Transfection Cell Database - Detailed View


Cell Line: H1299

Cell Line Species/Tissue: Human / Lung Cancer cell line 
Transfection Reagent: Effectene 
Nucleic Acid:
DNA 
Growth Medium:
RPMI 
Percent Serum (%):
10 
Reporter System:
Luciferase, GFP 
Plasmid Purification Method:
QIAGEN Plasmid Kit 
Plate Format:
6-well plate 
Number of Cells:
300,000 
Percent Confluence(%):
90 % 
Amount of Nucleic Acid (µg):
3 µg 
Amount of Enhancer (µl):
Amount of Reagent (µl):
10 
Complex Incubation on Cells (hrs):
Analysis Performed Post-Transfection (hrs):
48 
Transfection Efficiency (%):
40 
 
Any modifications to the protocol?:
 
Notes:
 
References: