常见问题

 

您无权限查看此资源

Can QIAzol aqueous phase be used with the gDNA eliminator columns in RNeasy Plus mini kit?
FAQ ID -3151
What is in the HiSpec Buffer that selectively synthesizes cDNA from mature miRNAs ONLY?
FAQ ID -3168
What is the optimal DNA concentration for the Investigator ESSplex Plus Kit?
FAQ ID -2646
Why do I see low, poor, or sub-standard amplification efficiency in my qRT-PCR assay?
FAQ ID -2695
What is the difference between adding the Internal Control (IC) template used in the QuantiFast Pathogen PCR +IC Kit and the QuantiFast Pathogen RT-PCR +IC Kit to the amplification reaction versus adding the IC template at the extraction step?
FAQ ID -2600
Is the QuantiFast SYBR Green PCR Kit compatible with the ViiA7 cycler from Applied Biosystems?
FAQ ID -2652
What are the most reliable methods for preparing high-quality RNA from cell or tissue samples, for use in gene expression analysis experiments?
FAQ ID -2657
What should the cycler set-up be for a duplex reaction with the pathogen assay (FAM) and the Internal Control assay (MAX) on ABI instruments, when using the QuantiFast Pathogen PCR +IC Kit or the QuantiFast Pathogen RT-PCR +IC Kit?
FAQ ID -2610
What should the cycler set-up be for a duplex reaction with the pathogen assay (FAM) and the Internal Control assay (MAX) on Mx instruments from Stratagene (Agilent), when using the QuantiFast Pathogen PCR +IC Kit or the QuantiFast Pathogen RT-PCR +IC Kit?
FAQ ID -2609
What should I do if I suspect that my RNA preparation contains RNase contamination?
FAQ ID -2661
What real-time cycler should I use for my qPCR experiments?
FAQ ID -2670
Which DNA/RNA extraction kits were tested in combination with the QuantiFast Pathogen PCR +IC Kit and the QuantiFast Pathogen RT-PCR +IC Kit?
FAQ ID -2606
What should the Rotor-Gene Q cycler settings be for a duplex reaction with the pathogen assay (FAM) and the Internal Control assay (MAX), when using the QuantiFast Pathogen PCR +IC Kit or the QuantiFast Pathogen RT-PCR +IC Kit?
FAQ ID -2608
What testing should be performed to assess the quality of an RNA sample?
FAQ ID -2660
For the QuantiFast Pathogen RT-PCR +IC kit, does the Internal Control also control for the reverse transcription reaction?
FAQ ID -2601
Do we have data on long term stability and storage of DNA purified on BioRobot M48?
FAQ ID -2611
Following thermal cycling, reaction volume has evaporated from some of the wells of my qPCR assay plate. What should I do?
FAQ ID -2683
How can I predict the percent qPCR signal due to contaminating DNA, for a given qPCR assay, and its matching NRT control?
FAQ ID -2688
How can I ensure that reaction volume is not lost due to evaporation during thermal cycling?
FAQ ID -2679
How can I determine whether amplification occurs from mRNA-derived cDNA or from genomic DNA contamination?
FAQ ID -2687
Can the Internal Control DNA or RNA be added directly to the sample?
FAQ ID -2602
Do the QuantiFast Pathogen PCR +IC Kit and the QuantiFast Pathogen RT-PCR +IC Kit contain ROX in the master mix?
FAQ ID -2605
Are primers available that only detect mitochondrial DNA encoded genes and not nuclear genomic DNA encoded genes?
FAQ ID -2680
Do I need to run a standard curve before the actual PCR array experiment?
FAQ ID -2664
Does the amount of Internal Control to be spiked into the lysis buffer or lysate depend on the elution volume only?
FAQ ID -2604
May I try the data analysis tool without using your PCR array kit?
FAQ ID -2698
Is the QuantiFast Multiplex PCR +R Kit compatible with the ViiA7 cycler from Applied Biosystems?
FAQ ID -2653
Is the QuantiFast Probe PCR +ROX Vial Kit compatible with the ViiA7 cycler from Applied Biosystems?
FAQ ID -2651
Is it good to pool multiple RNA replicates to detect expression changes that are consistently reproducible?
FAQ ID -2663
How important is the RNA purification process, for obtaining reliable qRT-PCR results?
FAQ ID -2655
Is a special processing protocol needed?
FAQ ID -2523
Is it possible to archive PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue blocks?
FAQ ID -2524
The disruption of my sample worked well with the TissueLyser LT, but I am not satisfied with the RNA yield. What do you suggest?
FAQ ID -2555
What are the dimensions of the TissueLyser LT?
FAQ ID -2545
What is the average RNA yield from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?
FAQ ID -2534
On which cyclers can the QuantiFast Pathogen PCR +IC Kit or the QuantiFast Pathogen RT-PCR +IC Kit be used?
FAQ ID -2596
What are the storage conditions for the mericon Assay kits?
FAQ ID -2540
What is in the elution buffer in the PAXgene Bone Marrow RNA kit?
FAQ ID -2511
Is it possible to microdissect PAXgene Tissue fixed, paraffin-embedded (PFPE) and PAXgene Tissue fixed, cryo-embedded (PFCE) tissues?
FAQ ID -2531
Is the performance of the HiPerFect HTS Reagent affected if it has accidentally been stored at°C?
-20
Is it possible to embed PAXgene Tissue fixed and stabilized samples in Optimal Cutting Temperature (OCT) medium for freezing?
FAQ ID -2525
Is the morphology after H&E staining comparable to formalin-fixed samples?
FAQ ID -2526
What RNA yields are expected with the PAXgene Bone Marrow RNA System?
FAQ ID -2506
What is the maximum tissue size that can be fixed in a PAXgene Tissue Container?
FAQ ID -2518
What is the RT-PCR performance of RNA purified from PAXgene Tissue fixed, paraffin-embedded (PFPE) and PAXgene Tissue fixed, cryo-embedded (PFCE) tissue compared to RNA from snap frozen or formalin-fixed, paraffin-embedded (FFPE) tissue?
FAQ ID -2538
What is the expected amplicon size when using the mericon Assay kits?
FAQ ID -2544
What is the typical amount of tissue used for disruption with the TissueLyser LT?
FAQ ID -2552
What is the longest period you recommend to leave out the dilute HiPerFect HTS at RT before use?
FAQ ID -2560
What kind of sample tubes are recommended to use with the TissueLyser LT?
FAQ ID -2553
What is the purity of nucleic acids extracted with the PAXgene Tissue kits?
FAQ ID -2533
Can the PAXgene Bone Marrow RNA System be used to isolate genomic DNA or viral RNA and DNA?
FAQ ID -2507
Can the PAXgene Bone Marrow RNA System be used with animal bone marrow or with blood samples?
FAQ ID -2501
Can the PAXgene RNA stabilization solution/bone marrow mixture from the PAXgene Bone Marrow RNA Tube be disposed of by adding bleach?
FAQ ID -2515
Can sections of PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue be used for other histochemical staining techniques, such as PAS?
FAQ ID -2530
Can the PAXgene Bone Marrow RNA System be used for other human body fluids than bone marrow samples or cultured cells of human origin?
FAQ ID -2502
Can the sample tubes and the TissueLyser LT Adapter be cooled with liquid nitrogen?
FAQ ID -2549
Can the TissueLyser LT Adapter be removed for cleaning?
FAQ ID -2546
Can the samples on the TissueLyser LT be protected from degradation of nucleic acids or proteins?
FAQ ID -2548
When using the QuantiFast Pathogen PCR +IC Kit or the QuantiFast Pathogen RT-PCR +IC Kit, what should the fluorescent label of the probe be for the customer-defined assay to detect the pathogen?
FAQ ID -2594
When using HiPerFect HTS Reagent is it necessary to optimize amount of siRNA and transfection reagent?
FAQ ID -2556
What sample numbers can be processed on the TissueLyser LT?
FAQ ID -2547
What size bead should I use for tougher samples with the TissueLyser LT?
