常见问题

 

您无权限查看此资源

What sample numbers can be processed on the TissueLyser LT?
FAQ ID -2547
What size bead should I use for tougher samples with the TissueLyser LT?
FAQ ID -2554
When performing the Internal Control assay for the QuantiFast Pathogen PCR +IC Kit and the QuantiFast Pathogen RT-PCR +IC Kit, what channel or filter can be used to detect MAX?
FAQ ID -2597
Why is the fluorescence signal of the Internal Control on my Rotor-Gene Q very low?
FAQ ID -2574
Can specific types of white blood cells be enriched from a PAXgene Bone Marrow RNA Tube prior to the RNA isolation?
FAQ ID -2508
Can bone marrow aliquots other than 2 mL be pipetted into the PAXgene Bone Marrow RNA Tube?
FAQ ID -2503
Are the Internal Controls used in the QuantiFast Pathogen PCR +IC Kit and the QuantiFast Pathogen RT-PCR +IC Kit homologous to a known target?
FAQ ID -2598
Can I use water to elute the RNA in the PAXgene Bone Marrow RNA Kit?
FAQ ID -2512
Can PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue be used for in situ hybridization?
FAQ ID -2529
Can bone marrow from leukemia patients with elevated numbers of white blood cells be processed with the PAXgene Bone Marrow RNA System?
FAQ ID -2510
Can HiPerFect HTS be used for miRNA?
FAQ ID -2561
Can HiPerFect HTS Reagent be used for transfecting shRNAs?
FAQ ID -2559
How long does the sample disruption with the TissueLyser LT take?
FAQ ID -2550
How many times can the Internal Controls used in the QuantiFast Pathogen PCR +IC Kit and the QuantiFast Pathogen RT-PCR +IC Kit be freeze-thawed?
FAQ ID -2599
How long can I store the dilutions of the Control DNA Z1 standards at 2°C?
-8
How long can primer and probes from the mericon Assay kits be kept at°C once reconstituted?
-20
How should the PAXgene Bone Marrow RNA Tubes be frozen after collection and for how long?
FAQ ID -2505
I routinely heat an aliquot of the RNA eluate prior to my RT reaction. Do I still need to heat the eluate after the PAXgene Bone Marrow RNA preparation?
FAQ ID -2513
How much time may the aspirated bone marrow sample be allowed to incubate before pipetting it into the PAXgene Bone Marrow RNA Tube?
FAQ ID -2504
Do you have FACS or Fluoro-Microscopy data using HiPerFect HTS Reagent for delivery of siRNA?
FAQ ID -2557
How is stability and performance of these mericon Assay kits guaranteed?
FAQ ID -2542
How are the supernatants resulting from the first two spin steps of the PAXgene Bone Marrow RNA kit purification procedure disposed?
FAQ ID -2516
What are typical yields of bisulfite converted DNA when using the EpiTect Plus Bisulfite Kits?
FAQ ID -2408
What batch sizes can be selected with the PAXgene Blood RNA MDx Kit?
FAQ ID -2491
What are the labels of the probe which is used in the Internal Control Assay for detection of the IC?
FAQ ID -2449
What is the detection limit for the QuantiFast Pathogen + IC kits?
FAQ ID -2453
In which solution can I keep my protein for the NexTal Cubic Phase kits?
FAQ ID -2416
What is the difference between QuantiTect Virus Kit and the new QuantiFast Pathogen +IC Kits?
FAQ ID -2448
Is there a critical step during upfront sample handling when using the PAXgene Blood RNA MDX kit?
FAQ ID -2493
Which EpiTect Bisulfite Plus kit do you recommend for blood samples, cells, and tissues?
FAQ ID -2411
What is the nature of the Internal Control in the QuantiFast Pathogen + IC kit?
FAQ ID -2450
Where can I find general information about RT-PCR and PCR arrays such as information about sample preparation, reverse transcription, quantitative PCR, real-time PCR, and PCR arrays?
FAQ ID -2446
What is the maximum number of batches for the QIAsymphony PAXgene Blood RNA protocol for a single run?
FAQ ID -2498
Why should I work with diluted or undiluted screens?
FAQ ID -2419
Why should I use the EpiTect Plus FFPE Bisulfite Kit instead of isolating FFPE DNA first and then doing the bisulfite conversion?
FAQ ID -2412
What is the difference between sponge, cubic and lamellar phase of the NeXtal CubicPhase crystallisation?
FAQ ID -2420
Where are the somatic mutations published?
FAQ ID -2429
What should be the concentration of my protein for the Nextal Cubic Phase products?
FAQ ID -2417
What kind of control samples should I use for the particular iPSC-qBiomarker Arrays I have ordered?
FAQ ID -2441
What is the range indicated by 'Std. Error' on 'Results' page?
FAQ ID -2456
Can the PAXgene Blood RNA MDx Kit be used with both the BioRobot Universal System and the BioRobot MDx?
FAQ ID -2489
Can the Internal Controls be ordered separately in the QuantiFast Pathogen + IC kit for use during purification?
FAQ ID -2451
Can the PAXgene Blood RNA MDx Kit be used for diagnostic or prognostic procedures?
FAQ ID -2488
Can the QIAsymphony PAXgene Blood RNA System be used for diagnostic or prognostic procedures?
FAQ ID -2496
Can the PAXgene Blood miRNA System be used for diagnostic or prognostic procedures?
FAQ ID -2494
At what temperature can I perform the NexTal Cubic Phase experiment and incubate the plate?
FAQ ID -2415
How are the different mutations for a single gene normalized on the qBiomarker Somatic Mutation PCR array?
FAQ ID -2428
How can I check DNA sample quality before using the Somatic Mutation PCR Arrays & Assays?
FAQ ID -2430
How much DNA starting sample is needed for analysis with a Somatic Mutations PCR Array? And Assay?
FAQ ID -2431
How do I get started using the NeXtal CubicPhase kits?
FAQ ID -2413
How pure should my protein be with the NexTal CubicPhase kits?
FAQ ID -2414
How many samples can be processed with the QIAsymphony PAXgene Blood RNA Kit?
FAQ ID -2497
How many samples can be processed with the PAXgene Blood RNA MDx Kit?
FAQ ID -2490
In the REST software, what is the range indicated by 'Std. Error' on the 'Results' page?
FAQ ID -2455
How many PAXgene Blood RNA Tubes can be processed per PAXgene 96 Blood RNA Kit well plate?
FAQ ID -2485
If less than 48/96 samples are being processed, is it possible to leave positions empty with the PAXgene Blood RNA MDx Kit?
FAQ ID -2492
How does the DNA Protection work in the EpitTect Bisulfite kits?
FAQ ID -2410
How is the hypothesis test performed in the REST software?
FAQ ID -2459
How do I determine if my gene expression results are statistically significant in the REST software?
FAQ ID -2458
How many different genes and gene mutations are on a Somatic Mutation PCR Array?
FAQ ID -2427
How long does a QuantiFast Pathogen + IC run take on the Rotor-Gene Q?
FAQ ID -2452
How do I know in which phase of my NeXtal CubicPhase cristallisation experiment I am?
FAQ ID -2421
How great is the enrichment of miRNA when using the dedicated PAXgene Blood miRNA Kit compared to the PAXgene Blood RNA Kit?
FAQ ID -2495
How do I harvest the crystals from meso phase? Are special tools needed?
FAQ ID -2422
Why are two reagents used in the PAXgene Tissue System?
FAQ ID -3022
What is the maximum size of tissue that can be fixed in a PAXgene Tissue FIX Container (50 ml)?
FAQ ID -3024
5. What is the maximum size of the tissue sample that can be fixed in a PAXgene Tissue Container?
FAQ ID -3023
What happens if a tissue sample larger than 20 x 20 x 20 mm is used for fixation with the PAXgene Tissue FIX Container (50 ml)?
FAQ ID -3025
How long is the fixation time when using PAXgene Tissue FIX Container?
FAQ ID -3026
Is it possible to fix over a weekend?
FAQ ID -3027
How long can a tissue specimen stay in the PAXgene Tissue STABILIZER?
FAQ ID -3028
What is the minimum incubation time required when using the PAXgene Tissue STABILIZER?
FAQ ID -3029
Is it possible to integrate the PAXgene Tissue STABILIZER into the processing?
FAQ ID -3037
Is it possible to archive PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?
FAQ ID -3038
Is the morphology after H&E staining comparable to formalin-fixed samples?
FAQ ID -3039
Are immunohistochemistry (IHC) assays developed for formalin-fixed, paraffin-embedded (FFPE) compatible with PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?
