Product FAQ - QIAGEN



Product FAQ Bookmark Share more.. You are not authorized to download the resource Sort options Sort alphabetically Sort by relevance How pure should my protein be with the NexTal CubicPhase kits? FAQ ID -2414 View How many samples can be processed with the QIAsymphony PAXgene Blood RNA Kit? FAQ ID -2497 View How many samples can be processed with the PAXgene Blood RNA MDx Kit? FAQ ID -2490 View In the REST software, what is the range indicated by 'Std. Error' on the 'Results' page? FAQ ID -2455 View How many PAXgene Blood RNA Tubes can be processed per PAXgene 96 Blood RNA Kit well plate? FAQ ID -2485 View If less than 48/96 samples are being processed, is it possible to leave positions empty with the PAXgene Blood RNA MDx Kit? FAQ ID -2492 View How does the DNA Protection work in the EpitTect Bisulfite kits? FAQ ID -2410 View How is the hypothesis test performed in the REST software? FAQ ID -2459 View How do I determine if my gene expression results are statistically significant in the REST software? FAQ ID -2458 View How many different genes and gene mutations are on a Somatic Mutation PCR Array? FAQ ID -2427 View How long does a QuantiFast Pathogen + IC run take on the Rotor-Gene Q? FAQ ID -2452 View How do I know in which phase of my NeXtal CubicPhase cristallisation experiment I am? FAQ ID -2421 View How great is the enrichment of miRNA when using the dedicated PAXgene Blood miRNA Kit compared to the PAXgene Blood RNA Kit? FAQ ID -2495 View How do I harvest the crystals from meso phase? Are special tools needed? FAQ ID -2422 View View Why are two reagents used in the PAXgene Tissue System? FAQ ID -3022 View Have primer sequences in the Investigator IDplex GO! Kit been changed compared to the Investigator IDplex Plus assay? FAQ ID -3092 View Is it possible to increase the amount of sample to obtain higher signals using the Investigator IDplex GO! Kit? FAQ ID -3090 View Do you recommend using indicator cards for buccal cells on FTA with Investigator IDplex GO! Kit? FAQ ID -3095 View Can the Investigator IDplex GO! PCR protocol be run on different PCR endpoint cyclers? FAQ ID -3089 View Do I need to evaluate optimal PCR cycle numbers for the Investigator IDplex GO! Kit with my samples? FAQ ID -3091 View Can additional positive controls be used for the Investigator IDplex GO! Kit? FAQ ID -3094 View Can the allelic ladder of the Investigator IDplex Plus Kit be used for analysis of Investigator IDplex GO! Kit? FAQ ID -3093 View What is the maximum size of tissue that can be fixed in a PAXgene Tissue FIX Container (50 ml)? FAQ ID -3024 View 5. What is the maximum size of the tissue sample that can be fixed in a PAXgene Tissue Container? FAQ ID -3023 View What happens if a tissue sample larger than 20 x 20 x 20 mm is used for fixation with the PAXgene Tissue FIX Container (50 ml)? FAQ ID -3025 View How long is the fixation time when using PAXgene Tissue FIX Container? FAQ ID -3026 View Is it possible to fix over a weekend? FAQ ID -3027 View How long can a tissue specimen stay in the PAXgene Tissue STABILIZER? FAQ ID -3028 View What is the minimum incubation time required when using the PAXgene Tissue STABILIZER? FAQ ID -3029 View Is it possible to integrate the PAXgene Tissue STABILIZER into the processing? FAQ ID -3037 View Is it possible to archive PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue? FAQ ID -3038 View Is the morphology after H&E staining comparable to formalin-fixed samples? FAQ ID -3039 View Are immunohistochemistry (IHC) assays developed for formalin-fixed, paraffin-embedded (FFPE) compatible with PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue? FAQ ID -3040 View Is it possible to use formalin-fixed, paraffin-embedded (FFPE) kits and protocols for extraction of biomolecules from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue? FAQ ID -3043 View is the purity of nucleic acids isolated with the PAXgene Tissue Kits? FAQ ID -3044 View What is the integrity of RNA from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue? FAQ ID -3046 View What is the average RNA yield from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue? FAQ ID -3045 View What is the RT-PCR performance of RNA purified from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue compared to RNA from snap frozen or formalin-fixed, paraffin-embedded (FFPE) tissue? FAQ ID -3050 View What is the PCR performance of DNA purified from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue compared to DNA from snap frozen or formalin-fixed, paraffin-embedded (FFPE) tissue? FAQ ID -3051 View How can proteins purified from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue be analyzed? FAQ ID -3052 View What is the DNA integrity from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue? FAQ ID -3047 View Does the PAXgene Tissue RNA Kit co-purify small RNAs? FAQ ID -3048 View How can I extract proteins from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue? FAQ ID -3049 View Can PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue be used for in situ hybridization? FAQ ID -3042 View What are the typical yields obtained from Buccal swabs on BioSprint 96? FAQ ID -3071 View Can I use a USB drive to transfer protocols to BioSprint? FAQ ID -3072 View Is the Fast Reaction Mix ready to use in the Investigator IDplex GO? FAQ ID -3087 View Is it possible to manually process PAXgene Tissue treated samples without using an automated processor? FAQ ID -3036 View Are PAXgene Tissue Containers suitable for long term-storage at freezing temperatures? FAQ ID -3030 View Is it possible to process formalin-fixed and PAXgene Tissue fixed samples together in one run? FAQ ID -3033 View Is it necessary to clean a processor normally used for formalin-fixed tissue before using it with PAXgene Tissue fixed tissue? FAQ ID -3034 View Is a special processing protocol needed? FAQ ID -3035 View Can sections of PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue be used for other histochemical staining techniques, such as PAS? FAQ ID -3041 View Are the multicopy reference assays for Type-it CNV PCR +qC Kits and the qBiomarker assays the same? FAQ ID -3000 View How do I clean up RNA preparations containing miRNA? FAQ ID -3002 View What is the fixation method used in the PAXgene Tissue System? FAQ ID -3019 View What does PAXgene Tissue FIX contain? FAQ ID -3020 View Can I perform the IDH 1/2 RGQ PCR test with slides containing less than 40% tumor cells? FAQ ID -3098 View Can the MaXtract High Density Tube be used with QIAzol to isolate RNA? FAQ ID -1304 View What volume of bacterial culture can be processed using the QuickLyse Miniprep Kit? FAQ ID -1357 View What factors determine if denatured proteins accumulate underneath, or inside the gel of the MaXtract Low and High Density Tubes? FAQ ID -1312 View I have a Human Druggable Genome siRNA Set V2.0. Can I reorder the siRNAs from this set? FAQ ID -1366 View If both MaXtract Low Density and High Density is suitable for an application, which type is preferable to use? FAQ ID -1310 View How will service work carried out on my QIAGEN BioRobot be documented? FAQ ID -1331 View If I cannot order FlexiPlate siRNA online, how do I order? FAQ ID -1371 View What information about my gene or siRNA do I need to order FlexiPlate siRNA? FAQ ID -1365 View Is it possible to use MaXtract for an organic extraction with a mixture of phenol and BCP (1 bromine chlorpropane)? -3 View What information is provided with FlexiPlate siRNAs? FAQ ID -1363 View Is it possible to elute plasmid DNA obtained with the QuickLyse Miniprep Kit in nuclease-free water? FAQ ID -1355 View Which formats are available for FlexiPlate siRNA? FAQ ID -1358 View When isolating DNA from plant cells using CTAB, should MaXtract Low or High Density be used? FAQ ID -1308 View Which parts are exchanged during a Preventive Maintenance visit for my QIAGEN BioRobot? FAQ ID -1334 View Which controls can I choose for FlexiPlate siRNA? FAQ ID -1368 View Which density gel type is contained in the yellow or green MaXtract Tubes? FAQ ID -1315 View What is the composition of FlexiPlate siRNA buffer? FAQ ID -1361 View Which software updates are included in a Premium Cover Agreement for my QIAGEN Instrument? FAQ ID -1336 View Can MaXtract tubes be autoclaved prior to use? FAQ ID -1305 View Can I reduce the amount of starting material below 10 ng when using the REPLI-g UltraFast Mini Kit? FAQ ID -1325 View Can I reduce the reaction time to e.g., 30 minutes, when using the REPLI-g UltraFast Mini Kit? FAQ ID -1326 View Can I use any type of DNA extraction method to purify template DNA for amplification using the REPLI-g Mitochondrial DNA Kit? FAQ ID -1321 View Can several extraction steps be performed in the same MaXtract tube? FAQ ID -1302 View Can a phenol-chloroform ratio different from 1:1 also be used with MaXtract Low and High Density? FAQ ID -1309 View Can DNA isolated with MaXtract tubes be used for downstream applications such as restriction digestion, labeling, blotting, PCR, and automated sequencing? FAQ ID -1303 View Can I amplify mitochondrial DNA directly from whole blood or cells using the REPLI-g Mitochondrial DNA Kit? FAQ ID -1319 View Can I order Alexa Fluor labeled controls with FlexiPlate siRNA? FAQ ID -1369 View Can HP Validated siRNAs be ordered on the FlexiPlate? FAQ ID -1367 View Can frozen sample material pulverized in liquid nitrogen be directly added to MaXtract tubes? FAQ ID -1301 View Can I also screen large constructs like BACs, fosmids, and cosmids using the QIAGEN PlasmidAmp Kit? FAQ ID -1337 View How much REPLI-g amplified mitochondrial DNA should be used in a subsequent PCR reaction? FAQ ID -1320 View Do mutations in the target mitochondrial DNA prevent primer binding, resulting in no DNA amplification with the REPLI-g Mitochondrial DNA Kit? FAQ ID -1323 View Do you have stability data for genomic DNA isolated with the BioSprint Kits? FAQ ID -1318 View Do you have stability data for genomic DNA isolated with the Flexigene DNA Kit? FAQ ID -1317 View Does the addition of sample and organic solution to the MaXtract