FAQ ID -2554
When performing the Internal Control assay for the QuantiFast Pathogen PCR +IC Kit and the QuantiFast Pathogen RT-PCR +IC Kit, what channel or filter can be used to detect MAX?
FAQ ID -2597
Why is the fluorescence signal of the Internal Control on my Rotor-Gene Q very low?
FAQ ID -2574
Can specific types of white blood cells be enriched from a PAXgene Bone Marrow RNA Tube prior to the RNA isolation?
FAQ ID -2508
Can bone marrow aliquots other than 2 mL be pipetted into the PAXgene Bone Marrow RNA Tube?
FAQ ID -2503
Are the Internal Controls used in the QuantiFast Pathogen PCR +IC Kit and the QuantiFast Pathogen RT-PCR +IC Kit homologous to a known target?
FAQ ID -2598
Can I use water to elute the RNA in the PAXgene Bone Marrow RNA Kit?
FAQ ID -2512
Can PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue be used for in situ hybridization?
FAQ ID -2529
Can bone marrow from leukemia patients with elevated numbers of white blood cells be processed with the PAXgene Bone Marrow RNA System?
FAQ ID -2510
Can HiPerFect HTS be used for miRNA?
FAQ ID -2561
Can HiPerFect HTS Reagent be used for transfecting shRNAs?
FAQ ID -2559
How long does the sample disruption with the TissueLyser LT take?
FAQ ID -2550
How many times can the Internal Controls used in the QuantiFast Pathogen PCR +IC Kit and the QuantiFast Pathogen RT-PCR +IC Kit be freeze-thawed?
FAQ ID -2599
How long can I store the dilutions of the Control DNA Z1 standards at 2°C?
-8
How long can primer and probes from the mericon Assay kits be kept at°C once reconstituted?
-20
How should the PAXgene Bone Marrow RNA Tubes be frozen after collection and for how long?
FAQ ID -2505
I routinely heat an aliquot of the RNA eluate prior to my RT reaction. Do I still need to heat the eluate after the PAXgene Bone Marrow RNA preparation?
FAQ ID -2513
How much time may the aspirated bone marrow sample be allowed to incubate before pipetting it into the PAXgene Bone Marrow RNA Tube?
FAQ ID -2504
Do you have FACS or Fluoro-Microscopy data using HiPerFect HTS Reagent for delivery of siRNA?
FAQ ID -2557
How is stability and performance of these mericon Assay kits guaranteed?
FAQ ID -2542
How are the supernatants resulting from the first two spin steps of the PAXgene Bone Marrow RNA kit purification procedure disposed?
FAQ ID -2516
What are typical yields of bisulfite converted DNA when using the EpiTect Plus Bisulfite Kits?
FAQ ID -2408
What batch sizes can be selected with the PAXgene Blood RNA MDx Kit?
FAQ ID -2491
What are the labels of the probe which is used in the Internal Control Assay for detection of the IC?
FAQ ID -2449
What is the detection limit for the QuantiFast Pathogen + IC kits?
FAQ ID -2453
In which solution can I keep my protein for the NexTal Cubic Phase kits?
FAQ ID -2416
What is the difference between QuantiTect Virus Kit and the new QuantiFast Pathogen +IC Kits?
FAQ ID -2448
Is there a critical step during upfront sample handling when using the PAXgene Blood RNA MDX kit?
FAQ ID -2493
Which EpiTect Bisulfite Plus kit do you recommend for blood samples, cells, and tissues?
FAQ ID -2411
What is the nature of the Internal Control in the QuantiFast Pathogen + IC kit?
FAQ ID -2450
Where can I find general information about RT-PCR and PCR arrays such as information about sample preparation, reverse transcription, quantitative PCR, real-time PCR, and PCR arrays?
FAQ ID -2446
What is the maximum number of batches for the QIAsymphony PAXgene Blood RNA protocol for a single run?
FAQ ID -2498
Why should I work with diluted or undiluted screens?
FAQ ID -2419
Why should I use the EpiTect Plus FFPE Bisulfite Kit instead of isolating FFPE DNA first and then doing the bisulfite conversion?
FAQ ID -2412
What is the difference between sponge, cubic and lamellar phase of the NeXtal CubicPhase crystallisation?
FAQ ID -2420
Where are the somatic mutations published?
FAQ ID -2429
What should be the concentration of my protein for the Nextal Cubic Phase products?
FAQ ID -2417
What kind of control samples should I use for the particular iPSC-qBiomarker Arrays I have ordered?
FAQ ID -2441
What is the range indicated by 'Std. Error' on 'Results' page?
FAQ ID -2456
Can the PAXgene Blood RNA MDx Kit be used with both the BioRobot Universal System and the BioRobot MDx?
FAQ ID -2489
Can the Internal Controls be ordered separately in the QuantiFast Pathogen + IC kit for use during purification?
FAQ ID -2451
Can the PAXgene Blood RNA MDx Kit be used for diagnostic or prognostic procedures?
FAQ ID -2488
Can the QIAsymphony PAXgene Blood RNA System be used for diagnostic or prognostic procedures?
FAQ ID -2496
Can the PAXgene Blood miRNA System be used for diagnostic or prognostic procedures?
FAQ ID -2494
At what temperature can I perform the NexTal Cubic Phase experiment and incubate the plate?
FAQ ID -2415
How are the different mutations for a single gene normalized on the qBiomarker Somatic Mutation PCR array?
FAQ ID -2428
How can I check DNA sample quality before using the Somatic Mutation PCR Arrays & Assays?
FAQ ID -2430
How much DNA starting sample is needed for analysis with a Somatic Mutations PCR Array? And Assay?
FAQ ID -2431
How do I get started using the NeXtal CubicPhase kits?
FAQ ID -2413
How pure should my protein be with the NexTal CubicPhase kits?
FAQ ID -2414
How many samples can be processed with the QIAsymphony PAXgene Blood RNA Kit?
FAQ ID -2497
How many samples can be processed with the PAXgene Blood RNA MDx Kit?
FAQ ID -2490
In the REST software, what is the range indicated by 'Std. Error' on the 'Results' page?
FAQ ID -2455
How many PAXgene Blood RNA Tubes can be processed per PAXgene 96 Blood RNA Kit well plate?
FAQ ID -2485
If less than 48/96 samples are being processed, is it possible to leave positions empty with the PAXgene Blood RNA MDx Kit?
FAQ ID -2492
How does the DNA Protection work in the EpitTect Bisulfite kits?
FAQ ID -2410
How is the hypothesis test performed in the REST software?
FAQ ID -2459
How do I determine if my gene expression results are statistically significant in the REST software?
FAQ ID -2458
How many different genes and gene mutations are on a Somatic Mutation PCR Array?
FAQ ID -2427
How long does a QuantiFast Pathogen + IC run take on the Rotor-Gene Q?
FAQ ID -2452
How do I know in which phase of my NeXtal CubicPhase cristallisation experiment I am?
FAQ ID -2421
How great is the enrichment of miRNA when using the dedicated PAXgene Blood miRNA Kit compared to the PAXgene Blood RNA Kit?
FAQ ID -2495
How do I harvest the crystals from meso phase? Are special tools needed?
FAQ ID -2422
Why are two reagents used in the PAXgene Tissue System?
FAQ ID -3022
What is the maximum size of tissue that can be fixed in a PAXgene Tissue FIX Container (50 ml)?
FAQ ID -3024
5. What is the maximum size of the tissue sample that can be fixed in a PAXgene Tissue Container?
FAQ ID -3023
What happens if a tissue sample larger than 20 x 20 x 20 mm is used for fixation with the PAXgene Tissue FIX Container (50 ml)?
FAQ ID -3025
How long is the fixation time when using PAXgene Tissue FIX Container?
FAQ ID -3026
Is it possible to fix over a weekend?
FAQ ID -3027
How long can a tissue specimen stay in the PAXgene Tissue STABILIZER?
