| Cell Line Species/Tissue: |
Rat
/
Insulinoma Tumor |
| Transfection Reagent: |
TransMessenger |
Nucleic Acid:
|
siRNA (dsRNA) |
Growth Medium:
|
RPMI1640 + glucose + Pen/Strep |
Percent Serum (%):
|
10% FBS |
Reporter System:
|
|
Plasmid Purification Method:
|
|
Plate Format:
|
12-well |
Number of Cells:
|
|
Percent Confluence(%):
|
50 % |
Amount of Nucleic Acid (µg):
|
0.8 µg |
Amount of Enhancer (µl):
|
1.6 µl |
Amount of Reagent (µl):
|
4 µl |
Complex Incubation on Cells (hrs):
|
4 h |
Analysis Performed Post-Transfection (hrs):
|
24-72 h |
Transfection Efficiency (%):
|
Not determined |
| Knockdown Efficiency (%): |
Worked |
| |
| Any modifications to the protocol?:
|
| |
| Notes: |
| |
| References: |
| |