常见问题

 

您无权限查看此资源

Can the QIAcube heater/shaker be used independently from protocol runs?
FAQ ID -1422
Can the QIAcube Connect rotor and/or buckets be removed for cleaning?
FAQ ID - 141491
Can the QIAcube Connect MDx heater/shaker be used independently from protocol runs?
148741
Can the QIAcube Connect MDx centrifuge be used independently from protocol runs?
148742
Can the QIAcube Connect heater/shaker be used independently from protocol runs?
FAQ ID - 141502
Can the QIAcube centrifuge be used independently from protocol runs?
FAQ ID -1423
Can the QIAcube be connected to a laboratory information management system?
148732
Can the QIAcube be connected to a laboratory information management system?
FAQ ID -1411
Can the QIAamp MinElute Virus Kits be used to extract viral nucleic acid from cell culture supernatant?
FAQ ID -472
Can the PyroMark Q96 CpG LINE assay be used with an ID system?
2861
Can the protocols for purification of small RNAs from cells in the appendices of the RNeasy Plus 96 Handbook also be used with tissue samples?
FAQ ID -1995
Can the protocols for purification of small RNAs from cells in the appendices of the AllPrep DNA/RNA 96 Handbook also be used with tissue samples?
FAQ ID -2001
Can the PAXgene RNA stabilization solution/bone marrow mixture from the PAXgene Bone Marrow RNA Tube be disposed of by adding bleach?
FAQ ID -2515
Can the PAXgene RNA stabilization solution/blood mixture from the PAXgene Blood RNA Tube be disposed of by adding bleach?
FAQ ID - 3469
Can the PAXgene Bone Marrow RNA System be used with animal bone marrow or with blood samples?
FAQ ID -2501
Can the PAXgene Bone Marrow RNA System be used to isolate genomic DNA or viral RNA and DNA?
FAQ ID -2507
Can the PAXgene Bone Marrow RNA System be used for other human body fluids than bone marrow samples or cultured cells of human origin?
FAQ ID -2502
Can the PAXgene Blood RNA Tubes undergo freeze/thaw cycles?
FAQ ID -2471
Can the PAXgene Blood RNA System be used with animal blood samples?
FAQ ID - 3461
Can the PAXgene Blood RNA System be used to isolate viral RNA?
FAQ ID - 3465
Can the PAXgene Blood RNA System be used to isolate DNA?
FAQ ID - 3466
Can the PAXgene Blood RNA MDx Kit be used with both the BioRobot Universal System and the BioRobot MDx?
FAQ ID -2489
Can the PAXgene Blood RNA MDx Kit be used for diagnostic or prognostic procedures?
FAQ ID -2488
Can the PAXgene Blood miRNA System be used for diagnostic or prognostic procedures?
FAQ ID -2494
Can the PAXgene Blood DNA Tubes be stored at higher temperatures?
FAQ ID - 3494
Can the PAXgene Blood DNA Tubes be stored at 4°C?
FAQ ID - 3493
Can the PAXgene Blood DNA Tube be frozen? If yes, at what temperatures and for how long?
FAQ ID - 3491
Can the PAXgene 96 Blood RNA Kit be used for diagnostic or prognostic procedures?
FAQ ID - 3471
Can the nuclei and cell debris fraction generated in the QProteome Mitochondria isolation procedure be used for further fractionation?
FAQ ID -1185
Can the Ni-NTA Superflow Cartridges and/or Strep-Tactin Cartridges be connected in series?
FAQ ID -1606
Can the Ni-NTA and Strep-Tactin Superflow Cartridges be reused?
FAQ ID -1607
Can the new CRISPR PCR Assays be used for dPCR on the QIAcuity? Do you have data or a protocol?
3795
Can the miScript Single Cell qPCR Kit be used with plant cells?
FAQ ID - 3568
Can the miScript Single Cell qPCR Kit be used to quantify piRNA expression?
FAQ ID - 3567
Can the miScript Precursor Assay differentiate between pri- and pre-miRNA?
FAQ ID -2211
Can the miScript Plant RT Kit be used to quantify piRNA expression?

FAQ ID - 3434

Can the miScript Plant RT Kit be used to quantify animal miRNAs?
FAQ ID - 3433
Can the miScript II RT Kit be used to quantify plant miRNAs?
FAQ ID - 3431
Can the miScript II RT Kit be used to quantify piRNAs?
FAQ ID - 3432
Can the MaXtract High Density Tube be used with QIAzol to isolate RNA?
FAQ ID -1304
Can the MAPK1 siRNA of the RNAi Human/Mouse Starter Kit be purchased separately?
FAQ ID -744
Can the MAPK1 control siRNA be used for rat cells?
FAQ ID -547
Can the MagAttract 96 cador Pathogen Kit also be used on the KingFisher 96 or MagMAX Express 96 instruments?
FAQ ID -2074
Can the lysis volume be reduced for higher sensitivity?
FAQ ID - 143763
Can the kit be used on the EZ2 Connect?
FAQ ID - 3845
Can the kit be used on the EZ1 Advanced XL?
FAQ ID - 3844
Can the kit be run on EZ1 instruments?
FAQ ID - 3852
Can the Internal Controls be ordered separately in the QuantiFast Pathogen + IC kit for use during purification?
FAQ ID -2451
Can the Internal Control DNA or RNA be added directly to the sample?
FAQ ID -2602
Can the insertions in Exon 20 be detected with the therascreen EGFR Pyro Kit?
Can the insertions in Exon 20 be detected with the EGFR Pyro Kit?
FAQ ID - 3279
Can the insertions in Exon 20 be detected with the EGFR Pyro Kit?
FAQ ID -2383
Can the FastLane RT reaction be incubated longer than 30 min at 42 deg C?
FAQ ID -3079
Can the FastLane Cell lysates (containing stabilized RNA, step 8. in Fastlane protocol) be stored?
FAQ ID -3077
Can the FastLane Cell cDNA Kit be used with tissue?
FAQ ID -821
Can the FastLane Cell cDNA Kit also be used for qualitative end-point PCR?
FAQ ID -834
Can the EZ2 AdnaTest be used for other body fluids than blood?
3833
Can the EZ1 DNA Investigator Tip Dance protocol cope with any solid matrix remaining in the sample tube?
FAQ ID -1153
Can the EZ1 DNA Forensic Card be used with the EZ1 DNA Investigator Kit?
FAQ ID -1151
Can the EpiTect PCR Control DNAs be used for mouse and rat assays?
FAQ ID -2008
Can the EpiTect PCR Control DNAs also be used as standard in real-time methylation specific quantification?
FAQ ID -2006
Can the EpiTect Methyl qPCR System technology reliably characterize heterogenous tissue samples?
FAQ ID -2732
Can the EasyXtal 15-Well Tools be reused?
FAQ ID -1117
Can the EasyXpress Linear Template Fab Kit be used to express entire IgGs?
FAQ ID -2966
Can the DyeEx 96 kit be used to clean sequencing reactions to be run on ABI 3730?
FAQ ID -3075
Can the disc and absorber strips for the Pyromark Q48 be reused?
FAQ ID - 3720
Can the computer for the LiquiChip Workstation be networked?
FAQ ID -629
Can the CGT Viral Vector Lysis kit be used with assays of other suppliers or custom designed assays?
FAQ ID - 3982

