Product FAQ - QIAGEN



Product FAQ Bookmark Share more.. You are not authorized to download the resource Sort options Sort alphabetically Sort by relevance 3330 - Is there any contamination from E. coli genomic DNA in the polymerase provided with the Repli-G Single Cell Kit? View 3329 - When using REPLI-g, if the starting material is a single sorted cell, could mitochondrial DNA (mtDNA) be amplified? View 3331 - Should the REPLI-g amplified mtDNA be cleaned up before sequencing? View 3332 - Is there a method that can distinguish between non-amplified DNA and REPLI-g amplified DNA? View 3333 - When using REPLI-g, what is the purpose of the DTT added to the denaturation buffer in the cells/blood protocol? View What sample types can be tested on the arrays/assays? FAQ ID — 3399 View What are the minimum sample requirements for Microbial DNA qPCR kits? FAQ ID — 3393 View Can I use the Microbial DNA-Free Water and Microbial qPCR Mastermix if they have been opened more than 3 times? FAQ ID — 3398 View View 3353 - What is the composition of the elution buffer in the Ni-NTA Fast Start Kit? FAQ ID - 3353 View 3352 - What are the size ranges of Ni-NTA particles? FAQ ID - 3352 View 3354 - What type and amount of resin is packed into the Ni-NTA Spin columns from the Ni-NTA Spin Kit? FAQ ID - 3354 View 3341 - How are the EpiTect DNA controls produced and tested? FAQ - 3341 View 3351 - What is the upper limit for protein size that can bind to Ni-NTA agarose resin? FAQ ID - 3351 View 3344 - What is the difference between the EpiTect Plus DNA Bisulfite kit compared to the classic kit? FAQ - 3344 View 3345 - What is the difference between the high and low concentration EpiTect Bisulfite Conversion protocol? FAQ - 3345 View 3311 - Can QuickLyse miniprep kits be used with QIAVac 24 Plus? View 3313 - What is the endotoxin level for the QIAGEN Plasmid Plus kits? View 3312 - For DirectPrep 96 miniprep, is it possible to use a half plate and save the other half for later prep? View 3314-What is the concentration of RNase A sold separately? View 3316-What is the concentration of the reconstituted QuantiFast Probe Assay? View View 3325 - What are the catalog numbers for the packing materials for the QIAcube? View 3324 - Do you have a protocol for the QIAprep Spin M13 Kit on the QIAcube? View 3335 - Can I use the DNA directly from enzymatic reaction for Epitect Bisulfite treatment with the Epitect Bisulfite kit (cat. no. 59104)? View 3336 - Can I use the linearized DNA for Epitect Bisulfite treatment with the Epitect Bisulfite kit? View 3339 - Does the miRNeasy Serum/Plasma Spike-in Control (cat n° 219610) negatively impact subsequent NGS sequencing? View 3337 - Which method is used for QIAGEN sequencing service? View 3338 - Does the miRNeasy Serum/Plasma Spike-in Control (cat n° 219610) have 3'-hydroxyl and 5'-phosphate groups? View 3343 - Why is it difficult to quantify DNA using UV/Vis spectroscopy and fluorescence methods following EpiTect Bisulfite Conversion? How best do you quantify it? FAQ - 3343 View 3342 - How is the unmethylated unconverted EpiTect DNA control to be used? FAQ - 3342 View 3315-What is the concentration of the reconstituted QuantiTect Primer Assay (200)? View 3322 - What is the concentration of the reconstituted miScript Primer Assay (100) and miScript Precursor Assay (100) tubes? View 3317 - Do you have a protocol for isolation of genomic DNA from cell cultures using the QIAamp cador Pathogen Mini Kit? View 3318 - What guidelines should I follow in designing primers for use with the EpiTect MSP Kit? View 3320 - What is the sensitivity of the Type-it HRM PCR Kit? View 3321 - What is the purpose of the C. elegans miR-39 spike-in control in the miRNeasy Serum/Plasma kit? View 3318 - What is the preferred amplicon size for performing PCR on DNA amplified with the EpiTect Whole Bisulfitome Kit? View 3305 - What