Why is my 260/280 ratio low after using the DNeasy mericon Food Kit?
With complex food samples, a classical evaluation of DNA purity and protein content by A260/A280 ratio is not a significant criterion. Due to inevitable traces of food matrix in the sample background, a spectroscopic evaluation of DNA or protein absorptions is highly unreliable, and we have found that there is not a correlation between 260/280 and 260/230 ratios and PCR performance. Even DNA with Low 260/280 and 260/230 can produce good PCR results.