FAQ ID -3028
What is the minimum incubation time required when using the PAXgene Tissue STABILIZER?
FAQ ID -3029
Is it possible to integrate the PAXgene Tissue STABILIZER into the processing?
FAQ ID -3037
Is it possible to archive PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?
FAQ ID -3038
Is the morphology after H&E staining comparable to formalin-fixed samples?
FAQ ID -3039
Are immunohistochemistry (IHC) assays developed for formalin-fixed, paraffin-embedded (FFPE) compatible with PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?
FAQ ID -3040
Is it possible to use formalin-fixed, paraffin-embedded (FFPE) kits and protocols for extraction of biomolecules from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?
FAQ ID -3043
is the purity of nucleic acids isolated with the PAXgene Tissue Kits?
FAQ ID -3044
What is the integrity of RNA from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?
FAQ ID -3046
What is the average RNA yield from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?
FAQ ID -3045
What is the RT-PCR performance of RNA purified from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue compared to RNA from snap frozen or formalin-fixed, paraffin-embedded (FFPE) tissue?
FAQ ID -3050
What is the PCR performance of DNA purified from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue compared to DNA from snap frozen or formalin-fixed, paraffin-embedded (FFPE) tissue?
FAQ ID -3051
How can proteins purified from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue be analyzed?
FAQ ID -3052
What is the DNA integrity from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?
FAQ ID -3047
Does the PAXgene Tissue RNA Kit co-purify small RNAs?
FAQ ID -3048
How can I extract proteins from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?
FAQ ID -3049
Can PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue be used for in situ hybridization?
FAQ ID -3042
What are the typical yields obtained from Buccal swabs on BioSprint 96?
FAQ ID -3071
Can I use a USB drive to transfer protocols to BioSprint?
FAQ ID -3072
Is it possible to manually process PAXgene Tissue treated samples without using an automated processor?
FAQ ID -3036
Are PAXgene Tissue Containers suitable for long term-storage at freezing temperatures?
FAQ ID -3030
Is it possible to process formalin-fixed and PAXgene Tissue fixed samples together in one run?
FAQ ID -3033
Is it necessary to clean a processor normally used for formalin-fixed tissue before using it with PAXgene Tissue fixed tissue?
FAQ ID -3034
Is a special processing protocol needed?
FAQ ID -3035
Can sections of PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue be used for other histochemical staining techniques, such as PAS?
FAQ ID -3041
Are the multicopy reference assays for Type-it CNV PCR +qC Kits and the qBiomarker assays the same?
FAQ ID -3000
How do I clean up RNA preparations containing miRNA?
FAQ ID -3002
What is the fixation method used in the PAXgene Tissue System?
FAQ ID -3019
What does PAXgene Tissue FIX contain?
FAQ ID -3020
Can I perform the IDH 1/2 RGQ PCR test with slides containing less than 40% tumor cells?
FAQ ID -3098
Can the MaXtract High Density Tube be used with QIAzol to isolate RNA?
FAQ ID -1304
What volume of bacterial culture can be processed using the QuickLyse Miniprep Kit?
FAQ ID -1357
What factors determine if denatured proteins accumulate underneath, or inside the gel of the MaXtract Low and High Density Tubes?
FAQ ID -1312
I have a Human Druggable Genome siRNA Set V2.0. Can I reorder the siRNAs from this set?
FAQ ID -1366
If both MaXtract Low Density and High Density is suitable for an application, which type is preferable to use?
FAQ ID -1310
How will service work carried out on my QIAGEN BioRobot be documented?
FAQ ID -1331
If I cannot order FlexiPlate siRNA online, how do I order?
FAQ ID -1371
What information about my gene or siRNA do I need to order FlexiPlate siRNA?
FAQ ID -1365
Is it possible to use MaXtract for an organic extraction with a mixture of phenol and BCP (1 bromine chlorpropane)?
-3
What information is provided with FlexiPlate siRNAs?
FAQ ID -1363
Is it possible to elute plasmid DNA obtained with the QuickLyse Miniprep Kit in nuclease-free water?
FAQ ID -1355
Which formats are available for FlexiPlate siRNA?
FAQ ID -1358
When isolating DNA from plant cells using CTAB, should MaXtract Low or High Density be used?
FAQ ID -1308
Which parts are exchanged during a Preventive Maintenance visit for my QIAGEN BioRobot?
FAQ ID -1334
Which controls can I choose for FlexiPlate siRNA?
FAQ ID -1368
Which density gel type is contained in the yellow or green MaXtract Tubes?
FAQ ID -1315
What is the composition of FlexiPlate siRNA buffer?
FAQ ID -1361
Which software updates are included in a Premium Cover Agreement for my QIAGEN Instrument?
FAQ ID -1336
Can MaXtract tubes be autoclaved prior to use?
FAQ ID -1305
Can I reduce the amount of starting material below 10 ng when using the REPLI-g UltraFast Mini Kit?
FAQ ID -1325
Can I reduce the reaction time to e.g., 30 minutes, when using the REPLI-g UltraFast Mini Kit?
FAQ ID -1326
Can I use any type of DNA extraction method to purify template DNA for amplification using the REPLI-g Mitochondrial DNA Kit?
FAQ ID -1321
Can several extraction steps be performed in the same MaXtract tube?
FAQ ID -1302
Can a phenol-chloroform ratio different from 1:1 also be used with MaXtract Low and High Density?
FAQ ID -1309
Can DNA isolated with MaXtract tubes be used for downstream applications such as restriction digestion, labeling, blotting, PCR, and automated sequencing?
FAQ ID -1303
Can I amplify mitochondrial DNA directly from whole blood or cells using the REPLI-g Mitochondrial DNA Kit?
FAQ ID -1319
Can I order Alexa Fluor labeled controls with FlexiPlate siRNA?
FAQ ID -1369
Can HP Validated siRNAs be ordered on the FlexiPlate?
FAQ ID -1367
Can frozen sample material pulverized in liquid nitrogen be directly added to MaXtract tubes?
FAQ ID -1301
Can I also screen large constructs like BACs, fosmids, and cosmids using the QIAGEN PlasmidAmp Kit?
FAQ ID -1337
How much REPLI-g amplified mitochondrial DNA should be used in a subsequent PCR reaction?
FAQ ID -1320
Do mutations in the target mitochondrial DNA prevent primer binding, resulting in no DNA amplification with the REPLI-g Mitochondrial DNA Kit?
FAQ ID -1323
Do you have stability data for genomic DNA isolated with the BioSprint Kits?
FAQ ID -1318
Do you have stability data for genomic DNA isolated with the Flexigene DNA Kit?
FAQ ID -1317
Does the addition of sample and organic solution to the MaXtract Tubes require a specific pipetting sequence?
FAQ ID -1307
How can I upload my gene list for FlexiPlate siRNA to GeneGlobe?
FAQ ID -1364
How does a Service Support Agreement provide defined budgeting for maintaining my BioRobot?
FAQ ID -1330
How can I ensure optimal performance of my QIAGEN instrument?
FAQ ID -1329
Is it possible to purify viral RNA using FastLane Cell RT-PCR Kits?
FAQ ID -1484
Why is the activation time for HotStarTaq Plus Polymerase in the QuantiFast SYBR Green Kits different from that for QuantiFast Probe Kits?
FAQ ID -1449
Why is the RT step with the QuantiFast RT Kits much shorter compared to QuantiTect RT Kits?
FAQ ID -1451
Why is the storage time for QuantiFast PCR Kits shorter than that for QuantiTect PCR Kits?
FAQ ID -1446
Why is the reaction volume for QuantiFast PCR Kits lower than that for QuantiTect PCR Kits?
FAQ ID -1447
Why is the QuantiFast denaturation step different for PCR and RT-PCR runs in the two-step protocol for the ABI 7500 and other cyclers?
FAQ ID -1442
What is the minimum number of siRNAs per order? If I order 40 siRNAs, can I get those on 2 plates?
FAQ ID -1468
Can purification columns from other suppliers be processed on the QIAcube?