is the minimal amount of starting material required for using the SBTexcellerator HLA Kits? View View View View 3306 - What is the optimal sample DNA concentration for the Investigator ESSplex SE Plus Kit? View 3304 - What is the maximum DNA input volume that can be used in the Investigator ESSplex SE Plus Kit? View 3310 - Which settings for the filter set Any5Dye must be adjusted before running the spectral calibration for Investigator ESSplex SE Plus Kit? View 3308 - Where can I find the template files for the Investigator ESSplex SE Plus Kit? View 3309 - Which filter set should be used with Investigator ESSplex SE Plus Kit? View What additional equipment is needed to purify RNA using the PAXgene 96 Blood RNA Kit? FAQ ID -499 View 3300 - Can isopropanol or methanol be used in place of ethanol in RNeasy kits? View 3301 - Do you have any information or guidelines regarding the choice of reference genes for real-time PCR? View 3302 - How long can I store my purified RNA? View 3210 - Can the 4-16K Centrifuge be used with a 208 V outlet? View 3211 - What is the maximum speed of the plate rotor 2 x 96 in the 4-16 or 4-16K? View 3212 - What is the minimum size of DNA fragment that can be isolated with QIAamp DNA Mini kit? View 3299-Can Buffer ER from the Endofree Plasmid kits be used instead of the Buffer ETR in QIAGEN Plasmid Plus kits? View 3213 - How much ethanol should be added to Buffer AP3/E (renamed to Buffer AW1) and Buffer AW (renamed to Buffer AW2) in the DNeasy Plant kit? View 3202 - Can GeneRead rRNA depletion kit be used to deplete rRNA from miRNA isolated using miRNeasy kit? View 3200 - What is the Maximum/Minimum amount of RNA that can be used with GeneRead rRNA depletion kit? View 3201 - What quality of RNA can be used with the GeneRead rRNA depletion kit? View 3204 - How strict is the purification cut-off with GeneRead Size Selection kit? View 3203 - Is the RNeasy MinElute cleanup kit required for the GeneRead rRNA depletion kit? View 3205 - Can GeneRead Library preparation kits be used on species other than human mouse and rat, for example, plant species and microbial species? View 3206 - Can our GeneRead Library preparation kits be used with the SoLiD Platform? View 3248 - What is the difference between sponge, cubic and lamellar phase? View 3254 - When using the Nextal Cubic Phase products how do I know in which phase the sample is? What will I see? View 3255 - When using the NexTal Cubic Phase products, should I work with diluted or undiluted screens? View 3227 - In which solution can I keep my protein with the NexTal Cubic Phase kits? View 3225 - How pure should my protein be for the NexTal CubicPhase kits? View 3217 - How long can I store my purified RNA? View 3292-How do I start using the NeXtal CubicPhase kits? View 3295-What is the difference between SBTexcellerator HLA Core, Extended and Full Kits? View 3240 - What are the main differences between the qBiomarker PCR Arrays and the RT2 Profiler PCR Arrays? View 3237 - I would like to order the QuantiFast Probe Assays without master mix. Is this possible? View 3233 - Is the yield and quality of RNA and DNA purified with the AllPrep DNA/RNA FFPE Kit comparable to yield and quality of nucleic acids purified separately with other methods. View 3265 - Why are there various different QuantiFast Probe Assays for my gene (previous versions, transcript variants)? View 3298-Will the sequence of the QuantiFast Probe Assays be provided? View 3262 - Which thermal cyclers are validated for use with the Investigator Nonaplex ESS Kit? View 3263 - Why are samples centrifuged at 4°C after the addition of chloroform when using the RNeasy Plus Universal Mini Kit? View 3241 - What are the recommended storage conditions of the QuantiFast Probe RT-PCR Plus Kit components? View 3264 - Why are the QuantiFast Probe Assays located on a single exon, thereby also