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Do you have a protocol for purification of total DNA from crude lysates?
FAQ ID -1255
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Do you have a protocol for isolation of genomic DNA from saliva and mouthwash?
FAQ ID -917
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Do you have a protocol for purification of total DNA from yeast?
FAQ ID -1253
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Do you have a protocol for the isolation of DNA from soft tissues using the TissueLyser?
FAQ ID -924
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Do you have a protocol for purification of total DNA from insects?
FAQ ID -1254
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What is the shelf-life for QIAGEN Proteinase K (cat. no. 19131, 19133)?
FAQ ID - 3447
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Do you have a protocol for the isolation of genomic DNA from sperm?
FAQ ID -909
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What is the advantage of running an analytical gel with fractions of my plasmid preparation?
FAQ ID -769
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What is the recipe for LB?
FAQ ID -212
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How do I know if my plasmid is a high- or low copy number type?
FAQ ID -350
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Can I eliminate RNase A from buffer P1 for my plasmid preparation to obtain RNase-free DNA for in-vitro transcription?
FAQ ID -366
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I left Buffer P1 at room temperature after addition of RNase A, what shall I do?
FAQ ID -859
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With LyseBlue reagent for lysis control, can I now process more bacterial culture and overload the columns?
FAQ ID -864
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What is the recipe for 2x YT?
FAQ ID -213
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What are the additional plasmid bands I see on my gel?
FAQ ID -1059
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Do you have a protocol for the isolation of plasmid DNA from Bacillus subtilis?
FAQ ID -896
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What is the composition of Buffer PB?
FAQ ID -2791
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Do you have a protocol for obtaining > 20 µg plasmid DNA using the QIAprep Spin Miniprep Kit?
FAQ ID -3102
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Why is my plasmid DNA yield low?
FAQ ID -768
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Why do I get genomic DNA contamination in my plasmid prep?
FAQ ID -353
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Do you have a protocol for the isolation of plasmid DNA from Agrobacterium?
FAQ ID -898
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I am seeing a precipitate after adding LyseBlue reagent to Buffer P1. What should I do about that?
FAQ ID -1045
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What to do if cell clumps are present after Buffer P2 addition when using LyseBlue Reagent?
FAQ ID -861
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What is the recipe for SOC medium?
FAQ ID -798
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How much RNA does a typical mammalian cell contain?
FAQ ID -2946
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Do I have to discard Buffer RLT with beta-Mercaptoethanol (ß-ME) added to it after 1 month of storage?
FAQ ID -1037
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What are the effects of low A260/A230 ratios in RNA preparations on downstream applications?
FAQ ID -2248
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Do I need to purchase any consumable for processing the kit on EZ2 Connect?
FAQ ID - 3849
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Are we able to customize protocol on the EZ2 Connect?
FAQ ID - 3850
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Which version of the EZ2 Connect can run the EZ2 RNA/miRNA Tissue/Cells Kit?
FAQ ID - 3851
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Can the kit be run on EZ1 instruments?
FAQ ID - 3852
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How can I check the integrity of RNA purified using RNeasy Kits?
FAQ ID -1024
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What are the limits of detection of the QIAxcel Connect?
FAQ ID - 3853
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Are all QIAxcel Cartridge Kits compatible with QIAxcel Connect?
FAQ ID - 3856
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What are the differences between QIAxcel Advanced and QIAxcel Connect?
FAQ ID - 3857
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What is the QIAxcel Connect System?
FAQ ID - 3859
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Can I combine your CGT dPCR assays with custom designed assays?
FAQ ID - 3861
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How many assays can be multiplexed?
FAQ ID - 3862
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In which channels can you detect the CGT dPCR assays?
FAQ ID - 3863
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How can I resuspend the lyophilized assay?
FAQ ID - 3864
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What quenchers are used?
FAQ ID - 3865
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Do I need to use restriction enzymes when quantifying AAV genomes?
FAQ ID - 3866
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Can the CGT dPCR assays also be used for the quantification of non-AAV samples?
FAQ ID - 3868
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Can I use the CGT dPCR assays for the titer determination of RNA viruses?
FAQ ID - 3869
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How do I know if the assays are compatible with the sample DNA of interest?
FAQ ID - 3870
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Are the assays also compatible with qPCR?
FAQ ID - 3871
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Can they be used with lentiviral vectors?
FAQ ID - 3872
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Is restriction enzyme digestion required?
