Products
Experiment Configurator
Discovery & Translational Research
DNA & RNA Purification
DNA
Cell-Free DNA
DNA Clean Up
Genomic DNA
Microbial DNA
Plasmid DNA
RNA
Cell-Free RNA
Microbial RNA
miRNA
mRNA
RNA Clean Up
Total RNA
Multianalyte & Virus
Instruments & Equipment
Protein Purification
Fractionation & Depletion
Tagged Protein Expression, Purification, Detection
Exosomes & CTCs
Circulating Tumor Cells
Exosomes
Enzymes for Molecular Biology
Enzymes PCR and DNA amplification
Enzymes RNA analysis
Enzymes assay and detection
Enzymes reaction cleanup and yield improvement
Enzymes cloning and recombinant DNA technology
Enzymes nucleic acid labeling
Sample Collection & Stabilization
DNA
RNA
Tissue/FFPE
QIAcard FTA
PCR/qPCR/dPCR
PCR Enzymes & Kits
End-Point PCR
HiFidelity, Long-Range & Other PCR
One-Step RT-PCR
Reverse Transcription & cDNA Synthesis
Real-Time PCR Enzymes & Kits
Probe-Based One-Step qRT-PCR
Probe-Based qPCR
Reverse Transcription & cDNA Synthesis for qPCR
SYBR Green- or Dye-Based One-Step qRT-PCR
SYBR Green- or Dye-Based qPCR
qPCR Assays & Instruments
CNV qPCR Assays & Arrays
Microbial DNA qPCR Assays & Panels
miRNA qPCR Assay & Panels
mRNA/IncRNA qPCR Assays & Panels
Somatic Mutations qPCR Assays & Panels
Food Safety Testing
Preamplification
Preamplification Kits
REPLI-g
dPCR Assays Kits & Instruments
dPCR Instruments
dPCR Kits
dPCR Assays
dPCR Accessories
Custom LNA Oligonucleotides
Next-Generation Sequencing
DNA Sequencing
Whole Genome Sequencing
Whole Exome Sequencing
Targeted DNA Panels
Single Cell DNA
Custom DNA Panels
Whole Genome
RNA Sequencing
3’ RNAseq
miRNA & Small RNAseq
RNA Fusions
Stranded RNAseq
Targeted RNA Panels
T-Cell Receptor Sequencing
Single Cell RNA
Ultraplex 3’ Targeted
Ribosomal RNA & Globin mRNA Removal
Custom RNA Panels
Multianalyte Sequencing (Multimodal)
Multimodal Panels
Single Cell DNA/RNA
Custom Multimodal Panels
Sample Quality Control
Whole Genome/Transcriptome Amplification
Single Cell & Low Input
Whole Genome
Whole Transcriptome
DNA Methylation Analysis
Metagenomics
Whole Genome Metagenomics
Targeted Metagenomics
Metatranscriptome Analysis
Informatics & Data
Pyrosequencing
ISH & Northern Blotting
Epigenetics
Chromosome Conformation
DNA Methylation
Bisulfite Conversion Assays
Methylation-Specific PCR
Pyrosequencing
DNA Methylation Analysis
Histone Modification
Functional & Cell Analysis
ELISA Assays
Gene Reporter Assays
miRNA Functional Analysis
RNA Silencing
Transfection
CRISPR
Lab Essentials
Buffers & Reagents
Centrifuges
Enzymes
Gel Electrophoresis
Magnets
Plastics
Vacuum Manifolds & Accessories
GeneGlobe Design & Analysis Hub
Diagnostics & Clinical Research
TB Management
QuantiFERON-TB Gold Plus
Transplant
QuantiFERON Transplant
artus Viral Load
Infectious Disease
QIAstat-Dx Syndromic Testing
NeuMoDx Automated Real-Time PCR
Oncology
therascreen Solid Tumor
ipsogen Leukemia
Circulating Tumor Cells
DNA Damage Response
Sexual & Reproductive Health
Cervical Cancer Screening
Maternal/Fetal Testing
STI Testing
Sample Processing
Solutions for Laboratory-Developed Tests
Human ID & Forensics
Forensic Sample Preparation
Sample Collection
Investigator Solutions
Human DNA Quantification
STR Technology
Automation
Next-Generation Sequencing
Pyrosequencing
Validation Services
Next-Generation Sequencing
DNA Sequencing
Whole Genome Sequencing
Whole Exome Sequencing
Targeted DNA Panels
Single Cell DNA
