| Cell Line Species/Tissue: |
Monkey
/
Kidney |
| Transfection Reagent: |
PolyFect |
Nucleic Acid:
|
DNA |
Growth Medium:
|
DMEM Hi glucose+FBS |
Percent Serum (%):
|
10% |
Reporter System:
|
WB |
Plasmid Purification Method:
|
QIAGEN Plasmid Kit |
Plate Format:
|
6-well plate |
Number of Cells:
|
75000 |
Percent Confluence(%):
|
60% |
Amount of Nucleic Acid (µg):
|
1.5µg |
Amount of Enhancer (µl):
|
|
Amount of Reagent (µl):
|
10µl |
Complex Incubation on Cells (hrs):
|
48h |
Analysis Performed Post-Transfection (hrs):
|
48h |
Transfection Efficiency (%):
|
60-70% |
| |
| Any modifications to the protocol?:
|
| Number of cells |
| Notes: |
| |
| References: |
| |