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Why does the QIAamp DNA Mini Tissue Protocol require both ATL and AL buffer, while the Blood Protocol only uses AL?
FAQ ID -633
View
Why does the Power LED not function with the QIAxcel or QIAxcel Advanced instrument plugged in and the main power switch turned on?
FAQ ID -1851
View
Why does the miScript Target Protector not induce the degradation process?
FAQ ID -2263
View
Why does the DNeasy mericon Food Kit use a QIAquick column and Buffer PB rather than a DNeasy column and Buffer AL, which might be expected since the kit isolates genomic DNA?
FAQ ID - 3347
View
Why does the DNeasy mericon Food extraction kit use a QIAquick column and not DNeasy column?
FAQ ID -3148
View
Why does the copy function not apply relative to migration time and normalized area values after loading the DNA size marker table and adding the alignment marker peaks on the QIAxcel System?
FAQ ID -1832
View
Why does the connection test fail?
FAQ ID - 141522
View
Why does the Allprep DNA/RNA 96 Kit use Buffer RLT, whereas the AllPrep DNA/RNA Mini Kit uses Buffer RLT Plus?
FAQ ID -1999
View
Why does TA/UA cloning work with your proofreading HotStar HiFidelity DNA Polymerase?
FAQ ID -1053
View
Why does my realtime PCR assay quality decrease over time?
FAQ ID -589
View
Why does my purified DNA/RNA sometimes have a 260/280 or 260/230 ratio of more than 2? Does that mean the purity is poor?
FAQ ID -3132
View
Why does my PCR product show up later when comparing the QuantiTect SYBR Green PCR Kits with Roche kits using the same annealing temperature?
FAQ ID -1083
View
Why does my isolated RNA have a low OD 260/280 ratio?
FAQ ID -97
View
Why does my DNA sample float out of the slot when loading it onto an agarose gel?
FAQ ID -205
View
Why does an intensity calibration of the gel cartridge for the QIAxcel System and QIAxcel Advanced need to be performed?
FAQ ID -1824
View
Why does an intensity calibration of the gel cartridge for the QIAxcel Connect need to be performed?
FAQ ID - 3858
View
Why does AllPrep DNA/RNA/Protein Mini kit use buffer RLT, but Allprep DNA/RNA Mini and Allprep DNA/RNA Micro kits use buffer RLT Plus? Are these buffers interchangeable?
FAQ ID - 3391
View
Why do you recommend using Triton X for the purification of 6xHis-tagged protein?
-100
View
Why do the Internal Control templates for extraction (Internal Control DNA or RNA [High conc.]) have a 10x higher concentration than the IC templates provided with the QuantiFast Pathogen PCR +IC Kit and the QuantiFast Pathogen RT-PCR +IC Kit?
FAQ ID -2603
View
Why do replicates in real-time PCR have different plateau heights?
FAQ ID -539
View
Why do QuantiFast SYBR Green PCR Kits require such a high primer concentration?
FAQ ID -1443
View
Why do qBiomarker Copy Number PCR Arrays have assays in quadruplicate?
FAQ ID — 3414
View
Why do pQE-T7 vectors of the QIAgenes E. coli system have an additional UAG amber stop codon?
FAQ ID -2043
View
Why do my qPCR amplification curves or plots decrease in fluorescence intensity after the saturation phase?
FAQ ID -2689
View
Why do melting temperatures differ between PCR fragments amplified with QIAGEN's QuantiTect SYBR Green PCR Kits and Roche Kits?
FAQ ID -1084
View
Why do I sometimes get light blue colonies when using the QIAGEN PCR Cloning Kit?
FAQ ID -603
View
Why do I see multiple high-intensity peaks in my qPCR dissociation curve at temperatures less than 70 ºC?
FAQ ID -2690
View
Why do I see low, poor, or sub-standard amplification efficiency in my qRT-PCR assay?
FAQ ID -2695
View
Why do I observe a CT shift for the Internal Control in some samples in comparison to the CT value for the Internal Control of the dilution series of the Male Control DNA M1?
FAQ ID - 3745
View
Why do I need to identify my real-time instrument model when placing my order for RT² qPCR Primer Assays?
FAQ ID -2713
View
Why do I need normalization using Rotor-Gene ScreenClust HRM Software?
FAQ ID -2198
View
Why do I have wavy DNA bands on my agarose gel?
FAQ ID -2260
View
Why do I have to use an ACS (Assay Control Set)?
FAQ ID -2933
View
Why do I have to add beta-mercaptoethanol (beta-ME) to lysis Buffer RLT of the RNeasy Kits?