Can the CGT dPCR assays also be used for the quantification of non-AAV samples?
FAQ ID - 3868
Can the CGT dPCR assays also be used for the analysis of AAV genome integrity?
FAQ ID - 3867
Can the Certal CHO Positive Control DNA be used to create a qPCR standard for relative quantification?
FAQ ID -2979
Can the CB and RB sample tubes be used interchangeably?
FAQ ID - 141510
Can the Blocking Reagent supplied with the Penta-His HRP Conjugate be used with other antibodies?
FAQ ID -1073
Can the BioSprint instruments be placed in a cool room?
FAQ ID -938
Can the bacterial pellet in the QuickLyse Miniprep procedure be resuspended by pipetting up and down or shortening the vortexing time?
FAQ ID -1352
Can the AllPrep RNA/Protein Kit be used with tissue?
FAQ ID -1095
Can the AllPrep DNA/RNA/Protein Mini Kit be used with fibrous or lipid tissues?
FAQ ID -1579
Can the 2nd Proteinase K step be omitted?
3758
Can the 2nd Prot K step be omitted?
153896
Can TA/UA cloning be done with PCR products amplified with TopTaq DNA Polmyerase and TopTaq Master Mix Kits?
FAQ ID -1740
Can Taq DNA Polymerase use RNA as a template, and generate false positives in "no-RT" controls?
FAQ ID -523
Can T-Script® enzyme of the QuantiTect Whole Transcriptome Kit be substituted by Quantiscript Reverse Transcriptase?
FAQ ID -1617
Can specific types of white blood cells be enriched from a PAXgene Bone Marrow RNA Tube prior to the RNA isolation?
FAQ ID -2508
Can specific types of white blood cells be enriched from a PAXgene Blood RNA Tube prior to the RNA isolation?
FAQ ID - 3467
Can solid samples be processed with the QIAsymphony DNA Investigator Kit on the instrument?
FAQ ID -2027
Can smaller volumes of blood be processed from the PAXgene Blood DNA Tube?
FAQ ID - 3500
Can small miRNA-containing RNA fractions be separated from large RNAs using the miRNeasy FFPE Kit?
FAQ ID -1737
Can siRNA silence bacterial genes?
FAQ ID -395
Can single-stranded DNA be removed by gDNA Eliminator columns?
FAQ ID -3152
Can several extraction steps be performed in the same MaXtract tube?
FAQ ID -1302
Can sections of PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue be used for other histochemical staining techniques, such as PAS?
FAQ ID -2530
Can sections of PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue be used for other histochemical staining techniques, such as PAS?
FAQ ID -3041
Can samples in Allprotect Tissue Reagent undergo repeated freeze-thaws without degradation of DNA, RNA, and protein?
FAQ ID -1575
Can samples be lysed overnight with Proteinase K in the QIAamp DNA FFPE Advanced procedure?
153893
Can samples be lysed overnight with Proteinase K in the EZ1&2 DNA FFPE procedure?
3755
Can saliva in PAXgene Saliva Collector tubes be stored frozen at –20 or –80°C?
3817
Can saliva in PAXgene Saliva Collector tubes be stored at temperatures higher than 25°C?
3816
Can Rotor-Gene Reports be modified?
FAQ ID -3184
Can RNAprotect Tissue Reagent be used to store previously isolated RNA?
FAQ ID -337
Can RNAprotect Tissue Reagent be used to stabilize tissue which has already been flash-frozen?
FAQ ID -757
Can RNAprotect Saliva Reagent be purchased separately?
FAQ ID -1214
Can RNAi experiments be performed in Drosophila?
FAQ ID -436
Can RNA purified from formalin-fixed, paraffin-embedded (FFPE) tissue be used with the QuantiTect Whole Transcriptome Kit?
FAQ ID -1583
Can RNA isolated with the RNeasy Microarray Tissue Mini Kit also be used in downstream applications other than microarray analysis?
FAQ ID -2191
Can REPLI-g WGA be used to amplify cDNA if the cDNA is dsDNA (double-stranded DNA)?
FAQ ID - 3686
Can reagent from the PAXgene Blood ccfDNA Tube flow back into the patient’s arm?
FAQ ID - 3629
Can QuantiTect Primer Assay Plates be ordered offline via e-mail or fax?
FAQ ID -2112
Can QuantiFast Probe Assays be ordered offline via e-mail or fax?
FAQ ID - 3278
Can QuantiFast Probe Assays be ordered offline via e-mail or fax?
FAQ ID -2368
Can QuantiFast PCR Kits be used on real-time PCR instruments without fast cycling options?
FAQ ID -1428
Can QIAzol aqueous phase be used with the gDNA eliminator columns in RNeasy Plus mini kit?
FAQ ID -3151
Can QIAseq FastSelect Plant or QIAseq FastSelect Yeast be combined with FastSelect 5S/16S/23S?
FAQ ID -147915
Can QIAquick Kits be used to clean up RNA samples?
FAQ ID -490
Can QIAGEN's Taq- and HotstarTaq DNA Polymerases be used for cycle sequencing?
FAQ ID -741
Can QIAGEN rotors used with the centrifuges 4C and 4KC also be used with 4-16 and 4-16K models?
-15
Can QIAcube protocol runs be interrupted, and subsequently be continued to completion?
FAQ ID -1414
Can QIAcube Connect MDx protocol runs be interrupted, and subsequently be continued to completion?
148735
Can PyroMark Gold reagents be vortexed?
FAQ ID -2844
Can purified RNA be used with the miScript Single Cell qPCR Kit?
FAQ ID - 3566
​
Can purification columns from other suppliers be processed on the QIAcube?
FAQ ID -1406
Can purification columns from other suppliers be processed on the QIAcube Connect?
FAQ ID - 141487
Can purification columns from other suppliers be processed on the QIAcube Connect MDx?
148728
Can protocol runs on the QIAsymphony SP be interrupted and resumed?
FAQ ID -1912
Can proteins be extracted from PAXgene Tissue fixed specimens?
FAQ ID - 3616
Can Phoenix Hot Start Taq utilize cDNA as template for PCR?
FAQ ID - 3900
Can phenol/chloroform be placed in the MaXtract tube for extraction at a later time?
FAQ ID -1299
Can PCR fragments generated with the EasyXpress Linear Template Kit be cloned into the pIX4.0 vector of the EasyXpress Protein Synthesis Insect Kit?
FAQ ID -1078
Can PCR fragments be normalized for the SeqTarget System without a previous purification step?
FAQ ID -2246
Can PCR fragments be normalized for the SeqTarget System after purification with QIAquick?
FAQ ID -2240
Can PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue be used for in situ hybridization?
FAQ ID -2529
Can PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue be used for in situ hybridization?
FAQ ID -3042
Can PAXgene Blood DNA Tubes be stored at higher than room temperature (18–25°C)?
FAQ ID - 3509
Can other QIAGEN buffers from non-UCP kits (e.g. AW1 instead of AUW1) be used with the QIAamp UCP DNA Micro Kit (cat. no. 56204)?
FAQ ID - 3717
Can other probe technologies besides TaqMan® be used with the Type-it Fast SNP Probe PCR Kit?
FAQ ID -2058
Can Ni-NTA resins be used to purify protein with an internal His-tag?
FAQ ID -496
Can negative crossing threshold (CT) values be negative when using the KRAS and EGFR PCR kits?
FAQ ID -2300
Can multiple QIAxcel Systems be run from one computer?
FAQ ID -1860
Can mitochondrial DNA be isolated with the PAXgene Blood DNA System?
FAQ ID - 3498
Can mitochondria separated with the Qproteome Mitochondria Isolation Kit be used for downstream DNA isolation?
FAQ ID -1188
Can miScript miRNA qPCR Assays detect single nucleotide difference between miRNAs?
FAQ ID -2723
Can miScript miRNA qPCR Assays be used for pre-miRNA detection?
FAQ ID -2724
Can miRNA sequencing libraries be prepared from total RNA isolated from heparinized plasma samples?
FAQ ID - 3661
Can miRNA and other RNAs smaller than 200 nucleotides be isolated with the AllPrep DNA/RNA Micro Kit or RNeasy Plus Micro Kit?
FAQ ID -1749
Can miRNA and other RNAs smaller than 200 nucleotides be isolated with the AllPrep DNA/mRNA Nano Kit?
148723
Can MinElute 96 UF PCR be used to purify PCR samples which contain mineral oil?
FAQ ID -360
Can membrane proteins be expressed with QIAgenes expression kits?
FAQ ID -2047
Can MaXtract tubes be autoclaved prior to use?
FAQ ID -1305
Can lyophilized plant material be used for DNA extraction on the BioSprint 96?
FAQ ID -772
Can less than 10 ng RNA be used with the QuantiTect Whole Transcriptome Kit?
FAQ ID -1590
Can I use your EpiTect One-Day kit with transcription factors?
FAQ ID -2752
Can I use your EpiTect ChIP system in a RNA study?
FAQ ID -2748
Can I use water to elute the RNA in the PAXgene Bone Marrow RNA Kit?
FAQ ID -2512
Can I use water for elution from the QIAprecipitator of the HiSpeed Plasmid Kits?
FAQ ID -306
Can I use Tungsten Carbide beads for RNA extraction protocols using QIAGEN Kits?
FAQ ID -3053
Can I use total RNA for the miRNA PCR Arrays or Assays?
FAQ ID -2726
Can I use the Type-it CNV kits and Reference Assays for CNV studis in mice?
FAQ ID -2997
Can I use the SureSilencing shRNA Plasmids with primary cells or macrophages or for injection into live animals?
FAQ ID -2902
Can I use the RNeasy Plus Universal Mini Kit for all types of tissue?
FAQ ID -2338
Can I use the RNeasy Mini Kit or RNeasy 96 Kit with fewer than 100 cells?
FAQ ID -372
Can I use the RNeasy MinElute Cleanup Kit to clean up my in vitro transcription reaction?
FAQ ID -429
Can I use the RNeasy Microarray Tissue Mini Kit for all tissue types?
FAQ ID -2190
Can I use the RNeasy Fibrous Tissue Mini Kit (cat. no. 74704) to extract RNA from fibrous plant material?
FAQ ID - 3521
Can I use the QuantiTect Multiplex PCR Kits on the Roche LightCycler systems with TaqMan® probes or QuantiTect Assays?
FAQ ID -717
Can I use the QuantiMIZE system for determination of gDNA quality for non-multiplex PCR enrichment methods (ex. Hybridization) (cat. No. NGQA-002, NGQC-100Y)?
FAQ ID - 3531
Can I use the QIAquick PCR Purification Kit for restriction enzyme cleanup?
FAQ ID -130
Can I use the QIAGEN deparaffinization solution with the IDH 1/2 RGQ PCR kit?
FAQ ID -3100
Can I use the QIAcuity Nanoplate in more than one runs?
3781
Can I use the PhosphoProtein Purification Kit to purify phosphorylated peptides of around 1 kD size?
FAQ ID -778
Can I use the PhosphoProtein Purification Kit for the isolation of phosphorylated proteins from yeast?
FAQ ID -838
Can I use the PAXgene Blood DNA Tube to collect blood from animals?
FAQ ID - 3504
Can I use the Microbial DNA-Free Water and Microbial qPCR Mastermix if they have been opened more than 3 times?
FAQ ID — 3398
Can I use the lysate from the CGT Viral Vector Lysis kit and combine it with another PCR system?
FAQ ID - 3979