FAQ ID -3040
Is it possible to use formalin-fixed, paraffin-embedded (FFPE) kits and protocols for extraction of biomolecules from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?
FAQ ID -3043
is the purity of nucleic acids isolated with the PAXgene Tissue Kits?
FAQ ID -3044
What is the integrity of RNA from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?
FAQ ID -3046
What is the average RNA yield from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?
FAQ ID -3045
What is the RT-PCR performance of RNA purified from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue compared to RNA from snap frozen or formalin-fixed, paraffin-embedded (FFPE) tissue?
FAQ ID -3050
What is the PCR performance of DNA purified from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue compared to DNA from snap frozen or formalin-fixed, paraffin-embedded (FFPE) tissue?
FAQ ID -3051
How can proteins purified from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue be analyzed?
FAQ ID -3052
What is the DNA integrity from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?
FAQ ID -3047
Does the PAXgene Tissue RNA Kit co-purify small RNAs?
FAQ ID -3048
How can I extract proteins from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?
FAQ ID -3049
Can PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue be used for in situ hybridization?
FAQ ID -3042
What are the typical yields obtained from Buccal swabs on BioSprint 96?
FAQ ID -3071
Can I use a USB drive to transfer protocols to BioSprint?
FAQ ID -3072
Is it possible to manually process PAXgene Tissue treated samples without using an automated processor?
FAQ ID -3036
Are PAXgene Tissue Containers suitable for long term-storage at freezing temperatures?
FAQ ID -3030
Is it possible to process formalin-fixed and PAXgene Tissue fixed samples together in one run?
FAQ ID -3033
Is it necessary to clean a processor normally used for formalin-fixed tissue before using it with PAXgene Tissue fixed tissue?
FAQ ID -3034
Is a special processing protocol needed?
FAQ ID -3035
Can sections of PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue be used for other histochemical staining techniques, such as PAS?
FAQ ID -3041
Are the multicopy reference assays for Type-it CNV PCR +qC Kits and the qBiomarker assays the same?
FAQ ID -3000
How do I clean up RNA preparations containing miRNA?
FAQ ID -3002
What is the fixation method used in the PAXgene Tissue System?
FAQ ID -3019
What does PAXgene Tissue FIX contain?
FAQ ID -3020
Can I perform the IDH 1/2 RGQ PCR test with slides containing less than 40% tumor cells?
FAQ ID -3098
Can the MaXtract High Density Tube be used with QIAzol to isolate RNA?
FAQ ID -1304
What volume of bacterial culture can be processed using the QuickLyse Miniprep Kit?
FAQ ID -1357
What factors determine if denatured proteins accumulate underneath, or inside the gel of the MaXtract Low and High Density Tubes?
FAQ ID -1312
I have a Human Druggable Genome siRNA Set V2.0. Can I reorder the siRNAs from this set?
FAQ ID -1366
If both MaXtract Low Density and High Density is suitable for an application, which type is preferable to use?
FAQ ID -1310
How will service work carried out on my QIAGEN BioRobot be documented?
FAQ ID -1331
If I cannot order FlexiPlate siRNA online, how do I order?
FAQ ID -1371
What information about my gene or siRNA do I need to order FlexiPlate siRNA?
FAQ ID -1365
Is it possible to use MaXtract for an organic extraction with a mixture of phenol and BCP (1 bromine chlorpropane)?
-3
What information is provided with FlexiPlate siRNAs?
FAQ ID -1363
Is it possible to elute plasmid DNA obtained with the QuickLyse Miniprep Kit in nuclease-free water?
FAQ ID -1355
Which formats are available for FlexiPlate siRNA?
FAQ ID -1358
When isolating DNA from plant cells using CTAB, should MaXtract Low or High Density be used?
FAQ ID -1308
Which parts are exchanged during a Preventive Maintenance visit for my QIAGEN BioRobot?
FAQ ID -1334
Which controls can I choose for FlexiPlate siRNA?
FAQ ID -1368
Which density gel type is contained in the yellow or green MaXtract Tubes?
FAQ ID -1315
What is the composition of FlexiPlate siRNA buffer?
FAQ ID -1361
Which software updates are included in a Premium Cover Agreement for my QIAGEN Instrument?
FAQ ID -1336
Can MaXtract tubes be autoclaved prior to use?
FAQ ID -1305
Can I reduce the amount of starting material below 10 ng when using the REPLI-g UltraFast Mini Kit?
FAQ ID -1325
Can I reduce the reaction time to e.g., 30 minutes, when using the REPLI-g UltraFast Mini Kit?
FAQ ID -1326
Can I use any type of DNA extraction method to purify template DNA for amplification using the REPLI-g Mitochondrial DNA Kit?
FAQ ID -1321
Can several extraction steps be performed in the same MaXtract tube?
FAQ ID -1302
Can a phenol-chloroform ratio different from 1:1 also be used with MaXtract Low and High Density?
FAQ ID -1309
Can DNA isolated with MaXtract tubes be used for downstream applications such as restriction digestion, labeling, blotting, PCR, and automated sequencing?
FAQ ID -1303
Can I amplify mitochondrial DNA directly from whole blood or cells using the REPLI-g Mitochondrial DNA Kit?
FAQ ID -1319
Can I order Alexa Fluor labeled controls with FlexiPlate siRNA?
FAQ ID -1369
Can HP Validated siRNAs be ordered on the FlexiPlate?
FAQ ID -1367
Can frozen sample material pulverized in liquid nitrogen be directly added to MaXtract tubes?
FAQ ID -1301
Can I also screen large constructs like BACs, fosmids, and cosmids using the QIAGEN PlasmidAmp Kit?
FAQ ID -1337
How much REPLI-g amplified mitochondrial DNA should be used in a subsequent PCR reaction?
FAQ ID -1320
Do mutations in the target mitochondrial DNA prevent primer binding, resulting in no DNA amplification with the REPLI-g Mitochondrial DNA Kit?
FAQ ID -1323
Do you have stability data for genomic DNA isolated with the BioSprint Kits?
FAQ ID -1318
Do you have stability data for genomic DNA isolated with the Flexigene DNA Kit?
FAQ ID -1317
Does the addition of sample and organic solution to the MaXtract Tubes require a specific pipetting sequence?
FAQ ID -1307
How can I upload my gene list for FlexiPlate siRNA to GeneGlobe?
FAQ ID -1364
How does a Service Support Agreement provide defined budgeting for maintaining my BioRobot?
FAQ ID -1330
How can I ensure optimal performance of my QIAGEN instrument?
FAQ ID -1329
Is it possible to purify viral RNA using FastLane Cell RT-PCR Kits?
FAQ ID -1484
Why is the activation time for HotStarTaq Plus Polymerase in the QuantiFast SYBR Green Kits different from that for QuantiFast Probe Kits?
FAQ ID -1449
Why is the RT step with the QuantiFast RT Kits much shorter compared to QuantiTect RT Kits?
FAQ ID -1451
Why is the storage time for QuantiFast PCR Kits shorter than that for QuantiTect PCR Kits?
FAQ ID -1446
Why is the reaction volume for QuantiFast PCR Kits lower than that for QuantiTect PCR Kits?
FAQ ID -1447
Why is the QuantiFast denaturation step different for PCR and RT-PCR runs in the two-step protocol for the ABI 7500 and other cyclers?
FAQ ID -1442
What is the minimum number of siRNAs per order? If I order 40 siRNAs, can I get those on 2 plates?
FAQ ID -1468
Can purification columns from other suppliers be processed on the QIAcube?
FAQ ID -1406
Can QIAcube protocol runs be interrupted, and subsequently be continued to completion?
FAQ ID -1414
Can I use more than 4 µl FastLane lysate in subsequent real-time RT-PCR?
FAQ ID -1480
Are FlexiPlate siRNAs available at a higher final concentration than 10 µM?
FAQ ID -1476
Can different amounts of siRNA be ordered on one FlexiPlate?
FAQ ID -1473
Can 2 µl reaction volumes be used with QuantiFast PCR Kits?
FAQ ID -1440
Do the QuantiTect Primer Assays work with QuantiFast SYBR Green Kits?
FAQ ID -1439
Do special settings have to be used for QuantiFast PCR Kits on the Eppendorf Mastercycler ep realplex?
FAQ ID -1437
What information is required to reorder specific siRNAs from a siRNA Set in FlexiPlate format?
FAQ ID -1463
What are the turnaround times for FlexiPlate siRNA orders?
FAQ ID -1478
How much time will be saved when switching from standard cycling to fast cycling with QuantiFast Kits?
FAQ ID -1438
How many reactions can I perform with the new QuantiFast Kits for real-time PCR?