FAQ ID -1406
Can QIAcube protocol runs be interrupted, and subsequently be continued to completion?
FAQ ID -1414
Can I use more than 4 µl FastLane lysate in subsequent real-time RT-PCR?
FAQ ID -1480
Are FlexiPlate siRNAs available at a higher final concentration than 10 µM?
FAQ ID -1476
Can different amounts of siRNA be ordered on one FlexiPlate?
FAQ ID -1473
Can 2 µl reaction volumes be used with QuantiFast PCR Kits?
FAQ ID -1440
Do the QuantiTect Primer Assays work with QuantiFast SYBR Green Kits?
FAQ ID -1439
Do special settings have to be used for QuantiFast PCR Kits on the Eppendorf Mastercycler ep realplex?
FAQ ID -1437
What information is required to reorder specific siRNAs from a siRNA Set in FlexiPlate format?
FAQ ID -1463
What are the turnaround times for FlexiPlate siRNA orders?
FAQ ID -1478
How much time will be saved when switching from standard cycling to fast cycling with QuantiFast Kits?
FAQ ID -1438
How many reactions can I perform with the new QuantiFast Kits for real-time PCR?
FAQ ID -1425
If FlexiPlate siRNA was accidentally redissolved in siRNA Suspension Buffer instead of water, resulting in a 2x buffer, would it affect performance of the siRNAs?
FAQ ID -1477
How do you achieve fast cycling, yet still deliver the same performance in PCR as that achieved with standard cycling?
FAQ ID -1430
Is it necessary to place the lids of the elution tube and column into the slots of the rotor adapter during processing on the QIAcube?
FAQ ID -1415
Why do QuantiFast SYBR Green PCR Kits require such a high primer concentration?
FAQ ID -1443
What QuantiFast Kit should be used on the Eppendorf Mastercycler ep realplex?
FAQ ID -1436
What is the detection limit of the QuantiFast Kits for real-time PCR?
FAQ ID -1432
Which kind of 96 and 384-well plates are available for FlexiPlate siRNAs?
FAQ ID -1459
Which cell lines have been tested with FastLane Cell RT-PCR Kits?
FAQ ID -1485
Can I adjust the ROX concentration in the QuantiFast master mix?
FAQ ID -1427
Can I follow the reaction setup for probe-based assays described in Appendix D of the FastLane Cell RT-PCR Handbook using 384-well plates?
FAQ ID -1482
Can FlexiPlate siRNAs be ordered in plates other than the standard NUNC 96 well V bottom plates?
FAQ ID -1458
Can fast cycling be carried out with QuantiFast Probe PCR Kits and FRET probes?
FAQ ID -1433
Can FlexiPlate siRNA be ordered in solution on dry-ice?
FAQ ID -1474
How do QuantiFast PCR Kits compare to QuantiTect PCR Kits for quantitative real-time PCR?
FAQ ID -1441
How can I get software updates for the QIAcube?
FAQ ID -1413
Do you offer trial-kit sizes for the new QuantiFast Kits?
FAQ ID -1429
Can the QIAcube rotor adapters be autoclaved?
FAQ ID -1409
Can QuantiFast PCR Kits be used on real-time PCR instruments without fast cycling options?
FAQ ID -1428
Does the high primer concentration required by QuantiFast SYBR Green PCR Kits impair annealing specificity?
FAQ ID -1444
Does the master mix in the QuantiFast Kits contain dUTP to allow UNG treatments?
FAQ ID -1431
Can the QuantiFast Probe PCR +ROX Vial mastermix be stored long-term after adding ROX dye?
FAQ ID -1435
What are important points regarding the genomic DNA wipeout buffer in the QuantiFast Probe RT-PCR Plus Kit?
FAQ ID -2359
Will the sequence of the QuantiFast Probe Assays be provided?
FAQ ID -2369
Why are samples centrifuged at 4°C after addition of chloroform to the QIAzol lysates in following kits: RNeasy Lipid Tissue Mini/Midi kits, RNeasy Microarray Tissue Mini kit, RNeasy Plus Universal Mini/Midi kit, RNeasy 96 Universal Tissue kit, miRNeasy Mini kit, miRNeasy Micro kit, miRNeasy 96 kit and miRNeasy Serum/Plasma kit?
FAQ ID -2345
Why are there various different QuantiFast Probe Assays for my gene (previous versions, transcript variants)?
FAQ ID -2366
Why are the QuantiFast Probe Assays located on a single exon, thereby also detecting genomic DNA?
FAQ ID -2365
What are the main differences between the qBiomarker PCR Arrays and the RT2 Profiler PCR Arrays?
FAQ ID -2438
What are the recommended storage conditions of the QuantiFast Probe RT-PCR Plus Kit components?
FAQ ID -2355
What are the differences between the existing QuantiFast Probe RT-PCR Kit and the new Plus Kit?
FAQ ID -2358
What is the concentration of the primers and the probe in a reaction using the QuantiFast Probe Assays?
FAQ ID -2370
What is the average length of a QuantiFast Probe Assay?
FAQ ID -2374
Is the RNeasy Plus Universal Mini Kit suited for adipose, brain, and other fatty tissues?
FAQ ID -2343
Is there a size limit to the protein I can crystallize with the NexTal Cubic Phase products?
FAQ ID -2331
Is the T790M mutation included in the EGFR PCR kit?
FAQ ID -2314
Is it necessary to perform calibration steps for the use of the MAX dye in duplex RT-PCR experiments on different cyclers?
FAQ ID -2373
Is there also a miRNeasy Plus Universal Kit available?
FAQ ID -2346
I use cultured cells as starting material. Can I also use the RNeasy Plus Universal Kit?
FAQ ID -2342
I would like to order the QuantiFast Probe Assays without master mix. Is this possible?
FAQ ID -2360
My ADAPT report states "Run Invalid" and does not display any sample results. Why is this?
FAQ ID -2306
Is V600E the only mutation that can be detected with the codon 600 assay in the BRAF Pyro Kit?
FAQ ID -2382
Is the yield and quality of RNA and DNA purified with the AllPrep DNA/RNA FFPE Kit comparable to yield and quality of nucleic acids purified separately with other methods.
FAQ ID -2349
Which purification kits are recommended for the Pyro kits?
FAQ ID -2380
Which kit, the QIAprep 96 Plus Miniprep Kit or the QIAGEN Plasmid Plus 96 Miniprep Kit, do you recommend for transfection?
FAQ ID -2320
Which probe labels are available for the QuantiFast Probe Assays?
FAQ ID -2361
What should be the concentration of my protein with the NexTal Cubic Phase products?
FAQ ID -2325
What is the maximum plasmid size which I can use with the QIAprep 96 Plus Miniprep Kit or the QIAGEN Plasmid Plus 96 Miniprep Kit?
FAQ ID -2316
What plug-ins are available for the Pyrosequencing kits?
FAQ ID -2381
Which crystallization solutions are compatible with lipidic cubic phase crystallization?
FAQ ID -2418
Which deparaffinization method can I use with the AllPrep DNA/RNA FFPE Kit?
FAQ ID -2352
What species are QuantiTect Probe Assays offered for?
FAQ ID -2367
Can the insertions in Exon 20 be detected with the EGFR Pyro Kit?
FAQ ID -2383
Is it also possible to isolate miRNA with the AllPrep DNA/RNA FFPE Kit?
FAQ ID -2348
Can I use the RNeasy Plus Universal Mini Kit for all types of tissue?
FAQ ID -2338
Can the QIAprep 96 Plus Miniprep Kit or the QIAGEN Plasmid Plus 96 Miniprep Kit be used for low-copy number plasmids?
FAQ ID -2317
Can the QuantiFast Probe Assay detect short fragmented RNA as well as longer fragments of RNA?
FAQ ID -2364
Can negative crossing threshold (CT) values be negative when using the KRAS and EGFR PCR kits?
FAQ ID -2300
Can QuantiFast Probe Assays be ordered offline via e-mail or fax?