Tubes require a specific pipetting sequence? FAQ ID -1307 View How can I upload my gene list for FlexiPlate siRNA to GeneGlobe? FAQ ID -1364 View How does a Service Support Agreement provide defined budgeting for maintaining my BioRobot? FAQ ID -1330 View How can I ensure optimal performance of my QIAGEN instrument? FAQ ID -1329 View Is it possible to purify viral RNA using FastLane Cell RT-PCR Kits? FAQ ID -1484 View Why is the activation time for HotStarTaq Plus Polymerase in the QuantiFast SYBR Green Kits different from that for QuantiFast Probe Kits? FAQ ID -1449 View Why is the RT step with the QuantiFast RT Kits much shorter compared to QuantiTect RT Kits? FAQ ID -1451 View Why is the storage time for QuantiFast PCR Kits shorter than that for QuantiTect PCR Kits? FAQ ID -1446 View Why is the reaction volume for QuantiFast PCR Kits lower than that for QuantiTect PCR Kits? FAQ ID -1447 View Why is the QuantiFast denaturation step different for PCR and RT-PCR runs in the two-step protocol for the ABI 7500 and other cyclers? FAQ ID -1442 View What is the minimum number of siRNAs per order? If I order 40 siRNAs, can I get those on 2 plates? FAQ ID -1468 View Can purification columns from other suppliers be processed on the QIAcube? FAQ ID -1406 View Can QIAcube protocol runs be interrupted, and subsequently be continued to completion? FAQ ID -1414 View Can I use more than 4 µl FastLane lysate in subsequent real-time RT-PCR? FAQ ID -1480 View Are FlexiPlate siRNAs available at a higher final concentration than 10 µM? FAQ ID -1476 View Can different amounts of siRNA be ordered on one FlexiPlate? FAQ ID -1473 View Can 2 µl reaction volumes be used with QuantiFast PCR Kits? FAQ ID -1440 View Do the QuantiTect Primer Assays work with QuantiFast SYBR Green Kits? FAQ ID -1439 View Do special settings have to be used for QuantiFast PCR Kits on the Eppendorf Mastercycler ep realplex? FAQ ID -1437 View What information is required to reorder specific siRNAs from a siRNA Set in FlexiPlate format? FAQ ID -1463 View What are the turnaround times for FlexiPlate siRNA orders? FAQ ID -1478 View How much time will be saved when switching from standard cycling to fast cycling with QuantiFast Kits? FAQ ID -1438 View How many reactions can I perform with the new QuantiFast Kits for real-time PCR? FAQ ID -1425 View If FlexiPlate siRNA was accidentally redissolved in siRNA Suspension Buffer instead of water, resulting in a 2x buffer, would it affect performance of the siRNAs? FAQ ID -1477 View How do you achieve fast cycling, yet still deliver the same performance in PCR as that achieved with standard cycling? FAQ ID -1430 View Is it necessary to place the lids of the elution tube and column into the slots of the rotor adapter during processing on the QIAcube? FAQ ID -1415 View Why do QuantiFast SYBR Green PCR Kits require such a high primer concentration? FAQ ID -1443 View What QuantiFast Kit should be used on the Eppendorf Mastercycler ep realplex? FAQ ID -1436 View What is the detection limit of the QuantiFast Kits for real-time PCR? FAQ ID -1432 View Which kind of 96 and 384-well plates are available for FlexiPlate siRNAs? FAQ ID -1459 View Which cell lines have been tested with FastLane Cell RT-PCR Kits? FAQ ID -1485 View Can I adjust the ROX concentration in the QuantiFast master mix? FAQ ID -1427 View Can I follow the reaction setup for probe-based assays described in Appendix D of the FastLane Cell RT-PCR Handbook using 384-well plates? FAQ ID -1482 View Can FlexiPlate siRNAs be ordered in plates other than the standard NUNC 96 well V bottom plates? FAQ ID -1458 View Can fast cycling be carried out with QuantiFast Probe PCR Kits and FRET probes? FAQ ID -1433 View Can FlexiPlate siRNA be ordered in solution on dry-ice? FAQ ID -1474 View How do QuantiFast PCR Kits compare to QuantiTect PCR Kits for quantitative real-time PCR? FAQ ID -1441 View How can I get software updates for the QIAcube? FAQ ID -1413 View Do you offer trial-kit sizes for the new QuantiFast Kits? FAQ ID -1429 View Can the QIAcube rotor adapters be autoclaved? FAQ ID -1409 View Can QuantiFast PCR Kits be used on real-time PCR instruments without fast cycling options? FAQ ID -1428 View Does the high primer concentration required by QuantiFast SYBR Green PCR Kits impair annealing specificity? FAQ ID -1444 View Does the master mix in the QuantiFast Kits contain dUTP to allow UNG treatments? FAQ ID -1431 View Can the QuantiFast Probe PCR +ROX Vial mastermix be stored long-term after adding ROX dye? FAQ ID -1435 View What are important points regarding the genomic DNA wipeout buffer in the QuantiFast Probe RT-PCR Plus Kit? FAQ ID -2359 View Will the sequence of the QuantiFast Probe Assays be provided? FAQ ID -2369 View Why are samples centrifuged at 4°C after addition of chloroform to the QIAzol lysates in following kits: RNeasy Lipid Tissue Mini/Midi kits, RNeasy Microarray Tissue Mini kit, RNeasy Plus Universal Mini/Midi kit, RNeasy 96 Universal Tissue kit, miRNeasy Mini kit, miRNeasy Micro kit, miRNeasy 96 kit and miRNeasy Serum/Plasma kit? FAQ ID -2345 View Why are there various different QuantiFast Probe Assays for my gene (previous versions, transcript variants)? FAQ ID -2366 View Why are the QuantiFast Probe Assays located on a single exon, thereby also detecting genomic DNA? FAQ ID -2365 View What are the main differences between the qBiomarker PCR Arrays and the RT2 Profiler PCR Arrays? FAQ ID -2438 View What are the recommended storage conditions of the QuantiFast Probe RT-PCR Plus Kit components? FAQ ID -2355 View What are the differences between the existing QuantiFast Probe RT-PCR Kit and the new Plus Kit? FAQ ID -2358 View What is the concentration of the primers and the probe in a reaction using the QuantiFast Probe Assays? FAQ ID -2370 View What is the average length of a QuantiFast Probe Assay? FAQ ID -2374 View Is the RNeasy Plus Universal Mini Kit suited for adipose, brain, and other fatty tissues? FAQ ID -2343 View Is there a size limit to the protein I can crystallize with the NexTal Cubic Phase products? FAQ ID -2331 View Is the T790M mutation included in the EGFR PCR kit? FAQ ID -2314 View Is it necessary to perform calibration steps for the use of the MAX dye in duplex RT-PCR experiments on different cyclers? FAQ ID -2373 View Is there also a miRNeasy Plus Universal Kit available? FAQ ID -2346 View I use cultured cells as starting material. Can I also use the RNeasy Plus Universal Kit? FAQ ID -2342 View I would like to order the QuantiFast Probe Assays without master mix. Is this possible? FAQ ID -2360 View My ADAPT report states "Run Invalid" and does not display any sample results. Why is this? FAQ ID -2306 View Is V600E the only mutation that can be detected with the codon 600 assay in the BRAF Pyro Kit? FAQ ID -2382 View Is the yield and quality of RNA and DNA purified with the AllPrep DNA/RNA FFPE Kit comparable to yield and quality of nucleic acids purified separately with other methods. FAQ ID -2349 View Which purification kits are recommended for the Pyro kits? FAQ ID -2380 View Which kit, the QIAprep 96 Plus Miniprep Kit or the QIAGEN Plasmid Plus 96 Miniprep Kit, do you recommend for transfection? FAQ ID -2320 View Which probe labels are available for the QuantiFast Probe Assays? FAQ ID -2361 View What should be the concentration of my protein with the NexTal Cubic Phase products? FAQ ID -2325 View What is the maximum plasmid size which I can use with the QIAprep 96 Plus Miniprep Kit or the QIAGEN Plasmid Plus 96 Miniprep Kit? FAQ ID -2316 View What plug-ins are available for the Pyrosequencing kits? FAQ ID -2381 View Which crystallization solutions are compatible with lipidic cubic phase crystallization? FAQ ID -2418 View Which deparaffinization method can I use with the AllPrep DNA/RNA FFPE Kit? FAQ ID -2352 View What species are QuantiTect Probe Assays offered for? FAQ ID -2367 View Can the insertions in Exon 20 be detected with the EGFR Pyro Kit? FAQ ID -2383 View Is it also possible to isolate miRNA with the AllPrep DNA/RNA FFPE Kit? FAQ ID -2348 View Can I use the RNeasy Plus Universal Mini Kit for all types of tissue? FAQ ID -2338 View Can the QIAprep 96 Plus Miniprep Kit or the QIAGEN Plasmid Plus 96 Miniprep Kit be used for low-copy number plasmids? FAQ ID -2317 View Can the QuantiFast Probe Assay detect short fragmented RNA as well as longer fragments of RNA? FAQ ID -2364 View Can negative crossing threshold (CT) values be negative when using the KRAS and EGFR PCR kits? FAQ ID -2300 View Can QuantiFast Probe Assays be ordered offline via e-mail or fax? FAQ ID -2368 View For the the QIAprep 96 Plus Miniprep Kit or the QIAGEN Plasmid Plus 96 Miniprep Kit, can plates be partially used? FAQ ID -2319 View For which applications can the RNA isolated with the RNeasy Plus Universal Kit also be used? FAQ ID -2339 View Does the KRAS PCR kit cover the codon 61 mutation? FAQ ID -2308 View Are the QIAprep 96 Plus Miniprep Kit or the QIAGEN Plasmid Plus 96 Miniprep Kit suited for short hairpin vectors? FAQ ID -2318 View Are the QuantiFast Probe Assays hydrolysis or FRET probes? FAQ ID -2363 View Are the buffers in the RNeasy QIAcube kit delivered in QIAcube buffer bottles? FAQ ID -2334 View Are QIAshredder spin columns delivered with the RNeasy Mini QIAcube Kit? FAQ ID -2333 View Can I do singleplex PCR with the QuantiFast Probe Assays? FAQ ID -2362 View Can I use more than 10ng DNA for the PCR reaction for the Pyro kits? FAQ ID -2379 View Are the QuantiFast Probe Assays wet-lab validated? FAQ ID -2375 View At what temperature can I perform the experiment with the NexTal Cubic Phase kits? FAQ ID -2323 View How much can the mixed standard ?Ct values vary in the KRAS PCR kit? FAQ ID -2307 View How many samples can be processed per RNeasy Mini QIAcube kit? FAQ ID -2332 View How much DNA/RNA can be isolated with the AllPrep DNA/RNA FFPE Kit? FAQ ID -2353 View How many freeze-thaw cycles can the master mix contained in the QuantiFast Probe RT-PCR Plus Kit tolerate? FAQ ID -2357 View How pure should the chloroform used with the RNeasy Plus Universal Mini