FAQ ID -1425
If FlexiPlate siRNA was accidentally redissolved in siRNA Suspension Buffer instead of water, resulting in a 2x buffer, would it affect performance of the siRNAs?
FAQ ID -1477
How do you achieve fast cycling, yet still deliver the same performance in PCR as that achieved with standard cycling?
FAQ ID -1430
Is it necessary to place the lids of the elution tube and column into the slots of the rotor adapter during processing on the QIAcube?
FAQ ID -1415
Why do QuantiFast SYBR Green PCR Kits require such a high primer concentration?
FAQ ID -1443
What QuantiFast Kit should be used on the Eppendorf Mastercycler ep realplex?
FAQ ID -1436
What is the detection limit of the QuantiFast Kits for real-time PCR?
FAQ ID -1432
Which kind of 96 and 384-well plates are available for FlexiPlate siRNAs?
FAQ ID -1459
Which cell lines have been tested with FastLane Cell RT-PCR Kits?
FAQ ID -1485
Can I adjust the ROX concentration in the QuantiFast master mix?
FAQ ID -1427
Can I follow the reaction setup for probe-based assays described in Appendix D of the FastLane Cell RT-PCR Handbook using 384-well plates?
FAQ ID -1482
Can FlexiPlate siRNAs be ordered in plates other than the standard NUNC 96 well V bottom plates?
FAQ ID -1458
Can fast cycling be carried out with QuantiFast Probe PCR Kits and FRET probes?
FAQ ID -1433
Can FlexiPlate siRNA be ordered in solution on dry-ice?
FAQ ID -1474
How do QuantiFast PCR Kits compare to QuantiTect PCR Kits for quantitative real-time PCR?
FAQ ID -1441
How can I get software updates for the QIAcube?
FAQ ID -1413
Do you offer trial-kit sizes for the new QuantiFast Kits?
FAQ ID -1429
Can the QIAcube rotor adapters be autoclaved?
FAQ ID -1409
Can QuantiFast PCR Kits be used on real-time PCR instruments without fast cycling options?
FAQ ID -1428
Does the high primer concentration required by QuantiFast SYBR Green PCR Kits impair annealing specificity?
FAQ ID -1444
Does the master mix in the QuantiFast Kits contain dUTP to allow UNG treatments?
FAQ ID -1431
Can the QuantiFast Probe PCR +ROX Vial mastermix be stored long-term after adding ROX dye?
FAQ ID -1435
What are important points regarding the genomic DNA wipeout buffer in the QuantiFast Probe RT-PCR Plus Kit?
FAQ ID -2359
Will the sequence of the QuantiFast Probe Assays be provided?
FAQ ID -2369
Why are samples centrifuged at 4°C after addition of chloroform to the QIAzol lysates in following kits: RNeasy Lipid Tissue Mini/Midi kits, RNeasy Microarray Tissue Mini kit, RNeasy Plus Universal Mini/Midi kit, RNeasy 96 Universal Tissue kit, miRNeasy Mini kit, miRNeasy Micro kit, miRNeasy 96 kit and miRNeasy Serum/Plasma kit?
FAQ ID -2345
Why are there various different QuantiFast Probe Assays for my gene (previous versions, transcript variants)?
FAQ ID -2366
Why are the QuantiFast Probe Assays located on a single exon, thereby also detecting genomic DNA?
FAQ ID -2365
What are the main differences between the qBiomarker PCR Arrays and the RT2 Profiler PCR Arrays?
FAQ ID -2438
What are the recommended storage conditions of the QuantiFast Probe RT-PCR Plus Kit components?
FAQ ID -2355
What are the differences between the existing QuantiFast Probe RT-PCR Kit and the new Plus Kit?
FAQ ID -2358
What is the concentration of the primers and the probe in a reaction using the QuantiFast Probe Assays?
FAQ ID -2370
What is the average length of a QuantiFast Probe Assay?
FAQ ID -2374
Is the RNeasy Plus Universal Mini Kit suited for adipose, brain, and other fatty tissues?
FAQ ID -2343
Is there a size limit to the protein I can crystallize with the NexTal Cubic Phase products?
FAQ ID -2331
Is the T790M mutation included in the EGFR PCR kit?
FAQ ID -2314
Is it necessary to perform calibration steps for the use of the MAX dye in duplex RT-PCR experiments on different cyclers?
FAQ ID -2373
Is there also a miRNeasy Plus Universal Kit available?
FAQ ID -2346
I use cultured cells as starting material. Can I also use the RNeasy Plus Universal Kit?
FAQ ID -2342
I would like to order the QuantiFast Probe Assays without master mix. Is this possible?
FAQ ID -2360
My ADAPT report states "Run Invalid" and does not display any sample results. Why is this?
FAQ ID -2306
Is V600E the only mutation that can be detected with the codon 600 assay in the BRAF Pyro Kit?
FAQ ID -2382
Is the yield and quality of RNA and DNA purified with the AllPrep DNA/RNA FFPE Kit comparable to yield and quality of nucleic acids purified separately with other methods.
FAQ ID -2349
Which purification kits are recommended for the Pyro kits?
FAQ ID -2380
Which kit, the QIAprep 96 Plus Miniprep Kit or the QIAGEN Plasmid Plus 96 Miniprep Kit, do you recommend for transfection?
FAQ ID -2320
Which probe labels are available for the QuantiFast Probe Assays?
FAQ ID -2361
What should be the concentration of my protein with the NexTal Cubic Phase products?
FAQ ID -2325
What is the maximum plasmid size which I can use with the QIAprep 96 Plus Miniprep Kit or the QIAGEN Plasmid Plus 96 Miniprep Kit?
FAQ ID -2316
What plug-ins are available for the Pyrosequencing kits?
FAQ ID -2381
Which crystallization solutions are compatible with lipidic cubic phase crystallization?
FAQ ID -2418
Which deparaffinization method can I use with the AllPrep DNA/RNA FFPE Kit?
FAQ ID -2352
What species are QuantiTect Probe Assays offered for?
FAQ ID -2367
Can the insertions in Exon 20 be detected with the EGFR Pyro Kit?
FAQ ID -2383
Is it also possible to isolate miRNA with the AllPrep DNA/RNA FFPE Kit?
FAQ ID -2348
Can I use the RNeasy Plus Universal Mini Kit for all types of tissue?
FAQ ID -2338
Can the QIAprep 96 Plus Miniprep Kit or the QIAGEN Plasmid Plus 96 Miniprep Kit be used for low-copy number plasmids?
FAQ ID -2317
Can the QuantiFast Probe Assay detect short fragmented RNA as well as longer fragments of RNA?
FAQ ID -2364
Can negative crossing threshold (CT) values be negative when using the KRAS and EGFR PCR kits?
FAQ ID -2300
Can QuantiFast Probe Assays be ordered offline via e-mail or fax?
FAQ ID -2368
For the the QIAprep 96 Plus Miniprep Kit or the QIAGEN Plasmid Plus 96 Miniprep Kit, can plates be partially used?
FAQ ID -2319
For which applications can the RNA isolated with the RNeasy Plus Universal Kit also be used?
FAQ ID -2339
Does the KRAS PCR kit cover the codon 61 mutation?
FAQ ID -2308
Are the QIAprep 96 Plus Miniprep Kit or the QIAGEN Plasmid Plus 96 Miniprep Kit suited for short hairpin vectors?
FAQ ID -2318
Are the QuantiFast Probe Assays hydrolysis or FRET probes?
FAQ ID -2363
Are the buffers in the RNeasy QIAcube kit delivered in QIAcube buffer bottles?
FAQ ID -2334
Are QIAshredder spin columns delivered with the RNeasy Mini QIAcube Kit?
FAQ ID -2333
Can I do singleplex PCR with the QuantiFast Probe Assays?
FAQ ID -2362
Can I use more than 10ng DNA for the PCR reaction for the Pyro kits?
FAQ ID -2379
Are the QuantiFast Probe Assays wet-lab validated?
FAQ ID -2375
At what temperature can I perform the experiment with the NexTal Cubic Phase kits?
FAQ ID -2323
How much can the mixed standard ?Ct values vary in the KRAS PCR kit?
FAQ ID -2307
How many samples can be processed per RNeasy Mini QIAcube kit?
FAQ ID -2332
How much DNA/RNA can be isolated with the AllPrep DNA/RNA FFPE Kit?
FAQ ID -2353
How many freeze-thaw cycles can the master mix contained in the QuantiFast Probe RT-PCR Plus Kit tolerate?
FAQ ID -2357
How pure should the chloroform used with the RNeasy Plus Universal Mini Kit be?
