QIAprep 96 Plus Miniprep Kit or the QIAGEN Plasmid Plus 96 Miniprep Kit, do you recommend for transfection? FAQ ID -2320 View Which kit size should be used for each QIAsymphony DSP Virus/Pathogen protocol? FAQ ID -2930 View Which kit should I use for RNA isolation from Cartilage? FAQ ID -1026 View Which kit should be used to extract RNA from adipose tissue, brain, and other fatty animal tissues? FAQ ID -467 View Which kit can be used for simultaneous purification of DNA and RNA from biopsies? FAQ ID -1089 View Which kind of 96 and 384-well plates are available for FlexiPlate siRNAs? FAQ ID -1459 View Which is the best condition for eluting GST-tagged proteins from Glutathione Superflow? FAQ ID -2173 View Which human target is used for the quantification in the Investigator Quantiplex Kit? FAQ ID -2577 View Which homogenization method do you recommend for tissues stored in Allprotect Tissue Reagent? FAQ ID -1572 View Which heating block is recommended for the pyrosequencing annealing step? FAQ ID -2838 View Which fraction will contain soluble mitochondrial proteins using the Qproteome Cell Compartment Kit? FAQ ID -839 View Which fraction will contain endosomal, microsomal and lysosomal proteins using the Qproteome Cell Compartment Kit? FAQ ID -808 View Which formats are available for FlexiPlate siRNA? FAQ ID -1358 View Which fixation method is used in the PAXgene Tissue System? FAQ ID - 3600 View Which filter set should be used with the Investigator ESSplex Plus? FAQ ID -2648 View Which filter set should be used with Investigator IDplex Plus Kit? FAQ ID -3017 View Which filter set should be used with Investigator ESSplex SE Plus Kit? FAQ ID -3030 View Which filter set and settings should be used with the Investigator Nonaplex ESS Kit? FAQ ID -2396 View Which extraction kits are recommended? FAQ ID - 3874 View Which expression systems can be used with the QIAgenes Insect/Mammalia expression constructs? FAQ ID -2049 View Which EpiTect Bisulfite Plus kit do you recommend for blood samples, cells, and tissues? FAQ ID -2411 View Which end of the PCR primer for pyrosequencing should be biotinylated? FAQ ID -2839 View Which elution volumes can be used for the QIAsymphony DSP Virus/Pathogen protocols? FAQ ID -2938 View Which elution volumes can be selected using the QIAsymphony Virus Blood 200 protocol? FAQ ID -2160 View Which elution volumes are available with the QIAsymphony PAXgene Blood ccfDNA Kit? FAQ ID - 3655 View Which elution volume is used for the QIAsymphony DSP Circulating DNA Kit? FAQ ID - 3703 View Which downstream applications have been tested with SARS-CoV-2-derived RNA purified from saliva collected into PAXgene Saliva Collector? 3828 View Which downstream applications have been tested with DNA purified from samples collected in PAXgene Blood DNA Tubes IVD? FAQ ID - 3512 View Which downstream applications have been tested with DNA purified from samples collected in PAXgene Blood DNA Tubes (IVD)? FAQ ID - 3384 View Which downstream applications have been tested with DNA purified from saliva collected into PAXgene Saliva Collector? 3827 View Which downstream applications have been tested with ccfDNA purified from whole blood collected and processed with the PAXgene Blood ccfDNA System? FAQ ID - 3658 View Which DNA/RNA extraction kits were tested in combination with the QuantiFast Pathogen PCR +IC Kit and the QuantiFast Pathogen RT-PCR +IC Kit? FAQ ID -2606 View Which DNA polymerases are included in artus® kits? FAQ ID -1386 View Which disinfectants and cleaning agents do you recommend for the BioRobot EZ1 DSP? FAQ ID -1561 View Which deparaffinization method can I use with the AllPrep DNA/RNA FFPE Kit? FAQ ID -2352 View Which density gel type is contained in the yellow or green MaXtract Tubes? FAQ ID -1315 View Which database versions are used for CRISPR-Q Custom PCR Assay and CRISPR-Q Sanger Primers design? 3788 View Which crystallization solutions are compatible with lipidic cubic phase crystallization? FAQ ID -2418 View Which controls can I choose for FlexiPlate siRNA? FAQ ID -1368 View Which conditions are recommended for the storage of tissues in PAXgene Tissue STABILIZER? FAQ ID - 3605 View Which competent E. coli cells should I use to amplify the SureSilencing shRNA Plasmids? FAQ ID -2890 View Which chloroform should I use for the RNeasy Lipid Tissue Kit? FAQ ID -516 View Which chemical products do you use to prepare screening solutions for protein crystallization? FAQ ID -1122 View Which cell lines have been tested with FastLane Cell RT-PCR Kits? FAQ ID -1485 View Which cell collection methods are compatible with REPLI-g Single Cell RNA Library Kit? FAQ ID - 3555 View Which cell collection methods are compatible with REPLI-g Single Cell DNA Library Kit? FAQ ID - 3543 View Which capillary sequencers can be used for detection of amplicons from the Type-it Microsatellite PCR Kits? FAQ ID -2063 View Which BioRobot EZ1 instruments can be upgraded to become CE-IVDcompliant? FAQ ID -1560 View Which barcodes on sample tubes are accepted by the QIAsymphony SP? FAQ ID - 3598 View Which Anti-His Antibody is the most sensitive for my protein of interest? FAQ ID -172 View Which analyses can be performed with the PyroMark Q96 MD software? FAQ ID -2866 View Which analyses can be performed with the PyroMark Q96 ID software version 1.0? FAQ ID -2845 View Where to look for ordering Custom siRNAs online? FAQ ID -3106 View Where should I add a dye-label modification on the siRNA? FAQ ID -648 View Where can I order the Streptavidin Sepharose beads for pyrosequencing? FAQ ID -2850 View Where can I find the template files for the Investigator IDplex Plus Kit? FAQ ID -3019 View Where can I find the template files for the Investigator ESSplex SE Plus Kit? 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