Can I use the IC RNA of the QIAprep& Viral RNA UM Kit?
FAQ ID - 3895
Can I use the EZ2 AllPrep DNA/RNA FFPE kit to extract DNA/RNA from fresh frozen tissue?
FAQ ID - 3953
Can I use the default PCR program to run EpiTect Methyl qPCR Arrays or Assays?
FAQ ID -2745
Can I use the CGT dPCR assays for the titer determination of RNA viruses?
FAQ ID - 3869
Can I use TaqMan® Copy Number Assays from LIFE Technologies in combination with the Type-it CNV Probe kit?
FAQ ID -2999
Can I use stained slide materials with IDH 1/2 RGQ PCR kit?
FAQ ID -3101
Can I use specific primers with the FastLane Kits?
FAQ ID -3078
Can I use sample volumes other than 300 µl?
3801
Can I use ROX as passive reference dye with the SARS-CoV-2 Neo assay?
FAQ ID - 3894
Can I use RNAiFect for co-transfection of plasmid DNA and siRNA?
FAQ ID -515
Can I use REPLI-g for SNP Genotyping?
FAQ ID -700
Can I use QIAquant 96 Software to analyze experiments carried out on QIAquant 384?
153950
Can I use QIAprep Miniprep kits for low-copy plasmids and cosmids?
FAQ ID -127
Can I use QIAGEN's pQE vectors as templates in the EasyXpress Biotin Labeling Kits?
FAQ ID -874
Can I use PyroMark Q48 for diagnostics?
FAQ ID - 3722
Can I use plasma from underfilled PAXgene Blood ccfDNA Tubes?
FAQ ID - 3638
Can I use Omniscript or Sensiscript RT's at a higher temperature?
FAQ ID -116
Can I use my own siRNA design?
FAQ ID -2897
Can I use my own primers for REPLI-g WGA to amplify a specific chromosomal region?
FAQ ID -712
Can I use my own pipeline to analyze results from my EpiTect Hi-C experiments?
FAQ ID - 143078
Can I use my own cycling conditions with the QIAGEN Multiplex PCR Kit?
FAQ ID -287
Can I use my gene-specific primers with the FastLane Cell cDNA Kit and the QuantiTect Reverse Transcription Kit?
FAQ ID -812
Can I use MspI or HpaII restriction enzymes from other suppliers?
FAQ ID - 3975
Can I use more than 4 µl FastLane lysate in subsequent real-time RT-PCR?
FAQ ID -1480
Can I use more than 10ng DNA for the PCR reaction for the Pyro kits?
FAQ ID - 3276
Can I use more than 10ng DNA for the PCR reaction for the Pyro kits?
FAQ ID -2379
CAn I use milk or other liquids with the DNeasy mericon Food kit? What volume would I use?
FAQ ID - 3350
Can I use LyseBlue with R.E.A.L. Prep 96, QIAwell Ultra, or QIAprep 96 Turbo Miniprep Kits for the BioRobot systems?
FAQ ID -863
Can I use lysates or tissue homogenates with the ELISArray kits?
FAQ ID -2905
Can I use Internal Control as overall control?
FAQ ID - 3996
Can I use HEPES buffer instead of phosphate in my Ni-NTA column?
FAQ ID -291
Can I use genomic DNA from fixed samples with qBiomarker Copy Number PCR Arrays?
FAQ ID — 3416
Can I use fluorescence microscopy to assess the transfection efficiency of the SureSilencing shRNA Plasmids with GFP in my model cell line of interest?
FAQ ID -2894
Can I use fluorescence microscopy to assess shRNA plasmid transfection efficiency in my model cell line of interest, when using a vector expressing a GFP reporter gene?
FAQ ID -2782
Can I use ethanol instead of isopropanol for DNA precipitation when using HiSpeed Plasmid Kits?
FAQ ID -354
Can I use DNA isolated from an AllPrep DNA/RNA Kit with qBiomarker Copy Number PCR Arrays and Assays
FAQ ID — 3425
Can I use cells that have been previously crosslinked and frozen (e.g., for a ChIP or ChIP-seq experiment) as input material for the EpiTect Hi-C protocol?
FAQ ID - 143069
Can I use carrier RNA with the RNeasy UCP Micro Kit (cat. no. 73934)?
FAQ ID - 3716
Can I use blood, cells or bacteria with the QIAamp Fast DNA Tissue Kit?
FAQ ID - 3587
Can I use BioMag particles with any specific primary antibody?
FAQ ID -273
Can I use any type of DNA extraction method to purify template DNA for amplification using the REPLI-g Mitochondrial DNA Kit?
FAQ ID -1321
Can I use another lysis buffer than the one supplied in the PhophoProtein Purification Kit?
FAQ ID -645
Can I use amplified genomic DNA with qBiomarker Copy Number PCR Arrays?
FAQ ID — 3415
Can I use a USB drive to transfer protocols to BioSprint?
FAQ ID -3072
Can I use a QIAfilter Cartridge for purifying large plasmids?
FAQ ID -121
Can I use a partial MinElute 96 UF plate?
FAQ ID -355
Can I use a different elution buffer?
FAQ ID - 3847
Can I use a different brand blood collection set with the PAXgene Blood ccfDNA Tube?
FAQ ID - 3630
Can I use a custom master mix instead of a QIAGEN master mix?
3777
Can I use a centrifuge instead of vacuum when using the QIAquick 96 PCR Purification Kit?
FAQ ID -293
Can I use a bar code scanner or reader on the QIAgility?
FAQ ID -3067
Can I use a 384 well plate for the KRAS and EGFR PCR kits?
FAQ ID -2296
Can I upgrade my QIAquant 96 2plex instrument to QIAquant 96 5plex or modify the set of color channels?
153928
Can I transform the Cignal Reporter Assays?
FAQ ID -2762
Can I transfer my assays from PyroMark Q24/Q96 to PyroMark Q48 Autoprep?
FAQ ID - 3723
Can I substitute the endonuclease used in the Hi-C Digestion step with another endonuclease?
FAQ ID - 143070
Can I store the QIAseq Beads of the QIAseq Targeted DNA Panel at -20°C?
FAQ ID - 3711
Can I store cDNA generated by the miScript System?
FAQ ID -1602
Can I store agarose gel slices containing DNA for gel extraction at a later point?
FAQ ID -313
Can I stop saliva collection before the fill line is reached?
3806
Can I still reorder siRNA that has been removed from the GeneGlobe data base?
FAQ ID -1668
Can I still perform sequencing if my miRNA sequencing library concentrations are too low to obtain a 4 nM library?
FAQ ID - 3666
Can I stabilize RNA in bacteria grown on solid media or substrate using the RNAprotect Bacteria Reagent?
FAQ ID -615
Can I stabilize adipose tissue using RNAprotect Tissue Reagent prior to RNA isolation?
FAQ ID -856
Can I shorten the activation time for the HotStarTaq DNA Polymerase?
FAQ ID -565
Can I select HP Validated and HP GenomeWide siRNAs for FlexiTube?
FAQ ID -1658
Can I select FITC labeled cells with BioMag?
FAQ ID -278
Can I see on the QIAcuity Software Suite report file if the VPF (Volume Precision Factor) has been used or not?
3770
Can I see error codes on the instrument touchscreen?
3763
Can I scale up reagents if I want to use more sample with the MagAttract HMW DNA Kit (cat. no. 67563)?
FAQ ID - 3448
Can I run life science and custom protocols on a QIAcube Connect MDx?
148745
Can I run less than 15 samples on the BioSprint 15 instrument?
FAQ ID -621
Can I reuse the Ni-NTA Agarose and Ni-NTA Superflow resins?
FAQ ID -802
Can I reliably detect intermediately methylated DNA with EpiTect Methyl qPCR Array technology?
FAQ ID -2738
Can I reinstall the PyroMark Q24 software on my new computer or following an operating system upgrade, or do I need to purchase a new license?