FAQ ID -2344
How much sample material can I use for DNA/RNA purification with the AllPrep DNA/RNA FFPE Kit?
FAQ ID -2351
How much time can be saved when using the RNeasy Mini QIAcube Kit?
FAQ ID -2335
How does the QuantiFast Probe RT-PCR Plus Kit eliminate genomic DNA contamination?
FAQ ID -2354
How do I analyze the data for the particular iPSC qBiomarker Arrays I have ordered?
FAQ ID -2440
How are the genes in each iPSC qBiomarker PCR Array selected?
FAQ ID -2439
How can I adjust the ROX concentration in the QuantiFast Probe RT-PCR Plus master mix?
FAQ ID -2356
How do I harvest the crystals from meso phase? Are special tools needed with the NexTal Cubic Phase products?
FAQ ID -2330
What is the advantage of EpiTect MethyLight PCR Kits over QuantiTect Probe PCR Kits for methylation studies?
FAQ ID -2010
What is the advantage of using the Type-it Microsatellite PCR Kit?
FAQ ID -2059
What is a good starting amount of template DNA for PCR with the EpiTect MSP Kit?
FAQ ID -2005
What is the advantage of using the Type-it Mutation Detect PCR Kit?
FAQ ID -2064
What is the maximum lifetime of the QIAGEN rotor for the Centrifuge 4 and 4K?
-16
What is the principle behind the DNA Polymerase in the EpiTect MSP Kit?
FAQ ID -2003
What is the maximum DNA yield from forensic samples processed with the QIAsymphony SP?
FAQ ID -2028
Is it possible to use different sample types in one run with the MagAttract 96 cador Pathogen Kit?
FAQ ID -2072
Is magnetic particle carry-over in the eluates on the BioSprint 96 normal?
FAQ ID -2073
What happens when using EpiTect MethyLight Assays on only partially bisulfite converted DNA template?
FAQ ID -2009
What are the expected PCR results when using EpiTect Control DNA on untreated or bisulfite-converted DNA?
FAQ ID -2011
What are the features of the expression vector used for QIAgenes Insect/Mammalia?
FAQ ID -2050
Why is CoralLoad included in the Type-it Mutation Detect, but not in the Type-it Microsatellite PCR Kit?
FAQ ID -2068
Why do casework-sample protocols for the QIAsymphony DNA Investigator Kit start with different lysate volumes (200 µl, 500 µl, or 1000 µl)?
FAQ ID -2035
Why do pQE-T7 vectors of the QIAgenes E. coli system have an additional UAG amber stop codon?
FAQ ID -2043
Why is the Type-it Mutation Detect PCR Kit recommended for preamplification of SNPs?
FAQ ID -2067
Why is maximum amplicon size for the Type-it Microsatellite PCR Kit limited to 500 bp?
FAQ ID -2060
Will the optimized DNA sequence for QIAgenes be provided?
FAQ ID -2045
What sample types can be processed using the QIAsymphony Virus/Bacteria protocols?
FAQ ID -2036
What sample volumes can be processed with the QIAsymphony Virus/Bacteria Kits?
FAQ ID -2037
Can data be analyzed on the Rotor-Gene Q while a run is in progress?
FAQ ID -2091
Are there recommendations for sensitive detection of underrepresented fragments using the Type-it Mutation Detect PCR Kit?
FAQ ID -2066
Are there two vectors for protein expression using QIAgenes in insect or mammalian cells?
FAQ ID -2048
Is mitochondrial DNA isolated with the QIAsymphony DNA Investigator Kit on the QIAsymphony SP?
FAQ ID -2030
Is it possible to edit the sample sheet in the Rotor-Gene Q software after a PCR run?
FAQ ID -2092
Is it possible to import a standard curve from a previous PCR run on the Rotor-Gene Q?
FAQ ID -2093
What kind of samples should be processed using the large-volume protocol with the QIAsymphony DNA Investigator Kit?
FAQ ID -2034
Which QIAsymphony Virus/Bacteria Kit size should be used for each protocol?
FAQ ID -2038
Which expression systems can be used with the QIAgenes Insect/Mammalia expression constructs?
FAQ ID -2049
What types of reaction vessels are required for use in the Rotor-Gene Q?
FAQ ID -2085
Which capillary sequencers can be used for detection of amplicons from the Type-it Microsatellite PCR Kits?
FAQ ID -2063
Can the EpiTect PCR Control DNAs be used for mouse and rat assays?
FAQ ID -2008
Can the EpiTect PCR Control DNAs also be used as standard in real-time methylation specific quantification?
FAQ ID -2006
Can the protocols for purification of small RNAs from cells in the appendices of the AllPrep DNA/RNA 96 Handbook also be used with tissue samples?
FAQ ID -2001
Can QIAGEN rotors used with the centrifuges 4C and 4KC also be used with 4-16 and 4-16K models?
-15
Can the MagAttract 96 cador Pathogen Kit also be used on the KingFisher 96 or MagMAX Express 96 instruments?
FAQ ID -2074
Can TopTaq Master Mix simply be stored at room temperature on the lab bench for up to 4 months?
FAQ ID -2052
Do all QIAsymphony DNA Investigator protocols use carrier RNA?
FAQ ID -2033
Can the Type-it Microsaltellite PCR Kit be used on cyclers with fast ramping rates?
FAQ ID -2062
Can the Type-it Mutation Detect PCR kit be used for multiplex PCR of fragments longer than 500 bp?
FAQ ID -2065
Can 0.1 ml and 0.2 ml tubes used on the Rotor-Gene Q be labeled?
FAQ ID -2082
Can DNase digestion be performed on the RNeasy 96 plate during the RNA purification part of the Allprep DNA/RNA 96 procedure?
FAQ ID -2000
How many signal readings are taken during the real-time data acquisition on the Rotor-Gene Q?
FAQ ID -2083
Can membrane proteins be expressed with QIAgenes expression kits?
FAQ ID -2047
Do QIAsymphony Virus/Bacteria samples require pretreatment before they are loaded onto the instrument?
FAQ ID -2039
Does the centrifugal force have any effect on the kinetics of a PCR reaction on the Rotor-Gene Q?
FAQ ID -2081
Does an internal control have to be included for each QIAsymphony Virus/Bacteria protocol run?
FAQ ID -2040
How can full-length genes or individual domains of the QIAgenes Insect/Mammalia constructs be subcloned?
FAQ ID -2051
Can frozen samples be used with the MagAttract 96 cador Pathogen Kit?
FAQ ID -2070
How fast does the rotor spin during a run on the Rotor-Gene Q?
FAQ ID -2080
How is the log graph threshold value (Ct) for the Rotor-Gene Q determined?
FAQ ID -2088
How long can DNA converted with EpiTect Bisulfite Kits be stored before use in methylation specific PCR with the EpiTect MSP Kit?
FAQ ID -2002
How will running less than 96 samples with the MagAttract 96 cador Pathogen kit affect usage of plasticware?
FAQ ID -2071
If new dyes or chemistries appear on the real-time PCR market, will Rotor-Gene Q be capable of running those dyes?
FAQ ID -2087
If orders cannot be placed online, how can QIAgenes be ordered?
FAQ ID -2046
How much space does the PyroMark Q24 instrument need?
FAQ ID -2098
Does the Type-it Microsatellite PCR Kit always require an annealing temperature of 60°C, or can pre-optimized annealing temperatures for target-primer systems be used?
FAQ ID -2061
What is the turn around time for the ordering of miScript Target Protector?
FAQ ID -2254
What is the optimal range of PCR fragment sizes for normalization with the SeqTarget System?
FAQ ID -2247
What is the processing time for 96 normalizations with the SeqTarget System?
FAQ ID -2237
What technologies are used by the KRAS and EGFR PCR kits?
FAQ ID -2286
What method do you recommend to quantify DNA before running the KRAS and EGFR PCR kit?
FAQ ID -2292
What kind of automation can be done with the EasyXtal 15-well Tool?
FAQ ID -2220
What format are the EasyXtal 15-well tools?
FAQ ID -2219
What dyes are used in the KRAS and EGFR PCR kits?
FAQ ID -2288
What do I do if I get amplification in my no template control (NTC) with the KRAS and EGFR PCR kits?
FAQ ID -2298
What do I need to provide when ordering a miScript Target Protector?
FAQ ID -2251
What is the difference of the sequence of a miScript miRNA Inhibitor and miScript Target Protector?
FAQ ID -2253
What is a miScript Target Protector?
FAQ ID -2249
What is a miScript Precursor Assay?
FAQ ID -2214
Which transfection reagent is recommended for transfection of the miScript Target Protectors?
FAQ ID -2252
Which variants of the EasyXtal 15-well tool are available?