FAQ ID - 3875
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dPCR results are in copies per microliter. How do I calculate host cell DNA contamination in femtogram per microliter?
FAQ ID - 3876
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My samples are highly fragmented. Is the dPCR result for resDNA quantification accurate?
FAQ ID - 3877
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Is dPCR sensitive to inhibition?
FAQ ID - 3880
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Is DNA extraction from the samples needed?
FAQ ID - 3873
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How much sample can I load for detecting host cell contamination?
FAQ ID - 3879
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Where can I find a copy of the .kpf file for a BioSprint protocol?
FAQ ID -3073
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Can the DyeEx 96 kit be used to clean sequencing reactions to be run on ABI 3730?
FAQ ID -3075
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What is the minimum number of samples that can be extracted on a BioSprint 96?
FAQ ID -3076
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Can the FastLane Cell lysates (containing stabilized RNA, step 8. in Fastlane protocol) be stored?
FAQ ID -3077
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Can I use specific primers with the FastLane Kits?
FAQ ID -3078
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Can the FastLane RT reaction be incubated longer than 30 min at 42 deg C?
FAQ ID -3079
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Does carrier RNA interfere with the QuantiTect Whole Transcriptome amplification?
FAQ ID -3080
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Which SARS-CoV-2 targets can the SARS-CoV-2 Neo Kit detect?
FAQ ID - 3881
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Do we have specificity and sensitivity data for this kit?
FAQ ID - 3882
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Do we have specificity and sensitivity data for this kit?
FAQ ID - 3883
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Is it possible to scale up sample volume and reaction volume?
FAQ ID - 3884
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What color channels are used?
FAQ ID - 3885
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What is the percentage of false negative results that QIAGEN obtained during validations?
FAQ ID - 3886
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What is the minimum and maximum concentration of effective RNA for the test?
FAQ ID - 3887
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Anything important when starting the cycler run?
FAQ ID - 3888
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Is it possible to use ABI instruments without ROX as a passive reference?
FAQ ID - 3889
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What is the shelf-life of the Neo Assay Kit?
FAQ ID - 3890
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Can the SARS-CoV-2 Neo Assay Kit detect all variants?
FAQ ID - 3891
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Why did we not choose commonly used genes such as the S gene, E gene, or RdRP?
FAQ ID - 3892
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Why did you choose ORF1a+ORF1b and ORF3a+ORF7a?
FAQ ID - 3893
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Can I use ROX as passive reference dye with the SARS-CoV-2 Neo assay?
FAQ ID - 3894
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Can I use the IC RNA of the QIAprep& Viral RNA UM Kit?
FAQ ID - 3895
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Which positive control should I use?
FAQ ID - 3896
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How long can eluates be stored after sample preparation using the EZ1 instruments or EZ2 Connect MDx and the EZ1 DSP Virus Kit?
FAQ ID - 3897
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Which materials can be used as input samples for the QIAsymphony DSP Circulating DNA Kit?
FAQ ID - 3702
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Are there important considerations for plasma generation and urine handling?
FAQ ID - 3699
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How stable is Phoenix Hot Start Taq when incubated in a PCR reaction mix at room temperature?
FAQ ID - 3898
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How can PCR cycling conditions be optimized for Phoenix Hot Start Taq?
FAQ ID - 3899
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Can Phoenix Hot Start Taq utilize cDNA as template for PCR?
FAQ ID - 3900
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Is Phoenix Hot Start Taq capable of multiplex PCR?
FAQ ID - 3901
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How can I optimize Mg2+ conditions for a specific amplicon when using Phoenix Hot Start Taq and the supplied reaction buffer?
FAQ ID - 3902
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When should I use Phoenix Hot Start Taq GC reaction Buffer?
FAQ ID - 3903
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What is the amplification length limit of Phoenix Hot Start Taq?
FAQ ID - 3904
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What is the fidelity/error rate of Phoenix Hot Start Taq?
FAQ ID - 3905
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Why is the Phoenix Hot Start Taq sometimes cloudy upon removing from -20°C storage?
FAQ ID - 3906
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How can yield for long targets be increased when using Phoenix Hot Start Taq?
FAQ ID - 3907
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How is VeraSeq 2.0 DNA Polymerase different from the standard recombinant Taq-B DNA Polymerase?
FAQ ID - 3908
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How can I optimize Mg2+ conditions for a specific amplicon when using VeraSeq 2.0 DNA Polymerase and the supplied reaction buffers?