Kit be? FAQ ID -2344 View How much sample material can I use for DNA/RNA purification with the AllPrep DNA/RNA FFPE Kit? FAQ ID -2351 View How much time can be saved when using the RNeasy Mini QIAcube Kit? FAQ ID -2335 View How does the QuantiFast Probe RT-PCR Plus Kit eliminate genomic DNA contamination? FAQ ID -2354 View How do I analyze the data for the particular iPSC qBiomarker Arrays I have ordered? FAQ ID -2440 View How are the genes in each iPSC qBiomarker PCR Array selected? FAQ ID -2439 View How can I adjust the ROX concentration in the QuantiFast Probe RT-PCR Plus master mix? FAQ ID -2356 View How do I harvest the crystals from meso phase? Are special tools needed with the NexTal Cubic Phase products? FAQ ID -2330 View What is the advantage of EpiTect MethyLight PCR Kits over QuantiTect Probe PCR Kits for methylation studies? FAQ ID -2010 View What is the advantage of using the Type-it Microsatellite PCR Kit? FAQ ID -2059 View What is a good starting amount of template DNA for PCR with the EpiTect MSP Kit? FAQ ID -2005 View What is the advantage of using the Type-it Mutation Detect PCR Kit? FAQ ID -2064 View What is the maximum lifetime of the QIAGEN rotor for the Centrifuge 4 and 4K? -16 View What is the principle behind the DNA Polymerase in the EpiTect MSP Kit? FAQ ID -2003 View What is the maximum DNA yield from forensic samples processed with the QIAsymphony SP? FAQ ID -2028 View Is it possible to use different sample types in one run with the MagAttract 96 cador Pathogen Kit? FAQ ID -2072 View Is magnetic particle carry-over in the eluates on the BioSprint 96 normal? FAQ ID -2073 View What happens when using EpiTect MethyLight Assays on only partially bisulfite converted DNA template? FAQ ID -2009 View What are the expected PCR results when using EpiTect Control DNA on untreated or bisulfite-converted DNA? FAQ ID -2011 View What are the features of the expression vector used for QIAgenes Insect/Mammalia? FAQ ID -2050 View Why is CoralLoad included in the Type-it Mutation Detect, but not in the Type-it Microsatellite PCR Kit? FAQ ID -2068 View Why do casework-sample protocols for the QIAsymphony DNA Investigator Kit start with different lysate volumes (200 µl, 500 µl, or 1000 µl)? FAQ ID -2035 View Why do pQE-T7 vectors of the QIAgenes E. coli system have an additional UAG amber stop codon? FAQ ID -2043 View Why is the Type-it Mutation Detect PCR Kit recommended for preamplification of SNPs? FAQ ID -2067 View Why is maximum amplicon size for the Type-it Microsatellite PCR Kit limited to 500 bp? FAQ ID -2060 View Will the optimized DNA sequence for QIAgenes be provided? FAQ ID -2045 View What sample types can be processed using the QIAsymphony Virus/Bacteria protocols? FAQ ID -2036 View What sample volumes can be processed with the QIAsymphony Virus/Bacteria Kits? FAQ ID -2037 View Can data be analyzed on the Rotor-Gene Q while a run is in progress? FAQ ID -2091 View Are there recommendations for sensitive detection of underrepresented fragments using the Type-it Mutation Detect PCR Kit? FAQ ID -2066 View Are there two vectors for protein expression using QIAgenes in insect or mammalian cells? FAQ ID -2048 View Is mitochondrial DNA isolated with the QIAsymphony DNA Investigator Kit on the QIAsymphony SP? FAQ ID -2030 View Is it possible to edit the sample sheet in the Rotor-Gene Q software after a PCR run? FAQ ID -2092 View Is it possible to import a standard curve from a previous PCR run on the Rotor-Gene Q? FAQ ID -2093 View What kind of samples should be processed using the large-volume protocol with the QIAsymphony DNA Investigator Kit? FAQ ID -2034 View Which QIAsymphony Virus/Bacteria Kit size should be used for each protocol? FAQ ID -2038 View Which expression systems can be used with the QIAgenes Insect/Mammalia expression constructs? FAQ ID -2049 View What types of reaction vessels are required for use in the Rotor-Gene Q? FAQ ID -2085 View Which capillary sequencers can be used for detection of amplicons from the Type-it Microsatellite PCR Kits? FAQ ID -2063 View Can the EpiTect PCR Control DNAs be used for mouse and rat assays? FAQ ID -2008 View Can the EpiTect PCR Control DNAs also be used as standard in real-time methylation specific quantification? FAQ ID -2006 View Can the protocols for purification of small RNAs from cells in the appendices of the AllPrep DNA/RNA 96 Handbook also be used with tissue samples? FAQ ID -2001 View Can QIAGEN rotors used with the centrifuges 4C and 4KC also be used with 4-16 and 4-16K models? -15 View Can the MagAttract 96 cador Pathogen Kit also be used on the KingFisher 96 or MagMAX Express 96 instruments? FAQ ID -2074 View Can TopTaq Master Mix simply be stored at room temperature on the lab bench for up to 4 months? FAQ ID -2052 View Do all QIAsymphony DNA Investigator protocols use carrier RNA? FAQ ID -2033 View Can the Type-it Microsaltellite PCR Kit be used on cyclers with fast ramping rates? FAQ ID -2062 View Can the Type-it Mutation Detect PCR kit be used for multiplex PCR of fragments longer than 500 bp? FAQ ID -2065 View Can 0.1 ml and 0.2 ml tubes used on the Rotor-Gene Q be labeled? FAQ ID -2082 View Can DNase digestion be performed on the RNeasy 96 plate during the RNA purification part of the Allprep DNA/RNA 96 procedure? FAQ ID -2000 View How many signal readings are taken during the real-time data acquisition on the Rotor-Gene Q? FAQ ID -2083 View Can membrane proteins be expressed with QIAgenes expression kits? FAQ ID -2047 View Do QIAsymphony Virus/Bacteria samples require pretreatment before they are loaded onto the instrument? FAQ ID -2039 View Does the centrifugal force have any effect on the kinetics of a PCR reaction on the Rotor-Gene Q? FAQ ID -2081 View Does an internal control have to be included for each QIAsymphony Virus/Bacteria protocol run? FAQ ID -2040 View How can full-length genes or individual domains of the QIAgenes Insect/Mammalia constructs be subcloned? FAQ ID -2051 View Can frozen samples be used with the MagAttract 96 cador Pathogen Kit? FAQ ID -2070 View How fast does the rotor spin during a run on the Rotor-Gene Q? FAQ ID -2080 View How is the log graph threshold value (Ct) for the Rotor-Gene Q determined? FAQ ID -2088 View How long can DNA converted with EpiTect Bisulfite Kits be stored before use in methylation specific PCR with the EpiTect MSP Kit? FAQ ID -2002 View How will running less than 96 samples with the MagAttract 96 cador Pathogen kit affect usage of plasticware? FAQ ID -2071 View If new dyes or chemistries appear on the real-time PCR market, will Rotor-Gene Q be capable of running those dyes? FAQ ID -2087 View If orders cannot be placed online, how can QIAgenes be ordered? FAQ ID -2046 View How much space does the PyroMark Q24 instrument need? FAQ ID -2098 View Does the Type-it Microsatellite PCR Kit always require an annealing temperature of 60°C, or can pre-optimized annealing temperatures for target-primer systems be used? FAQ ID -2061 View What is the turn around time for the ordering of miScript Target Protector? FAQ ID -2254 View What is the optimal range of PCR fragment sizes for normalization with the SeqTarget System? FAQ ID -2247 View What is the processing time for 96 normalizations with the SeqTarget System? FAQ ID -2237 View What technologies are used by the KRAS and EGFR PCR kits? FAQ ID -2286 View What method do you recommend to quantify DNA before running the KRAS and EGFR PCR kit? FAQ ID -2292 View What kind of automation can be done with the EasyXtal 15-well Tool? FAQ ID -2220 View What format are the EasyXtal 15-well tools? FAQ ID -2219 View What dyes are used in the KRAS and EGFR PCR kits? FAQ ID -2288 View What do I do if I get amplification in my no template control (NTC) with the KRAS and EGFR PCR kits? FAQ ID -2298 View What do I need to provide when ordering a miScript Target Protector? FAQ ID -2251 View What is the difference of the sequence of a miScript miRNA Inhibitor and miScript Target Protector? FAQ ID -2253 View What is a miScript Target Protector? FAQ ID -2249 View What is a miScript Precursor Assay? FAQ ID -2214 View Which transfection reagent is recommended for transfection of the miScript Target Protectors? FAQ ID -2252 View Which variants of the EasyXtal 15-well tool are available? FAQ ID -2221 View Why are most of my samples outside of the cluster in supervised mode using Rotor-Gene ScreenClust HRM Software? FAQ ID -2205 View Why are some of my samples outside of the cluster using Rotor-Gene ScreenClust HRM Software? FAQ ID -2204 View Why does the miScript Target Protector not induce the degradation process? FAQ ID -2263 View Why does the SeqTarget Prep Protocol require a 2-step procedure? FAQ ID -2238 View What will happen, if the normalized DNA for the SeqTarget System is eluted with Buffer EB instead of EB2? FAQ ID -2242 View When negative results are generated using the KRAS and EGFR PCR kits, do I rerun them? FAQ ID -2294 View What type of samples can I use for the KRAS PCR and EGFR kits? FAQ ID -2293 View What voltage should be used to run my agarose gel with the GelPilot Agarose? FAQ ID -2261 View Which mode should I use in the Rotor-Gene ScreenClust HRM Software, supervised or unsupervised? FAQ ID -2202 View Do the KRAS and EGFR PCR kits contain a passive reference dye? FAQ ID -2295 View Does the atypical look of the lyophilized FlexiTube siRNA Premix have a negative impact on performance? FAQ ID -2267 View Does the miScript Target Protector match the target sequence? FAQ ID -2250 View How do I store my FlexiTube siRNA Premix? FAQ ID -2270 View Do I have to order at least 4 FlexiTube siRNA Premixes? FAQ ID -2266 View How is ARMS technology superior to alternative methods such as dye terminator sequencing? FAQ ID -2290 View How do I search for all the miScript Target Protectors for one species? FAQ ID -2255 View How can I check the success of the normalization procedure for the SeqTarget System? FAQ ID -2245 View Can PCR fragments be normalized for the SeqTarget System after purification with QIAquick? FAQ ID -2240 View Are all 3 components of the SeqTarget System required