FAQ ID -2221
Why are most of my samples outside of the cluster in supervised mode using Rotor-Gene ScreenClust HRM Software?
FAQ ID -2205
Why are some of my samples outside of the cluster using Rotor-Gene ScreenClust HRM Software?
FAQ ID -2204
Why does the miScript Target Protector not induce the degradation process?
FAQ ID -2263
Why does the SeqTarget Prep Protocol require a 2-step procedure?
FAQ ID -2238
What will happen, if the normalized DNA for the SeqTarget System is eluted with Buffer EB instead of EB2?
FAQ ID -2242
When negative results are generated using the KRAS and EGFR PCR kits, do I rerun them?
FAQ ID -2294
What type of samples can I use for the KRAS PCR and EGFR kits?
FAQ ID -2293
What voltage should be used to run my agarose gel with the GelPilot Agarose?
FAQ ID -2261
Which mode should I use in the Rotor-Gene ScreenClust HRM Software, supervised or unsupervised?
FAQ ID -2202
Do the KRAS and EGFR PCR kits contain a passive reference dye?
FAQ ID -2295
Does the atypical look of the lyophilized FlexiTube siRNA Premix have a negative impact on performance?
FAQ ID -2267
Does the miScript Target Protector match the target sequence?
FAQ ID -2250
How do I store my FlexiTube siRNA Premix?
FAQ ID -2270
Do I have to order at least 4 FlexiTube siRNA Premixes?
FAQ ID -2266
How is ARMS technology superior to alternative methods such as dye terminator sequencing?
FAQ ID -2290
How do I search for all the miScript Target Protectors for one species?
FAQ ID -2255
How can I check the success of the normalization procedure for the SeqTarget System?
FAQ ID -2245
Can PCR fragments be normalized for the SeqTarget System after purification with QIAquick?
FAQ ID -2240
Are all 3 components of the SeqTarget System required or can a different Long Range PCR Kit and primers be used?
FAQ ID -2234
Can PCR fragments be normalized for the SeqTarget System without a previous purification step?
FAQ ID -2246
Can I use a 384 well plate for the KRAS and EGFR PCR kits?
FAQ ID -2296
Can the miScript Precursor Assay differentiate between pri- and pre-miRNA?
FAQ ID -2211
Does ethanol from the PCR product purification influence the normalization procedure for the SeqTarget System?
FAQ ID -2244
What are EasyXtal 15-well Tools used for?
FAQ ID -2217
What are Principal Components analyzed in Rotor-Gene ScreenClust HRM Software?
FAQ ID -2200
What are the amplicon lengths of the KRAS and EGFR PCR kits?
FAQ ID -2289
What are clusters analyzed in the Rotor-Gene ScreenClust HRM Software?
FAQ ID -2201
Is the SeqTarget System compatible with standard-size PCRs?
FAQ ID -2241
What controls are used in the KRAS and EGFR PCR kits to ensure reliable results?
FAQ ID -2291
What does QIAGEN use for a positive control in precursor detection experiments?
FAQ ID -2212
What do I do if I cannot see amplification in the FAM channel in my KRAS and EGFR PCR kits?
FAQ ID -2297
What are the corresponding QIAGEN names for former Biotage instruments?
FAQ ID -2285
What can be the reason for low DNA yields after normalization with the SeqTarget System?
FAQ ID -2239
How much buffer should be used for agarose gel electrophoresis?
FAQ ID -2257
How long does it take to generate results using the KRAS and EGFR PCR kits?
FAQ ID -2299
How should gels be cast with the GelPilot Agarose so that optimal resolution is achieved?
FAQ ID -2258
How long should PCR fragments be for the SeqTarget System?
FAQ ID -2236
Is it possible to use lower siRNA concentrations with FlexiTube siRNA Premix?
FAQ ID -2264
How many miScript Precursor Assays do you offer?
FAQ ID -2213
Is one able to use FlexiTube siRNA Premix for transfection of primary adherent cell types?
FAQ ID -2268
If I am working with a difficult-to-transfect cell type or if I obtain only weak silencing effects what can I do?
FAQ ID -2265
Is one able to use FlexiTube siRNA Premix for transfection of primary suspension cell types?
FAQ ID -2269
Is it possible to normalize to more or less than 500 ng with the SeqTarget System if needed?
FAQ ID -2235
How many samples can be processed with the QIAsymphony DNA Mini Kit and the Virus Blood 200 protocol?
FAQ ID -2159
How often can the Strep-Tactin Superflow Plus resin be reused?
FAQ ID -2169
Is it possible to purify and normalize long range PCRs produced with PCR kits from other vendors for the SeqTarget System?
FAQ ID -2243
If housekeeping and target genes differ significantly in abundance, will they both be amplified with equal efficiency using the Rotor-Gene Multiplex PCR Kit?
FAQ ID -2129
If Vapor-Lock is used to overlay PCR reactions, which volume should be entered as reaction volume in the Rotor-Gene Q Software?
FAQ ID -2150
Is it possible to purify a GST-tagged protein under denaturing conditions using Glutathione Superflow?
FAQ ID -2172
Is it normal that the binding capacity of GST-resins decreases with flow rate?
FAQ ID -2174
Why is the initial activation step different for Rotor-Gene Probe, SYBR Green and Multiplex Kits?
FAQ ID -2118
Will Rotor-Gene Kits also work on the Rotor-Gene 6000 and 3000 cyclers?
FAQ ID -2121
Why is there an asterisk for my miScript Primer Assays?
FAQ ID -2176
Will Uracil-N-Glycosylase (UNG) completely remove contaminating amplicons when using QuantiTect +UNG Kits?
FAQ ID -2139
Will QuantiTect Primer Assays work with Rotor-Gene SYBR Green Kits using an annealing step at 60ºC?
FAQ ID -2124
What is miRNA*? Do I need to consider this in my profiling experiment?
FAQ ID -2179
What are the main differences between Rotor-Gene and QuantiTect or QuantiFast PCR Kits?
FAQ ID -2119
What are possible reasons for reduced DNA yields with REPLI-g Kits?
FAQ ID -2148
What is the residuals plot in the Rotor-Gene ScreenClust HRM Software?
FAQ ID -2199
What is the target nucleic acid for the Virus Blood 200 Protocol on the QIAsymphony?
FAQ ID -2158
What is the detection limit of the Rotor-Gene and QuantiFast Multiplex RT-PCR Kits?
FAQ ID -2144
What is the minimum number of assays that can be ordered on QuantiTect Primer Assay Plates?
FAQ ID -2109
What is the maximum number of targets that can be amplified per reaction with the QuantiFast Multiplex RT-PCR Kit?
FAQ ID -2145
How should DNA isolated on the QIAsymphony be stored?
FAQ ID -2165
How should samples be stored before purification using the Virus Blood 200 Protocol on the QIAsymphony?
FAQ ID -2157
How pure should the chloroform used with the RNeasy Microarray Tissue Mini Kit be?
FAQ ID -2193
Why should I use the RNeasy Microarray Tissue Mini Kit when purifying RNA for microarray analysis?
FAQ ID -2188
Which elution volumes can be selected using the QIAsymphony Virus Blood 200 protocol?
FAQ ID -2160
What sample volumes can be used with the Virus Blood 200 Protocol on the QIAsymphony?
FAQ ID -2156
Which is the best condition for eluting GST-tagged proteins from Glutathione Superflow?
FAQ ID -2173
What species are QuantiTect Primer Assay Plates offered for?
FAQ ID -2110
Which plate formats are offered for QuantiTect Primer Assay Plates?
FAQ ID -2106
Why are the denaturation and annealing/extension times for QuantiFast Multiplex RT-PCR Kits much shorter than those for QuantiFast Multiplex PCR Kits?
FAQ ID -2147
Which sample types can be processed using the QIAsymphony Virus Blood 200 protocol?
FAQ ID -2154
How many reactions can be performed per well of the QuantiTect Primer Assay Plates?
FAQ ID -2107
Can assays for 20 and 100 reactions be ordered on the same QuantiTect Primer Assay Plate?
FAQ ID -2108
Can QuantiTect Primer Assay Plates be ordered offline via e-mail or fax?
FAQ ID -2112
Can Glutathione Superflow resin be reused?
FAQ ID -2171
Can Uracil-N-Glycosylase (UNG) be added to the QuantiTect PCR Master Mix and be stored for later use?
FAQ ID -2138
Can Uracil-N-Glycosylase (UNG) be purchased separately, independent of QuantiTect PCR Kits?
FAQ ID -2134
Are there any changes in the procedure using the Strep-Tactin Superflow Plus resin with the higher capacity over the standard matrix?
FAQ ID -2168
Can Uracil-N-Glycosylase (UNG) be used with a one-step RT-PCR kit, such as the QuantiTect Probe RT-PCR Kit?
FAQ ID -2135
Can the Rotor-Gene Multiplex RT-PCR Kit be used on other cyclers?
FAQ ID -2142
Can RNA isolated with the RNeasy Microarray Tissue Mini Kit also be used in downstream applications other than microarray analysis?
FAQ ID -2191
Are QuantiTect PCR +UNG Kits available in bulk format (e.g., 25 ml master mix)?
FAQ ID -2136
Are Rotor-Gene Kits compatible with reaction setup using the QIAgility instrument?
FAQ ID -2116
How does template quality influence the results with the Type-it HRM PCR Kit?
FAQ ID -2197
Are the layouts for both 96- and 384-well QuantiTect Primer Assay Plates visible when ordering online?
FAQ ID -2111
Do you have protocols for software setup on various PCR platforms using QuantiFast Probe RT-PCR Kits?
FAQ ID -2105
How does magnetic-particle carry-over in QIAsymphony eluates need to be handled?
FAQ ID -2166
How does the RNeasy Microarray Tissue Mini Kit prevent traces of phenol from negatively affecting the reverse-transcription step in microarray experiments?
FAQ ID -2189
How do Rotor-Gene Probe Kits compare with QuantiFast Probe Kits?
FAQ ID -2125
How many elution steps are recommended when using Glutathione Superflow resin?
FAQ ID -2175
Do you have a protocol for Rotor-Gene software setup for the Rotor-Gene SYBR Green PCR and RT-PCR Kits?
FAQ ID -2104
Does an internal control have to be used with the QIAsymphony Virus Blood 200 Protocol?
FAQ ID -2162
Can Vapor-Lock be used with samples processed on the QIAgility?
FAQ ID -2153
Do the master mixes in Rotor-Gene Kits contain dUTP to allow UNG pretreatment?
FAQ ID -2117
Do QuantiTect Primer Assays also work with Rotor-Gene SYBR Green PCR Kits?
FAQ ID -2123
Do limiting primer concentrations need to be determined when using the Rotor-Gene Multiplex PCR Kit?
FAQ ID -2128
What buffer should be used to dilute cDNA made using the miScript Reverse Transcription Kit?
FAQ ID -1601
Is FlexiTube GeneSolution available for other scales than the 1 nmol scale?
FAQ ID -1666
Is it necessary to clean up cDNA prepared with the QuantiTect Whole Transcriptome Kit?
FAQ ID -1610
Is the master mix of QuantiFast Kits for real-time PCR aliquoted into several tubes to prevent cross-contamination?
FAQ ID -1697
Is the QuantiTect Whole Transcriptome Kit suitable for miRNA amplification?
FAQ ID -1609
Is it necessary to include a genomic DNA removal step with the QuantiTect Whole Transcriptome Kit?
FAQ ID -1614
Is sequence information for HP Validated siRNAs provided in the FlexiTube format?
FAQ ID -1665
Where can I find background information and literature on Whole Genome Amplification with REPLI-g Kits?
FAQ ID -1690
Why is there DNA in the no-template (negative) control when using the QuantiTect Whole Transcriptome Kit?
FAQ ID -1620
What is the maximum volume of RNA in solution that can be used with the QuantiTect Whole Transcriptome Kit?
FAQ ID -1616
Can the Ni-NTA and Strep-Tactin Superflow Cartridges be reused?
FAQ ID -1607
Can the Ni-NTA Superflow Cartridges and/or Strep-Tactin Cartridges be connected in series?
FAQ ID -1606
Can the QuantiTect Whole Transcriptome Kit be used for amplification of RNA from LCM samples?
FAQ ID -1612
Can I store cDNA generated by the miScript System?
FAQ ID -1602
Does the QuantiTect Whole Transcriptome Kit work with RNA purified from bacteria, yeast, or plants?
FAQ ID -1615
Can T-Script® enzyme of the QuantiTect Whole Transcriptome Kit be substituted by Quantiscript Reverse Transcriptase?
FAQ ID -1617
Can the QuantiTect Whole Transcriptome reaction be stopped after the reverse transcription step to proceed with the ligation and amplification steps at a later time?
FAQ ID -1618
Can I still reorder siRNA that has been removed from the GeneGlobe data base?
FAQ ID -1668
Can amplification with the QuantiTect Whole Transcriptome Kit be extended to more than 8 hours to improve cDNA yields?
FAQ ID -1608
Can I select HP Validated and HP GenomeWide siRNAs for FlexiTube?
FAQ ID -1658
Can cDNA prepared with the QuantiTect Whole Transcriptome Kit be reampliied using REPLI-g Kits?
FAQ ID -1611
Can FlexiTube siRNA or FlexiTube GeneSolution products be ordered in solution?
FAQ ID -1667
How fast is the 6xHis-tagged protein purification process using Ni-NTA Superflow Cartridges?
FAQ ID -1605
How long is cDNA generated with the QuantiTect Whole Transcriptome Kit?
FAQ ID -1613
How long can I archive cDNA generated with the QuantiTect Whole Transcriptome Kit?
FAQ ID -1623
Do I need to use the miScript Universal Primer for detection of mRNA?
FAQ ID -1600
Have you observed co-amplification of genomic DNA from RNA templates used in the QuantiTect Whole Transcriptome Procedure?
FAQ ID -1619
Do you offer a QuantiFast Kit for one-step RT-PCR?
FAQ ID -1695
Do you have a protocol for purification of total RNA from plant latex?
FAQ ID -1694
Do you have a protocol for purification of total RNA from plant tissue with the RNeasy Lipid Tissue Mini Kit?
FAQ ID -1693
What effect does homogenization have on DNA yield and integrity when using AllPrep DNA/RNA Kits?
FAQ ID -1751
Is TopTaq DNA Polymerase a hot-start polymerase?
FAQ ID -1748
What is the fidelity of TopTaq DNA Polymerase?
FAQ ID -1739
Which nucleotide analogs can be used with TopTaq DNA Polymerase?
FAQ ID -1741
Why might Affymetrix GeneChip Mapping assays interfere with the REPLI-g FFPE kit?
FAQ ID -1755
What is the shelf-life of TopTaq DNA Polymerase and Master Mix?
FAQ ID -1743
What is the principle of the REPLI-g FFPE Kit?
FAQ ID -1753
What is the largest plasmid size that can be purified using the QuickLyse Miniprep Kit?
FAQ ID -1752
What is the minimum amount of starting material that can be used with the RNeasy Plus Micro and AllPrep DNA/RNA Micro Kit?
FAQ ID -1750
What is the recommended sample size for use with the REPLI-g FFPE Kit?
FAQ ID -1756
Can small miRNA-containing RNA fractions be separated from large RNAs using the miRNeasy FFPE Kit?
FAQ ID -1737
Can miRNA and other RNAs smaller than 200 nucleotides be isolated with the AllPrep DNA/RNA Micro Kit or RNeasy Plus Micro Kit?
FAQ ID -1749
Can TA/UA cloning be done with PCR products amplified with TopTaq DNA Polmyerase and TopTaq Master Mix Kits?
FAQ ID -1740
Are there problems with the default threshold setting on the Applied Biosystems 7500 when using QuantiFast Probe PCR Kits?
FAQ ID -1702
At which step in the REPLI-g FFPE protocol should PicoGreen quantification be done?
FAQ ID -1758
How can foaming of Buffer RLT Plus lysates be avoided when using RNeasy Plus Micro and Mini Kits, or the Allprep DNA/RNA Mini Kit?
FAQ ID -1734
Do you have protocols for sample processing on the Autopure LS instrument?
FAQ ID -1759
Have QuantiTect Primer Assays been tested with QuantiFast SYBR Green PCR Kits on the Mastercycler ep realplex?
FAQ ID -1714
Can the REPLI-g FFPE procedure restore DNA derived from FFPE tissue samples in the original order?
FAQ ID -1754
Can the REPLI-g FFPE Kit also be used for formalin-fixed, ethanol preserved samples?
FAQ ID -1760
Do you have a protocol for the isolation of total RNA from paraffin-embedded sections on the Biorobot M48?
FAQ ID -1733
Does TopTaq Stabilizer of the TopTaq DNA Polymerase and Master Mix Kits interfere with downstream applications?
FAQ ID -1744
Do you have a forensic post-PCR purification protocol to purify double-stranded DNA fragments from PCR reactions?
FAQ ID -1761
Is sample pretreatment required for purifying RNA on the QIAsymphony SP?
FAQ ID -1938
Is the QIAsymphony RNA Kit compatible with stabilized samples?
FAQ ID -1944
Is RNA co-purified with DNA using QIAsymphony DNA Tissue protocols?
FAQ ID -1920
Is there a charge for user training on the QIAsymphony SP System?
FAQ ID -1900
What are the expected DNA yields from tissue processed on the QIAsymphony SP?
FAQ ID -1919
QuantiTect Primer Assays are bioinformatically validated, genomewide primer sets. What does “bioinformatically validated” mean?
FAQ ID -1982
What is the range of elution volumes on the QIAsymphony SP?
FAQ ID -1908
How many samples can be processed per kit or per reagent cartridge for RNA purification on the QIAsymphony SP?
FAQ ID -1940
How does QIAsymphony SP keep cellular samples in suspension prior to pipetting so that samples do not settle?
FAQ ID -1905
How many PCR reactions can be performed for different scales of miScript Primer Assays?
FAQ ID -1990
How efficient is DNA removal by a gDNA Eliminator 96 plate compared with DNase digestion on an RNeasy 96 plate?
FAQ ID -1994
How long can homogenates remain on the QIAsymphony SP without risk of RNA degradation?
FAQ ID -1941
How much sample can be used with the Allprep DNA/RNA 96 Kit?
FAQ ID -1997
Which types and amounts of sample material can be processed for RNA extraction on the QIAsymphony SP?
FAQ ID -1937
How much starting material can be used with the QIAsymphony DNA Mini Kit on the QIAsymphony SP?
FAQ ID -1917
What sample types can be used with the AllPrep DNA/RNA 96 Kit?
FAQ ID -1996
What sample types should be processed with the High Content (HC) protocol for DNA extraction on the QIAsymphony SP?
FAQ ID -1918
What sample types can be used with the RNeasy Plus 96 Kit?
FAQ ID -1991
Will mitochondrial DNA also be isolated using the DNA Tissue protocols on the QIAsymphony SP?
FAQ ID -1921
Why does the Allprep DNA/RNA 96 Kit use Buffer RLT, whereas the AllPrep DNA/RNA Mini Kit uses Buffer RLT Plus?
FAQ ID -1999
Why is there no vacuum-only protocol for the RNeasy Plus 96 Kit?
FAQ ID -1993
What is the turn around time for custom miScript miRNA mimics, inhibitors and primer assays?
FAQ ID -1985
What kind of molecules are miScript miRNA Inhibitors?
FAQ ID -1983
Is it possible to modify QIAsymphony protocols?
FAQ ID -1903
What are the expected yields of total RNA isolated with the QIAsymphony SP?
FAQ ID -1942
What are the starting volumes of homogenate for RNA extraction on the QIAsymphony SP?
FAQ ID -1939
What sample input volumes can be used on the QIAsymphony SP?
FAQ ID -1907
What sample types can be processed using the tissue protocols for DNA extraction on the QIAsymphony SP?
FAQ ID -1915
What kind of molecules are miScript miRNA Mimics?
FAQ ID -1986
What RNA elution volumes are available with the QIAsymphony RNA Kit?
FAQ ID -1943
Do limiting primer concentrations need to be determined when using QuantiFast Multiplex PCR Kits?
FAQ ID -1976
Do samples require pretreatment before loading onto the QIAsymphony SP for DNA extraction?
FAQ ID -1916
Can uneven sample numbers be processed on the QIAsymphony SP module?
FAQ ID -1906
Does HiPerFect work for the transfection of miScript miRNA mimics and inhibitors?
FAQ ID -1984
Does the QIAsymphony SP system generate run reports and/or log files?
FAQ ID -1911
Do you have a protocol for the purification of archive-quality DNA from 100 to 5 x 10e8 cultured cells using Gentra Puregene Kits?
FAQ ID -1960
Do you have a protocol for the purification of archive-quality DNA from 0.5–20 mg paraffin-embedded tissue using Gentra Puregene Kits?
FAQ ID -1964
Do you have a protocol for the purification of archive-quality DNA from 1 ml of whole blood with the Gentra Puregene Tissue Kit?
FAQ ID -1953
Do you have a protocol for rapid purification of archive-quality DNA from up to 9 x 10e6 cells using Gentra Puregene Kits?
FAQ ID -1969
Can I program my own protocols for the QIAsymphony SP?
FAQ ID -1904
Are QuantiFast Multiplex PCR Kits available in trial sizes?
FAQ ID -1981
Can 4-plex, real-time PCR be performed on the ABI PRISM 7000, 7700, or 7900 using the QuantiFast Multiplex PCR Kit?
FAQ ID -1978
Can the QIAsymphony RNA Kit be used to purify RNA from blood?
FAQ ID -1945
Can the QIAsymphony SP Module be connected to a laboratory information management system (LIMS)?
FAQ ID -1909
Can protocol runs on the QIAsymphony SP be interrupted and resumed?
FAQ ID -1912
Can the protocols for purification of small RNAs from cells in the appendices of the RNeasy Plus 96 Handbook also be used with tissue samples?
FAQ ID -1995
Do you have a protocol for the purification of archive-quality DNA from marine invertebrate tissue using Gentra Puregene Kits?
FAQ ID -1950
Do you have a protocol for the purification of archive-quality DNA from nematodes using Gentra Puregene Kits?
FAQ ID -1951
Do you have a protocol for the purification of archive-quality DNA from human dried blood using Gentra Puregene Kits?
FAQ ID -1959
Do you have a protocol for the purification of archive-quality DNA from Gram-negative bacteria using Gentra Puregene Kits?
FAQ ID -1975
Do you have a protocol for the purification of archive-quality DNA from Gram-positive bacteria using Gentra Puregene Kits?
FAQ ID -1946
Do you have a protocol for the purification of archive-quality DNA from hair roots using Gentra Puregene Kits?
FAQ ID -1947
Do you have a protocol for the purification of DNA from fruit flies?
FAQ ID -1970
How can the QIAsymphony SP system be decontaminated?
FAQ ID -1914
Do you have a protocol for the purification of archive-quality DNA from tissue fixed in ethanol or formalin using Gentra Puregene Kits?
FAQ ID -1974
Do you have a protocol for the purification of archive-quality DNA from umbilical cord blood using Gentra Puregene Kits?
FAQ ID -1958
Do you have a protocol for the purification of archive-quality DNA from whole blood using Gentra Puregene Kits?
FAQ ID -1954
Do you have a protocol for the purification of archive-quality DNA from buffy coat using Gentra Puregene Kits?
FAQ ID -1955
Do you have a protocol for the purification of archive-quality DNA from cerebrospinal fluid (CSF) using Gentra Puregene Kits?
FAQ ID -1967
Do you have a protocol for the purification of archive-quality DNA from Chlamydomonas using Gentra Puregene Kits?
FAQ ID -1968
Do you have a protocol for the purification of archive-quality DNA from blood smears using Gentra Puregene Kits?
FAQ ID -1948
Do you have a protocol for the purification of archive-quality DNA from fecal cells using Gentra Puregene Kits?
FAQ ID -1972
Do you have a protocol for the purification of archive-quality DNA from buccal brushes using Gentra Puregene Kits?
FAQ ID -1965
Do you have a protocol for the purification of archive-quality DNA from fish using Gentra Puregene Kits?
FAQ ID -1973
Do you have a protocol for the purification of archive-quality DNA from Eimeria oocysts using Gentra Puregene Kits?
FAQ ID -1952
Do you have a protocol for the purification of archive-quality DNA from fungus using Gentra Puregene Kits?
FAQ ID -1971
Do you have a protocol for cleanup of REPLI-g amplified DNA?
FAQ ID -1545
What are the Tms of PCR products obtained using the miScript System?
FAQ ID -1599
What is Q-Bond used in the QIAGEN Fast Cycling PCR and QuantiFast Kits?
FAQ ID -1554
What controls should be used for normalizing miRNA real-time PCR data obtained with the miScript System?
FAQ ID -1596
Will QuantiTect Primer Assays work at an annealing temperature of 60ºC with QuantiFast SYBR Green PCR and RT-PCR Kits?
FAQ ID -1553
Which homogenization method do you recommend for tissues stored in Allprotect Tissue Reagent?
FAQ ID -1572
Can different incubation times be used for the 2 hour amplification step in the QuantiTect Whole Transcriptome protocol?
-8
Does residual Allprotect Tissue Reagent left behind on tissue samples interfere with DNA, RNA, and protein purification?
FAQ ID -1573
Is cDNA generated with the QuantiTect Whole Transcriptome Kit suitable for use in microarray analysis?
FAQ ID -1587
Do you have a protocol for purification of genomic DNA from cultured cells using the QIAamp DNA Micro Kit?
