| Cell Line Species/Tissue: |
Human
/
|
| Transfection Reagent: |
HiPerFect |
Nucleic Acid:
|
siRNA (dsRNA) |
Growth Medium:
|
MEMalpha |
Percent Serum (%):
|
10% FCS |
Reporter System:
|
|
Plasmid Purification Method:
|
|
Plate Format:
|
12-well |
Number of Cells:
|
20.000 cells/well |
Percent Confluence(%):
|
|
Amount of Nucleic Acid (µg):
|
100nM |
Amount of Enhancer (µl):
|
|
Amount of Reagent (µl):
|
6µl |
Complex Incubation on Cells (hrs):
|
72 h |
Analysis Performed Post-Transfection (hrs):
|
72 h |
Transfection Efficiency (%):
|
|
| Knockdown Efficiency (%): |
100% |
| |
| Any modifications to the protocol?:
|
| 500µl Transfection medium, Medium change after 6h |
| Notes: |
| |
| References: |
| |