or can a different Long Range PCR Kit and primers be used? FAQ ID -2234 View Can PCR fragments be normalized for the SeqTarget System without a previous purification step? FAQ ID -2246 View Can I use a 384 well plate for the KRAS and EGFR PCR kits? FAQ ID -2296 View Can the miScript Precursor Assay differentiate between pri- and pre-miRNA? FAQ ID -2211 View Does ethanol from the PCR product purification influence the normalization procedure for the SeqTarget System? FAQ ID -2244 View What are EasyXtal 15-well Tools used for? FAQ ID -2217 View What are Principal Components analyzed in Rotor-Gene ScreenClust HRM Software? FAQ ID -2200 View What are the amplicon lengths of the KRAS and EGFR PCR kits? FAQ ID -2289 View What are clusters analyzed in the Rotor-Gene ScreenClust HRM Software? FAQ ID -2201 View Is the SeqTarget System compatible with standard-size PCRs? FAQ ID -2241 View What controls are used in the KRAS and EGFR PCR kits to ensure reliable results? FAQ ID -2291 View What does QIAGEN use for a positive control in precursor detection experiments? FAQ ID -2212 View What do I do if I cannot see amplification in the FAM channel in my KRAS and EGFR PCR kits? FAQ ID -2297 View What are the corresponding QIAGEN names for former Biotage instruments? FAQ ID -2285 View What can be the reason for low DNA yields after normalization with the SeqTarget System? FAQ ID -2239 View How much buffer should be used for agarose gel electrophoresis? FAQ ID -2257 View How long does it take to generate results using the KRAS and EGFR PCR kits? FAQ ID -2299 View How should gels be cast with the GelPilot Agarose so that optimal resolution is achieved? FAQ ID -2258 View How long should PCR fragments be for the SeqTarget System? FAQ ID -2236 View Is it possible to use lower siRNA concentrations with FlexiTube siRNA Premix? FAQ ID -2264 View How many miScript Precursor Assays do you offer? FAQ ID -2213 View Is one able to use FlexiTube siRNA Premix for transfection of primary adherent cell types? FAQ ID -2268 View If I am working with a difficult-to-transfect cell type or if I obtain only weak silencing effects what can I do? FAQ ID -2265 View Is one able to use FlexiTube siRNA Premix for transfection of primary suspension cell types? FAQ ID -2269 View Is it possible to normalize to more or less than 500 ng with the SeqTarget System if needed? FAQ ID -2235 View How many samples can be processed with the QIAsymphony DNA Mini Kit and the Virus Blood 200 protocol? FAQ ID -2159 View How often can the Strep-Tactin Superflow Plus resin be reused? FAQ ID -2169 View Is it possible to purify and normalize long range PCRs produced with PCR kits from other vendors for the SeqTarget System? FAQ ID -2243 View If housekeeping and target genes differ significantly in abundance, will they both be amplified with equal efficiency using the Rotor-Gene Multiplex PCR Kit? FAQ ID -2129 View If Vapor-Lock is used to overlay PCR reactions, which volume should be entered as reaction volume in the Rotor-Gene Q Software? FAQ ID -2150 View Is it possible to purify a GST-tagged protein under denaturing conditions using Glutathione Superflow? FAQ ID -2172 View Is it normal that the binding capacity of GST-resins decreases with flow rate? FAQ ID -2174 View Why is the initial activation step different for Rotor-Gene Probe, SYBR Green and Multiplex Kits? FAQ ID -2118 View Will Rotor-Gene Kits also work on the Rotor-Gene 6000 and 3000 cyclers? FAQ ID -2121 View Why is there an asterisk for my miScript Primer Assays? FAQ ID -2176 View Will Uracil-N-Glycosylase (UNG) completely remove contaminating amplicons when using QuantiTect +UNG Kits? FAQ ID -2139 View Will QuantiTect Primer Assays work with Rotor-Gene SYBR Green Kits using an annealing step at 60ºC? FAQ ID -2124 View What is miRNA*? Do I need to consider this in my profiling experiment? FAQ ID -2179 View What are the main differences between Rotor-Gene and QuantiTect or QuantiFast PCR Kits? FAQ ID -2119 View What are possible reasons for reduced DNA yields with REPLI-g Kits? FAQ ID -2148 View What is the residuals plot in the Rotor-Gene ScreenClust HRM Software? FAQ ID -2199 View What is the target nucleic acid for the Virus Blood 200 Protocol on the QIAsymphony? FAQ ID -2158 View What is the detection limit of the Rotor-Gene and QuantiFast Multiplex RT-PCR Kits? FAQ ID -2144 View What is the minimum number of assays that can be ordered on QuantiTect Primer Assay Plates? FAQ ID -2109 View What is the maximum number of targets that can be amplified per reaction with the QuantiFast Multiplex RT-PCR Kit? FAQ ID -2145 View How should DNA isolated on the QIAsymphony be stored? FAQ ID -2165 View How should samples be stored before purification using the Virus Blood 200 Protocol on the QIAsymphony? FAQ ID -2157 View How pure should the chloroform used with the RNeasy Microarray Tissue Mini Kit be? FAQ ID -2193 View Why should I use the RNeasy Microarray Tissue Mini Kit when purifying RNA for microarray analysis? FAQ ID -2188 View Which elution volumes can be selected using the QIAsymphony Virus Blood 200 protocol? FAQ ID -2160 View What sample volumes can be used with the Virus Blood 200 Protocol on the QIAsymphony? FAQ ID -2156 View Which is the best condition for eluting GST-tagged proteins from Glutathione Superflow? FAQ ID -2173 View What species are QuantiTect Primer Assay Plates offered for? FAQ ID -2110 View Which plate formats are offered for QuantiTect Primer Assay Plates? FAQ ID -2106 View Why are the denaturation and annealing/extension times for QuantiFast Multiplex RT-PCR Kits much shorter than those for QuantiFast Multiplex PCR Kits? FAQ ID -2147 View Which sample types can be processed using the QIAsymphony Virus Blood 200 protocol? FAQ ID -2154 View How many reactions can be performed per well of the QuantiTect Primer Assay Plates? FAQ ID -2107 View Can assays for 20 and 100 reactions be ordered on the same QuantiTect Primer Assay Plate? FAQ ID -2108 View Can QuantiTect Primer Assay Plates be ordered offline via e-mail or fax? FAQ ID -2112 View Can Glutathione Superflow resin be reused? FAQ ID -2171 View Can Uracil-N-Glycosylase (UNG) be added to the QuantiTect PCR Master Mix and be stored for later use? FAQ ID -2138 View Can Uracil-N-Glycosylase (UNG) be purchased separately, independent of QuantiTect PCR Kits? FAQ ID -2134 View Are there any changes in the procedure using the Strep-Tactin Superflow Plus resin with the higher capacity over the standard matrix? FAQ ID -2168 View Can Uracil-N-Glycosylase (UNG) be used with a one-step RT-PCR kit, such as the QuantiTect Probe RT-PCR Kit? FAQ ID -2135 View Can the Rotor-Gene Multiplex RT-PCR Kit be used on other cyclers? FAQ ID -2142 View Can RNA isolated with the RNeasy Microarray Tissue Mini Kit also be used in downstream applications other than microarray analysis? FAQ ID -2191 View Are QuantiTect PCR +UNG Kits available in bulk format (e.g., 25 ml master mix)? FAQ ID -2136 View Are Rotor-Gene Kits compatible with reaction setup using the QIAgility instrument? FAQ ID -2116 View How does template quality influence the results with the Type-it HRM PCR Kit? FAQ ID -2197 View Are the layouts for both 96- and 384-well QuantiTect Primer Assay Plates visible when ordering online? FAQ ID -2111 View Do you have protocols for software setup on various PCR platforms using QuantiFast Probe RT-PCR Kits? FAQ ID -2105 View How does magnetic-particle carry-over in QIAsymphony eluates need to be handled? FAQ ID -2166 View How does the RNeasy Microarray Tissue Mini Kit prevent traces of phenol from negatively affecting the reverse-transcription step in microarray experiments? FAQ ID -2189 View How do Rotor-Gene Probe Kits compare with QuantiFast Probe Kits? FAQ ID -2125 View How many elution steps are recommended when using Glutathione Superflow resin? FAQ ID -2175 View Do you have a protocol for Rotor-Gene software setup for the Rotor-Gene SYBR Green PCR and RT-PCR Kits? FAQ ID -2104 View Does an internal control have to be used with the QIAsymphony Virus Blood 200 Protocol? FAQ ID -2162 View Can Vapor-Lock be used with samples processed on the QIAgility? FAQ ID -2153 View Do the master mixes in Rotor-Gene Kits contain dUTP to allow UNG pretreatment? FAQ ID -2117 View Do QuantiTect Primer Assays also work with Rotor-Gene SYBR Green PCR Kits? FAQ ID -2123 View Do limiting primer concentrations need to be determined when using the Rotor-Gene Multiplex PCR Kit? FAQ ID -2128 View What buffer should be used to dilute cDNA made using the miScript Reverse Transcription Kit? FAQ ID -1601 View Is FlexiTube GeneSolution available for other scales than the 1 nmol scale? FAQ ID -1666 View Is it necessary to clean up cDNA prepared with the QuantiTect Whole Transcriptome Kit? FAQ ID -1610 View Is the master mix of QuantiFast Kits for real-time PCR aliquoted into several tubes to prevent cross-contamination? FAQ ID -1697 View Is the QuantiTect Whole Transcriptome Kit suitable for miRNA amplification? FAQ ID -1609 View Is it necessary to include a genomic DNA removal step with the QuantiTect Whole Transcriptome Kit? FAQ ID -1614 View Is sequence information for HP Validated siRNAs provided in the FlexiTube format? FAQ ID -1665 View Where can I find background information and literature on Whole Genome Amplification with REPLI-g Kits? FAQ ID -1690 View Why is there DNA in the no-template (negative) control when using the QuantiTect Whole Transcriptome Kit? FAQ ID -1620 View What is the maximum volume of RNA in solution that can be used with the QuantiTect Whole Transcriptome Kit? FAQ ID -1616 View Can the Ni-NTA and Strep-Tactin Superflow Cartridges be reused? FAQ ID -1607 View Can the Ni-NTA Superflow Cartridges and/or Strep-Tactin Cartridges be connected in series? FAQ ID -1606 View Can the QuantiTect Whole Transcriptome Kit be used for amplification of RNA from LCM samples? FAQ ID -1612 View Can I store cDNA generated by the miScript System? FAQ ID -1602 View Does the QuantiTect Whole Transcriptome Kit work with RNA purified from bacteria, yeast, or plants? FAQ ID -1615 View Can T-Script® enzyme of