FAQ ID - 3909
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When should I use 5X VeraSeq GC Buffer?
FAQ ID - 3910
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What is the amplification length limit of VeraSeq 2.0 DNA Polymerase?
FAQ ID - 3911
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Why is VeraSeq 2.0 DNA Polymerase sometimes cloudy upon removing from -20°C storage?
FAQ ID - 3913
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What is the amplification length limit of VeraSeq 2.0 DNA Polymerase?
FAQ ID - 3912
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How can yield for targets be increased when using VeraSeq 2.0 DNA Polymerase?
FAQ ID - 3914
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Will VeraSeq 2.0 DNA Polymerase incorporate dUTP?
FAQ ID - 3915
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Is VeraSeq 2.0 DNA Polymerase available as a hot start enzyme?
FAQ ID - 3916
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How long can reaction components incubate with VeraSeq 2.0 DNA Polymerase at room temperature prior to PCR cycling?
FAQ ID - 3917
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What denaturation temperature should be used in cycling conditions?
FAQ ID - 3918
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What annealing temperature should be used in the cycling conditions?
FAQ ID - 3919
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What is the stability of VeraSeq 2.0 DNA Polymerase at room temperature?
FAQ ID - 3920
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How can I optimize Mg2+ conditions for a specific amplicon when using VeraSeq ULtra DNA Polymerase and the supplied reaction buffers?
FAQ ID - 3922
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How is VeraSeq ULtra DNA Polymerase different from the standard recombinant Taq-B DNA Polymerase?
FAQ ID - 3921
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When should I use 5X VeraSeq GC Buffer?
FAQ ID - 3923
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What is the amplification length limit of VeraSeq ULtra DNA Polymerase?
FAQ ID - 3924
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What is the fidelity/error rate of VeraSeq ULtra DNA Polymerase?
FAQ ID - 3925
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How can yield for targets be increased when using VeraSeq ULtra DNA Polymerase?
FAQ ID - 3926
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Will VeraSeq ULtra DNA Polymerase incorporate dUTP?
FAQ ID - 3927
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Do the DNA fragments generated by VeraSeq ULtra DNA Polymerase have a single-base 3´ overhang?
FAQ ID - 3928
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Is VeraSeq ULtra DNA Polymerase available as a hot start enzyme?
FAQ ID - 3929
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What denaturation temperature should be used in the cycling conditions?
FAQ ID - 3931
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What annealing temperature should be used in the cycling conditions?
FAQ ID - 3932
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What are the ResDNA Quant Kit components?
FAQ ID - 3878
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How can I load new protocols onto the QIAcube?
FAQ ID -1421
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Are run reports and/or log files available on the QIAcube?
FAQ ID -1412
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What applications are offered on the QIAcube?
FAQ ID -1403
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Can the QIAcube heater/shaker be used independently from protocol runs?
FAQ ID -1422
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What EZ1 DNA Investigator protocol is recommended for very precious samples?
FAQ ID -1154
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When should the Normalization protocol be used?
FAQ ID -1155
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What fluorescent labels are 6-FAM, BTG, BTY, BTR, and BTO in Matrix Standard BT5 single or multi.cap?
FAQ ID - 3364
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Does QIAGEN provide data analysis to accompany the EpiTect Hi-C Kit?
FAQ ID -
143075
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How can I extract DNA from a polyacrylamide (PAGE) gel?
FAQ ID -120
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Why does my DNA sample float out of the slot when loading it onto an agarose gel?
FAQ ID -205
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What is the small band below my fragment of interest on an agarose gel after DNA cleanup using QIAquick?
FAQ ID -148
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I bound an 11 kb DNA fragment to a QIAquick column; is it completely lost?
FAQ ID -756
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Is it possible to isolate single stranded DNA (ssDNA) with the QIAEX II Kit from agarose or polyacrylamide gels?
FAQ ID -576
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Can DNA yields be increased by prolonging incubation with proteinase K in Buffer ATL?
FAQ ID -2032
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Can the QIAcube be connected to a laboratory information management system?
FAQ ID -1411
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What are the dimensions and weight of the QIAcube?
FAQ ID -1407
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What dedicated QIAcube Kits are available?
FAQ ID -2337
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How can I decontaminate the QIAcube system?
FAQ ID -1419
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Can the QIAcube rotor and/or buckets be removed for cleaning?
