Product FAQ

 

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Sorted alphabetically
Why is the Type-it Mutation Detect PCR Kit recommended for preamplification of SNPs?
FAQ ID -2067
Why is the storage time for QuantiFast PCR Kits shorter than that for QuantiTect PCR Kits?
FAQ ID -1446
Why is the RT step with the QuantiFast RT Kits much shorter compared to QuantiTect RT Kits?
FAQ ID -1451
Why is the reaction volume for QuantiFast PCR Kits lower than that for QuantiTect PCR Kits?
FAQ ID -1447
Why is the QuantiFast denaturation step different for PCR and RT-PCR runs in the two-step protocol for the ABI 7500 and other cyclers?
FAQ ID -1442
Why is the qRT-PCR reproducibility so critical when detecting gene expression knock down in an RNAi experiment?
FAQ ID -2774
Why is the QIAcube Connect App reacting so slowly?
FAQ ID - 141519
Why is the QIAamp Viral RNA Mini Kit (50) currently unavailable?
FAQ ID -147395
Why is the pQE DNA provided in QIAexpress Kits blue in color?
FAQ ID -487
Why is the Phoenix Hot Start Taq sometimes cloudy upon removing from -20°C storage?
FAQ ID - 3906
Why is the magnesium (Mg2+) solution supplied in a separate tube in the artus® Parvo B19 PCR Kits?
FAQ ID -1533
Why is the initial fluorescence signal too high when using artus PCR assays on the Rotor-Gene cycler?
FAQ ID -1497
Why is the initial activation step different for Rotor-Gene Probe, SYBR Green and Multiplex Kits?
FAQ ID -2118
Why is the IC not detectable although the analytical PCR is positive?
FAQ ID -1505
Why is the IC not detectable although the analytical PCR is negative?
FAQ ID -1506
Why is the fluorescence signal of the Internal Control on my Rotor-Gene Q very low?
FAQ ID -2574
Why is the final heat denaturation step so important with the QIAsymphony PAXgene Blood RNA protocol?
FAQ ID -2987
Why is the copy number call lower than expected?
FAQ ID — 3413
Why is the activation time for HotStarTaq Plus Polymerase in the QuantiFast SYBR Green Kits different from that for QuantiFast Probe Kits?
FAQ ID -1449
Why is special Type-it Fast SNP Probe PCR chemistry required for TaqMan® SNP Genotyping?
FAQ ID -2057
Why is my plasmid DNA yield low?
FAQ ID -768
Why is my no template control (NTC) real-time Ct value < 35 cycles in my qPCR Assay?
FAQ ID -2686
Why is my EpiTect Methyl qPCR Assay "failed" as indicated in the QC page of the data analysis Excel file?
FAQ ID -2734
Why is my 260/280 ratio low after using the DNeasy mericon Food Kit?
FAQ ID - 3349
Why is maximum amplicon size for the Type-it Microsatellite PCR Kit limited to 500 bp?
FAQ ID -2060
Why is it recommended to add 1 mM IPTG for optimal protein yields using the EasyXpress Protein Synthesis Kits?
FAQ ID -860
Why is it not recommended to stabilize cells with RNAprotect Tissue Reagent?
FAQ ID -941
Why is EvaGreen instead of SYBR Green used as fluorescent dye in the Type-it HRM PCR Kit?
FAQ ID -2196
Why is DTT used only for semen samples?
FAQ ID - 143761
Why is CoralLoad included in the Type-it Mutation Detect, but not in the Type-it Microsatellite PCR Kit?
FAQ ID -2068
Why is carrier RNA used during the isolation of gDNA from microdissected samples with the QIAamp DNA Micro Kit?
FAQ ID -473
Why is an ice-incubation step included during reaction set-up when following the QuantiTect RT-PCR but not the QuantiTect PCR protocol.
FAQ ID -283
Why is a blood collection set required to collect blood into the PAXgene Blood ccfDNA Tube?
FAQ ID - 3628
Why is a blood collection set required to be used with the PAXgene Blood RNA tube?
FAQ ID - 3459
Why is a 2-step (and not a 3-step) cycling protocol recommended for Rotor-Gene SYBR Green Kits?
FAQ ID -2122
Why is a 2-step (and not a 3-step) cycling protocol recommended for QuantiFast SYBR Green PCR Kits?
FAQ ID -1450
Why is 18S ribosomal RNA (rRNA) used as a housekeeping gene to normalize sample-to-sample, systematic variation in qPCR assays?
FAQ ID -2675
Why have the hazard symbols accompanying products changed?
FAQ ID - 3430
Why had my RT² SYBR Green Mastermix been working well in the past, but now does not seem to be?