FAQ ID -2368
For the the QIAprep 96 Plus Miniprep Kit or the QIAGEN Plasmid Plus 96 Miniprep Kit, can plates be partially used?
FAQ ID -2319
For which applications can the RNA isolated with the RNeasy Plus Universal Kit also be used?
FAQ ID -2339
Does the KRAS PCR kit cover the codon 61 mutation?
FAQ ID -2308
Are the QIAprep 96 Plus Miniprep Kit or the QIAGEN Plasmid Plus 96 Miniprep Kit suited for short hairpin vectors?
FAQ ID -2318
Are the QuantiFast Probe Assays hydrolysis or FRET probes?
FAQ ID -2363
Are the buffers in the RNeasy QIAcube kit delivered in QIAcube buffer bottles?
FAQ ID -2334
Are QIAshredder spin columns delivered with the RNeasy Mini QIAcube Kit?
FAQ ID -2333
Can I do singleplex PCR with the QuantiFast Probe Assays?
FAQ ID -2362
Can I use more than 10ng DNA for the PCR reaction for the Pyro kits?
FAQ ID -2379
Are the QuantiFast Probe Assays wet-lab validated?
FAQ ID -2375
At what temperature can I perform the experiment with the NexTal Cubic Phase kits?
FAQ ID -2323
How much can the mixed standard ?Ct values vary in the KRAS PCR kit?
FAQ ID -2307
How many samples can be processed per RNeasy Mini QIAcube kit?
FAQ ID -2332
How much DNA/RNA can be isolated with the AllPrep DNA/RNA FFPE Kit?
FAQ ID -2353
How many freeze-thaw cycles can the master mix contained in the QuantiFast Probe RT-PCR Plus Kit tolerate?
FAQ ID -2357
How pure should the chloroform used with the RNeasy Plus Universal Mini Kit be?
FAQ ID -2344
How much sample material can I use for DNA/RNA purification with the AllPrep DNA/RNA FFPE Kit?
FAQ ID -2351
How much time can be saved when using the RNeasy Mini QIAcube Kit?
FAQ ID -2335
How does the QuantiFast Probe RT-PCR Plus Kit eliminate genomic DNA contamination?
FAQ ID -2354
How do I analyze the data for the particular iPSC qBiomarker Arrays I have ordered?
FAQ ID -2440
How are the genes in each iPSC qBiomarker PCR Array selected?
FAQ ID -2439
How can I adjust the ROX concentration in the QuantiFast Probe RT-PCR Plus master mix?
FAQ ID -2356
How do I harvest the crystals from meso phase? Are special tools needed with the NexTal Cubic Phase products?
FAQ ID -2330
What is the advantage of EpiTect MethyLight PCR Kits over QuantiTect Probe PCR Kits for methylation studies?
FAQ ID -2010
What is the advantage of using the Type-it Microsatellite PCR Kit?
FAQ ID -2059
What is a good starting amount of template DNA for PCR with the EpiTect MSP Kit?
FAQ ID -2005
What is the advantage of using the Type-it Mutation Detect PCR Kit?
FAQ ID -2064
What is the maximum lifetime of the QIAGEN rotor for the Centrifuge 4 and 4K?
-16
What is the principle behind the DNA Polymerase in the EpiTect MSP Kit?
FAQ ID -2003
What is the maximum DNA yield from forensic samples processed with the QIAsymphony SP?
FAQ ID -2028
Is it possible to use different sample types in one run with the MagAttract 96 cador Pathogen Kit?
FAQ ID -2072
Is magnetic particle carry-over in the eluates on the BioSprint 96 normal?
FAQ ID -2073
What happens when using EpiTect MethyLight Assays on only partially bisulfite converted DNA template?
FAQ ID -2009
What are the expected PCR results when using EpiTect Control DNA on untreated or bisulfite-converted DNA?
FAQ ID -2011
What are the features of the expression vector used for QIAgenes Insect/Mammalia?
FAQ ID -2050
Why is CoralLoad included in the Type-it Mutation Detect, but not in the Type-it Microsatellite PCR Kit?
FAQ ID -2068
Why do casework-sample protocols for the QIAsymphony DNA Investigator Kit start with different lysate volumes (200 µl, 500 µl, or 1000 µl)?
FAQ ID -2035
Why do pQE-T7 vectors of the QIAgenes E. coli system have an additional UAG amber stop codon?
FAQ ID -2043
Why is the Type-it Mutation Detect PCR Kit recommended for preamplification of SNPs?
FAQ ID -2067
Why is maximum amplicon size for the Type-it Microsatellite PCR Kit limited to 500 bp?
FAQ ID -2060
Why is special Type-it Fast SNP Probe PCR chemistry required for TaqMan® SNP Genotyping?
FAQ ID -2057
Will the optimized DNA sequence for QIAgenes be provided?
FAQ ID -2045
What sample types can be processed using the QIAsymphony Virus/Bacteria protocols?
FAQ ID -2036
What sample volumes can be processed with the QIAsymphony Virus/Bacteria Kits?
FAQ ID -2037
Can data be analyzed on the Rotor-Gene Q while a run is in progress?
FAQ ID -2091
Are there recommendations for sensitive detection of underrepresented fragments using the Type-it Mutation Detect PCR Kit?
FAQ ID -2066
Are there two vectors for protein expression using QIAgenes in insect or mammalian cells?
FAQ ID -2048
Is mitochondrial DNA isolated with the QIAsymphony DNA Investigator Kit on the QIAsymphony SP?
FAQ ID -2030
Is it possible to edit the sample sheet in the Rotor-Gene Q software after a PCR run?
FAQ ID -2092
Is it possible to import a standard curve from a previous PCR run on the Rotor-Gene Q?
FAQ ID -2093
What kind of samples should be processed using the large-volume protocol with the QIAsymphony DNA Investigator Kit?
FAQ ID -2034
Which QIAsymphony Virus/Bacteria Kit size should be used for each protocol?
FAQ ID -2038
Which expression systems can be used with the QIAgenes Insect/Mammalia expression constructs?
FAQ ID -2049
What types of reaction vessels are required for use in the Rotor-Gene Q?
FAQ ID -2085
Which capillary sequencers can be used for detection of amplicons from the Type-it Microsatellite PCR Kits?
FAQ ID -2063
Can the EpiTect PCR Control DNAs be used for mouse and rat assays?
FAQ ID -2008
Can the EpiTect PCR Control DNAs also be used as standard in real-time methylation specific quantification?
FAQ ID -2006
Can the protocols for purification of small RNAs from cells in the appendices of the AllPrep DNA/RNA 96 Handbook also be used with tissue samples?
FAQ ID -2001
Can QIAGEN rotors used with the centrifuges 4C and 4KC also be used with 4-16 and 4-16K models?
-15
Can the MagAttract 96 cador Pathogen Kit also be used on the KingFisher 96 or MagMAX Express 96 instruments?
FAQ ID -2074
Can TopTaq Master Mix simply be stored at room temperature on the lab bench for up to 4 months?
FAQ ID -2052
Do all QIAsymphony DNA Investigator protocols use carrier RNA?
FAQ ID -2033
Can the Type-it Microsaltellite PCR Kit be used on cyclers with fast ramping rates?
FAQ ID -2062
Can the Type-it Mutation Detect PCR kit be used for multiplex PCR of fragments longer than 500 bp?
FAQ ID -2065
Can 0.1 ml and 0.2 ml tubes used on the Rotor-Gene Q be labeled?
FAQ ID -2082
Can DNase digestion be performed on the RNeasy 96 plate during the RNA purification part of the Allprep DNA/RNA 96 procedure?
FAQ ID -2000
How many signal readings are taken during the real-time data acquisition on the Rotor-Gene Q?
FAQ ID -2083
Can membrane proteins be expressed with QIAgenes expression kits?
FAQ ID -2047
Do QIAsymphony Virus/Bacteria samples require pretreatment before they are loaded onto the instrument?