FAQ ID -1410
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Can the QIAcube centrifuge be used independently from protocol runs?
FAQ ID -1423
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Do I need to buy special kits for the QIAcube?
FAQ ID -1402
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Do I need to discard partially used QIAcube tip racks?
FAQ ID -1418
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Can I program my own protocols for the QIAcube?
FAQ ID -1405
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Can the CGT dPCR assays also be used for the analysis of AAV genome integrity?
FAQ ID - 3867
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Is the QIAprep& CRISPR Kit also applicable to other gene-editing technologies such as TALENs and ZFN?
3787
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Do you have a protocol for the isolation of viral RNA from stool?
FAQ ID -918
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Which extraction kits are recommended?
FAQ ID - 3874
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How long can I store the reconsituted assay regarding QIAcuity HEK293 resDNA Quant Kit (96) at 4°C?
FAQ ID - 3934
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What is the preferred storage temperature for reconstituted assay for the QIAcuity HEK293 resDNA Quant Kit (96)?
FAQ ID - 3935
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What is the amount of standard for the different kit?
FAQ ID - 3936
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How did you calculate the conversion factor?
FAQ ID - 3937
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What does the standard kit contain?
FAQ ID - 3938
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What region is targeted in the QIAcuity E. coli resDNA Quant Kit (96)?
FAQ ID - 3939
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How long are sample lysates stable in the fridge/ freezer?
FAQ ID -
143765
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Can solid samples be processed with the QIAsymphony DNA Investigator Kit on the instrument?
FAQ ID -2027
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Should sample lysate volumes be exactly 200 µL, 500 µL, or 1000 µL when loaded on the QIAsymphony SP?
FAQ ID -2031
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Which version of the EZ2 Connect can run the EZ2 RNA FFPE Kit?
FAQ ID - 3940
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When should I use the standard vs. the fast protocol for the EZ2 RNA FFPE Kit?
FAQ ID - 3941
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What size of RNA can be expected?
FAQ ID - 3942
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Which version of the EZ2 Connect can run the EZ2 AllPrep DNA/RNA FFPE Kit?
FAQ ID - 3947
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When should I use the ‘Standard’ vs. the ‘Fast’ protocol for the EZ2 AllPrep DNA/RNA Kit
FAQ ID - 3948
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Are there other options for paraffin removal?
FAQ ID - 3949
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What are recommended stopping points in the procedure of the EZ2 AllPrep DNA/RNA FFPE Kit?
FAQ ID - 3950
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Why is there a need to overlay the resuspended DNA pellet with PRS again (step 17 of QSP)?
FAQ ID - 3951
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An immediate subsequent AllPrep DNA/RNA FFPE run does not start right away.
FAQ ID - 3952
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Can I use the EZ2 AllPrep DNA/RNA FFPE kit to extract DNA/RNA from fresh frozen tissue?
FAQ ID - 3953
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What size of RNA can be expected?
FAQ ID - 3954
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Are there specific recommendations for performing RT-PCR on RNA isolated from paraffin-embedded samples?
FAQ ID - 3955
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My overall RNA yield is fine but RT-PCR performance poor – how can I improve this?
FAQ ID - 3956
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Why are my DV200 values of my RNA sample so low?
FAQ ID - 3957
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The integrity of my DNA sample (e.g. QIAxcel Connect) is bad.
FAQ ID - 3958
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How do I proceed if the amount of starting material cannot be calculated in detail?
FAQ ID - 3943
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Are there other options for paraffin removal?
FAQ ID - 3946
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Are there specific recommendations for performing RT-PCR on RNA isolated from paraffin-embedded samples?
FAQ ID - 3944
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My overall RNA yield is fine but RT-PCR performance poor – how can I improve this?
FAQ ID - 3945
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Is this available without glycerol?
FAQ ID - 3959
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What types of priming are compatible with EnzScript M-MLV RT RNase H-?
FAQ ID - 3961
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What is the suggested protocol for generating long, full-length cDNA transcripts (> 5 kB)?
FAQ ID - 3964
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What are the advantages of EnzScript M-MLV Reverse Transcriptase RNase H- (P7600) versus wild type M-MLV Reverse Transcriptase (P7040)?
FAQ ID - 3960
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What PCR enzymes can be used following first-strand synthesis?
FAQ ID - 3963
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Is the kit compatible with unpurified in-process samples?