the QuantiTect Whole Transcriptome Kit be substituted by Quantiscript Reverse Transcriptase? FAQ ID -1617 View Can the QuantiTect Whole Transcriptome reaction be stopped after the reverse transcription step to proceed with the ligation and amplification steps at a later time? FAQ ID -1618 View Can I still reorder siRNA that has been removed from the GeneGlobe data base? FAQ ID -1668 View Can amplification with the QuantiTect Whole Transcriptome Kit be extended to more than 8 hours to improve cDNA yields? FAQ ID -1608 View Can I select HP Validated and HP GenomeWide siRNAs for FlexiTube? FAQ ID -1658 View Can cDNA prepared with the QuantiTect Whole Transcriptome Kit be reampliied using REPLI-g Kits? FAQ ID -1611 View Can FlexiTube siRNA or FlexiTube GeneSolution products be ordered in solution? FAQ ID -1667 View How fast is the 6xHis-tagged protein purification process using Ni-NTA Superflow Cartridges? FAQ ID -1605 View How long is cDNA generated with the QuantiTect Whole Transcriptome Kit? FAQ ID -1613 View How long can I archive cDNA generated with the QuantiTect Whole Transcriptome Kit? FAQ ID -1623 View Do I need to use the miScript Universal Primer for detection of mRNA? FAQ ID -1600 View Have you observed co-amplification of genomic DNA from RNA templates used in the QuantiTect Whole Transcriptome Procedure? FAQ ID -1619 View Do you offer a QuantiFast Kit for one-step RT-PCR? FAQ ID -1695 View Do you have a protocol for purification of total RNA from plant latex? FAQ ID -1694 View Do you have a protocol for purification of total RNA from plant tissue with the RNeasy Lipid Tissue Mini Kit? FAQ ID -1693 View What effect does homogenization have on DNA yield and integrity when using AllPrep DNA/RNA Kits? FAQ ID -1751 View Is TopTaq DNA Polymerase a hot-start polymerase? FAQ ID -1748 View What is the fidelity of TopTaq DNA Polymerase? FAQ ID -1739 View Which nucleotide analogs can be used with TopTaq DNA Polymerase? FAQ ID -1741 View Why might Affymetrix GeneChip Mapping assays interfere with the REPLI-g FFPE kit? FAQ ID -1755 View What is the shelf-life of TopTaq DNA Polymerase and Master Mix? FAQ ID -1743 View What is the principle of the REPLI-g FFPE Kit? FAQ ID -1753 View What is the largest plasmid size that can be purified using the QuickLyse Miniprep Kit? FAQ ID -1752 View What is the minimum amount of starting material that can be used with the RNeasy Plus Micro and AllPrep DNA/RNA Micro Kit? FAQ ID -1750 View What is the recommended sample size for use with the REPLI-g FFPE Kit? FAQ ID -1756 View Can small miRNA-containing RNA fractions be separated from large RNAs using the miRNeasy FFPE Kit? FAQ ID -1737 View Can miRNA and other RNAs smaller than 200 nucleotides be isolated with the AllPrep DNA/RNA Micro Kit or RNeasy Plus Micro Kit? FAQ ID -1749 View Can TA/UA cloning be done with PCR products amplified with TopTaq DNA Polmyerase and TopTaq Master Mix Kits? FAQ ID -1740 View Are there problems with the default threshold setting on the Applied Biosystems 7500 when using QuantiFast Probe PCR Kits? FAQ ID -1702 View At which step in the REPLI-g FFPE protocol should PicoGreen quantification be done? FAQ ID -1758 View How can foaming of Buffer RLT Plus lysates be avoided when using RNeasy Plus Micro and Mini Kits, or the Allprep DNA/RNA Mini Kit? FAQ ID -1734 View Do you have protocols for sample processing on the Autopure LS instrument? FAQ ID -1759 View Have QuantiTect Primer Assays been tested with QuantiFast SYBR Green PCR Kits on the Mastercycler ep realplex? FAQ ID -1714 View Can the REPLI-g FFPE procedure restore DNA derived from FFPE tissue samples in the original order? FAQ ID -1754 View Can the REPLI-g FFPE Kit also be used for formalin-fixed, ethanol preserved samples? FAQ ID -1760 View Do you have a protocol for the isolation of total RNA from paraffin-embedded sections on the Biorobot M48? FAQ ID -1733 View Does TopTaq Stabilizer of the TopTaq DNA Polymerase and Master Mix Kits interfere with downstream applications? FAQ ID -1744 View Do you have a forensic post-PCR purification protocol to purify double-stranded DNA fragments from PCR reactions? FAQ ID -1761 View Is sample pretreatment required for purifying RNA on the QIAsymphony SP? FAQ ID -1938 View Is the QIAsymphony RNA Kit compatible with stabilized samples? FAQ ID -1944 View Is RNA co-purified with DNA using QIAsymphony DNA Tissue protocols? FAQ ID -1920 View Is there a charge for user training on the QIAsymphony SP System? FAQ ID -1900 View What are the expected DNA yields from tissue processed on the QIAsymphony SP? FAQ ID -1919 View QuantiTect Primer Assays are bioinformatically validated, genomewide primer sets. What does “bioinformatically validated” mean? FAQ ID -1982 View What is the range of elution volumes on the QIAsymphony SP? FAQ ID -1908 View How many samples can be processed per kit or per reagent cartridge for RNA purification on the QIAsymphony SP? FAQ ID -1940 View How does QIAsymphony SP keep cellular samples in suspension prior to pipetting so that samples do not settle? FAQ ID -1905 View How many PCR reactions can be performed for different scales of miScript Primer Assays? FAQ ID -1990 View How efficient is DNA removal by a gDNA Eliminator 96 plate compared with DNase digestion on an RNeasy 96 plate? FAQ ID -1994 View How long can homogenates remain on the QIAsymphony SP without risk of RNA degradation? FAQ ID -1941 View How much sample can be used with the Allprep DNA/RNA 96 Kit? FAQ ID -1997 View Which types and amounts of sample material can be processed for RNA extraction on the QIAsymphony SP? FAQ ID -1937 View How much starting material can be used with the QIAsymphony DNA Mini Kit on the QIAsymphony SP? FAQ ID -1917 View What sample types can be used with the AllPrep DNA/RNA 96 Kit? FAQ ID -1996 View What sample types should be processed with the High Content (HC) protocol for DNA extraction on the QIAsymphony SP? FAQ ID -1918 View What sample types can be used with the RNeasy Plus 96 Kit? FAQ ID -1991 View Will mitochondrial DNA also be isolated using the DNA Tissue protocols on the QIAsymphony SP? FAQ ID -1921 View Why does the Allprep DNA/RNA 96 Kit use Buffer RLT, whereas the AllPrep DNA/RNA Mini Kit uses Buffer RLT Plus? FAQ ID -1999 View Why is there no vacuum-only protocol for the RNeasy Plus 96 Kit? FAQ ID -1993 View What is the turn around time for custom miScript miRNA mimics, inhibitors and primer assays? FAQ ID -1985 View What kind of molecules are miScript miRNA Inhibitors? FAQ ID -1983 View Is it possible to modify QIAsymphony protocols? FAQ ID -1903 View What are the expected yields of total RNA isolated with the QIAsymphony SP? FAQ ID -1942 View What are the starting volumes of homogenate for RNA extraction on the QIAsymphony SP? FAQ ID -1939 View What sample input volumes can be used on the QIAsymphony SP? FAQ ID -1907 View What sample types can be processed using the tissue protocols for DNA extraction on the QIAsymphony SP? FAQ ID -1915 View What kind of molecules are miScript miRNA Mimics? FAQ ID -1986 View What RNA elution volumes are available with the QIAsymphony RNA Kit? FAQ ID -1943 View Do limiting primer concentrations need to be determined when using QuantiFast Multiplex PCR Kits? FAQ ID -1976 View Do samples require pretreatment before loading onto the QIAsymphony SP for DNA extraction? FAQ ID -1916 View Can uneven sample numbers be processed on the QIAsymphony SP module? FAQ ID -1906 View Does HiPerFect work for the transfection of miScript miRNA mimics and inhibitors? FAQ ID -1984 View Does the QIAsymphony SP system generate run reports and/or log files? FAQ ID -1911 View Do you have a protocol for the purification of archive-quality DNA from 100 to 5 x 10e8 cultured cells using Gentra Puregene Kits? FAQ ID -1960 View Do you have a protocol for the purification of archive-quality DNA from 0.5–20 mg paraffin-embedded tissue using Gentra Puregene Kits? FAQ ID -1964 View Do you have a protocol for the purification of archive-quality DNA from 1 ml of whole blood with the Gentra Puregene Tissue Kit? FAQ ID -1953 View Do you have a protocol for rapid purification of archive-quality DNA from up to 9 x 10e6 cells using Gentra Puregene Kits? FAQ ID -1969 View Can I program my own protocols for the QIAsymphony SP? FAQ ID -1904 View Are QuantiFast Multiplex PCR Kits available in trial sizes? FAQ ID -1981 View Can 4-plex, real-time PCR be performed on the ABI PRISM 7000, 7700, or 7900 using the QuantiFast Multiplex PCR Kit? FAQ ID -1978 View Can the QIAsymphony RNA Kit be used to purify RNA from blood? FAQ ID -1945 View Can the QIAsymphony SP Module be connected to a laboratory information management system (LIMS)? FAQ ID -1909 View Can protocol runs on the QIAsymphony SP be interrupted and resumed? FAQ ID -1912 View Can the protocols for purification of small RNAs from cells in the appendices of the RNeasy Plus 96 Handbook also be used with tissue samples? FAQ ID -1995 View Do you have a protocol for the purification of archive-quality DNA from marine invertebrate tissue using Gentra Puregene Kits? FAQ ID -1950 View Do you have a protocol for the purification of archive-quality DNA from nematodes using Gentra Puregene Kits? FAQ ID -1951 View Do you have a protocol for the purification of archive-quality DNA from human dried blood using Gentra Puregene Kits? FAQ ID -1959 View Do you have a protocol for the purification of archive-quality DNA from Gram-negative bacteria using Gentra Puregene Kits? FAQ ID -1975 View Do you have a protocol for the purification of archive-quality DNA from Gram-positive bacteria using Gentra Puregene Kits? FAQ ID -1946 View Do you have a protocol for the purification of archive-quality DNA from hair roots using Gentra Puregene Kits? FAQ ID -1947 View Do you have a protocol for the purification of DNA from fruit flies? FAQ ID -1970 View How can the QIAsymphony SP system be decontaminated? FAQ ID -1914 View Do you have a protocol for the purification of archive-quality DNA from tissue fixed in ethanol or formalin using Gentra Puregene Kits? FAQ ID -1974 View Do you have a protocol