Custom DNA Panels
Whole Genome
RNA Sequencing
3’ RNAseq
miRNA & Small RNAseq
RNA Fusions
Stranded RNAseq
Targeted RNA Panels
T-Cell Receptor Sequencing
Single Cell RNA
Ultraplex 3’ Targeted
Ribosomal RNA & Globin mRNA Removal
Custom RNA Panels
Multianalyte Sequencing (Multimodal)
Multimodal Panels
Single Cell DNA/RNA
Custom Multimodal Panels
Sample Quality Control
Whole Genome/Transcriptome Amplification
Single Cell & Low Input
Whole Genome
Whole Transcriptome
DNA Methylation Analysis
Metagenomics
Whole Genome Metagenomics
Targeted Metagenomics
Metatranscriptome Analysis
Informatics & Data
Instruments & Automation
Nucleic Acid Purification
PCR Instruments
PCR Setup & Liquid Handling
Pyrosequencing
Quality Control & Fragment Analysis
Service Solutions
Accessories
Analytics Software
Automatable Kits
QIAcube Connect Kits
QIAcube HT Kits
QIAsymphony Kits
EZ1 Kits
EZ2 Kits
Tissue Disruption
Informatics & Data
Analysis & Visualization
Interpretation & Content Databases
Enterprise Solutions
Clinical
GeneGlobe Design & Analysis Hub
Services
Genomic Services
Service Solutions
Bioinformatics Services
Strategic partnerships
OEM Enzymes
Proteinase K
Reverse Transcriptase
DNA Polymerase
RNA Polymerase
DNA Ligases
RNA Ligases
NGS Components
PCR Mixes
Modifying Enzymes
Nucleotides
Binding Proteins
OEM PCR Master Mixes
OEM Oligonucleotides
OEM Nucleic Acid Purification
OEM Spin Columns
Top Sellers
New Solutions
Shop
Applications & Insights
Applications & Insights Overview
Research & Technology
Molecular Biology Research
Automated Sample Preparation
PCR
Digital PCR
CRISPR
Next-Generation Sequencing
Liquid Biopsy
Cancer Research
Microbiome
Core Labs
Applied & Industrial
Enzymes for Molecular Biology
Pharma & Biotech
Core Labs
Genomic Services
HID & Forensics
Digital Tools & Platforms
GeneGlobe Design & Analysis
Informatics and Data
QIAsphere Digital Connectivity
Diagnostic & Clinical
Clinical dPCR
Automated PCR
TB Management
Syndromic Testing
Infectious Disease
Oncology
Laboratory Developed Tests
Partnering for Precision Diagnostics
Gynecological & Maternal Health
Plan your experiment
Strategic partnerships
Knowledge & Support
Knowledge & Support Overview
Service Solutions
Service Agreements
QIAlab Consulting
One-Time Services
Professional Services
Events & Webinars
On-Demand Sessions
Upcoming Events & Webinars
Safety Data Sheets
Knowledge Hub
Blogs
Digital Insights
Science Matters
Bench Guide
Cell-free DNA Guide
DNA
RNA
Animal Cell Culture
Epigenetics
Next-Generation Sequencing
PCR
Plasmid
Protein
Transfection
Whole Genome Amplification
Technology & Research
LNA Technology
HRM Technology
Plasmid Resource Center
Pyrosequencing Resource Center
Sample Quality Control
Whole Genome Amplification
Oncology Companion Diagnostics
Explainer Videos & Demos
Events & Webinars
Upcoming Events & Webinars
On-Demand Sessions
Digital PCR Festival 2023
Digital PCR 23
Virtual Seminars On-request
QFT-Plus Webinar Hub
QIAseq Webinar Hub
Medical Education
Pathway Research
Quality, Environmental, Health & Safety
Certificates of Analysis
Quality Assurance and Environmental, Health & Safety
GHS Hazard Labeling
QIAGEN Exclusion Database Service
Product & Technical Support
Troubleshooting guide
Quality, Environmental, Health & Safety
Life Science Grants
New Lab Program
IVDR Support
Green Packaging
Workflow Configurator
QIAsphere Digital Connectivity
Ordering Support
Marketplace Solutions