FAQ ID -2039
Does the centrifugal force have any effect on the kinetics of a PCR reaction on the Rotor-Gene Q?
FAQ ID -2081
Does an internal control have to be included for each QIAsymphony Virus/Bacteria protocol run?
FAQ ID -2040
How can full-length genes or individual domains of the QIAgenes Insect/Mammalia constructs be subcloned?
FAQ ID -2051
Can frozen samples be used with the MagAttract 96 cador Pathogen Kit?
FAQ ID -2070
How fast does the rotor spin during a run on the Rotor-Gene Q?
FAQ ID -2080
How is the log graph threshold value (Ct) for the Rotor-Gene Q determined?
FAQ ID -2088
How long can DNA converted with EpiTect Bisulfite Kits be stored before use in methylation specific PCR with the EpiTect MSP Kit?
FAQ ID -2002
How will running less than 96 samples with the MagAttract 96 cador Pathogen kit affect usage of plasticware?
FAQ ID -2071
If new dyes or chemistries appear on the real-time PCR market, will Rotor-Gene Q be capable of running those dyes?
FAQ ID -2087
If orders cannot be placed online, how can QIAgenes be ordered?
FAQ ID -2046
How much space does the PyroMark Q24 instrument need?
FAQ ID -2098
Does the Type-it Microsatellite PCR Kit always require an annealing temperature of 60°C, or can pre-optimized annealing temperatures for target-primer systems be used?
FAQ ID -2061
What is the turn around time for the ordering of miScript Target Protector?
FAQ ID -2254
What is the optimal range of PCR fragment sizes for normalization with the SeqTarget System?
FAQ ID -2247
What is the processing time for 96 normalizations with the SeqTarget System?
FAQ ID -2237
What technologies are used by the KRAS and EGFR PCR kits?
FAQ ID -2286
What method do you recommend to quantify DNA before running the KRAS and EGFR PCR kit?
FAQ ID -2292
What kind of automation can be done with the EasyXtal 15-well Tool?
FAQ ID -2220
What format are the EasyXtal 15-well tools?
FAQ ID -2219
What dyes are used in the KRAS and EGFR PCR kits?
FAQ ID -2288
What do I do if I get amplification in my no template control (NTC) with the KRAS and EGFR PCR kits?
FAQ ID -2298
What do I need to provide when ordering a miScript Target Protector?
FAQ ID -2251
What is the difference of the sequence of a miScript miRNA Inhibitor and miScript Target Protector?
FAQ ID -2253
What is a miScript Target Protector?
FAQ ID -2249
What is a miScript Precursor Assay?
FAQ ID -2214
Which transfection reagent is recommended for transfection of the miScript Target Protectors?
FAQ ID -2252
Which variants of the EasyXtal 15-well tool are available?
FAQ ID -2221
Why are most of my samples outside of the cluster in supervised mode using Rotor-Gene ScreenClust HRM Software?
FAQ ID -2205
Why are some of my samples outside of the cluster using Rotor-Gene ScreenClust HRM Software?
FAQ ID -2204
Why does the miScript Target Protector not induce the degradation process?
FAQ ID -2263
Why does the SeqTarget Prep Protocol require a 2-step procedure?
FAQ ID -2238
What will happen, if the normalized DNA for the SeqTarget System is eluted with Buffer EB instead of EB2?
FAQ ID -2242
When negative results are generated using the KRAS and EGFR PCR kits, do I rerun them?
FAQ ID -2294
What type of samples can I use for the KRAS PCR and EGFR kits?
FAQ ID -2293
What voltage should be used to run my agarose gel with the GelPilot Agarose?
FAQ ID -2261
Which mode should I use in the Rotor-Gene ScreenClust HRM Software, supervised or unsupervised?
FAQ ID -2202
Do the KRAS and EGFR PCR kits contain a passive reference dye?
FAQ ID -2295
Does the atypical look of the lyophilized FlexiTube siRNA Premix have a negative impact on performance?
FAQ ID -2267
Does the miScript Target Protector match the target sequence?
FAQ ID -2250
How do I store my FlexiTube siRNA Premix?
FAQ ID -2270
Do I have to order at least 4 FlexiTube siRNA Premixes?
FAQ ID -2266
How is ARMS technology superior to alternative methods such as dye terminator sequencing?
FAQ ID -2290
How do I search for all the miScript Target Protectors for one species?
FAQ ID -2255
How can I check the success of the normalization procedure for the SeqTarget System?
FAQ ID -2245
Can PCR fragments be normalized for the SeqTarget System after purification with QIAquick?
FAQ ID -2240
Are all 3 components of the SeqTarget System required or can a different Long Range PCR Kit and primers be used?
FAQ ID -2234
Can PCR fragments be normalized for the SeqTarget System without a previous purification step?
FAQ ID -2246
Can I use a 384 well plate for the KRAS and EGFR PCR kits?
FAQ ID -2296
Can the miScript Precursor Assay differentiate between pri- and pre-miRNA?
FAQ ID -2211
Does ethanol from the PCR product purification influence the normalization procedure for the SeqTarget System?
FAQ ID -2244
What are EasyXtal 15-well Tools used for?
FAQ ID -2217
What are Principal Components analyzed in Rotor-Gene ScreenClust HRM Software?
FAQ ID -2200
What are the amplicon lengths of the KRAS and EGFR PCR kits?
FAQ ID -2289
What are clusters analyzed in the Rotor-Gene ScreenClust HRM Software?
FAQ ID -2201
Is the SeqTarget System compatible with standard-size PCRs?
FAQ ID -2241
What controls are used in the KRAS and EGFR PCR kits to ensure reliable results?
FAQ ID -2291
What does QIAGEN use for a positive control in precursor detection experiments?
FAQ ID -2212
What do I do if I cannot see amplification in the FAM channel in my KRAS and EGFR PCR kits?
FAQ ID -2297
What are the corresponding QIAGEN names for former Biotage instruments?
FAQ ID -2285
What can be the reason for low DNA yields after normalization with the SeqTarget System?
FAQ ID -2239
How much buffer should be used for agarose gel electrophoresis?
FAQ ID -2257
How long does it take to generate results using the KRAS and EGFR PCR kits?
FAQ ID -2299
How should gels be cast with the GelPilot Agarose so that optimal resolution is achieved?
FAQ ID -2258
How long should PCR fragments be for the SeqTarget System?
FAQ ID -2236
Is it possible to use lower siRNA concentrations with FlexiTube siRNA Premix?
FAQ ID -2264
How many miScript Precursor Assays do you offer?
FAQ ID -2213
Is one able to use FlexiTube siRNA Premix for transfection of primary adherent cell types?
FAQ ID -2268
If I am working with a difficult-to-transfect cell type or if I obtain only weak silencing effects what can I do?
FAQ ID -2265
Is one able to use FlexiTube siRNA Premix for transfection of primary suspension cell types?
FAQ ID -2269
Is it possible to normalize to more or less than 500 ng with the SeqTarget System if needed?
FAQ ID -2235
How many samples can be processed with the QIAsymphony DNA Mini Kit and the Virus Blood 200 protocol?
FAQ ID -2159
How often can the Strep-Tactin Superflow Plus resin be reused?
FAQ ID -2169
Is it possible to purify and normalize long range PCRs produced with PCR kits from other vendors for the SeqTarget System?
FAQ ID -2243
If housekeeping and target genes differ significantly in abundance, will they both be amplified with equal efficiency using the Rotor-Gene Multiplex PCR Kit?
FAQ ID -2129
If Vapor-Lock is used to overlay PCR reactions, which volume should be entered as reaction volume in the Rotor-Gene Q Software?
FAQ ID -2150
Is it possible to purify a GST-tagged protein under denaturing conditions using Glutathione Superflow?
FAQ ID -2172
Is it normal that the binding capacity of GST-resins decreases with flow rate?
FAQ ID -2174
Why is the initial activation step different for Rotor-Gene Probe, SYBR Green and Multiplex Kits?