FAQ ID -2717
Why does the upper aqueous phase look pinkish when purifying RNA from fatty tissue?
FAQ ID -3118
Why does the template for EasyXpress Disulfide Insect Kits need to have a signal sequence (such as the mellitin signal sequence as provided by the EasyXpress Linear Template Fab Kit primers)?
FAQ ID -2967
Why does the SeqTarget Prep Protocol require a 2-step procedure?
FAQ ID -2238
Why does the QuantiTect Primer Assay for my gene of interest have only one version number?
FAQ ID -1134
Why does the QIAcube sometimes fail to pick up the tips or pick up wrong tips?
FAQ ID -3131
Why does the QIAamp DNA Mini Tissue Protocol require both ATL and AL buffer, while the Blood Protocol only uses AL?
FAQ ID -633
Why does the miScript Target Protector not induce the degradation process?
FAQ ID -2263
Why does the fluorescence signal drop sharply at the beginning of an artus® PCR run?
FAQ ID -1498
Why does the DNeasy mericon Food Kit use a QIAquick column and Buffer PB rather than a DNeasy column and Buffer AL, which might be expected since the kit isolates genomic DNA?
FAQ ID - 3347
Why does the DNeasy mericon Food extraction kit use a QIAquick column and not DNeasy column?
FAQ ID -3148
Why does the copy function not apply relative to migration time and normalized area values after loading the DNA size marker table and adding the alignment marker peaks on the QIAxcel System?
FAQ ID -1832
Why does the connection test fail?
FAQ ID - 141522
Why does the Allprep DNA/RNA 96 Kit use Buffer RLT, whereas the AllPrep DNA/RNA Mini Kit uses Buffer RLT Plus?
FAQ ID -1999
Why does the A peak reduction factor have to de set to 0.86 for the codon 61 assay in the therascreen NRAS Pyro Kit?
FAQ ID -2390
Why does TA/UA cloning work with your proofreading HotStar HiFidelity DNA Polymerase?
FAQ ID -1053
Why does my realtime PCR assay quality decrease over time?
FAQ ID -589
Why does my purified DNA/RNA sometimes have a 260/280 or 260/230 ratio of more than 2? Does that mean the purity is poor?
FAQ ID -3132
Why does my PCR product show up later when comparing the QuantiTect SYBR Green PCR Kits with Roche kits using the same annealing temperature?
FAQ ID -1083
Why does my isolated RNA have a low OD 260/280 ratio?
FAQ ID -97
Why does my DNA sample float out of the slot when loading it onto an agarose gel?
FAQ ID -205
Why does AllPrep DNA/RNA/Protein Mini kit use buffer RLT, but Allprep DNA/RNA Mini and Allprep DNA/RNA Micro kits use buffer RLT Plus? Are these buffers interchangeable?
FAQ ID - 3391
Why do you recommend using Triton X for the purification of 6xHis-tagged protein?
-100
Why do the Internal Control templates for extraction (Internal Control DNA or RNA [High conc.]) have a 10x higher concentration than the IC templates provided with the QuantiFast Pathogen PCR +IC Kit and the QuantiFast Pathogen RT-PCR +IC Kit?
FAQ ID -2603
Why do the amplification curves generated with ABI PRISM® 7000 SDS instrument look rather zigzag-shaped when compared to results generated with the ABI PRISM® 7700 SDS instrument?
FAQ ID -1522
Why do replicates in real-time PCR have different plateau heights?
FAQ ID -539
Why do QuantiFast SYBR Green PCR Kits require such a high primer concentration?
FAQ ID -1443
Why do qBiomarker Copy Number PCR Arrays have assays in quadruplicate?
FAQ ID — 3414
Why do pQE-T7 vectors of the QIAgenes E. coli system have an additional UAG amber stop codon?
FAQ ID -2043
Why do my qPCR amplification curves or plots decrease in fluorescence intensity after the saturation phase?
FAQ ID -2689
Why do melting temperatures differ between PCR fragments amplified with QIAGEN's QuantiTect SYBR Green PCR Kits and Roche Kits?
FAQ ID -1084
Why do I sometimes get light blue colonies when using the QIAGEN PCR Cloning Kit?
FAQ ID -603
Why do I see multiple high-intensity peaks in my qPCR dissociation curve at temperatures less than 70 ºC?
FAQ ID -2690
Why do I see low, poor, or sub-standard amplification efficiency in my qRT-PCR assay?
FAQ ID -2695
Why do I observe a CT shift for the Internal Control in some samples in comparison to the CT value for the Internal Control of the dilution series of the Male Control DNA M1?