for the purification of archive-quality DNA from umbilical cord blood using Gentra Puregene Kits? FAQ ID -1958 View Do you have a protocol for the purification of archive-quality DNA from whole blood using Gentra Puregene Kits? FAQ ID -1954 View Do you have a protocol for the purification of archive-quality DNA from buffy coat using Gentra Puregene Kits? FAQ ID -1955 View Do you have a protocol for the purification of archive-quality DNA from cerebrospinal fluid (CSF) using Gentra Puregene Kits? FAQ ID -1967 View Do you have a protocol for the purification of archive-quality DNA from Chlamydomonas using Gentra Puregene Kits? FAQ ID -1968 View Do you have a protocol for the purification of archive-quality DNA from blood smears using Gentra Puregene Kits? FAQ ID -1948 View Do you have a protocol for the purification of archive-quality DNA from fecal cells using Gentra Puregene Kits? FAQ ID -1972 View Do you have a protocol for the purification of archive-quality DNA from buccal brushes using Gentra Puregene Kits? FAQ ID -1965 View Do you have a protocol for the purification of archive-quality DNA from fish using Gentra Puregene Kits? FAQ ID -1973 View Do you have a protocol for the purification of archive-quality DNA from Eimeria oocysts using Gentra Puregene Kits? FAQ ID -1952 View Do you have a protocol for the purification of archive-quality DNA from fungus using Gentra Puregene Kits? FAQ ID -1971 View Do you have a protocol for cleanup of REPLI-g amplified DNA? FAQ ID -1545 View What are the Tms of PCR products obtained using the miScript System? FAQ ID -1599 View What is Q-Bond used in the QIAGEN Fast Cycling PCR and QuantiFast Kits? FAQ ID -1554 View What controls should be used for normalizing miRNA real-time PCR data obtained with the miScript System? FAQ ID -1596 View Will QuantiTect Primer Assays work at an annealing temperature of 60ºC with QuantiFast SYBR Green PCR and RT-PCR Kits? FAQ ID -1553 View Which homogenization method do you recommend for tissues stored in Allprotect Tissue Reagent? FAQ ID -1572 View Can different incubation times be used for the 2 hour amplification step in the QuantiTect Whole Transcriptome protocol? -8 View Does residual Allprotect Tissue Reagent left behind on tissue samples interfere with DNA, RNA, and protein purification? FAQ ID -1573 View Is cDNA generated with the QuantiTect Whole Transcriptome Kit suitable for use in microarray analysis? FAQ ID -1587 View Do you have a protocol for purification of genomic DNA from cultured cells using the QIAamp DNA Micro Kit? FAQ ID -1547 View Do you have a protocol for concentration of RNA in 96-well plates? FAQ ID -1546 View Do you have a protocol for purification of total RNA from fatty tissues using QIAzol Lysis Reagent and MaXtract High Density? FAQ ID -1550 View Which real-time PCR kits are recommended downstream of the QuantiTect Whole Transcriptome Kit? FAQ ID -1592 View What primers are used in the reverse-transcription step of the QuantiTect Whole Transcriptome procedure? FAQ ID -1584 View Can the AllPrep DNA/RNA/Protein Mini Kit be used with fibrous or lipid tissues? FAQ ID -1579 View What is the maximum amount of RNA that can be used for amplification with the QuantiTect Whole Transcriptome Kit? FAQ ID -1591 View What starting amount of RNA purified using an miRNeasy Kit should be used with the miScript System? FAQ ID -1597 View Can RNA purified from formalin-fixed, paraffin-embedded (FFPE) tissue be used with the QuantiTect Whole Transcriptome Kit? FAQ ID -1583 View Can samples in Allprotect Tissue Reagent undergo repeated freeze-thaws without degradation of DNA, RNA, and protein? FAQ ID -1575 View Can the QuantiFast Probe PCR +ROX Vial Kit be used on the ABI PRISM 7900 and Applied Biosystems 7500 cyclers? FAQ ID -1555 View Does Buffer APP in the AllPrep DNA/RNA/Protein Mini Kit contain acetone? FAQ ID -1576 View Does cDNA amplified with the QuantiTect Whole Transcriptome Kit correspond to full-length RNA transcripts? FAQ ID -1585 View Can tissue incubated in Allprotect Tissue Reagent be used for morphological studies? FAQ ID -1571 View Can less than 10 ng RNA be used with the QuantiTect Whole Transcriptome Kit? FAQ ID -1590 View Can cDNA prepared by any method be used as starting material in the ligation reaction of the QuantiTect Whole Transcriptome Kit? FAQ ID -1589 View Can Allprotect Tissue Reagent be used to stabilize RNA, DNA and proteins in cells, bacteria, yeast, and plants? FAQ ID -1574 View Can degraded RNA be used with the QuantiTect Whole Transcriptome Kit? FAQ ID -1586 View How much cDNA is sufficient for real-time PCR detection of miRNA transcripts generated with the miScript System? FAQ ID -1598 View How many samples can be run in parallel with the Rotor-Gene Q real-time PCR instrument? FAQ ID -1519 View What main input voltage is required for the QIAxcel Analyzer? FAQ ID -1821 View Is there a quality difference between CompactPrep Plasmid Mega and Giga and the CompactPrep Plasmid Midi and Maxi Kits? FAQ ID -1882 View The Gel Cartridge is installed and all status indicators for the QIAxcel System are good but when clicking the "RUN" button, nothing happens. The green highlight goes down the Sequence? FAQ ID -1850 View Why does the copy function not apply relative to migration time and normalized area values after loading the DNA size marker table and adding the alignment marker peaks on the QIAxcel System? FAQ ID -1832 View Can customers use their own DNA size marker with the QIAxcel System and if yes, what concentration should be used? FAQ ID -1841 View Can data be saved at once with the QIAxcel System after analysis of all samples in a 96-well PCR plate? FAQ ID -1861 View How can a positive threshold be set when running the QIAxcel System? FAQ ID -1843 View How long will the intensity calibration procedure on the QIAxcel System take? FAQ ID -1826 View Will the QIAxcel System run using a green Data Review Software Key? FAQ ID -1853 View When should an intensity calibration be re-performed on the QIAxcel System? FAQ ID -1825 View Can the QIAxcel System or QIAxcel Advanced be connected to a network? FAQ ID -1862 View How can a clogged capillary channel be identified when running samples on the QIAxcel System or QIAxcel Advanced? FAQ ID -1836 View Can the QIAxcel software be loaded to another PC to view the data generated with the QIAxcel System? FAQ ID -1855 View Can multiple QIAxcel Systems be run from one computer? FAQ ID -1860 View How often should the solutions in the Buffer Tray for the QIAxcel System or QIAxcel Advanced be replaced? FAQ ID -1840 View How often should the alignment marker for the QIAxcel System be changed? FAQ ID -1839 View If the M500 method is used with the QIAxcel System, does a new DNA reference marker table have to be created? FAQ ID -1856 View In the results table generated with the QIAxcel System, what does "normalized area" mean? FAQ ID -1854 View What is the composition of buffer OCD? FAQ ID -423 View What is the composition of buffer QN? FAQ ID -414 View What is the composition of buffer OCL? FAQ ID -422 View What is the composition of buffer OL2? FAQ ID -424 View Can the QIAamp MinElute Virus Kits be used to extract viral nucleic acid from cell culture supernatant? FAQ ID -472 View Can RNAi experiments be performed in Drosophila? FAQ ID -436 View How is the RNeasy Lipid Tissue Mini Kit different from the RNeasy Mini Kit? FAQ ID -468 View Has RNAi been successful using siRNA in Zebrafish and Xenopus? FAQ ID -400 View Are Northern Blots sensitive enough to detect siRNA-induced gene silencing? FAQ ID -403 View Can I use the RNeasy MinElute Cleanup Kit to clean up my in vitro transcription reaction? FAQ ID -429 View What is the composition of buffer OEB? FAQ ID -420 View Why is the pQE DNA provided in QIAexpress Kits blue in color? FAQ ID -487 View What is the minimum elution volume when using the RNeasy Micro Kit? FAQ ID -428 View What is the maximum amount of starting material that can be processed with the RNeasy Micro Kit? FAQ ID -426 View What is the minimum elution volume when using the RNeasy MinElute Cleanup Kit? FAQ ID -432 View What is the smallest sample size that can be used with RNeasy Mini Kits? FAQ ID -485 View What is a homogeneous LiquiChip assay? FAQ ID -476 View What detection reagents (reporter fluorophores) do you recommend for the LiquiChip System? FAQ ID -480 View What are the excitation and emission wavelengths of the reporter and classification lasers of the LiquiChip Instrument? FAQ ID -479 View What is the binding capacity of the RNeasy MinElute Cleanup Kit column? FAQ ID -430 View What is the composition and size of the LiquiChip Beads? FAQ ID -475 View What is the binding capacity of the RNeasy Micro Kit column? FAQ ID -427 View Is dihydrofolate reductase (DHFR) immunogenic? FAQ ID -471 View What are some of the assays that are adaptable to the LiquiChip System? FAQ ID -477 View My QuantiTect Master Mix did not freeze at degrees. Is it still ok to use? -20 View I want to start with gene silencing (RNAi) experiments. Do you have informational literature? FAQ ID -580 View Is it possible to use less than 1 mg of protein with the PhosphoProtein Purification Kit? FAQ ID -599 View Is it possible to use a QIAzol lysate for the isolation of RNA from tissue on the BioRobot EZ1? FAQ ID -573 View What is a quenched FRET assay? FAQ ID -542 View What do you recommend for isolating RNA from more than 100 mg of plant tissue? FAQ ID -570 View What happens if I spin my lysate on the RNeasy Spin Columns at maximum speed? FAQ ID -514 View How should I dissolve the lyophilized Anti-His and Tag Antibodies? -100 View Is it possible to perform a UNG treatment when using QuantiTect kits? FAQ ID -564 View Is it possible to clean up a methylation reaction containing bisulfite with QIAquick Cleanup Kits? FAQ ID -519 View Is it possible to isolate both RNA and recombinant 6xHis-tagged protein from the same sample? FAQ ID -532 View Why are my realtime PCR amplification plots hook-shaped? FAQ ID -587 View Why are samples centrifuged at 4°C after the addition of chloroform when using RNeasy Lipid