FAQ ID - 3473

Can I reduce the reaction time to e.g., 30 minutes, when using the REPLI-g UltraFast Mini Kit?
FAQ ID -1326
Can I reduce the amount of starting material below 10 ng when using the REPLI-g UltraFast Mini Kit?
FAQ ID -1325
Can I reclone the insert sequences from the SureSilencing shRNA Plasmids into a viral-based vector?
FAQ ID -2896
Can I re-amplify whole genome amplification product?
FAQ ID -670
Can I purchase Phi29 DNA polymerase only?
FAQ ID -708
Can I program my own protocols for the QIAsymphony SP?
FAQ ID -1904
Can I program my own protocols for the QIAcube?
FAQ ID -1405
Can I program my own protocols for the QIAcube Connect?
FAQ ID - 141486
Can I program my own protocols for the QIAcube Connect MDx?
148727
Can I prepare a dPCR reaction directly in QIAcuity Nanoplate?
3774
Can I perform the IDH 1/2 RGQ PCR test with slides containing less than 40% tumor cells?
FAQ ID -3098
Can I perform in-vitro transcription with the pDrive Cloning Vector from the QIAGEN PCR Cloning Kit?
FAQ ID -332
Can I order the nucleotides from PyroMark Gold Reagents separately?
FAQ ID -2827
Can I order Alexa Fluor labeled controls with FlexiPlate siRNA?
FAQ ID -1369
Can I optimize a dPCR assay on the QIAcuity using gradient temperature?
FAQ ID - 3783
Can I operate QIAquant 96 instrument using touchscreen and computer simultaneously?
153951
Can I obtain the sequence of the RT² qPCR Primer Assay that I purchased?
FAQ ID -2709
Can I multiplex the Microbial DNA qPCR Assays?
FAQ ID — 3408
Can I measure virulence factor gene expression?
FAQ ID — 3405
Can I measure antibiotic resistance gene expression?
FAQ ID — 3404
Can I manually set the threshold line?
FAQ ID -2702
Can I make stable cell lines with the Cignal Reporter Assays?
FAQ ID -2763
Can I make a master mix with QuantiProbes, primers, and all other reaction components and store it for later use?
FAQ ID -584
Can I increase the annealing temperature recommended for QuantiTect Primer Assays used with the QuantiTect SYBR Green PCR Kits?
FAQ ID -1146
Can I homogenize samples in QIAzol using the QIAshredder?
FAQ ID -501
Can I follow the reaction setup for probe-based assays described in Appendix D of the FastLane Cell RT-PCR Handbook using 384-well plates?
FAQ ID -1482
Can I eliminate RNase A from buffer P1 for my plasmid preparation to obtain RNase-free DNA for in-vitro transcription?
FAQ ID -366
Can I do whole genome amplification from mitochondrial DNA using REPLI-g?
FAQ ID -668
Can I do whole genome amplification from brain tissue using REPLI-g?
FAQ ID -669
Can I do singleplex PCR with the QuantiFast Probe Assays?
FAQ ID -2362
Can I do 4-plex real-time PCR, on the ABI PRISM 7000, 7700, or 7900 using the QuantiTect Multiplex PCR Kit?
FAQ ID -716
Can I directly transfect SureSilencing shRNA Plasmids into my cells?
FAQ ID -2899
Can I connect more than one QIAquant instrument to the same computer?
153953
Can I combine your CGT dPCR assays with custom designed assays?
FAQ ID - 3861
Can I clean up my DNase treated RNA samples using RNeasy columns?
FAQ ID -286
Can I change the language on my QIAsymphony SP system?
FAQ ID - 3595
Can I buy the RNeasy Mini columns, RNeasy MinElute columns, RNeasy Midi columns or the RNeasy Maxi columns separately?
FAQ ID - 3388
Can I buy the gDNA eliminator plates supplied in your RNeasy Plus 96 kit separately?
FAQ ID - 3389
Can I buy the gDNA eliminator columns supplied in your RNeasy Plus kits separately?
FAQ ID - 3390
Can I buy the 1 ml Bead Plates from the DNeasy UltraClean 96 Microbial Kit (384) separately?
FAQ ID - 3726
Can I buy QIAquick and MinElute columns separately?
FAQ ID -2460
Can I ask QIAGEN to upgrade my current QIAcube Connect Life Science instrument to make it an MDx instrument?
148744
Can I analyze my Next Generation Sequencing (NGS) samples on the QIAxcel for Quality control?
FAQ ID - 3724
Can I amplify mitochondrial DNA directly from whole blood or cells using the REPLI-g Mitochondrial DNA Kit?
FAQ ID -1319
Can I amplify from plasma or serum samples using the REPLI-g Mini/Midi kits?
FAQ ID -671
Can I also screen large constructs like BACs, fosmids, and cosmids using the QIAGEN PlasmidAmp Kit?
FAQ ID -1337
Can I adjust the ROX concentration in the QuantiFast master mix?
FAQ ID -1427
Can I add bleach to the sample preparation waste?
FAQ ID - 3505
Can HP Validated siRNAs be ordered on the FlexiPlate?
FAQ ID -1367
Can HiPerFect HTS Reagent be used for transfecting shRNAs?
FAQ ID -2559
Can HiPerFect HTS be used for miRNA?
FAQ ID -2561
Can Heparin blood be used?
3834
Can glycosylated proteins be expressed using the EasyXpress Insect Kit II?
FAQ ID -1227
Can Glutathione Superflow resin be reused?
FAQ ID -2171
Can FTA paper for dried blood spots be used with REPLI-g?
FAQ ID -685
Can frozen samples be used with the MagAttract 96 cador Pathogen Kit?
FAQ ID -2070
Can frozen sample material pulverized in liquid nitrogen be directly added to MaXtract tubes?
FAQ ID -1301
Can frozen blood be used for whole genome amplification with REPLI-g?
FAQ ID -794
Can frozen blood be used for the procedure?
3832
Can FlexiTube siRNA or FlexiTube GeneSolution products be ordered in solution?
FAQ ID -1667
Can FlexiPlate siRNAs be ordered in plates other than the standard NUNC 96 well V bottom plates?
FAQ ID -1458
Can FlexiPlate siRNA be ordered in solution on dry-ice?
FAQ ID -1474
Can fast cycling be carried out with QuantiFast Probe PCR Kits and FRET probes?
FAQ ID -1433
Can DyeEx be used to clean up labeled DNA other than sequencing templates?
FAQ ID -137
Can DNase digestion be performed on the RNeasy 96 plate during the RNA purification part of the Allprep DNA/RNA 96 procedure?
FAQ ID -2000
Can DNA yields be increased by prolonging incubation with proteinase K in Buffer ATL?
FAQ ID -2032
Can DNA isolated with MaXtract tubes be used for downstream applications such as restriction digestion, labeling, blotting, PCR, and automated sequencing?
FAQ ID -1303
Can Dithiothreitol (DTT) be used instead of BME in Buffer RLT Plus of RNeasy Plus Mini Kit?
FAQ ID -3125
