Product FAQ - QIAGEN



Product FAQ Bookmark Share more.. You are not authorized to download the resource Sort options Sort alphabetically Sort by relevance Sorted alphabetically Will TA cloning work with REPLI-g WGA product? FAQ ID -672 View Will site-specific biotin labeling with the EasyXpress Site-Specific Biotin kit alter the structure and function of my protein? FAQ ID -877 View Will Rotor-Gene Kits also work on the Rotor-Gene 6000 and 3000 cyclers? FAQ ID -2121 View Will REPLI-g work at high temperatures? FAQ ID -656 View Will QuantiTect Primer Assays work with Rotor-Gene SYBR Green Kits using an annealing step at 60ºC? FAQ ID -2124 View Will QuantiTect Primer Assays work at an annealing temperature of 60ºC with QuantiFast SYBR Green PCR and RT-PCR Kits? FAQ ID -1553 View Will QIAseq FastSelect work in other species? FAQ ID -147911 View Will qBiomarker Copy Number Assays work with heterogeneous samples like mixtures of tumor and normal tissue? FAQ ID — 3419 View Will proteins eluted in the Qproteome Plasma Membrane Protein protocol be modified, e.g. with the ligand? FAQ ID -1195 View Will pipetting error affect the miRNA qPCR Assay results? FAQ ID -2728 View Will pipetting error affect the EpiTect Methyl qPCR Array results? FAQ ID -2741 View Will pancreas tissue swim on top of RNAprotect Tissue Reagent? FAQ ID -536 View Will mitochondrial DNA also be isolated using the DNA Tissue protocols on the QIAsymphony SP? FAQ ID -1921 View Will mitochondrial DNA also be isolated using the DNA tissue protocols and QIAsymphony DSP DNA Mini Kit on the QIAsymphony SP? FAQ ID -2991 View Will integral, peripheral as well as lipid-anchored plasma membrane proteins be isolated with the Qproteome Plasma Membrane Protein Kit? FAQ ID -1199 View Will dUTP in a PCR reaction affect pyrosequencing? FAQ ID -2843 View Will DNA from chromosomes collected in TE with a drop of mineral oil work with REPLI-g amplification? FAQ ID -664 View Will biotin detection assays be efficient when doing in vitro translation from E. coli lysates with the EasyXpress Site-Specific Biotin Kit? FAQ ID -878 View Will acetone precipitation recommended in the Qproteome Protocols denature Protein? FAQ ID -807 View Why would clumps occur following the addition of Buffer P2 when using LyseBlue Reagent in a plasmid preparation? FAQ ID -862 View Why should I work with diluted or undiluted screens? FAQ ID -2419 View Why should I use the RNeasy Microarray Tissue Mini Kit when purifying RNA for microarray analysis? FAQ ID -2188 View Why should I use the EpiTect Plus FFPE Bisulfite Kit instead of isolating FFPE DNA first and then doing the bisulfite conversion? FAQ ID -2412 View Why should I use RT² SYBR Green Mastermix with RT² qPCR Primer Assays? FAQ ID -2706 View Why should DNA or cDNA targets be less than 250 bp long for real-time PCR? FAQ ID-751 View Why might my gene of interest drop out during WGA if it is not near a telomere or centromere? FAQ ID -703 View Why might Affymetrix GeneChip Mapping assays interfere with the REPLI-g FFPE kit? FAQ ID -1755 View Why is VeraSeq 2.0 DNA Polymerase sometimes cloudy upon removing from -20°C storage? FAQ ID - 3913 View Why is there RNA in the DNA eluate when using the AllPrep DNA Mini Spin Column? FAQ ID -1044 View Why is there no vacuum-only protocol for the RNeasy Plus 96 Kit? FAQ ID -1993 View Why is there no QuantiTect Primer Assay for my gene of interest? FAQ ID -1140 View Why is there no male DNA obtained from sexual assault samples? FAQ ID - 143767 View Why is there no detection of free reporter fluorescence in the LiquiChip System? FAQ ID -732 View Why is there more than one QIAgene for my gene of interest? FAQ ID -2044 View Why is there DNA in the no-template control reaction when using the standard REPLI-g procedure, but not when using the UltraFast procedure? FAQ ID -1327 View Why is there DNA in the no-template (negative) control when using the QuantiTect Whole Transcriptome Kit? FAQ ID -1620 View Why is there an asterisk for my miScript Primer Assays? FAQ ID -2176 View Why is there a need to overlay the resuspended DNA pellet with PRS again (step 17 of QSP)? FAQ ID - 3951 View Why is the Type-it Mutation Detect PCR Kit recommended for preamplification of SNPs? FAQ ID -2067 View Why is the storage time for QuantiFast PCR Kits shorter than that for QuantiTect PCR Kits? FAQ ID -1446 View Why is the RT step with the QuantiFast RT Kits much shorter compared to QuantiTect RT Kits? FAQ ID -1451 View Why is the reaction volume for QuantiFast PCR Kits lower than that for QuantiTect PCR Kits? FAQ ID -1447 View Why is the QuantiFast denaturation step different for PCR and RT-PCR runs in the two-step protocol for the ABI 7500 and other cyclers? FAQ ID -1442 View Why is the qRT-PCR reproducibility so critical when detecting gene expression knock down in an RNAi experiment? FAQ ID -2774 View Why is the QIAcube Connect App reacting so slowly? FAQ ID - 141519 View Why is the QIAamp Viral RNA Mini Kit (50) currently unavailable? FAQ ID -147395 View Why is the pQE DNA provided in QIAexpress Kits blue in color? FAQ ID -487 View Why is the Phoenix Hot Start Taq sometimes cloudy upon removing from -20°C storage? FAQ ID - 3906 View Why is the initial activation step different for Rotor-Gene Probe, SYBR Green and Multiplex Kits? FAQ ID -2118 View Why is the fluorescence signal of the Internal Control on my Rotor-Gene Q very low? FAQ ID -2574 View Why is the final heat denaturation step so important with the QIAsymphony PAXgene Blood RNA protocol? FAQ ID -2987 View Why is the copy number call lower than expected? FAQ ID — 3413 View Why is the activation time for HotStarTaq Plus Polymerase in the QuantiFast SYBR Green Kits different from that for QuantiFast Probe Kits? FAQ ID -1449 View Why is special Type-it Fast SNP Probe PCR chemistry required for TaqMan® SNP Genotyping? FAQ ID -2057 View Why is my plasmid DNA yield low? FAQ ID -768 View Why is my no template control (NTC) real-time Ct value < 35 cycles in my qPCR Assay? FAQ ID -2686 View Why is my EpiTect Methyl qPCR Assay "failed" as indicated in the QC page of the data analysis Excel file? FAQ ID -2734 View Why is my 260/280 ratio low after using the DNeasy mericon Food Kit? FAQ ID - 3349 View Why is maximum amplicon size for the Type-it Microsatellite PCR Kit limited to 500 bp? FAQ ID -2060 View Why is it recommended to add 1 mM IPTG for optimal protein yields using the EasyXpress Protein Synthesis Kits? FAQ ID -860 View Why is it not recommended to stabilize cells with RNAprotect Tissue Reagent? FAQ ID -941 View Why is EvaGreen instead of SYBR Green used as fluorescent dye in the Type-it HRM PCR Kit? FAQ ID -2196 View Why is DTT used only for semen samples? FAQ ID - 143761 View Why is CoralLoad included in the Type-it Mutation Detect, but not in the Type-it Microsatellite PCR Kit? FAQ ID -2068 View Why is carrier RNA used during the isolation of gDNA from microdissected samples with the QIAamp DNA Micro Kit? FAQ ID -473 View Why is an ice-incubation step included during reaction set-up when following the QuantiTect RT-PCR but not the QuantiTect PCR protocol. FAQ ID -283 View Why is a blood collection set required to collect blood into the PAXgene Blood ccfDNA Tube? FAQ ID - 3628 View Why is a blood collection set required to be used with the PAXgene Blood RNA tube? FAQ ID - 3459 View Why is a 2-step (and not a 3-step) cycling protocol recommended for Rotor-Gene SYBR Green Kits? FAQ ID -2122 View Why is a 2-step (and not a 3-step) cycling protocol recommended for QuantiFast SYBR Green PCR Kits? FAQ ID -1450 View Show more results Back to top Contact QIAGEN Global contacts Technical Service Customer Care