QIAstock
My QIAGEN
Local Ordering
Terms & Conditions
Resources
Product FAQ
About QIAGEN
About Us
Our Vision
Our Customers
Our Products
Our Global Presence
Our History
Our Views
Our Leadership
Executive Committee
Supervisory Board
Scientific Advisory Board
Careers
Trainee Programs
Student Programs
Why QIAGEN
Meet the QIAGENers
Your Path to QIAGEN
Contact Us
Find jobs
Investor Relations
At a Glance
Strategy
Key Figures
Equity Story
Insights Magazine
Stock Information
Share Data
Share Price
Shareholder Structure
Analyst Coverage
Analyst Consensus
Share Buyback
FAQs
Financial Reports & SEC Filings
Financial Reports
Insights Magazine
Other Reports
Ad-Hoc Announcements
Directors Dealings
Annual General Meeting
Corporate Governance
Governance Documents
Leadership
German Corporate Governance Code
Compliance & Sustainability
Compliance
Sustainability
Events & Presentations
IR Contacts
Stock Transfer Agent
Email Alerts
Information Request Form
IR Contacts
RSS
Newsroom
Press Releases
Resources
Media kit
Video Footage
Photo Archive
Press Contact
Customer stories
Sustainability
Environment
Employees
Society
A letter from the CEO
ESG Ratings
Compliance
Report a Concern
Human Rights
Supply Chain
Contact Us
Order Support
Technical Questions
Human Resources
Business Development
Pharmaceutical Industry
Media Inquiries
Investor Relations Contacts
Global Contacts
QIAGEN Subsidiaries
Commercial Partner and Distributor Solutions
QIAGEN Divisions
My QIAGEN
Cart
Product FAQ
Print
Bookmark
Share
more..
You are not authorized to download the resource
Sort options
Sort alphabetically
Sort by relevance
Why is the Type-it Mutation Detect PCR Kit recommended for preamplification of SNPs?
FAQ ID -2067
View
Why is the storage time for QuantiFast PCR Kits shorter than that for QuantiTect PCR Kits?
FAQ ID -1446
View
Why is the RT step with the QuantiFast RT Kits much shorter compared to QuantiTect RT Kits?
FAQ ID -1451
View
Why is the reaction volume for QuantiFast PCR Kits lower than that for QuantiTect PCR Kits?
FAQ ID -1447
View
Why is the QuantiFast denaturation step different for PCR and RT-PCR runs in the two-step protocol for the ABI 7500 and other cyclers?
FAQ ID -1442
View
Why is the qRT-PCR reproducibility so critical when detecting gene expression knock down in an RNAi experiment?
FAQ ID -2774
View
Why is the QIAcube Connect App reacting so slowly?
FAQ ID - 141519
View
Why is the QIAamp Viral RNA Mini Kit (50) currently unavailable?
FAQ ID -147395
View
Why is the pQE DNA provided in QIAexpress Kits blue in color?
FAQ ID -487
View
Why is the Phoenix Hot Start Taq sometimes cloudy upon removing from -20°C storage?
FAQ ID - 3906
View
Why is the magnesium (Mg2+) solution supplied in a separate tube in the artus® Parvo B19 PCR Kits?
FAQ ID -1533
View
Why is the initial fluorescence signal too high when using artus PCR assays on the Rotor-Gene cycler?
FAQ ID -1497
View
Why is the initial activation step different for Rotor-Gene Probe, SYBR Green and Multiplex Kits?
FAQ ID -2118
View
Why is the IC not detectable although the analytical PCR is positive?
FAQ ID -1505
View
Why is the IC not detectable although the analytical PCR is negative?
FAQ ID -1506
View
Why is the fluorescence signal of the Internal Control on my Rotor-Gene Q very low?
FAQ ID -2574
View
Why is the final heat denaturation step so important with the QIAsymphony PAXgene Blood RNA protocol?