FAQ ID -101
View
Why do I get smeared PCR products?
FAQ ID -87
View
Why do I get genomic DNA contamination in my plasmid prep?
FAQ ID -353
View
Why do I get amplification in a negative control DNA tube using the REPLI-g Kit for WGA?
FAQ ID -675
View
Why do DNA samples appear in only a few channels when the alignment marker is working using the QIAxcel System or QIAxcel Advanced?
FAQ ID -1844
View
Why do casework-sample protocols for the QIAsymphony DNA Investigator Kit start with different lysate volumes (200 µl, 500 µl, or 1000 µl)?
FAQ ID -2035
View
Why did you choose ORF1a+ORF1b and ORF3a+ORF7a?
FAQ ID - 3893
View
Why did you choose MAPK1 as a positive control in the QIAGEN RNAi Human/Mouse Control Kit?
FAQ ID -604
View
Why did we not choose commonly used genes such as the S gene, E gene, or RdRP?
FAQ ID - 3892
View
Why did the real-time PCR yield Ct values < 12?
FAQ ID -2727
View
Why can’t I see my QIAcube Connect instrument in the QIAcube Connect App?
FAQ ID - 1414518
View
Why can't I find the DNA methylation qPCR Assay for my gene of interest?
FAQ ID -2743
View
Why can I not find the QuantiTect Primer Assay in GeneGlobe that I had previously ordered from QIAGEN?
FAQ ID -1138
View
Why can I not find the Q-Base device in the device list during the first installation?
FAQ ID - 141516
View
Why are two reagents used in the PAXgene Tissue System?
FAQ ID -3022
View
Why are two reagents used in the PAXgene Tissue System?
FAQ ID - 3603
View
Why are there various different QuantiTect Primer Assays for my gene (previous versions, transcript variants)?
FAQ ID -1133
View
Why are there various different QuantiFast Probe Assays for my gene (previous versions, transcript variants)?
FAQ ID -2366
View
Why are there no peaks in all channels during a run with the QIAxcel System or QIAxcel Advanced??
FAQ ID -1847
View
Why are the RT² qPCR Primer Assays not designed to cross exon-intron junctions or boundaries?
FAQ ID -2710
View
Why are the QuantiTect and QuantiFast Multiplex PCR Kits limited to triplex real-time PCR on some cyclers?
FAQ ID -715
View
Why are the QuantiFast Probe Assays located on a single exon, thereby also detecting genomic DNA?
FAQ ID -2365
View
Why are the denaturation and annealing/extension times for QuantiFast Multiplex RT-PCR Kits much shorter than those for QuantiFast Multiplex PCR Kits?
FAQ ID -2147
View
Why are the centrifugation speeds for the QIAamp DNA Mini kit at 6000 x g? Can I spin at full speed?
FAQ ID -474
View
Why are some of my samples outside of the cluster using Rotor-Gene ScreenClust HRM Software?
FAQ ID -2204
View
Why are some of my RNAprotect-stabilized samples frozen at 0ºC while others are not?
FAQ ID -758
View
Why are samples centrifuged at 4°C after the addition of chloroform when using RNeasy Lipid Tissue Kits?
FAQ ID -533
View
Why are samples centrifuged at 4°C after addition of chloroform to the QIAzol lysates in following kits: RNeasy Lipid Tissue Mini/Midi kits, RNeasy Microarray Tissue Mini kit, RNeasy Plus Universal Mini/Midi kit, RNeasy 96 Universal Tissue kit, miRNeasy Mini kit, miRNeasy Micro kit, miRNeasy 96 kit and miRNeasy Serum/Plasma kit?
FAQ ID -2345
View
Why are PCR bands relatively weak and "smearing" but the alignment marker bands are sharp when running samples on the QIAxcel System and QIAxcel Advanced?
FAQ ID -1837
View
Why are not all applications/kits/protocol files visible in the run setup (QIAcube Connect App)?
FAQ ID - 1414520
View
Why are my realtime PCR amplification plots hook-shaped?
FAQ ID -587
View
Why are my qPCR Ct values too low (< 12) in my qRT-PCR Assay?
FAQ ID -2684
View
Why are my qPCR Ct values too high (> 35 or not detectable) in my qRT-PCR assay?
FAQ ID -2685
View
Why are my DV200 values of my RNA sample so low?
FAQ ID - 3957
View
Why are most of my samples outside of the cluster in supervised mode using Rotor-Gene ScreenClust HRM Software?
FAQ ID -2205
View
Who can help in case of issues with the third party instrument?