detecting genomic DNA? View 3238 - What are important points regarding the genomic DNA wipeout buffer in the QuantiFast Probe RT-PCR Plus Kit? View 3245 - What is the concentration of the primers and the probe in a reaction using the QuantiFast Probe Assays? View 3239 - What are the differences between the existing QuantiFast Probe RT-PCR Kit and the new Plus Kit? View 3247 - What is the correct matrix to use with the Investigator Nonaplex ESS Kit? View 3243 - What does the Investigator Nonaplex ESS Kit contain? View 3244 -What is the average length of a QuantiFast Probe Assay? View 3231 - Is there a size limit to the protein I can crystallize with the NexTal Cubic Phase products? View 3228 - Is it also possible to isolate miRNA with the AllPrep DNA/RNA FFPE Kit? View 3232 - Is the RNeasy Plus Universal Mini Kit suited for adipose, brain, and other fatty tissues? View 3229 - Is it necessary to perform calibration steps for the use of the MAX dye in duplex RT-PCR experiments on different cyclers? View 3236 - I use cultured cells as starting material. Can I also use the RNeasy Plus Universal Kit? Show details Is there also a miRNeasy Plus Universal Kit available? View 3261 - Which purification kits are recommended for the therascreen Pyro kits? View 3258 - Which filter set and settings should be used with the Investigator Nonaplex ESS Kit? View 3260 - Which purification kits are recommended for the Pyro kits? View 3259 - Which probe labels are available for the QuantiFast Probe Assays? View 3296-Why does the A peak reduction factor have to de set to 0.86 for the codon 61 assay in the therascreen NRAS Pyro Kit? View What plug-ins are available for the therascreen Pyro kits? FAQ ID - 3251 View 3249 - What is the optimal DNA volume and concentration for the Investigator Nonaplex ESS Kit? View What plug-ins are available for the Pyrosequencing kits? FAQ ID - 3250 View 3252 - What should be the concentration of my protein with the NexTal Cubic Phase products? View 3256 - Which crystallization solutions are compatible with lipidic cubic phase crystallization? View 3253 - What species are QuantiTect Probe Assays offered for? View 3257 - Which deparaffinization method can I use with the AllPrep DNA/RNA FFPE Kit? View Can the insertions in Exon 20 be detected with the therascreen EGFR Pyro Kit? View Can the insertions in Exon 20 be detected with the EGFR Pyro Kit? FAQ ID - 3279 View 3277 - Can I use the RNeasy Plus Universal Mini Kit for all types of tissue? View Can QuantiFast Probe Assays be ordered offline via e-mail or fax? FAQ ID - 3278 View 3283-Do you have any information or guidelines regarding the choice of reference genes for real-time PCR? View 3285-How are the genes in each iPSC qBiomarker PCR Array selected? View 3284-For which applications can the RNA isolated with the RNeasy Plus Universal Kit also be used? View Can the QuantiFast Probe Assay detect short fragmented RNA as well as longer fragments of RNA? View Do the RNeasy Plus Universal Mini/Midi kits contain both wash buffers, RW1 and RWT? FAQ ID - 3282 View 3270 - Are the primer sets different for the Investigator Nonaplex ESS Kit and ESSplex SE where the loci are the same? View Are the QuantiFast Probe Assays hydrolysis or FRET probes? FAQ ID - 3271 View Are panels and bin sets available for use with GeneMapper ID and GeneMapper ID-X? FAQ ID - 3267 View 3275 - Can I use more than 10ng DNA for the PCR reaction for my therascreen Pyro Kits? View Can I use more than 10ng DNA for the PCR reaction for the Pyro kits? FAQ ID - 3276 View 3274 - Can I do singleplex PCR with the QuantiFast Probe Assays? View 3273 - At what temperature can I perform the experiment with the NexTal Cubic Phase kits? View 3272 - Are the QuantiFast Probe Assays wet-lab validated? View 3222 - How much DNA/RNA can be isolated with the AllPrep DNA/RNA FFPE Kit? View 3218 - How many