Can different incubation times be used for the 2 hour amplification step in the QuantiTect Whole Transcriptome protocol?
-8
Can different amounts of siRNA be ordered on one FlexiPlate?
FAQ ID -1473
Can degraded RNA be used with the QuantiTect Whole Transcriptome Kit?
FAQ ID -1586
Can data be saved at once with the QIAxcel System after analysis of all samples in a 96-well PCR plate?
FAQ ID -1861
Can data be analyzed while the run is proceeding? Can data be analyzed from a previous experiment while another experiment is running?
153948
Can data be analyzed on the Rotor-Gene Q while a run is in progress?
FAQ ID -2091
Can customers use their own DNA size marker with the QIAxcel System and if yes, what concentration should be used?
FAQ ID -1841
Can cells stabilized with RNAprotect Cell Reagent be used for cell sorting by flow cytometry (FACS)?
FAQ ID -1216
Can cDNA synthesized with the miScript Plant RT Kit be used in real-time PCR with TaqMan® MicroRNA Assays?
FAQ ID - 3445
Can cDNA prepared with the QuantiTect Whole Transcriptome Kit be reampliied using REPLI-g Kits?
FAQ ID -1611
Can cDNA prepared by any method be used as starting material in the ligation reaction of the QuantiTect Whole Transcriptome Kit?
FAQ ID -1589
Can Buffers N3 and P3 be used interchangeably?
FAQ ID -310
Can Buffer R1 of the R.E.A.L. Prep 96 Plasmid Kit be replaced with Buffer P1?
FAQ ID -823
Can bone marrow from leukemia patients with elevated numbers of white blood cells be processed with the PAXgene Bone Marrow RNA System?
FAQ ID -2510
Can bone marrow aliquots other than 2 mL be pipetted into the PAXgene Bone Marrow RNA Tube?
FAQ ID -2503
Can BioSprint 96 be connected to LIMS?
FAQ ID -3070
Can BioMag be used for positive and negative selection?
FAQ ID -269
Can BAC DNA prepared with the R.E.A.L. Prep 96 Plasmid Kit be used for high-throughput sequencing?
FAQ ID -643
Can assays for 20 and 100 reactions be ordered on the same QuantiTect Primer Assay Plate?
FAQ ID -2108
Can amplification with the QuantiTect Whole Transcriptome Kit be extended to more than 8 hours to improve cDNA yields?
FAQ ID -1608
Can Allprotect Tissue Reagent be used to stabilize RNA, DNA and proteins in cells, bacteria, yeast, and plants?
FAQ ID -1574
Can acetone be used for precipitation of protein from Buffer RLT lysates generated with RNeasy Kits?
FAQ ID -1164
Can a QIAquick Gel Extraction Kit be used to obtain RNA from a formaldehyde gel?
FAQ ID -133
Can a phenol-chloroform ratio different from 1:1 also be used with MaXtract Low and High Density?
FAQ ID -1309
Can 4-plex, real-time PCR be performed on the ABI PRISM 7000, 7700, or 7900 using the QuantiFast Multiplex PCR Kit?
FAQ ID -1978
Can 2 µl reaction volumes be used with QuantiFast PCR Kits?
FAQ ID -1440
Can 0.1 ml and 0.2 ml tubes used on the Rotor-Gene Q be labeled?
FAQ ID -2082
Buffer SB1 in the GeneRead Size Selection Kit turned yellowish from a new kit. Can I still use it?
FAQ ID - 3562
At which step in the REPLI-g FFPE protocol should PicoGreen quantification be done?
FAQ ID -1758
At what temperature can I perform the NexTal Cubic Phase experiment and incubate the plate?
FAQ ID -2415
At what temperature can I perform the experiment with the NexTal Cubic Phase kits?
FAQ ID -2323
At what point can I stop and freeze my samples when using ChIP?
FAQ ID -2754
Aside from the OTV test, what other maintenance should be done for the Rotor-Gene Q?
FAQ ID -3181
As part of my RT procedure I routinely heat an aliquot of the eluate prior to the RT reaction. Do I still need to heat the whole eluate after the RNA preparation?
FAQ ID - 3468
Are your FlexiTube siRNAs preferentially designed to any particular region of the target gene?
FAQ ID -1091
Are you able to supply a Certificate of Analysis for the KRAS and EGFR PCR kits?
FAQ ID -2301
Are we able to customize protocol on the EZ2 Connect?
FAQ ID - 3850
Are there two vectors for protein expression using QIAgenes in insect or mammalian cells?
FAQ ID -2048
Are there stopping points in the Hi-C workflow?
FAQ ID - 143071
Are there specific recommendations for performing RT-PCR on RNA isolated from paraffin-embedded samples?
FAQ ID -828
Are there specific recommendations for performing RT-PCR on RNA isolated from paraffin-embedded samples?
FAQ ID - 3955
Are there specific recommendations for performing RT-PCR on RNA isolated from paraffin-embedded samples?
FAQ ID - 3944
Are there special requirements for flow sorting?
FAQ ID - 3546
Are there special requirements for flow sorting?
FAQ ID - 3558
Are there special considerations for phlebotomy when using the PAXgene Blood ccfDNA Tube (blood collection set, discard tube, positioning of the tube, issues with short draws, etc.)?
FAQ ID - 3635
Are there recommendations for sensitive detection of underrepresented fragments using the Type-it Mutation Detect PCR Kit?
FAQ ID -2066
Are there problems with the default threshold setting on the Applied Biosystems 7500 when using QuantiFast Probe PCR Kits?
FAQ ID -1702
Are there other options for paraffin removal?
3754
Are there other options for paraffin removal?
FAQ ID - 3949
Are there other options for paraffin removal?
FAQ ID - 3946
Are there components in biological samples that can inhibit the REPLI-g reaction?
FAQ ID -667
Are there any unique elements that need to be incorporated into an shRNA expression vector?
FAQ ID -2772
Are there any specific cleaning solutions recommended for the QIAsymphony SP?
FAQ ID -1913
Are there any specific cleaning solutions recommended for the QIAcube?
FAQ ID -1416
Are there any changes in the procedure using the Strep-Tactin Superflow Plus resin with the higher capacity over the standard matrix?
FAQ ID -2168
Are there any accessories required for sample disruption with the TissueLyser LT?
FAQ ID -2551
Are the QuantiFast Probe Assays wet-lab validated?
FAQ ID -2375
Are the QuantiFast Probe Assays hydrolysis or FRET probes?
FAQ ID - 3271
Are the QuantiFast Probe Assays hydrolysis or FRET probes?
FAQ ID -2363
Are the QIAwave kits based on a different chemistry than the legacy kits?
FAQ ID - 3990