Tissue Kits? FAQ ID -533 View How was it determined that the PhosphoSerine Antibody will bind phosphoserines only? FAQ ID -579 View If pQE40 is used to express a fusion protein with DHFR, can the DHFR be cleaved afterwards? FAQ ID -513 View Which chloroform should I use for the RNeasy Lipid Tissue Kit? FAQ ID -516 View Will pancreas tissue swim on top of RNAprotect Tissue Reagent? FAQ ID -536 View Will the 2x QIAGEN Multiplex PCR Master Mix freeze at -20°C? FAQ ID - 525 View Why does my realtime PCR assay quality decrease over time? FAQ ID -589 View What is the longest fragment that was cloned with the pQE UA Cloning Vector? -30 View What is the difference between BSA-free and BSA-containing RGS-His Antibody? FAQ ID -569 View When using QIAzol Lysis Reagent for RNA extraction, I see a pinkish colored aqueous phase! Is it OK? FAQ ID -534 View Where can I find the public Smith-Waterman homology search tool that you refer to on your siRNA online design page? FAQ ID -562 View What reaction volume should I use with QuantiTect Kits? FAQ ID -551 View What makes QIAGEN's 10x Taq and HotStarTaq DNA Polymerase PCR buffer superior? FAQ ID -566 View How many template copies can I detect using QuantiTect Kits in real-time PCR? FAQ ID -543 View Can I use RNAiFect for co-transfection of plasmid DNA and siRNA? FAQ ID -515 View Does DEPC harm RNA? FAQ ID -529 View Does endogenously expressed DAPase in human cells degrade proteins that are expressed in cell culture? FAQ ID -527 View Can the MAPK1 control siRNA be used for rat cells? FAQ ID -547 View Are the columns of the MinElute Reaction Cleanup-, Gel Extraction-, and PCR Purification Kit identical? FAQ ID -581 View After using your U/A PCR Cloning Kit I can detect colonies, which are light blue, and not really white. Why is that? FAQ ID -512 View Can I make a master mix with QuantiProbes, primers, and all other reaction components and store it for later use? FAQ ID -584 View Can I homogenize samples in QIAzol using the QIAshredder? FAQ ID -501 View Can I shorten the activation time for the HotStarTaq DNA Polymerase? FAQ ID -565 View Does QIAGEN sell 6xHis-tagged proteins besides the 6xHis Protein Ladder? FAQ ID -594 View Do you offer QIAshredder spin columns for the RNeasy Midi/Maxi Kit format? If not, what is the alternative? FAQ ID -560 View Do you provide control proteins for use with the PhosphoProtein Purification Kit? FAQ ID -596 View How long should the amplicon be if I am using QuantiTect SYBR Green detection? FAQ ID -553 View How is an antibody epitope determined? FAQ ID -593 View Do I need to calibrate my real-time cycler if I want to use Yakima Yellow? FAQ ID -540 View Do we recommend pooling siRNA sequences? FAQ ID -517 View Does the His-tag interfere with the purification procedure of the QIAGEN PhosphoProtein Purification Kit? FAQ ID -598 View Does the use of MAPK1 as a control in siRNA transfection experiments have secondary effects on the cell culture? FAQ ID -548 View Do you have a protocol to isolate RNA from blood and bone marrow using the BioRobot EZ1? FAQ ID -567 View Do you have any recommendations for using the PosphoProtein Kit with starting material other than cells? FAQ ID -597 View Do you have a protocol for isolating RNA from yeast using RNeasy? FAQ ID -535 View What is the run time for the BioSprint 96 DNA Blood protocol on the BioSprint 96 instrument? FAQ ID -622 View Will REPLI-g work at high temperatures? FAQ ID -656 View The Multiflow Dispense Head on my BioRobot is not dispensing the correct amount of liquid. What can I do? FAQ ID -652 View What is the smallest amount of DNA I can put into a 50 ul REPLI-g reaction? FAQ ID -663 View What is the recommended incubation time to stabilize tissue RNA in RNAprotect Tissue Reagent? FAQ ID -647 View What do you mean by “Quantification of locus representation” in REPLI-g WGA product? FAQ ID -698 View What is REPLI-g whole genome amplification? FAQ ID -654 View What is the average amount of DNA and RNA present in 1 ml normal serum? FAQ ID -635 View What is the biggest protein that can be synthesized with the EasyXpress Protein Synthesis System? FAQ ID -601 View What is the length of the MDA whole genome amplification product? FAQ ID -690 View What is the expected yield of genomic DNA isolated from bacteria with the DNeasy Blood & Tissue or QIAamp DNA Mini Kit? FAQ ID -632 View Is it possible to perform laser microdissection with tissues stabilized with RNAprotect Tissue reagent? FAQ ID -608 View Is it possible to synthesize membrane proteins using the EasyXpress Protein Synthesis Kit? FAQ ID -602 View What are Elution Microtube adapters and Microplate adapters used in the DirectPrep 96 Miniprep Kit, and how can I order them? FAQ ID -646 View What are the advantages of REPLI-g over conventional DNA sample processing and amplification methods? FAQ ID -686 View Why do I get amplification in a negative control DNA tube using the REPLI-g Kit for WGA? FAQ ID -675 View Why did you choose MAPK1 as a positive control in the QIAGEN RNAi Human/Mouse Control Kit? FAQ ID -604 View Will the PhosphoSerine antibody recognize Serine residues if the neighboring residues are Leucine or Isoleucine? FAQ ID -644 View Will TA cloning work with REPLI-g WGA product? FAQ ID -672 View Will DNA from chromosomes collected in TE with a drop of mineral oil work with REPLI-g amplification? FAQ ID -664 View What is the stability of the REPLI-g MDA product? FAQ ID -693 View When delivering liquid to a 96-well plate using the MultiFlow Dispenser Head on my BioRobot , one or more rows in the plate contains less liquid per well than specified by the protocol. What is going wrong? FAQ ID -653 View Where should I add a dye-label modification on the siRNA? FAQ ID -648 View What types of materials from environmental samples could interfere with WGA? FAQ ID -666 View Can I stabilize RNA in bacteria grown on solid media or substrate using the RNAprotect Bacteria Reagent? FAQ ID -615 View Can I re-amplify whole genome amplification product? FAQ ID -670 View Can I use another lysis buffer than the one supplied in the PhophoProtein Purification Kit? FAQ ID -645 View Can I run less than 15 samples on the BioSprint 15 instrument? FAQ ID -621 View Can the QIAshredder Maxi Spin Columns from the DNeasy Plant Maxi Kit be used for RNeasy samples? FAQ ID -616 View Do you have an off-the shelf diagnostics kit for WGA? FAQ ID -678 View Are there components in biological samples that can inhibit the REPLI-g reaction? FAQ ID -667 View Are the BioSprint 15/96 instruments stand-alone workstations? FAQ ID -620 View Can the computer for the LiquiChip Workstation be networked? FAQ ID -629 View Are QIAGEN EZ Competent Cells of the QIAGEN PCR Cloning Kit dam+ or dam-? FAQ ID -638 View Are samples that are contaminated with high molecular weight DNA (e.g. from a paramedic or police officer) a problem with REPLI-g amplification? FAQ ID -683 View Can BAC DNA prepared with the R.E.A.L. Prep 96 Plasmid Kit be used for high-throughput sequencing? FAQ ID -643 View Can I do whole genome amplification from brain tissue using REPLI-g? FAQ ID -669 View Can I do whole genome amplification from mitochondrial DNA using REPLI-g? FAQ ID -668 View Can FTA paper for dried blood spots be used with REPLI-g? FAQ ID -685 View How can I quantify the amount of REPLI-g DNA I have amplified? FAQ ID -694 View Can I amplify from plasma or serum samples using the REPLI-g Mini/Midi kits? FAQ ID -671 View How should I store the REPLI-g amplification product? FAQ ID -692 View Is 2 kb the minimal gDNA fragment size for REPLI-g Whole Genome Amplification? FAQ ID -682 View How long can I store bacterial culture stabilized in RNAprotect Bacteria Reagent? FAQ ID -614 View Do I need to clean up the REPLI-g amplification product for downstream analysis? FAQ ID -691 View Do you have a kit for RNA isolation from any kind of sample type? FAQ ID -627 View Does REPLI-g technology for whole genome amplification work with paraffin embedded samples? FAQ ID -673 View How can I determine the quality of my REPLI-g amplified products? FAQ ID -695 View How can I decrease ribosomal RNA (rRNA) contamination using Oligotex? FAQ ID -642 View Do you know of anything in the buffers/DNA sample that can cause bias in REPLI-g WGA? FAQ ID -684 View Do you have a protocol for the purification of DNA from rodent tail using the BioSprint System? FAQ ID -979 View Do you have a protocol for the isolation of DNA from epithelial cells mixed with sperm cells? FAQ ID -926 View Do you have a protocol for isolation of genomic DNA from saliva using the DNeasy Blood & Tissue Kit? FAQ ID -931 View Do you have a protocol for purification of DNA from clotted blood using the FlexiGene DNA Kit? FAQ ID -973 View Do you have a protocol for Streptokinase treatment of clotted blood? FAQ ID -912 View Do you have a protocol for the isolation of total nucleic acids from animal and human cells on the BioRobot EZ1? FAQ ID -974 View Do you have a protocol for the isolation of total RNA from plant tissue using QIAGEN-tips? FAQ ID -949 View Do you have a protocol for the isolation of total RNA from buccal swabs? FAQ ID -956 View Do you have a protocol for the isolation of viral DNA from stool? FAQ ID -929 View Do you have a protocol for the isolation of total RNA from plants using the RNeasy 96 Kit? FAQ ID -951 View Do you have a protocol for the isolation of DNA from formalin-preserved stool samples? FAQ ID -922 View Do you have a protocol for the isolation of genomic DNA from fungi? FAQ ID -911 View Do you have a protocol for the isolation of genomic DNA and/or proteins from fatty tissue treated with QIAzol? FAQ ID -955 View Do you have a protocol for the purification of DNA from cells using the BioSprint System? FAQ ID -977 View Do you have a protocol for the purification of DNA from dried blood spots using the BioSprint System? FAQ ID -982 View Do you have a protocol for the preparation of tissues preserved in RNAprotect Tissue Reagent for pathological studies? FAQ ID -950 View Show more results Back to top Contact QIAGEN Global contacts Technical Service Customer Care

