常见问题

 

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What is MOI?
FAQ ID -2768
What file format and layout do I need to upload my data into the PCR Array Data Analysis software?
FAQ ID -2700
What does the RT² qPCR Primer Assay product information mean when it says that it recognizes another transcript of the same gene?
FAQ ID -2712
What do I need in order to set up an RNAi experiment?
FAQ ID -2757
What does a typical EpiTect Methyl qPCR Assay or Array include?
FAQ ID -2740
Why can't I find the DNA methylation qPCR Assay for my gene of interest?
FAQ ID -2743
Why did the real-time PCR yield Ct values < 12?
FAQ ID -2727
Why do I need to identify my real-time instrument model when placing my order for RT² qPCR Primer Assays?
FAQ ID -2713
What would happen if I used home-made PCR master mixes or master mixes from other manufacturers with the RT² products?
FAQ ID -2715
Which qPCR instrument should I use with your RT² qPCR Primer Assays?
FAQ ID -2714
Why are the RT² qPCR Primer Assays not designed to cross exon-intron junctions or boundaries?
FAQ ID -2710
Why should I use RT² SYBR Green Mastermix with RT² qPCR Primer Assays?
FAQ ID -2706
Will pipetting error affect the EpiTect Methyl qPCR Array results?
FAQ ID -2741
Will pipetting error affect the miRNA qPCR Assay results?
FAQ ID -2728
Why had my RT² SYBR Green Mastermix been working well in the past, but now does not seem to be?
FAQ ID -2717
What MOI should I use for my cells?
FAQ ID -2770
What RT² qPCR Primer Assays are available?
FAQ ID -2708
What transfection reagents are compatible with the Cignal Reporter Assays?
FAQ ID -2764
What is the most reliable transfection reagent for delivering shRNA plasmids and siRNA to cells in culture?
FAQ ID -2777
What are the key parameters contributing to unwanted off-target effects in an RNAi experiment?
FAQ ID -2775
What are the structures of the siRNA molecules used in RNAi studies?
FAQ ID -2760
What are the critical factors in designing the siRNA molecules to be used for RNAi studies?
FAQ ID -2759
Why is my EpiTect Methyl qPCR Assay "failed" as indicated in the QC page of the data analysis Excel file?
FAQ ID -2734
Why is the qRT-PCR reproducibility so critical when detecting gene expression knock down in an RNAi experiment?
FAQ ID -2774
Can miScript miRNA qPCR Assays be used for pre-miRNA detection?
FAQ ID -2724
Can miScript miRNA qPCR Assays detect single nucleotide difference between miRNAs?
FAQ ID -2723
Do I need an internal methylated DNA reference in the EpiTect Methyl qPCR Array?
FAQ ID -2736
Do I need the sequence specific miRNA primers in the RT step of the miRNA qPCR Assays?
FAQ ID -2725
Do you always run samples in triplicates?
FAQ ID -2703
Can you use stained (H & E) sections with RT² PreAMP cDNA Synthesis Kit?
FAQ ID -2722
Does this method with the EpiTect Methyl qPCR Array detect methylation at specific CpG dinucleotides?
FAQ ID -2737
Does QIAGEN guarantee the performance of the SureSilencing shRNA Plasmids?
FAQ ID -2787
Can I make stable cell lines with the Cignal Reporter Assays?
FAQ ID -2763
How are the primers in the EpiTect Methyl qPCR Array designed?
FAQ ID -2735
Is it acceptable to correct for transfection efficiency, when determining the level of gene expression knock down by an shRNA expression vector?
FAQ ID -2783
Should I use monoclonal or polyclonal antibodies in EpiTect ChIP assays?
FAQ ID -2747
What are the advantages of using the SureSilencing shRNA Plasmids over siRNA?
FAQ ID -2786
The pathway reporter luciferase activity values are greater than the positive control, is there a problem?
FAQ ID -2766
The EpiTect ChIP qPCR primers I used show very high Ct. Are there any solutions?
FAQ ID -2750
The pathway reporter luciferase activity values are less than the negative control, is there a problem?
FAQ ID -2767
How can I prevent the evaporation of reaction volume from the wells EpiTect Methyl qPCR Arrays?
FAQ ID -2742
How can RNA interference be used as a life science research tool?
FAQ ID -2756
How can I be sure that the restriction enzyme digestion is complete in the EpiTect Methyl qPCR system?
FAQ ID -2731
How can I normalize my EpiTect ChIP results?
FAQ ID -2751
What is the recommended amount of input template for each RT² qPCR Primer Assay?
FAQ ID -2716
What is the RT² Profiler PCR Array?
FAQ ID -2718
Can the EpiTect Methyl qPCR System technology reliably characterize heterogenous tissue samples?
FAQ ID -2732
Can users insert custom transcriptional regulatory elements or promoters into the Cignal Reporter Assays?
FAQ ID -2765
Can you custom design the Epitect qPCR primers for the CpG island outside of the defined promoter region?
FAQ ID -2744
Can I manually set the threshold line?
FAQ ID -2702
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