FAQ ID -2987
View
Why is the copy number call lower than expected?
FAQ ID — 3413
View
Why is the activation time for HotStarTaq Plus Polymerase in the QuantiFast SYBR Green Kits different from that for QuantiFast Probe Kits?
FAQ ID -1449
View
Why is special Type-it Fast SNP Probe PCR chemistry required for TaqMan® SNP Genotyping?
FAQ ID -2057
View
Why is my plasmid DNA yield low?
FAQ ID -768
View
Why is my no template control (NTC) real-time Ct value < 35 cycles in my qPCR Assay?
FAQ ID -2686
View
Why is my EpiTect Methyl qPCR Assay "failed" as indicated in the QC page of the data analysis Excel file?
FAQ ID -2734
View
Why is my 260/280 ratio low after using the DNeasy mericon Food Kit?
FAQ ID - 3349
View
Why is maximum amplicon size for the Type-it Microsatellite PCR Kit limited to 500 bp?
FAQ ID -2060
View
Why is it recommended to add 1 mM IPTG for optimal protein yields using the EasyXpress Protein Synthesis Kits?
FAQ ID -860
View
Why is it not recommended to stabilize cells with RNAprotect Tissue Reagent?
FAQ ID -941
View
Why is EvaGreen instead of SYBR Green used as fluorescent dye in the Type-it HRM PCR Kit?
FAQ ID -2196
View
Why is DTT used only for semen samples?
FAQ ID -
143761
View
Why is CoralLoad included in the Type-it Mutation Detect, but not in the Type-it Microsatellite PCR Kit?
FAQ ID -2068
View
Why is carrier RNA used during the isolation of gDNA from microdissected samples with the QIAamp DNA Micro Kit?
FAQ ID -473
View
Why is an ice-incubation step included during reaction set-up when following the QuantiTect RT-PCR but not the QuantiTect PCR protocol.
FAQ ID -283
View
Why is a blood collection set required to collect blood into the PAXgene Blood ccfDNA Tube?
FAQ ID - 3628
View
Why is a blood collection set required to be used with the PAXgene Blood RNA tube?
FAQ ID - 3459
View
Why is a 2-step (and not a 3-step) cycling protocol recommended for Rotor-Gene SYBR Green Kits?
FAQ ID -2122
View
Why is a 2-step (and not a 3-step) cycling protocol recommended for QuantiFast SYBR Green PCR Kits?
FAQ ID -1450
View
Why is 18S ribosomal RNA (rRNA) used as a housekeeping gene to normalize sample-to-sample, systematic variation in qPCR assays?
FAQ ID -2675
View
Why have the hazard symbols accompanying products changed?
FAQ ID - 3430
View
Why had my RT² SYBR Green Mastermix been working well in the past, but now does not seem to be?
FAQ ID -2717
View
Why does the upper aqueous phase look pinkish when purifying RNA from fatty tissue?
FAQ ID -3118
View
Why does the template for EasyXpress Disulfide Insect Kits need to have a signal sequence (such as the mellitin signal sequence as provided by the EasyXpress Linear Template Fab Kit primers)?
FAQ ID -2967
View
Why does the SeqTarget Prep Protocol require a 2-step procedure?
FAQ ID -2238
View
Why does the QuantiTect Primer Assay for my gene of interest have only one version number?
FAQ ID -1134
View
Why does the QIAcube sometimes fail to pick up the tips or pick up wrong tips?
FAQ ID -3131
View
Why does the QIAamp DNA Mini Tissue Protocol require both ATL and AL buffer, while the Blood Protocol only uses AL?
FAQ ID -633
View
Why does the miScript Target Protector not induce the degradation process?
FAQ ID -2263
View
Why does the fluorescence signal drop sharply at the beginning of an artus® PCR run?
FAQ ID -1498
View
Why does the DNeasy mericon Food Kit use a QIAquick column and Buffer PB rather than a DNeasy column and Buffer AL, which might be expected since the kit isolates genomic DNA?
FAQ ID - 3347
View
Why does the DNeasy mericon Food extraction kit use a QIAquick column and not DNeasy column?