3799
View
Who can help if it is not clear if issues are caused by the third party instrument or by the chemistry?
3802
View
Which version of the EZ2 Connect can run the EZ2 RNA/miRNA Tissue/Cells Kit?
FAQ ID - 3851
View
Which version of the EZ2 Connect can run the EZ2 RNA FFPE Kit?
FAQ ID - 3940
View
Which version of the EZ2 Connect can run the EZ2 AllPrep DNA/RNA FFPE Kit?
FAQ ID - 3947
View
Which variants of the EasyXtal 15-well tool are available?
FAQ ID -2221
View
Which types and amounts of sample material can be processed for RNA extraction on the QIAsymphony SP?
FAQ ID -1937
View
Which tubes are recommended for the QIAsymphony sample rack
FAQ ID - 3653
View
Which transfection reagent is recommended for transfection of the miScript Target Protectors?
FAQ ID -2252
View
Which software updates are included in a Premium Cover Agreement for my QIAGEN Instrument?
FAQ ID -1336
View
Which SARS-CoV-2 targets can the SARS-CoV-2 Neo Kit detect?
FAQ ID - 3881
View
Which sample types can be processed using the QIAsymphony Virus Blood 200 protocol?
FAQ ID -2154
View
Which sample types can be processed using the QIAsymphony PAXgene Blood RNA Protocol?
FAQ ID -2981
View
Which sample types can be processed using the PAXgene Blood miRNA protocols?
FAQ ID - 3478
View
Which sample preparation method is recommended for QIAcuity dPCR?
3779
View
Which sample materials are CE-IVD validated for the RealStar Filovirus Screen?
FAQ ID - 3529
View
Which Rotor-Gene Q rotor should be used with the EGFR RGQ PCR kit?
FAQ ID -2315
View
Which resin is used in the QIAexpress Ni-NTA Fast Start Columns?
FAQ ID -836
View
Which regions are covered by the GR DNAseq V2 Panels (#181900)?
FAQ ID - 3688
View
Which real-time PCR kits are recommended downstream of the QuantiTect Whole Transcriptome Kit?
FAQ ID -1592
View
Which QuantiTect Primer Assay should I choose for my gene of interest?
FAQ ID -1135
View
Which Qproteome or Protein Fractionation Kits from QIAGEN are compatible with tissue samples?
FAQ ID -755
View
Which qPCR instrument should I use with your RT² qPCR Primer Assays?
FAQ ID -2714
View
Which QIAsymphony Virus/Bacteria Kit size should be used for each protocol?
FAQ ID -2038
View
Which QIAGEN plasmid preparation kits will contain LyseBlue Reagent?
FAQ ID -865
View
Which QIAGEN kit do you recommend for purifying plasmid DNA suitable for transfection of sensitive cells?
FAQ ID -1092
View
Which QIAGEN DNA extraction kits are compatible with the PAXgene Saliva Collector?
3821
View
Which QIAcube Connect standard protocol might be suitable to extract RNA from saliva or from a buccal cell pellet?
FAQ ID - 141508
View
Which PyroMark Gold Q96 Reagent should be used for which instrument and application?
FAQ ID -2836
View
Which purity grade is recommended for pyrosequencing primers?
FAQ ID -2832
View
Which purification kits are recommended for the Pyro kits?
FAQ ID -2380
View
Which protocol line should I use to process plasma samples?
FAQ ID - 3654
View
Which probe labels are available for the QuantiFast Probe Assays?
FAQ ID -2361
View
Which probe labels are available for the Microbial DNA qPCR Assays?
FAQ ID — 3407
View
Which primers can I use for sequencing pQE-expression vector constructs?
FAQ ID -343
View
Which positive control should I use?
FAQ ID - 3896
View
Which plate formats are offered for QuantiTect Primer Assay Plates?
FAQ ID -2106
View
Which plasma volumes can I process with the QIAsymphony PAXgene Blood ccfDNA protocols?
FAQ ID - 3648
View
Which parts are exchanged during a Preventive Maintenance visit for my QIAGEN BioRobot?
FAQ ID -1334
View
Which operating system is compatible with PyroMark Q24 Advanced application software?
FAQ ID -3164
View
Which operating system is compatible with PyroMark IdentiFire Software?
FAQ ID - 3340
View
Which nucleotide analogs can be used with TopTaq DNA Polymerase?
FAQ ID -1741
View
Which network settings are supported by the QIAcube Connect and Q-Base?
FAQ ID - 141517
View
Which mode should I use in the Rotor-Gene ScreenClust HRM Software, supervised or unsupervised?