FAQ ID -2118
Will Rotor-Gene Kits also work on the Rotor-Gene 6000 and 3000 cyclers?
FAQ ID -2121
Why is there an asterisk for my miScript Primer Assays?
FAQ ID -2176
Will Uracil-N-Glycosylase (UNG) completely remove contaminating amplicons when using QuantiTect +UNG Kits?
FAQ ID -2139
Will QuantiTect Primer Assays work with Rotor-Gene SYBR Green Kits using an annealing step at 60ºC?
FAQ ID -2124
What is miRNA*? Do I need to consider this in my profiling experiment?
FAQ ID -2179
What are the main differences between Rotor-Gene and QuantiTect or QuantiFast PCR Kits?
FAQ ID -2119
What are possible reasons for reduced DNA yields with REPLI-g Kits?
FAQ ID -2148
What is the residuals plot in the Rotor-Gene ScreenClust HRM Software?
FAQ ID -2199
What is the target nucleic acid for the Virus Blood 200 Protocol on the QIAsymphony?
FAQ ID -2158
What is the detection limit of the Rotor-Gene and QuantiFast Multiplex RT-PCR Kits?
FAQ ID -2144
What is the minimum number of assays that can be ordered on QuantiTect Primer Assay Plates?
FAQ ID -2109
What is the maximum number of targets that can be amplified per reaction with the QuantiFast Multiplex RT-PCR Kit?
FAQ ID -2145
How should DNA isolated on the QIAsymphony be stored?
FAQ ID -2165
How should samples be stored before purification using the Virus Blood 200 Protocol on the QIAsymphony?
FAQ ID -2157
How pure should the chloroform used with the RNeasy Microarray Tissue Mini Kit be?
FAQ ID -2193
Why should I use the RNeasy Microarray Tissue Mini Kit when purifying RNA for microarray analysis?
FAQ ID -2188
Which elution volumes can be selected using the QIAsymphony Virus Blood 200 protocol?
FAQ ID -2160
What sample volumes can be used with the Virus Blood 200 Protocol on the QIAsymphony?
FAQ ID -2156
Which is the best condition for eluting GST-tagged proteins from Glutathione Superflow?
FAQ ID -2173
What species are QuantiTect Primer Assay Plates offered for?
FAQ ID -2110
Which plate formats are offered for QuantiTect Primer Assay Plates?
FAQ ID -2106
Why are the denaturation and annealing/extension times for QuantiFast Multiplex RT-PCR Kits much shorter than those for QuantiFast Multiplex PCR Kits?
FAQ ID -2147
Which sample types can be processed using the QIAsymphony Virus Blood 200 protocol?
FAQ ID -2154
How many reactions can be performed per well of the QuantiTect Primer Assay Plates?
FAQ ID -2107
Can assays for 20 and 100 reactions be ordered on the same QuantiTect Primer Assay Plate?
FAQ ID -2108
Can QuantiTect Primer Assay Plates be ordered offline via e-mail or fax?
FAQ ID -2112
Can Glutathione Superflow resin be reused?
FAQ ID -2171
Can Uracil-N-Glycosylase (UNG) be added to the QuantiTect PCR Master Mix and be stored for later use?
FAQ ID -2138
Can Uracil-N-Glycosylase (UNG) be purchased separately, independent of QuantiTect PCR Kits?
FAQ ID -2134
Are there any changes in the procedure using the Strep-Tactin Superflow Plus resin with the higher capacity over the standard matrix?
FAQ ID -2168
Can Uracil-N-Glycosylase (UNG) be used with a one-step RT-PCR kit, such as the QuantiTect Probe RT-PCR Kit?
FAQ ID -2135
Can the Rotor-Gene Multiplex RT-PCR Kit be used on other cyclers?
FAQ ID -2142
Can RNA isolated with the RNeasy Microarray Tissue Mini Kit also be used in downstream applications other than microarray analysis?
FAQ ID -2191
Are QuantiTect PCR +UNG Kits available in bulk format (e.g., 25 ml master mix)?
FAQ ID -2136
Are Rotor-Gene Kits compatible with reaction setup using the QIAgility instrument?
FAQ ID -2116
How does template quality influence the results with the Type-it HRM PCR Kit?
FAQ ID -2197
Are the layouts for both 96- and 384-well QuantiTect Primer Assay Plates visible when ordering online?
FAQ ID -2111
Do you have protocols for software setup on various PCR platforms using QuantiFast Probe RT-PCR Kits?
FAQ ID -2105
How does magnetic-particle carry-over in QIAsymphony eluates need to be handled?
FAQ ID -2166
How does the RNeasy Microarray Tissue Mini Kit prevent traces of phenol from negatively affecting the reverse-transcription step in microarray experiments?
FAQ ID -2189
How do Rotor-Gene Probe Kits compare with QuantiFast Probe Kits?
FAQ ID -2125
How many elution steps are recommended when using Glutathione Superflow resin?
FAQ ID -2175
Do you have a protocol for Rotor-Gene software setup for the Rotor-Gene SYBR Green PCR and RT-PCR Kits?
FAQ ID -2104
Does an internal control have to be used with the QIAsymphony Virus Blood 200 Protocol?
FAQ ID -2162
Can Vapor-Lock be used with samples processed on the QIAgility?
FAQ ID -2153
Do the master mixes in Rotor-Gene Kits contain dUTP to allow UNG pretreatment?
FAQ ID -2117
Do QuantiTect Primer Assays also work with Rotor-Gene SYBR Green PCR Kits?
FAQ ID -2123
Do limiting primer concentrations need to be determined when using the Rotor-Gene Multiplex PCR Kit?
FAQ ID -2128
What buffer should be used to dilute cDNA made using the miScript Reverse Transcription Kit?
FAQ ID -1601
Is FlexiTube GeneSolution available for other scales than the 1 nmol scale?
FAQ ID -1666
Is it necessary to clean up cDNA prepared with the QuantiTect Whole Transcriptome Kit?
FAQ ID -1610
Is the master mix of QuantiFast Kits for real-time PCR aliquoted into several tubes to prevent cross-contamination?
FAQ ID -1697
Is the QuantiTect Whole Transcriptome Kit suitable for miRNA amplification?
FAQ ID -1609
Is it necessary to include a genomic DNA removal step with the QuantiTect Whole Transcriptome Kit?
FAQ ID -1614
Is sequence information for HP Validated siRNAs provided in the FlexiTube format?
FAQ ID -1665
Where can I find background information and literature on Whole Genome Amplification with REPLI-g Kits?
FAQ ID -1690
Why is there DNA in the no-template (negative) control when using the QuantiTect Whole Transcriptome Kit?
FAQ ID -1620
What is the maximum volume of RNA in solution that can be used with the QuantiTect Whole Transcriptome Kit?
FAQ ID -1616
Can the Ni-NTA and Strep-Tactin Superflow Cartridges be reused?
FAQ ID -1607
Can the Ni-NTA Superflow Cartridges and/or Strep-Tactin Cartridges be connected in series?
FAQ ID -1606
Can the QuantiTect Whole Transcriptome Kit be used for amplification of RNA from LCM samples?
FAQ ID -1612
Can I store cDNA generated by the miScript System?
FAQ ID -1602
Does the QuantiTect Whole Transcriptome Kit work with RNA purified from bacteria, yeast, or plants?
FAQ ID -1615
Can T-Script® enzyme of the QuantiTect Whole Transcriptome Kit be substituted by Quantiscript Reverse Transcriptase?
FAQ ID -1617
Can the QuantiTect Whole Transcriptome reaction be stopped after the reverse transcription step to proceed with the ligation and amplification steps at a later time?
FAQ ID -1618
Can I still reorder siRNA that has been removed from the GeneGlobe data base?
FAQ ID -1668
Can amplification with the QuantiTect Whole Transcriptome Kit be extended to more than 8 hours to improve cDNA yields?
FAQ ID -1608
Can I select HP Validated and HP GenomeWide siRNAs for FlexiTube?