FAQ ID -3148
View
Why does the copy function not apply relative to migration time and normalized area values after loading the DNA size marker table and adding the alignment marker peaks on the QIAxcel System?
FAQ ID -1832
View
Why does the connection test fail?
FAQ ID - 141522
View
Why does the Allprep DNA/RNA 96 Kit use Buffer RLT, whereas the AllPrep DNA/RNA Mini Kit uses Buffer RLT Plus?
FAQ ID -1999
View
Why does the A peak reduction factor have to de set to 0.86 for the codon 61 assay in the therascreen NRAS Pyro Kit?
FAQ ID -2390
View
Why does TA/UA cloning work with your proofreading HotStar HiFidelity DNA Polymerase?
FAQ ID -1053
View
Why does my realtime PCR assay quality decrease over time?
FAQ ID -589
View
Why does my purified DNA/RNA sometimes have a 260/280 or 260/230 ratio of more than 2? Does that mean the purity is poor?
FAQ ID -3132
View
Why does my PCR product show up later when comparing the QuantiTect SYBR Green PCR Kits with Roche kits using the same annealing temperature?
FAQ ID -1083
View
Why does my isolated RNA have a low OD 260/280 ratio?
FAQ ID -97
View
Why does my DNA sample float out of the slot when loading it onto an agarose gel?
FAQ ID -205
View
Why does AllPrep DNA/RNA/Protein Mini kit use buffer RLT, but Allprep DNA/RNA Mini and Allprep DNA/RNA Micro kits use buffer RLT Plus? Are these buffers interchangeable?
FAQ ID - 3391
View
Why do you recommend using Triton X for the purification of 6xHis-tagged protein?
-100
View
Why do the Internal Control templates for extraction (Internal Control DNA or RNA [High conc.]) have a 10x higher concentration than the IC templates provided with the QuantiFast Pathogen PCR +IC Kit and the QuantiFast Pathogen RT-PCR +IC Kit?
FAQ ID -2603
View
Why do the amplification curves generated with ABI PRISM® 7000 SDS instrument look rather zigzag-shaped when compared to results generated with the ABI PRISM® 7700 SDS instrument?
FAQ ID -1522
View
Why do replicates in real-time PCR have different plateau heights?
FAQ ID -539
View
Why do QuantiFast SYBR Green PCR Kits require such a high primer concentration?
FAQ ID -1443
View
Why do qBiomarker Copy Number PCR Arrays have assays in quadruplicate?
FAQ ID — 3414
View
Why do pQE-T7 vectors of the QIAgenes E. coli system have an additional UAG amber stop codon?
FAQ ID -2043
View
Why do my qPCR amplification curves or plots decrease in fluorescence intensity after the saturation phase?
FAQ ID -2689
View
Why do melting temperatures differ between PCR fragments amplified with QIAGEN's QuantiTect SYBR Green PCR Kits and Roche Kits?
FAQ ID -1084
View
Why do I sometimes get light blue colonies when using the QIAGEN PCR Cloning Kit?
FAQ ID -603
View
Why do I see multiple high-intensity peaks in my qPCR dissociation curve at temperatures less than 70 ºC?
FAQ ID -2690
View
Why do I see low, poor, or sub-standard amplification efficiency in my qRT-PCR assay?
FAQ ID -2695
View
Why do I observe a CT shift for the Internal Control in some samples in comparison to the CT value for the Internal Control of the dilution series of the Male Control DNA M1?
FAQ ID - 3745
View
Why do I need to identify my real-time instrument model when placing my order for RT² qPCR Primer Assays?
FAQ ID -2713
View
Why do I need normalization using Rotor-Gene ScreenClust HRM Software?
FAQ ID -2198
View
Why do I have wavy DNA bands on my agarose gel?
FAQ ID -2260
View
Why do I have to use an ACS (Assay Control Set)?
FAQ ID -2933
View
Why do I have to add beta-mercaptoethanol (beta-ME) to lysis Buffer RLT of the RNeasy Kits?
FAQ ID -101
View
Why do I get smeared PCR products?
FAQ ID -87
View
Why do I get genomic DNA contamination in my plasmid prep?