Are the QIAshredder columns included in various QIAGEN kits the same?
FAQ ID - 3624
Are the QIAseq miRNA NGS 5’ Adapter and QIAseq miRNA NGS RT Initiator compatible with the QIAseq miRNA UDI kits?
FAQ ID - 3836
Are the QIAprep 96 Plus Miniprep Kit or the QIAGEN Plasmid Plus 96 Miniprep Kit suited for short hairpin vectors?
FAQ ID -2318
Are the QIAprecipitator Midi and Maxi Modules of the HiSpeed Plasmid Kits interchangeable?
FAQ ID -308
Are the protocols from the BioSprint software compatible with the KingFisher software and vice versa?
FAQ ID -770
Are the PCR products generated with CRISPR-Q Custom PCR Assays only applicable to analysis of editing efficiency by Sanger sequencing?
3794
Are the PAXgene Blood DNA Tubes sterile?
FAQ ID - 3487
Are the new EZ1 RNA Kits (cat. no. 958034; 959034; 956034) compatible with the previous EZ1 RNA Card (cat. no. 9015590) or EZ1 RNA Universal Tissue Card (cat. no 9016384)?
FAQ ID -1025
Are the multicopy reference assays for Type-it CNV PCR +qC Kits and the qBiomarker assays the same?
FAQ ID -3000
Are the miRNA Target Protector & miRNA mimic bench tested? Any guarantee?
FAQ ID - 3186
Are the Microbial DNA qPCR Assays wet-lab verified?
FAQ ID — 3406
Are the layouts for both 96- and 384-well QuantiTect Primer Assay Plates visible when ordering online?
FAQ ID -2111
Are the Internal Controls used in the QuantiFast Pathogen PCR +IC Kit and the QuantiFast Pathogen RT-PCR +IC Kit homologous to a known target?
FAQ ID -2598
Are the CRISPR-Q PCR Assays and the CRISPR-Q Sanger Primers on GeneGlobe validated?
3793
Are the CoralLoad dyes in the HotStarTaq Plus PCR Buffer visible when loading small amounts of PCR product onto a gel?
FAQ ID -1051
Are the columns of the QIAquick PCR Purification-, Gel Extraction-, and Nucleotide Removal Kit interchangeable?
FAQ ID -577
Are the columns of the MinElute Reaction Cleanup-, Gel Extraction-, and PCR Purification Kit identical?
FAQ ID -581
Are the CGT dPCR assays also compatible with qPCR?
FAQ ID - 3981

Are the buffers in the RNeasy QIAcube kit delivered in QIAcube buffer bottles?
FAQ ID -2334
Are the buffers in the Ni-NTA Fast Start Kit the same as the ones for use with Ni-NTA purchased separately?
FAQ ID -791
Are the BioSprint 15/96 instruments stand-alone workstations?
FAQ ID -620
Are the assays species-specific?
FAQ ID — 3400
Are the assays also compatible with qPCR?
FAQ ID - 3871
Are the 96- and 384-sample blocks interchangeable?
153924
Are TaqMan® Gene Expression Assays from Applied Biosystems compatible with the Rotor-Gene Multiplex PCR Kit?
FAQ ID -2131
Are TaqMan® Gene Expression Assays from Applied Biosystems compatible with Rotor-Gene Probe Kits?
FAQ ID -2126
Are TaqMan® Gene Expression Assays from Applied Biosystems compatible with QuantiFast Probe PCR Kits?
FAQ ID -1452
Are special kits and protocols required to isolate biomolecules from PAXgene Tissue fixed, cryo-embedded (PFCE) tissue?
FAQ ID - 3614
Are scoring sheets available for each crystallization condition Screening Suite?
FAQ ID -1125
Are samples that are contaminated with high molecular weight DNA (e.g. from a paramedic or police officer) a problem with REPLI-g amplification?
FAQ ID -683
Are run reports and/or log files available on the QIAcube?
148733
Are run reports and/or log files available on the QIAcube?
FAQ ID -1412
Are Rotor-Gene Kits compatible with reaction setup using the QIAgility instrument?
FAQ ID -2116
Are RNeasy spin columns sold separately?
FAQ ID -159
Are QuantiTect PCR +UNG Kits available in bulk format (e.g., 25 ml master mix)?
FAQ ID -2136
Are QuantiFast Multiplex PCR Kits available in trial sizes?
FAQ ID -1981
Are QR codes recognized by QIAsymphony?
FAQ ID - 3625
Are QIAshredder spin columns delivered with the RNeasy Mini QIAcube Kit?
FAQ ID -2333
Are QIAprep and QIAquick Spin columns interchangeable?
FAQ ID -311
Are QIAGEN monoclonal Anti His Antibodies glycosylated?
FAQ ID -334
Are QIAGEN EZ Competent Cells of the QIAGEN PCR Cloning Kit dam+ or dam-?
FAQ ID -638
Are QIAcube reagent bottles (cat. no. 990393) autoclavable? What type of plastic are they made of?
FAQ ID - 3369
Are QIAamp DNA isolation kits suitable for apoptosis studies?
FAQ ID -149
Are protocols also available for other instruments (e.g., Hamilton Star and Tecan Fluent)?
3797
Are protein modifications such as glycosylation problematic when using the Qproteome Plasma Membrane Protein Kit?
FAQ ID -1196
Are probes other than TaqMan® probes, such as FRET probes, compatible with fast cycling with the Rotor-Gene Multiplex PCR Kit?
FAQ ID -2130
Are primers available that only detect mitochondrial DNA encoded genes and not nuclear genomic DNA encoded genes?
FAQ ID -2680
Are plant miScript Primer Assay designs wet-bench validated?
FAQ ID - 3440
Are peak heights of Q48 comparable to other PyroMark instruments?
FAQ ID - 3692
Are PAXgene Tissue Containers suitable for long term-storage at freezing temperatures?
FAQ ID -3030
Are PAXgene Blood ccfDNA Tubes sterile?
FAQ ID - 3636
Are pathogens inactivated in human saliva samples collected with the PAXgene Saliva Collector?
3819
Are panels and bin sets available for use with GeneMapper ID and GeneMapper ID-X?
FAQ ID - 3267
Are Northern Blots sensitive enough to detect siRNA-induced gene silencing?
FAQ ID -403
Are miScript primer assays bench-validated?
FAQ ID -3145
Are miScript Primer Assays available for plant mature miRNAs?
FAQ ID - 3439
Are miScript Primer Assay included in miScript miRNA PCR Arrays the same primers as the miScript Primer Assays available in individual tubes?
FAQ ID - 3441

 