FAQ ID - 3965
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Do I need to use Proteinase K in the CGT Viral Vector Lysis Kit? What is the recommended concentration?
FAQ ID - 3971
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Do you recommend storage of highly diluted AAV samples?
FAQ ID - 3972
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Is it possible to store the lysates? How can the lysates be stored?
FAQ ID - 3973
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Can I use MspI or HpaII restriction enzymes from other suppliers?
FAQ ID - 3975
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How many AAV genome copies can I load into the QIAcuity?
FAQ ID - 3977
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Do you need to dilute the lysates before setting up the PCR mix?
FAQ ID - 3978
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How do I know if the CGT dPCR assays are compatible with the sample DNA of interest?
FAQ ID - 3980
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Are the CGT dPCR assays also compatible with qPCR?
FAQ ID - 3981
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Can the CGT Viral Vector Lysis kit be used with assays of other suppliers or custom designed assays?
FAQ ID - 3982
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What is included in the SureSilencing shRNA Plasmids shipment?
FAQ ID -2785
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Is the QIAxcel Advanced discontinued?
FAQ ID - 3983
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What is the concentration range for RNA Integrity Score analysis when using the QIAxcel RNA High Sensitivity Kit?
FAQ ID - 3984
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What effect does homogenization have on DNA yield and integrity when using QIAwave DNA/RNA Kits?
FAQ ID - 3985
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What water should I use to prepare the buffer concentrates?
FAQ ID - 3986
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What is the new Waste Tube made of?
FAQ ID - 3987
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Are the QIAwave kits based on a different chemistry than the legacy kits?
FAQ ID - 3990
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Is there a way to compare the environmental impacts of the kit?
FAQ ID - 3991
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Can the QIAwave kits be recycled?
FAQ ID - 3992
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Does the reuse of the Waste Tubes increase the risk of cross-contamination?
FAQ ID - 3988
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I don’t have any glassware in the lab, is the QIAwave kit still a good option for me?
FAQ ID - 3989
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Is the Reagent Bottle Rack, Grey, QC2 (cat. no. 9026197) compatible with the QIAcube Classic instrument?
FAQ ID - 3993
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Is the kit compatible with unpurified in-process samples?
FAQ ID - 3994
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My samples are expected to have low concentrations. Can I load more sample into the workflow or more lysate into the PCR reaction?
FAQ ID - 3995
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Can I use Internal Control as overall control?
FAQ ID - 3996
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How long can I store the reconstituted Internal Control and the reconstituted Positive Control?
FAQ ID - 3997
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Do you recommend storage of highly diluted mycoplasma samples?
FAQ ID - 3998
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What are the major differences between QIAseq Targeted RNA Panel TCR kit and previous QIAseq Immune Repertoire RNA library kit?
FAQ ID - 3999
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On which instrumentation will the RT² Profiler PCR Array work?
FAQ ID -2719
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Investigator Quantiplex: Which version of the Applied Biosystems 7500 Real-Time PCR System software can be used to run the Investigator Quantiplex?
FAQ ID -2569
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Investigator Quantiplex: Do I have to calibrate new dyes on my Applied Biosystems 7500 or 7500 Fast Real-Time PCR System?
FAQ ID -2571
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Which human target is used for the quantification in the Investigator Quantiplex Kit?
FAQ ID -2577
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Investigator Quantiplex Pro: On which real-time cyclers is the Investigator Quantiplex Pro Kit validated?
FAQ ID - 3728
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Investigator Quantiplex and Quantiplex Pro: What do I have to consider if I am using Applied Biosystems SDS software version 1.2.3?
FAQ ID -2570
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Investigator Quantiplex Pro: Which real-time cycler software version can be used to run the kit?
FAQ ID - 3730
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Investigator Quantiplex Pro RGQ: Which real-time cycler software version can be used to run the kit?
FAQ ID - 3743
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Investigator Quantiplex Pro RGQ: Which Rotor-Gene Q System can be used to run the kit?
FAQ ID - 3742
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Investigator Quantiplex Pro: Do I have to calibrate new dyes on my Applied Biosystems 7500 or QuantStudio 5 Real-Time PCR System?
FAQ ID - 3732
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How long does a run take?
FAQ ID -2566
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Investigator Quantiplex: For which real-time cyclers has the kit been validated?
FAQ ID -2567
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What are the storage conditions?
FAQ ID - 3748
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Investigator Quantiplex: Which version of the Rotor Gene-Q software can be used to run the kit?