fragments are contained within the size standard BTO in the Investigator Nonaplex ESS Kit? View 3219 - How many freeze-thaw cycles can the master mix contained in the QuantiFast Probe RT-PCR Plus Kit tolerate? View 3220 - How many PCR cycles should I use for the Investigator Nonaplex ESS Kit? View 3226 - How pure should the chloroform used with the RNeasy Plus Universal Mini Kit be? View 3223 - How much sample material can I use for DNA/RNA purification with the AllPrep DNA/RNA FFPE Kit? View 3224 - How much time can be saved when using the RNeasy Mini QIAcube Kit? View 3289-How does the QuantiFast Probe RT-PCR Plus Kit eliminate genomic DNA contamination? View 3290-How do I analyze the data for the particular iPSC qBiomarker Arrays I have ordered? View 3288-How does the gDNA eliminator solution of the RNeasy Plus Universal Kit work? View 3294-How long can I store my purified DNA/RNA isolated with the AllPrep DNA/RNA FFPE kit? View 3286-How can I adjust the ROX concentration in the QuantiFast Probe RT-PCR Plus master mix? View 3291-How do I harvest the crystals from meso phase? Are special tools needed with the NexTal Cubic Phase products? View 3246 - What is the correct matrix to use with Investigator ESSplex SE Plus Kit? View 3297-Why does the PCR for HLA-DRB4 result in 2 amplicons of different size? View 3266 - Why are two different exon 2 forward sequencing primers used for the SBTexcellerator HLA-DRB1 Kit? View How can I find out if my gene of interest is expressed in a specific tissue type or cell line? FAQ ID - 3214 View 3215 - What is the starting material for SBTexcellerator HLA Kits? View Can I use genomic DNA from fixed samples with qBiomarker Copy Number PCR Arrays? FAQ ID — 3416 View Can I use amplified genomic DNA with qBiomarker Copy Number PCR Arrays? FAQ ID — 3415 View Do the samples require pretreatment before starting the manual protocol or before loading onto the QIAcube instrument? FAQ ID - 3479 View What are the expected yields of RNA from PAXgene Blood RNA Tubes isolated with the PAXgene Blood miRNA kit? FAQ ID - 3481 View Do you have stability data for RNA isolated with PAXgene miRNA Kit? FAQ ID - 3480 View Can the PAXgene Blood DNA Tubes be stored at 4°C? FAQ ID - 3493 View How should I thaw frozen PAXgene Blood DNA Tubes? FAQ ID - 3495 View Can the PAXgene Blood DNA Tube be frozen? If yes, at what temperatures and for how long? FAQ ID - 3491 View Can mitochondrial DNA be isolated with the PAXgene Blood DNA System? FAQ ID - 3498 View 3497 - What is the expected purity and size of DNA extracted using the PAXgene Blood DNA kit? FAQ ID - 3497 View Does the DNA extracted using the PAXgene Blood DNA System contain RNA? FAQ ID - 3499 View Can I multiplex the Microbial DNA qPCR Assays? FAQ ID — 3408 View Can I measure virulence factor gene expression? FAQ ID — 3405 View What sample types can I test on qBiomarker Copy Number PCR Arrays? FAQ ID — 3423 View What is the sensitivity for the Microbial DNA qPCR kits? FAQ ID — 3409 View Which probe labels are available for the Microbial DNA qPCR Assays? FAQ ID — 3407 View Are the Microbial DNA qPCR Assays wet-lab verified? FAQ ID — 3406 View Can I measure antibiotic resistance gene expression? FAQ ID — 3404 View What sequences are used to design the Microbial DNA qPCR Assays? FAQ ID — 3403 View Are the assays species-specific? FAQ ID — 3400 View Why is the copy number call lower than expected? FAQ ID — 3413 View Will qBiomarker Copy Number Assays work with heterogeneous samples like mixtures of tumor and normal tissue? FAQ ID — 3419 View Why do qBiomarker Copy Number PCR Arrays have assays in quadruplicate? FAQ ID — 3414 View How should I analyze the data generated from a qBiomarker Copy Number PCR Array experiment? FAQ ID — 3421 View What could have gone wrong if the CT values are unusually high for all wells in a sample? FAQ ID — 3412 View What qPCR