FAQ ID - 3745
Why do I need to identify my real-time instrument model when placing my order for RT² qPCR Primer Assays?
FAQ ID -2713
Why do I need normalization using Rotor-Gene ScreenClust HRM Software?
FAQ ID -2198
Why do I have wavy DNA bands on my agarose gel?
FAQ ID -2260
Why do I have to use an ACS (Assay Control Set)?
FAQ ID -2933
Why do I have to add beta-mercaptoethanol (beta-ME) to lysis Buffer RLT of the RNeasy Kits?
FAQ ID -101
Why do I get smeared PCR products?
FAQ ID -87
Why do I get genomic DNA contamination in my plasmid prep?
FAQ ID -353
Why do I get different results for the loci SE33 if I analyze the same sample using the Investigator Nonaplex ESS Kit or Investigator Triplex AFS QS Kit?
FAQ ID -2401
Why do I get amplification in a negative control DNA tube using the REPLI-g Kit for WGA?
FAQ ID -675
Why do casework-sample protocols for the QIAsymphony DNA Investigator Kit start with different lysate volumes (200 µl, 500 µl, or 1000 µl)?
FAQ ID -2035
Why did you choose ORF1a+ORF1b and ORF3a+ORF7a?
FAQ ID - 3893
Why did you choose MAPK1 as a positive control in the QIAGEN RNAi Human/Mouse Control Kit?
FAQ ID -604
Why did we not choose commonly used genes such as the S gene, E gene, or RdRP?
FAQ ID - 3892
Why did the real-time PCR yield Ct values < 12?
FAQ ID -2727
Why can’t I see my QIAcube Connect instrument in the QIAcube Connect App?
FAQ ID - 1414518
Why can't I find the DNA methylation qPCR Assay for my gene of interest?
FAQ ID -2743
Why can I not find the QuantiTect Primer Assay in GeneGlobe that I had previously ordered from QIAGEN?
FAQ ID -1138
Why can I not find the Q-Base device in the device list during the first installation?
FAQ ID - 141516
Why are two reagents used in the PAXgene Tissue System?
FAQ ID -3022
Why are two reagents used in the PAXgene Tissue System?
FAQ ID - 3603
Why are there various different QuantiTect Primer Assays for my gene (previous versions, transcript variants)?
FAQ ID -1133
Why are there various different QuantiFast Probe Assays for my gene (previous versions, transcript variants)?
FAQ ID -2366
Why are the RT² qPCR Primer Assays not designed to cross exon-intron junctions or boundaries?
FAQ ID -2710
Why are the QuantiTect and QuantiFast Multiplex PCR Kits limited to triplex real-time PCR on some cyclers?
FAQ ID -715
Why are the QuantiFast Probe Assays located on a single exon, thereby also detecting genomic DNA?
FAQ ID -2365
Why are the fluorescence intensities of the HSV detection markedly lower than those of the HSV-1 detection using the artus® HSV 1/2 LC PCR Kit?
-2
Why are the denaturation and annealing/extension times for QuantiFast Multiplex RT-PCR Kits much shorter than those for QuantiFast Multiplex PCR Kits?
FAQ ID -2147
Why are the centrifugation speeds for the QIAamp DNA Mini kit at 6000 x g? Can I spin at full speed?
FAQ ID -474
Why are some of my samples outside of the cluster using Rotor-Gene ScreenClust HRM Software?
FAQ ID -2204
Why are some of my RNAprotect-stabilized samples frozen at 0ºC while others are not?
FAQ ID -758
Why are samples centrifuged at 4°C after the addition of chloroform when using RNeasy Lipid Tissue Kits?
FAQ ID -533
Why are samples centrifuged at 4°C after addition of chloroform to the QIAzol lysates in following kits: RNeasy Lipid Tissue Mini/Midi kits, RNeasy Microarray Tissue Mini kit, RNeasy Plus Universal Mini/Midi kit, RNeasy 96 Universal Tissue kit, miRNeasy Mini kit, miRNeasy Micro kit, miRNeasy 96 kit and miRNeasy Serum/Plasma kit?
FAQ ID -2345
Why are not all applications/kits/protocol files visible in the run setup (QIAcube Connect App)?
FAQ ID - 1414520
Why are my realtime PCR amplification plots hook-shaped?
FAQ ID -587
Why are my qPCR Ct values too low (< 12) in my qRT-PCR Assay?
FAQ ID -2684
Why are my qPCR Ct values too high (> 35 or not detectable) in my qRT-PCR assay?
FAQ ID -2685
Why are my DV200 values of my RNA sample so low?
FAQ ID - 3957
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