FAQ ID -2202
View
Which Microbial qPCR Mastermix should I use?
FAQ ID — 3395
View
Which kits can be used in combination with the PyroMark CpG Assays and PyroMark Custom Assays?
FAQ ID -2822
View
Which kits and protocols can be used to isolate nucleic acids from microdissected PAXgene Tissue fixed, paraffin-embedded (PFPE) and PAXgene Tissue fixed, cryo-embedded (PFCE) specimens?
FAQ ID - 3617
View
Which kit, the QIAprep 96 Plus Miniprep Kit or the QIAGEN Plasmid Plus 96 Miniprep Kit, do you recommend for transfection?
FAQ ID -2320
View
Which kit size should be used for each QIAsymphony DSP Virus/Pathogen protocol?
FAQ ID -2930
View
Which kit should I use for RNA isolation from Cartilage?
FAQ ID -1026
View
Which kit should be used to extract RNA from adipose tissue, brain, and other fatty animal tissues?
FAQ ID -467
View
Which kit can be used for simultaneous purification of DNA and RNA from biopsies?
FAQ ID -1089
View
Which kind of 96 and 384-well plates are available for FlexiPlate siRNAs?
FAQ ID -1459
View
Which is the best condition for eluting GST-tagged proteins from Glutathione Superflow?
FAQ ID -2173
View
Which human target is used for the quantification in the Investigator Quantiplex Kit?
FAQ ID -2577
View
Which homogenization method do you recommend for tissues stored in Allprotect Tissue Reagent?
FAQ ID -1572
View
Which heating block is recommended for the pyrosequencing annealing step?
FAQ ID -2838
View
Which fraction will contain soluble mitochondrial proteins using the Qproteome Cell Compartment Kit?
FAQ ID -839
View
Which fraction will contain endosomal, microsomal and lysosomal proteins using the Qproteome Cell Compartment Kit?
FAQ ID -808
View
Which formats are available for FlexiPlate siRNA?
FAQ ID -1358
View
Which fixation method is used in the PAXgene Tissue System?
FAQ ID - 3600
View
Which extraction kits are recommended?
FAQ ID - 3874
View
Which expression systems can be used with the QIAgenes Insect/Mammalia expression constructs?
FAQ ID -2049
View
Which EpiTect Bisulfite Plus kit do you recommend for blood samples, cells, and tissues?
FAQ ID -2411
View
Which end of the PCR primer for pyrosequencing should be biotinylated?
FAQ ID -2839
View
Which elution volumes can be used for the QIAsymphony DSP Virus/Pathogen protocols?
FAQ ID -2938
View
Which elution volumes can be selected using the QIAsymphony Virus Blood 200 protocol?
FAQ ID -2160
View
Which elution volumes are available with the QIAsymphony PAXgene Blood ccfDNA Kit?
FAQ ID - 3655
View
Which downstream applications have been tested with SARS-CoV-2-derived RNA purified from saliva collected into PAXgene Saliva Collector?
3828
View
Which downstream applications have been tested with DNA purified from samples collected in PAXgene Blood DNA Tubes IVD?
FAQ ID - 3512
View
Which downstream applications have been tested with DNA purified from saliva collected into PAXgene Saliva Collector?
3827
View
Which downstream applications have been tested with ccfDNA purified from whole blood collected and processed with the PAXgene Blood ccfDNA System?
FAQ ID - 3658
View
Which DNA/RNA extraction kits were tested in combination with the QuantiFast Pathogen PCR +IC Kit and the QuantiFast Pathogen RT-PCR +IC Kit?
FAQ ID -2606
View
Which deparaffinization method can I use with the AllPrep DNA/RNA FFPE Kit?
FAQ ID -2352
View
Which density gel type is contained in the yellow or green MaXtract Tubes?
FAQ ID -1315
View
Which database versions are used for CRISPR-Q Custom PCR Assay and CRISPR-Q Sanger Primers design?
3788
View
Which crystallization solutions are compatible with lipidic cubic phase crystallization?
FAQ ID -2418
View
Which controls can I choose for FlexiPlate siRNA?
FAQ ID -1368
View
Which conditions are recommended for the storage of tissues in PAXgene Tissue STABILIZER?
FAQ ID - 3605
View
Which competent E. coli cells should I use to amplify the SureSilencing shRNA Plasmids?
FAQ ID -2890
View
Which chloroform should I use for the RNeasy Lipid Tissue Kit?
FAQ ID -516
View
Which chemical products do you use to prepare screening solutions for protein crystallization?
FAQ ID -1122
View
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