mastermix should I use with the qBiomarker Copy Number PCR Arrays and Assays FAQ ID — 3422 View How much DNA should I use on a qBiomarker Copy Number PCR Array? FAQ ID — 3417 View How much DNA should I use in a qBiomarker Copy Number PCR Assay? FAQ ID — 3418 View How do you choose which assays to include on the arrays? FAQ ID — 3420 View What testing should be performed in order to assess the quality of a DNA sample? FAQ ID — 3424 View How many samples can be processed with the PAXgene Blood miRNA Kit? FAQ ID - 3477 View Why have the hazard symbols accompanying products changed? FAQ ID - 3430 View Why is a blood collection set required to be used with the PAXgene Blood RNA tube? FAQ ID - 3459 View As part of my RT procedure I routinely heat an aliquot of the eluate prior to the RT reaction. Do I still need to heat the whole eluate after the RNA preparation? FAQ ID - 3468 View Is there any chance of the PAXgene Blood RNA tube reagent going back into the patient's arm? FAQ ID - 3460 View I need to buy a new computer for my PyroMark Q96. Can I reinstall the PyroMark Q96 software on my new computer or do I need to purchase a new license? FAQ ID - 3472 View Can I reinstall the PyroMark Q24 software on my new computer or following an operating system upgrade, or do I need to purchase a new license? FAQ ID - 3473 View Can the PAXgene Blood DNA Tubes be stored at higher temperatures? FAQ ID - 3494 View What are the expected yields of RNA from PAXgene Blood RNA Tubes using the QIAsymphony PAXgene Blood RNA Kit? FAQ ID - 3475 View What additional equipment is needed to purify RNA using the PAXgene 96 Blood RNA Kit? FAQ ID - 3474 View Is it possible to isolate genomic DNA or viral nucleic acids with the QIAsymphony PAXgene Blood RNA protocol? FAQ ID - 3476 View 2. Are there any special considerations for the collection of blood using PAXgene Blood DNA Tubes CE (e.g., use of a blood collection set or discard tube, positioning of the tube, issues with short draws, etc.)? FAQ ID - 3485 View Are the PAXgene Blood DNA Tubes sterile? FAQ ID - 3487 View What is the additive in PAXgene Blood DNA Tubes FAQ ID - 3486 View What is the maximum number of samples for the QIAcube variant of the PAXgene Blood miRNA protocol for a single run? FAQ ID - 3482 View Do I have to use a blood collection set with the PAXgene Blood DNA Tube? FAQ ID - 3484 View Is it possible to isolate genomic DNA or viral nucleic acids with PAXgene Blood miRNA protocol? FAQ ID - 3483 View How should blood samples drawn into PAXgene Blood DNA Tubes be shipped? FAQ ID - 3489 View How long can the PAXgene Blood DNA Tube be kept at room temperature? What is room temperature? FAQ ID - 3492 View Which sample types can be processed using the PAXgene Blood miRNA protocols? FAQ ID - 3478 View What is the shelf life of the PAXgene Blood DNA Tube prior to blood collection? FAQ ID - 3488 View How should blood samples drawn into the PAXgene Blood DNA Tubes be stored? FAQ ID - 3490 View Can the miScript II RT Kit be used to quantify piRNAs? FAQ ID - 3432 View Can the miScript Plant RT Kit be used to quantify piRNA expression? FAQ ID - 3434 View Can the miScript Plant RT Kit be used to quantify animal miRNAs? FAQ ID - 3433 View Does the miScript Plant RT Kit contain controls? FAQ ID - 3435 View What is the composition of the 5x miScript Plant RT Buffer? FAQ ID - 3437 View Are any of the miScript II RT Kit components interchangeable with the miScript Plant RT Kit components? FAQ ID - 3436 View What should I use to isolate total RNA from plant tissues and what is the maximum recommended amount of tissue input? FAQ ID - 3438 View Are miScript Primer Assays available for plant mature miRNAs? FAQ ID - 3439 View Are plant miScript Primer Assay designs wet-bench validated? FAQ ID - 3440 View Show more results Back to top Contact QIAGEN Global contacts Technical Service Customer Care