FAQ ID -1658
Can cDNA prepared with the QuantiTect Whole Transcriptome Kit be reampliied using REPLI-g Kits?
FAQ ID -1611
Can FlexiTube siRNA or FlexiTube GeneSolution products be ordered in solution?
FAQ ID -1667
How fast is the 6xHis-tagged protein purification process using Ni-NTA Superflow Cartridges?
FAQ ID -1605
How long is cDNA generated with the QuantiTect Whole Transcriptome Kit?
FAQ ID -1613
How long can I archive cDNA generated with the QuantiTect Whole Transcriptome Kit?
FAQ ID -1623
Do I need to use the miScript Universal Primer for detection of mRNA?
FAQ ID -1600
Have you observed co-amplification of genomic DNA from RNA templates used in the QuantiTect Whole Transcriptome Procedure?
FAQ ID -1619
Do you offer a QuantiFast Kit for one-step RT-PCR?
FAQ ID -1695
Do you have a protocol for purification of total RNA from plant latex?
FAQ ID -1694
Do you have a protocol for purification of total RNA from plant tissue with the RNeasy Lipid Tissue Mini Kit?
FAQ ID -1693
What effect does homogenization have on DNA yield and integrity when using AllPrep DNA/RNA Kits?
FAQ ID -1751
Is TopTaq DNA Polymerase a hot-start polymerase?
FAQ ID -1748
What is the fidelity of TopTaq DNA Polymerase?
FAQ ID -1739
Which nucleotide analogs can be used with TopTaq DNA Polymerase?
FAQ ID -1741
Why might Affymetrix GeneChip Mapping assays interfere with the REPLI-g FFPE kit?
FAQ ID -1755
What is the shelf-life of TopTaq DNA Polymerase and Master Mix?
FAQ ID -1743
What is the principle of the REPLI-g FFPE Kit?
FAQ ID -1753
What is the largest plasmid size that can be purified using the QuickLyse Miniprep Kit?
FAQ ID -1752
What is the minimum amount of starting material that can be used with the RNeasy Plus Micro and AllPrep DNA/RNA Micro Kit?
FAQ ID -1750
What is the recommended sample size for use with the REPLI-g FFPE Kit?
FAQ ID -1756
Can small miRNA-containing RNA fractions be separated from large RNAs using the miRNeasy FFPE Kit?
FAQ ID -1737
Can miRNA and other RNAs smaller than 200 nucleotides be isolated with the AllPrep DNA/RNA Micro Kit or RNeasy Plus Micro Kit?
FAQ ID -1749
Can TA/UA cloning be done with PCR products amplified with TopTaq DNA Polmyerase and TopTaq Master Mix Kits?
FAQ ID -1740
Are there problems with the default threshold setting on the Applied Biosystems 7500 when using QuantiFast Probe PCR Kits?
FAQ ID -1702
At which step in the REPLI-g FFPE protocol should PicoGreen quantification be done?
FAQ ID -1758
How can foaming of Buffer RLT Plus lysates be avoided when using RNeasy Plus Micro and Mini Kits, or the Allprep DNA/RNA Mini Kit?
FAQ ID -1734
Do you have protocols for sample processing on the Autopure LS instrument?
FAQ ID -1759
Have QuantiTect Primer Assays been tested with QuantiFast SYBR Green PCR Kits on the Mastercycler ep realplex?
FAQ ID -1714
Can the REPLI-g FFPE procedure restore DNA derived from FFPE tissue samples in the original order?
FAQ ID -1754
Can the REPLI-g FFPE Kit also be used for formalin-fixed, ethanol preserved samples?
FAQ ID -1760
Do you have a protocol for the isolation of total RNA from paraffin-embedded sections on the Biorobot M48?
FAQ ID -1733
Does TopTaq Stabilizer of the TopTaq DNA Polymerase and Master Mix Kits interfere with downstream applications?
FAQ ID -1744
Do you have a forensic post-PCR purification protocol to purify double-stranded DNA fragments from PCR reactions?
FAQ ID -1761
Is sample pretreatment required for purifying RNA on the QIAsymphony SP?
FAQ ID -1938
Is the QIAsymphony RNA Kit compatible with stabilized samples?
FAQ ID -1944
Is RNA co-purified with DNA using QIAsymphony DNA Tissue protocols?
FAQ ID -1920
Is there a charge for user training on the QIAsymphony SP System?
FAQ ID -1900
What are the expected DNA yields from tissue processed on the QIAsymphony SP?
FAQ ID -1919
QuantiTect Primer Assays are bioinformatically validated, genomewide primer sets. What does “bioinformatically validated” mean?
FAQ ID -1982
What is the range of elution volumes on the QIAsymphony SP?
FAQ ID -1908
How many samples can be processed per kit or per reagent cartridge for RNA purification on the QIAsymphony SP?
FAQ ID -1940
How does QIAsymphony SP keep cellular samples in suspension prior to pipetting so that samples do not settle?
FAQ ID -1905
How many PCR reactions can be performed for different scales of miScript Primer Assays?
FAQ ID -1990
How efficient is DNA removal by a gDNA Eliminator 96 plate compared with DNase digestion on an RNeasy 96 plate?
FAQ ID -1994
How long can homogenates remain on the QIAsymphony SP without risk of RNA degradation?
FAQ ID -1941
How much sample can be used with the Allprep DNA/RNA 96 Kit?
FAQ ID -1997
Which types and amounts of sample material can be processed for RNA extraction on the QIAsymphony SP?
FAQ ID -1937
How much starting material can be used with the QIAsymphony DNA Mini Kit on the QIAsymphony SP?
FAQ ID -1917
What sample types can be used with the AllPrep DNA/RNA 96 Kit?
FAQ ID -1996
What sample types should be processed with the High Content (HC) protocol for DNA extraction on the QIAsymphony SP?
FAQ ID -1918
What sample types can be used with the RNeasy Plus 96 Kit?
FAQ ID -1991
Will mitochondrial DNA also be isolated using the DNA Tissue protocols on the QIAsymphony SP?
FAQ ID -1921
Why does the Allprep DNA/RNA 96 Kit use Buffer RLT, whereas the AllPrep DNA/RNA Mini Kit uses Buffer RLT Plus?
FAQ ID -1999
Why is there no vacuum-only protocol for the RNeasy Plus 96 Kit?
FAQ ID -1993
What is the turn around time for custom miScript miRNA mimics, inhibitors and primer assays?
FAQ ID -1985
What kind of molecules are miScript miRNA Inhibitors?
FAQ ID -1983
Is it possible to modify QIAsymphony protocols?
FAQ ID -1903
What are the expected yields of total RNA isolated with the QIAsymphony SP?
FAQ ID -1942
What are the starting volumes of homogenate for RNA extraction on the QIAsymphony SP?
FAQ ID -1939
What sample input volumes can be used on the QIAsymphony SP?
FAQ ID -1907
What sample types can be processed using the tissue protocols for DNA extraction on the QIAsymphony SP?
FAQ ID -1915
What kind of molecules are miScript miRNA Mimics?
FAQ ID -1986
What RNA elution volumes are available with the QIAsymphony RNA Kit?
FAQ ID -1943
Do limiting primer concentrations need to be determined when using QuantiFast Multiplex PCR Kits?
FAQ ID -1976
Do samples require pretreatment before loading onto the QIAsymphony SP for DNA extraction?
FAQ ID -1916
Can uneven sample numbers be processed on the QIAsymphony SP module?
FAQ ID -1906
Does HiPerFect work for the transfection of miScript miRNA mimics and inhibitors?
FAQ ID -1984
Does the QIAsymphony SP system generate run reports and/or log files?
FAQ ID -1911
Do you have a protocol for the purification of archive-quality DNA from 100 to 5 x 10e8 cultured cells using Gentra Puregene Kits?
FAQ ID -1960
Do you have a protocol for the purification of archive-quality DNA from 0.5–20 mg paraffin-embedded tissue using Gentra Puregene Kits?
FAQ ID -1964
回到顶部