Are MaXtract tubes siliconized?
FAQ ID -1298
Are immunohistochemistry (IHC) assays developed for formalin-fixed, paraffin-embedded (FFPE) tissues compatible with PAXgene Tissue fixed, paraffin-embedded (PFPE) tissues?
FAQ ID - 3609
Are immunohistochemistry (IHC) assays developed for formalin-fixed, paraffin-embedded (FFPE) compatible with PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?
FAQ ID -3040
Are HEPES, MOPS and PIPES buffers compatible with QIAGEN's PhosphoProtein Purification kit?
FAQ ID -482
Are FlexiTube siRNA and FlexiTube GeneSolution also available for rat siRNAs?
FAQ ID -1664
Are FlexiPlate siRNAs available at a higher final concentration than 10 µM?
FAQ ID -1476
Are EpiTect Methyl qPCR Arrays or Assays applicable to FFPE samples?
FAQ ID -2733
Are EasyXtal 15-Well Tools stackable?
FAQ ID -1116
Are Centromeres and Telomeres amplified using REPLI-g WGA?
FAQ ID -702
Are Buffer PB of the QIAquick PCR Purification Kit and Buffer QG of the QIAquick Gel Extraction Kit interchangeable?
FAQ ID -786
Are BioMag particles stable in other solvents?
FAQ ID -267
Are any of the miScript Single Cell qPCR Kit components interchangeable with miScript II RT Kit, miScript Plant RT Kit, miScript PreAMP PCR Kit, or miScript Microfluidics PreAMP Kit components?
FAQ ID - 3569
Are any of the miScript II RT Kit components interchangeable with the miScript Plant RT Kit components?
FAQ ID - 3436
Are any components of the QIAseq miRNA Library Kit interchangeable with components from other QIAGEN kits?
FAQ ID - 3662
Are all QIAxcel Cartridge Kits compatible with QIAxcel Connect?
FAQ ID - 3856
Are all 3 components of the SeqTarget System required or can a different Long Range PCR Kit and primers be used?
FAQ ID -2234
Apart from the antibody itself, does the Penta-His Biotin Conjugate solution contain any protein?
FAQ ID -359
Anything important when starting the cycler run?
FAQ ID - 3888
Any data on the fidelity of the REPLI-g MDA technique?
FAQ ID -707
An immediate subsequent AllPrep DNA/RNA FFPE run does not start right away.
FAQ ID - 3952
After using your U/A PCR Cloning Kit I can detect colonies, which are light blue, and not really white. Why is that?
FAQ ID -512
After bisulfite conversion, can the DNA be stored?
FAQ ID -2409
A white precipitate has formed in my 10x RT buffer. Is it still ok to use?
FAQ ID -216
895 - Do you have a protocol for the isolation of endotoxin-free plasmid DNA using the QIAGEN Plasmid Midi Kit?
5. What is the maximum size of the tissue sample that can be fixed in a PAXgene Tissue Container?
FAQ ID -3023
3843-How-do-I-prevent-a-drifting-baseline-in-my-pyrosequencing-pyrogram
FAQ ID - 3843
3497 - What is the expected purity and size of DNA extracted using the PAXgene Blood DNA kit?
FAQ ID - 3497
3354 - What type and amount of resin is packed into the Ni-NTA Spin columns from the Ni-NTA Spin Kit?
FAQ ID - 3354
3353 - What is the composition of the elution buffer in the Ni-NTA Fast Start Kit?
FAQ ID - 3353
3352 - What are the size ranges of Ni-NTA particles?
FAQ ID - 3352
3351 - What is the upper limit for protein size that can bind to Ni-NTA agarose resin?
FAQ ID - 3351
3345 - What is the difference between the high and low concentration EpiTect Bisulfite Conversion protocol?
FAQ - 3345
3344 - What is the difference between the EpiTect Plus DNA Bisulfite kit compared to the classic kit?
FAQ - 3344
3343 - Why is it difficult to quantify DNA using UV/Vis spectroscopy and fluorescence methods following EpiTect Bisulfite Conversion? How best do you quantify it?
FAQ - 3343
3342 - How is the unmethylated unconverted EpiTect DNA control to be used?
FAQ - 3342
3341 - How are the EpiTect DNA controls produced and tested?
FAQ - 3341
3339 - Does the miRNeasy Serum/Plasma Spike-in Control (cat n° 219610) negatively impact subsequent NGS sequencing?
3338 - Does the miRNeasy Serum/Plasma Spike-in Control (cat n° 219610) have 3'-hydroxyl and 5'-phosphate groups?
3337 - Which method is used for QIAGEN sequencing service?
3336 - Can I use the linearized DNA for Epitect Bisulfite treatment with the Epitect Bisulfite kit?
3335 - Can I use the DNA directly from enzymatic reaction for Epitect Bisulfite treatment with the Epitect Bisulfite kit (cat. no. 59104)?
3333 - When using REPLI-g, what is the purpose of the DTT added to the denaturation buffer in the cells/blood protocol?
3332 - Is there a method that can distinguish between non-amplified DNA and REPLI-g amplified DNA?
3331 - Should the REPLI-g amplified mtDNA be cleaned up before sequencing?
3330 - Is there any contamination from E. coli genomic DNA in the polymerase provided with the Repli-G Single Cell Kit?
3329 - When using REPLI-g, if the starting material is a single sorted cell, could mitochondrial DNA (mtDNA) be amplified?
3328 - Can the Sample Tubes CB and the Sample Tubes RB be used interchangeably?
3327 - How often should the o-ring for the pipettor tip-adapter be changed on the QIAcube?
3326 - Which QIAcube standard protocol might be suitable to extract RNA from saliva or from a buccal cell pellet?
3325 - What are the catalog numbers for the packing materials for the QIAcube?
3324 - Do you have a protocol for the QIAprep Spin M13 Kit on the QIAcube?

 

3323 - Do you have a protocol for the AllPrep DNA/RNA/Protein Mini Kit on the QIAcube?
FAQ ID - 3323

 