FAQ ID -353
View
Why do I get different results for the loci SE33 if I analyze the same sample using the Investigator Nonaplex ESS Kit or Investigator Triplex AFS QS Kit?
FAQ ID -2401
View
Why do I get amplification in a negative control DNA tube using the REPLI-g Kit for WGA?
FAQ ID -675
View
Why do casework-sample protocols for the QIAsymphony DNA Investigator Kit start with different lysate volumes (200 µl, 500 µl, or 1000 µl)?
FAQ ID -2035
View
Why did you choose ORF1a+ORF1b and ORF3a+ORF7a?
FAQ ID - 3893
View
Why did you choose MAPK1 as a positive control in the QIAGEN RNAi Human/Mouse Control Kit?
FAQ ID -604
View
Why did we not choose commonly used genes such as the S gene, E gene, or RdRP?
FAQ ID - 3892
View
Why did the real-time PCR yield Ct values < 12?
FAQ ID -2727
View
Why can’t I see my QIAcube Connect instrument in the QIAcube Connect App?
FAQ ID - 1414518
View
Why can't I find the DNA methylation qPCR Assay for my gene of interest?
FAQ ID -2743
View
Why can I not find the QuantiTect Primer Assay in GeneGlobe that I had previously ordered from QIAGEN?
FAQ ID -1138
View
Why can I not find the Q-Base device in the device list during the first installation?
FAQ ID - 141516
View
Why are two reagents used in the PAXgene Tissue System?
FAQ ID -3022
View
Why are two reagents used in the PAXgene Tissue System?
FAQ ID - 3603
View
Why are there various different QuantiTect Primer Assays for my gene (previous versions, transcript variants)?
FAQ ID -1133
View
Why are there various different QuantiFast Probe Assays for my gene (previous versions, transcript variants)?
FAQ ID -2366
View
Why are the RT² qPCR Primer Assays not designed to cross exon-intron junctions or boundaries?
FAQ ID -2710
View
Why are the QuantiTect and QuantiFast Multiplex PCR Kits limited to triplex real-time PCR on some cyclers?
FAQ ID -715
View
Why are the QuantiFast Probe Assays located on a single exon, thereby also detecting genomic DNA?
FAQ ID -2365
View
Why are the fluorescence intensities of the HSV detection markedly lower than those of the HSV-1 detection using the artus® HSV 1/2 LC PCR Kit?
-2
View
Why are the denaturation and annealing/extension times for QuantiFast Multiplex RT-PCR Kits much shorter than those for QuantiFast Multiplex PCR Kits?
FAQ ID -2147
View
Why are the centrifugation speeds for the QIAamp DNA Mini kit at 6000 x g? Can I spin at full speed?
FAQ ID -474
View
Why are some of my samples outside of the cluster using Rotor-Gene ScreenClust HRM Software?
FAQ ID -2204
View
Why are some of my RNAprotect-stabilized samples frozen at 0ºC while others are not?
FAQ ID -758
View
Why are samples centrifuged at 4°C after the addition of chloroform when using RNeasy Lipid Tissue Kits?
FAQ ID -533
View
Why are samples centrifuged at 4°C after addition of chloroform to the QIAzol lysates in following kits: RNeasy Lipid Tissue Mini/Midi kits, RNeasy Microarray Tissue Mini kit, RNeasy Plus Universal Mini/Midi kit, RNeasy 96 Universal Tissue kit, miRNeasy Mini kit, miRNeasy Micro kit, miRNeasy 96 kit and miRNeasy Serum/Plasma kit?
FAQ ID -2345
View
Why are not all applications/kits/protocol files visible in the run setup (QIAcube Connect App)?
FAQ ID - 1414520
View
Why are my realtime PCR amplification plots hook-shaped?
FAQ ID -587
View
Why are my qPCR Ct values too low (< 12) in my qRT-PCR Assay?
FAQ ID -2684
View
Why are my qPCR Ct values too high (> 35 or not detectable) in my qRT-PCR assay?
FAQ ID -2685
View
Why are my DV200 values of my RNA sample so low?
FAQ ID - 3957
View
Show more results
Back to top
Contact QIAGEN
Global contacts
Technical Service
Customer Care
FAQListPage