FAQ ID -1547
Do you have a protocol for concentration of RNA in 96-well plates?
FAQ ID -1546
Do you have a protocol for purification of total RNA from fatty tissues using QIAzol Lysis Reagent and MaXtract High Density?
FAQ ID -1550
Which real-time PCR kits are recommended downstream of the QuantiTect Whole Transcriptome Kit?
FAQ ID -1592
What primers are used in the reverse-transcription step of the QuantiTect Whole Transcriptome procedure?
FAQ ID -1584
Can the AllPrep DNA/RNA/Protein Mini Kit be used with fibrous or lipid tissues?
FAQ ID -1579
What is the maximum amount of RNA that can be used for amplification with the QuantiTect Whole Transcriptome Kit?
FAQ ID -1591
What starting amount of RNA purified using an miRNeasy Kit should be used with the miScript System?
FAQ ID -1597
Can RNA purified from formalin-fixed, paraffin-embedded (FFPE) tissue be used with the QuantiTect Whole Transcriptome Kit?
FAQ ID -1583
Can samples in Allprotect Tissue Reagent undergo repeated freeze-thaws without degradation of DNA, RNA, and protein?
FAQ ID -1575
Can the QuantiFast Probe PCR +ROX Vial Kit be used on the ABI PRISM 7900 and Applied Biosystems 7500 cyclers?
FAQ ID -1555
Does Buffer APP in the AllPrep DNA/RNA/Protein Mini Kit contain acetone?
FAQ ID -1576
Does cDNA amplified with the QuantiTect Whole Transcriptome Kit correspond to full-length RNA transcripts?
FAQ ID -1585
Can tissue incubated in Allprotect Tissue Reagent be used for morphological studies?
FAQ ID -1571
Can less than 10 ng RNA be used with the QuantiTect Whole Transcriptome Kit?
FAQ ID -1590
Can cDNA prepared by any method be used as starting material in the ligation reaction of the QuantiTect Whole Transcriptome Kit?
FAQ ID -1589
Can Allprotect Tissue Reagent be used to stabilize RNA, DNA and proteins in cells, bacteria, yeast, and plants?
FAQ ID -1574
Can degraded RNA be used with the QuantiTect Whole Transcriptome Kit?
FAQ ID -1586
How much cDNA is sufficient for real-time PCR detection of miRNA transcripts generated with the miScript System?
FAQ ID -1598
How many samples can be run in parallel with the Rotor-Gene Q real-time PCR instrument?
FAQ ID -1519
What main input voltage is required for the QIAxcel Analyzer?
FAQ ID -1821
Is there a quality difference between CompactPrep Plasmid Mega and Giga and the CompactPrep Plasmid Midi and Maxi Kits?
FAQ ID -1882
The Gel Cartridge is installed and all status indicators for the QIAxcel System are good but when clicking the "RUN" button, nothing happens. The green highlight goes down the Sequence?
FAQ ID -1850
Why does the copy function not apply relative to migration time and normalized area values after loading the DNA size marker table and adding the alignment marker peaks on the QIAxcel System?
FAQ ID -1832
Can customers use their own DNA size marker with the QIAxcel System and if yes, what concentration should be used?
FAQ ID -1841
Can data be saved at once with the QIAxcel System after analysis of all samples in a 96-well PCR plate?
FAQ ID -1861
How can a positive threshold be set when running the QIAxcel System?
FAQ ID -1843
How long will the intensity calibration procedure on the QIAxcel System take?
FAQ ID -1826
Will the QIAxcel System run using a green Data Review Software Key?
FAQ ID -1853
When should an intensity calibration be re-performed on the QIAxcel System?
FAQ ID -1825
Can the QIAxcel System or QIAxcel Advanced be connected to a network?
FAQ ID -1862
How can a clogged capillary channel be identified when running samples on the QIAxcel System or QIAxcel Advanced?
FAQ ID -1836
Can the QIAxcel software be loaded to another PC to view the data generated with the QIAxcel System?
FAQ ID -1855
Can multiple QIAxcel Systems be run from one computer?
FAQ ID -1860
How often should the solutions in the Buffer Tray for the QIAxcel System or QIAxcel Advanced be replaced?
FAQ ID -1840
How often should the alignment marker for the QIAxcel System be changed?
FAQ ID -1839
If the M500 method is used with the QIAxcel System, does a new DNA reference marker table have to be created?
FAQ ID -1856
In the results table generated with the QIAxcel System, what does "normalized area" mean?
FAQ ID -1854
What is the composition of buffer OCD?
FAQ ID -423
What is the composition of buffer QN?
FAQ ID -414
What is the composition of buffer OCL?
FAQ ID -422
What is the composition of buffer OL2?
FAQ ID -424
Can the QIAamp MinElute Virus Kits be used to extract viral nucleic acid from cell culture supernatant?
FAQ ID -472
Can RNAi experiments be performed in Drosophila?
FAQ ID -436
How is the RNeasy Lipid Tissue Mini Kit different from the RNeasy Mini Kit?
FAQ ID -468
Has RNAi been successful using siRNA in Zebrafish and Xenopus?
FAQ ID -400
Are Northern Blots sensitive enough to detect siRNA-induced gene silencing?
FAQ ID -403
Can I use the RNeasy MinElute Cleanup Kit to clean up my in vitro transcription reaction?
FAQ ID -429
What is the composition of buffer OEB?
FAQ ID -420
Why is the pQE DNA provided in QIAexpress Kits blue in color?
FAQ ID -487
What is the minimum elution volume when using the RNeasy Micro Kit?
FAQ ID -428
What is the maximum amount of starting material that can be processed with the RNeasy Micro Kit?
FAQ ID -426
What is the minimum elution volume when using the RNeasy MinElute Cleanup Kit?
FAQ ID -432
What is the smallest sample size that can be used with RNeasy Mini Kits?
FAQ ID -485
What is a homogeneous LiquiChip assay?
FAQ ID -476
What detection reagents (reporter fluorophores) do you recommend for the LiquiChip System?
FAQ ID -480
What are the excitation and emission wavelengths of the reporter and classification lasers of the LiquiChip Instrument?
FAQ ID -479
What is the binding capacity of the RNeasy MinElute Cleanup Kit column?
FAQ ID -430
What is the composition and size of the LiquiChip Beads?
FAQ ID -475
What is the binding capacity of the RNeasy Micro Kit column?
FAQ ID -427
Is dihydrofolate reductase (DHFR) immunogenic?
FAQ ID -471
What are some of the assays that are adaptable to the LiquiChip System?
FAQ ID -477
My QuantiTect Master Mix did not freeze at degrees. Is it still ok to use?
-20
I want to start with gene silencing (RNAi) experiments. Do you have informational literature?
FAQ ID -580
Is it possible to use less than 1 mg of protein with the PhosphoProtein Purification Kit?
FAQ ID -599
Is it possible to use a QIAzol lysate for the isolation of RNA from tissue on the BioRobot EZ1?
FAQ ID -573
What is a quenched FRET assay?
FAQ ID -542
What do you recommend for isolating RNA from more than 100 mg of plant tissue?
FAQ ID -570
What happens if I spin my lysate on the RNeasy Spin Columns at maximum speed?
FAQ ID -514
How should I dissolve the lyophilized Anti-His and Tag Antibodies?
-100
Is it possible to perform a UNG treatment when using QuantiTect kits?
FAQ ID -564
Is it possible to clean up a methylation reaction containing bisulfite with QIAquick Cleanup Kits?
FAQ ID -519
Is it possible to isolate both RNA and recombinant 6xHis-tagged protein from the same sample?
FAQ ID -532
Why are my realtime PCR amplification plots hook-shaped?
FAQ ID -587
Why are samples centrifuged at 4°C after the addition of chloroform when using RNeasy Lipid Tissue Kits?
FAQ ID -533
How was it determined that the PhosphoSerine Antibody will bind phosphoserines only?
FAQ ID -579
If pQE40 is used to express a fusion protein with DHFR, can the DHFR be cleaved afterwards?
FAQ ID -513
Which chloroform should I use for the RNeasy Lipid Tissue Kit?
FAQ ID -516
Will pancreas tissue swim on top of RNAprotect Tissue Reagent?
FAQ ID -536
Will the 2x QIAGEN Multiplex PCR Master Mix freeze at -20°C?
FAQ ID - 525
Why does my realtime PCR assay quality decrease over time?
FAQ ID -589
What is the longest fragment that was cloned with the pQE UA Cloning Vector?
-30
What is the difference between BSA-free and BSA-containing RGS-His Antibody?
FAQ ID -569
回到顶部