3322 - What is the concentration of the reconstituted miScript Primer Assay (100) and miScript Precursor Assay (100) tubes?
3321 - What is the purpose of the C. elegans miR-39 spike-in control in the miRNeasy Serum/Plasma kit?
3320 - What is the sensitivity of the Type-it HRM PCR Kit?
3318 - What is the preferred amplicon size for performing PCR on DNA amplified with the EpiTect Whole Bisulfitome Kit?
3318 - What guidelines should I follow in designing primers for use with the EpiTect MSP Kit?
3317 - Do you have a protocol for isolation of genomic DNA from cell cultures using the QIAamp cador Pathogen Mini Kit?
3316-What is the concentration of the reconstituted QuantiFast Probe Assay?
3315-What is the concentration of the reconstituted QuantiTect Primer Assay (200)?
3314-What is the concentration of RNase A sold separately?
3313 - What is the endotoxin level for the QIAGEN Plasmid Plus kits?
3312 - For DirectPrep 96 miniprep, is it possible to use a half plate and save the other half for later prep?
3311 - Can QuickLyse miniprep kits be used with QIAVac 24 Plus?
3310 - Which settings for the filter set Any5Dye must be adjusted before running the spectral calibration for Investigator ESSplex SE Plus Kit?
3309 - Which filter set should be used with Investigator ESSplex SE Plus Kit?
3308 - Where can I find the template files for the Investigator ESSplex SE Plus Kit?
3306 - What is the optimal sample DNA concentration for the Investigator ESSplex SE Plus Kit?
3305 - What is the minimal amount of starting material required for using the SBTexcellerator HLA Kits?
3304 - What is the maximum DNA input volume that can be used in the Investigator ESSplex SE Plus Kit?
3302 - How long can I store my purified RNA?
3301 - Do you have any information or guidelines regarding the choice of reference genes for real-time PCR?
3300 - Can isopropanol or methanol be used in place of ethanol in RNeasy kits?
3299-Can Buffer ER from the Endofree Plasmid kits be used instead of the Buffer ETR in QIAGEN Plasmid Plus kits?
3298-Will the sequence of the QuantiFast Probe Assays be provided?
3297-Why does the PCR for HLA-DRB4 result in 2 amplicons of different size?
3296-Why does the A peak reduction factor have to de set to 0.86 for the codon 61 assay in the therascreen NRAS Pyro Kit?
3295-What is the difference between SBTexcellerator HLA Core, Extended and Full Kits?
3294-How long can I store my purified DNA/RNA isolated with the AllPrep DNA/RNA FFPE kit?
3292-How do I start using the NeXtal CubicPhase kits?
3291-How do I harvest the crystals from meso phase? Are special tools needed with the NexTal Cubic Phase products?
3290-How do I analyze the data for the particular iPSC qBiomarker Arrays I have ordered?
3289-How does the QuantiFast Probe RT-PCR Plus Kit eliminate genomic DNA contamination?
3288-How does the gDNA eliminator solution of the RNeasy Plus Universal Kit work?
3286-How can I adjust the ROX concentration in the QuantiFast Probe RT-PCR Plus master mix?
3285-How are the genes in each iPSC qBiomarker PCR Array selected?
3284-For which applications can the RNA isolated with the RNeasy Plus Universal Kit also be used?
3283-Do you have any information or guidelines regarding the choice of reference genes for real-time PCR?
3277 - Can I use the RNeasy Plus Universal Mini Kit for all types of tissue?
3275 - Can I use more than 10ng DNA for the PCR reaction for my therascreen Pyro Kits?
3274 - Can I do singleplex PCR with the QuantiFast Probe Assays?
3273 - At what temperature can I perform the experiment with the NexTal Cubic Phase kits?
3272 - Are the QuantiFast Probe Assays wet-lab validated?
3270 - Are the primer sets different for the Investigator Nonaplex ESS Kit and ESSplex SE where the loci are the same?
3266 - Why are two different exon 2 forward sequencing primers used for the SBTexcellerator HLA-DRB1 Kit?
3265 - Why are there various different QuantiFast Probe Assays for my gene (previous versions, transcript variants)?
3264 - Why are the QuantiFast Probe Assays located on a single exon, thereby also detecting genomic DNA?
3263 - Why are samples centrifuged at 4°C after the addition of chloroform when using the RNeasy Plus Universal Mini Kit?
3262 - Which thermal cyclers are validated for use with the Investigator Nonaplex ESS Kit?
3261 - Which purification kits are recommended for the therascreen Pyro kits?
3260 - Which purification kits are recommended for the Pyro kits?
3259 - Which probe labels are available for the QuantiFast Probe Assays?
3258 - Which filter set and settings should be used with the Investigator Nonaplex ESS Kit?
3257 - Which deparaffinization method can I use with the AllPrep DNA/RNA FFPE Kit?
3256 - Which crystallization solutions are compatible with lipidic cubic phase crystallization?
3255 - When using the NexTal Cubic Phase products, should I work with diluted or undiluted screens?
3254 - When using the Nextal Cubic Phase products how do I know in which phase the sample is? What will I see?
3253 - What species are QuantiTect Probe Assays offered for?
3252 - What should be the concentration of my protein with the NexTal Cubic Phase products?
3249 - What is the optimal DNA volume and concentration for the Investigator Nonaplex ESS Kit?
3248 - What is the difference between sponge, cubic and lamellar phase?
3247 - What is the correct matrix to use with the Investigator Nonaplex ESS Kit?
3246 - What is the correct matrix to use with Investigator ESSplex SE Plus Kit?
3245 - What is the concentration of the primers and the probe in a reaction using the QuantiFast Probe Assays?
3244 -What is the average length of a QuantiFast Probe Assay?
3243 - What does the Investigator Nonaplex ESS Kit contain?
3241 - What are the recommended storage conditions of the QuantiFast Probe RT-PCR Plus Kit components?
3240 - What are the main differences between the qBiomarker PCR Arrays and the RT2 Profiler PCR Arrays?
3239 - What are the differences between the existing QuantiFast Probe RT-PCR Kit and the new Plus Kit?
3238 - What are important points regarding the genomic DNA wipeout buffer in the QuantiFast Probe RT-PCR Plus Kit?
3237 - I would like to order the QuantiFast Probe Assays without master mix. Is this possible?
3236 - I use cultured cells as starting material. Can I also use the RNeasy Plus Universal Kit?
3233 - Is the yield and quality of RNA and DNA purified with the AllPrep DNA/RNA FFPE Kit comparable to yield and quality of nucleic acids purified separately with other methods.
3232 - Is the RNeasy Plus Universal Mini Kit suited for adipose, brain, and other fatty tissues?
3231 - Is there a size limit to the protein I can crystallize with the NexTal Cubic Phase products?
3229 - Is it necessary to perform calibration steps for the use of the MAX dye in duplex RT-PCR experiments on different cyclers?
3228 - Is it also possible to isolate miRNA with the AllPrep DNA/RNA FFPE Kit?
3227 - In which solution can I keep my protein with the NexTal Cubic Phase kits?
3226 - How pure should the chloroform used with the RNeasy Plus Universal Mini Kit be?
3225 - How pure should my protein be for the NexTal CubicPhase kits?
3224 - How much time can be saved when using the RNeasy Mini QIAcube Kit?
3223 - How much sample material can I use for DNA/RNA purification with the AllPrep DNA/RNA FFPE Kit?
3222 - How much DNA/RNA can be isolated with the AllPrep DNA/RNA FFPE Kit?
3220 - How many PCR cycles should I use for the Investigator Nonaplex ESS Kit?
3219 - How many freeze-thaw cycles can the master mix contained in the QuantiFast Probe RT-PCR Plus Kit tolerate?
3218 - How many fragments are contained within the size standard BTO in the Investigator Nonaplex ESS Kit?
3217 - How long can I store my purified RNA?
3215 - What is the starting material for SBTexcellerator HLA Kits?
3213 - How much ethanol should be added to Buffer AP3/E (renamed to Buffer AW1) and Buffer AW (renamed to Buffer AW2) in the DNeasy Plant kit?
3212 - What is the minimum size of DNA fragment that can be isolated with QIAamp DNA Mini kit?
3211 - What is the maximum speed of the plate rotor 2 x 96 in the 4-16 or 4-16K?
3210 - Can the 4-16K Centrifuge be used with a 208 V outlet?
3206 - Can our GeneRead Library preparation kits be used with the SoLiD Platform?
3205 - Can GeneRead Library preparation kits be used on species other than human mouse and rat, for example, plant species and microbial species?
3204 - How strict is the purification cut-off with GeneRead Size Selection kit?
3203 - Is the RNeasy MinElute cleanup kit required for the GeneRead rRNA depletion kit?
3202 - Can GeneRead rRNA depletion kit be used to deplete rRNA from miRNA isolated using miRNeasy kit?
3201 - What quality of RNA can be used with the GeneRead rRNA depletion kit?
3200 - What is the Maximum/Minimum amount of RNA that can be used with GeneRead rRNA depletion kit?
3199 - Which species can be used with the GeneRead rRNA depletion kit?
3198 - What is the optimal DNA concentration for the Investigator DIPlex Kit?
3197 -Where can I find the Population Data for the Investigator HDplex kit?
3196 - What is the DIPsorter Freeware?
3195 - Where can I find the Population Data for the Investigator DIPlex kit?
3194 - What DNA fragment sizes are required for the Investigator DIPlex kit?
3193 - Where can I get an RNase-free syringe that is recommended for reconstituting QIAGEN's RNase-free DNase (cat. no. 79254)?
3192 - Is QIAGEN Protease compatible with Buffer ATL?
3191 - Is the Allprep DNA Mini Spin Column the same as the gDNA eliminator column? Can I interchange these columns?
3190 - What is the smallest elution volume for RNeasy Mini Spin column?
3188 - How many His residues are required for successful purification on Ni-NTA resins?
3187 - Why does the Ni-NTA resin turn reddish-brown after mixing with the lysates?
303 - Can I use my own lysis buffer with the DNeasy Blood & Tissue or QIAamp DNA Mini Kit?
2. Are there any special considerations for the collection of blood using PAXgene Blood DNA Tubes CE (e.g., use of a blood collection set or discard tube, positioning of the tube, issues with short draws, etc.)?
FAQ ID - 3485
回到顶部