FAQ ID -2568
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How can I streamline the quantification workflow?
FAQ ID - 3738
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Investigator Quantiplex Pro: Can the kit be run on the Rotor-Gene Q?
FAQ ID - 3729
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Investigator Quantiplex Pro RGQ: On which real-time cyclers is the kit validated?
FAQ ID - 3741
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Can I optimize a dPCR assay on the QIAcuity using gradient temperature?
FAQ ID - 3783
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Are TaqMan® Gene Expression Assays from Applied Biosystems compatible with Rotor-Gene Probe Kits?
FAQ ID -2126
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Are probes other than TaqMan® probes, such as FRET probes, compatible with fast cycling with the Rotor-Gene Multiplex PCR Kit?
FAQ ID -2130
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Are TaqMan® Gene Expression Assays from Applied Biosystems compatible with the Rotor-Gene Multiplex PCR Kit?
FAQ ID -2131
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What is the difference between QIAGEN Protease and QIAGEN Proteinase K provided in various QIAamp Kits?
FAQ ID -761
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Can I use REPLI-g for SNP Genotyping?
FAQ ID -700
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Can other probe technologies besides TaqMan® be used with the Type-it Fast SNP Probe PCR Kit?
FAQ ID -2058
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What downstream applications have been tested with DNA purified using the PAXgene Blood DNA System?
FAQ ID - 3506
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Can the QuantiFast Pathogen PCR +IC Kit or the QuantiFast Pathogen RT-PCR +IC Kit be used with hybridization probes?
FAQ ID -2595
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Can I use the QuantiTect Multiplex PCR Kits on the Roche LightCycler systems with TaqMan® probes or QuantiTect Assays?
FAQ ID -717
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Can the QuantiFast Multiplex PCR Kits be used on Roche LightCycler systems with TaqMan® probes?
FAQ ID -1979
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Are TaqMan® Gene Expression Assays from Applied Biosystems compatible with QuantiFast Probe PCR Kits?
FAQ ID -1452
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How is "Touchdown PCR" used to increase PCR specificity?
FAQ ID -75
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What should the starting template DNA quality and quantity be for PCR?
FAQ ID -74
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What kind of PCR products can be cloned with the QIAGEN PCR Cloning Kit?
FAQ ID -165
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Why do I get smeared PCR products?
FAQ ID -87
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Does QIAGEN sell Q-Solution separately?
FAQ ID -204
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Do CoralLoad dyes supplied in various QIAGEN PCR Kits interfere with downstream applications?
FAQ ID -1745
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Is Q-Solution required for PCR with QIAGEN's PCR kits?
FAQ ID -380
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Have you tested the effect of inhibitors on PCR performance?
FAQ ID -818
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What is the composition of the QIAGEN 10x PCR Buffer in Taq- and HotStarTaq DNA Polymerase Kits?
FAQ ID -606
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Do you have to use a restriction enzyme to relieve ITR secondary structures and increase target accessibility?
FAQ ID - 3974
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What is the recommended HpaII concentration?
FAQ ID - 3976
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Can I use the lysate from the CGT Viral Vector Lysis kit and combine it with another PCR system?
FAQ ID - 3979
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Do you have any recommendations on how to process my AAV samples?
FAQ ID - 3860
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What can be done if lower and upper alignment markers used with the QIAxcel System or QIAxcel Advanced are not aligned correctly?
FAQ ID -1834
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How do I safely inactivate biohazardous flow-through material?
FAQ ID -12
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How should I store plant material for DNA isolation using the DNeasy Plant Kit?
FAQ ID -114
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How can I precipitate genomic DNA using isopropanol?
FAQ ID -2953
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How do I perform a DNA precipitation to concentrate my sample?
FAQ ID -305
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What can be used as an alternative to the A260 measurement for quantification of small amounts of RNA and DNA?
FAQ ID -728
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What is the composition of buffer AE?
FAQ ID -730
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Do I need to have EDTA in the buffer in which I am going to store my isolated genomic DNA?
FAQ ID -754
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Do you have a protocol for the isolation of DNA from tofu?
FAQ ID -932
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Does the Epitect Bisulfite Kit also work on DNA extracted from plants?
FAQ ID -1209
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What QIAGEN kit can I use to isolate DNA from food products to test for genetically modified organisms (GMOs)?
FAQ ID -371
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After bisulfite conversion, can the DNA be stored?
FAQ ID -2409
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