How pure should my protein be with the NexTal CubicPhase kits?

FAQ ID -2414

View

How many samples can be processed with the QIAsymphony PAXgene Blood RNA Kit?

FAQ ID -2497

View

How many samples can be processed with the PAXgene Blood RNA MDx Kit?

FAQ ID -2490

View

In the REST software, what is the range indicated by 'Std. Error' on the 'Results' page?

FAQ ID -2455

View

How many PAXgene Blood RNA Tubes can be processed per PAXgene 96 Blood RNA Kit well plate?

FAQ ID -2485

View

If less than 48/96 samples are being processed, is it possible to leave positions empty with the PAXgene Blood RNA MDx Kit?

FAQ ID -2492

View

How does the DNA Protection work in the EpitTect Bisulfite kits?

FAQ ID -2410

View

How is the hypothesis test performed in the REST software?

FAQ ID -2459

View

How do I determine if my gene expression results are statistically significant in the REST software?

FAQ ID -2458

View

How many different genes and gene mutations are on a Somatic Mutation PCR Array?

FAQ ID -2427

View

How long does a QuantiFast Pathogen + IC run take on the Rotor-Gene Q?

FAQ ID -2452

View

How do I know in which phase of my NeXtal CubicPhase cristallisation experiment I am?

FAQ ID -2421

View

How great is the enrichment of miRNA when using the dedicated PAXgene Blood miRNA Kit compared to the PAXgene Blood RNA Kit?

FAQ ID -2495

View

How do I harvest the crystals from meso phase? Are special tools needed?

FAQ ID -2422

View

Why are two reagents used in the PAXgene Tissue System?

FAQ ID -3022

View

Have primer sequences in the Investigator IDplex GO! Kit been changed compared to the Investigator IDplex Plus assay?

FAQ ID -3092

View

Is it possible to increase the amount of sample to obtain higher signals using the Investigator IDplex GO! Kit?

FAQ ID -3090

View

Do you recommend using indicator cards for buccal cells on FTA with Investigator IDplex GO! Kit?

FAQ ID -3095

View

Can the Investigator IDplex GO! PCR protocol be run on different PCR endpoint cyclers?

FAQ ID -3089

View

Do I need to evaluate optimal PCR cycle numbers for the Investigator IDplex GO! Kit with my samples?

FAQ ID -3091

View

Can additional positive controls be used for the Investigator IDplex GO! Kit?

FAQ ID -3094

View

Can the allelic ladder of the Investigator IDplex Plus Kit be used for analysis of Investigator IDplex GO! Kit?

FAQ ID -3093

View

What is the maximum size of tissue that can be fixed in a PAXgene Tissue FIX Container (50 ml)?

FAQ ID -3024

View

5. What is the maximum size of the tissue sample that can be fixed in a PAXgene Tissue Container?

FAQ ID -3023

View

What happens if a tissue sample larger than 20 x 20 x 20 mm is used for fixation with the PAXgene Tissue FIX Container (50 ml)?

FAQ ID -3025

View

How long is the fixation time when using PAXgene Tissue FIX Container?

FAQ ID -3026

View

Is it possible to fix over a weekend?

FAQ ID -3027

View

How long can a tissue specimen stay in the PAXgene Tissue STABILIZER?

FAQ ID -3028

View

What is the minimum incubation time required when using the PAXgene Tissue STABILIZER?

FAQ ID -3029

View

Is it possible to integrate the PAXgene Tissue STABILIZER into the processing?

FAQ ID -3037

View

Is it possible to archive PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?

FAQ ID -3038

View

Is the morphology after H&E staining comparable to formalin-fixed samples?

FAQ ID -3039

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Are immunohistochemistry (IHC) assays developed for formalin-fixed, paraffin-embedded (FFPE) compatible with PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?

FAQ ID -3040

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Is it possible to use formalin-fixed, paraffin-embedded (FFPE) kits and protocols for extraction of biomolecules from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?

FAQ ID -3043

View

is the purity of nucleic acids isolated with the PAXgene Tissue Kits?

FAQ ID -3044

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What is the integrity of RNA from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?

FAQ ID -3046

View

What is the average RNA yield from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?

FAQ ID -3045

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What is the RT-PCR performance of RNA purified from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue compared to RNA from snap frozen or formalin-fixed, paraffin-embedded (FFPE) tissue?

FAQ ID -3050

View

What is the PCR performance of DNA purified from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue compared to DNA from snap frozen or formalin-fixed, paraffin-embedded (FFPE) tissue?

FAQ ID -3051

View

How can proteins purified from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue be analyzed?

FAQ ID -3052

View

What is the DNA integrity from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?

FAQ ID -3047

View

Does the PAXgene Tissue RNA Kit co-purify small RNAs?

FAQ ID -3048

View

How can I extract proteins from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?

FAQ ID -3049

View

Can PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue be used for in situ hybridization?

FAQ ID -3042

View

What are the typical yields obtained from Buccal swabs on BioSprint 96?

FAQ ID -3071

View

Can I use a USB drive to transfer protocols to BioSprint?

FAQ ID -3072

View

Is the Fast Reaction Mix ready to use in the Investigator IDplex GO?

FAQ ID -3087

View

Is it possible to manually process PAXgene Tissue treated samples without using an automated processor?

FAQ ID -3036

View

Are PAXgene Tissue Containers suitable for long term-storage at freezing temperatures?

FAQ ID -3030

View

Is it possible to process formalin-fixed and PAXgene Tissue fixed samples together in one run?

FAQ ID -3033

View

Is it necessary to clean a processor normally used for formalin-fixed tissue before using it with PAXgene Tissue fixed tissue?

FAQ ID -3034

View

Is a special processing protocol needed?

FAQ ID -3035

View

Can sections of PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue be used for other histochemical staining techniques, such as PAS?

FAQ ID -3041

View

Are the multicopy reference assays for Type-it CNV PCR +qC Kits and the qBiomarker assays the same?

FAQ ID -3000

View

How do I clean up RNA preparations containing miRNA?

FAQ ID -3002

View

What is the fixation method used in the PAXgene Tissue System?

FAQ ID -3019

View

What does PAXgene Tissue FIX contain?

FAQ ID -3020

View

Can I perform the IDH 1/2 RGQ PCR test with slides containing less than 40% tumor cells?

FAQ ID -3098

View

Can the MaXtract High Density Tube be used with QIAzol to isolate RNA?

FAQ ID -1304

View

What volume of bacterial culture can be processed using the QuickLyse Miniprep Kit?

FAQ ID -1357

View

What factors determine if denatured proteins accumulate underneath, or inside the gel of the MaXtract Low and High Density Tubes?

FAQ ID -1312

View

I have a Human Druggable Genome siRNA Set V2.0. Can I reorder the siRNAs from this set?

FAQ ID -1366

View

If both MaXtract Low Density and High Density is suitable for an application, which type is preferable to use?

FAQ ID -1310

View

How will service work carried out on my QIAGEN BioRobot be documented?

FAQ ID -1331

View

If I cannot order FlexiPlate siRNA online, how do I order?

FAQ ID -1371

View

What information about my gene or siRNA do I need to order FlexiPlate siRNA?

FAQ ID -1365

View

Is it possible to use MaXtract for an organic extraction with a mixture of phenol and BCP (1 bromine chlorpropane)?

-3

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What information is provided with FlexiPlate siRNAs?

FAQ ID -1363

View

Is it possible to elute plasmid DNA obtained with the QuickLyse Miniprep Kit in nuclease-free water?

FAQ ID -1355

View

Which formats are available for FlexiPlate siRNA?

FAQ ID -1358

View

When isolating DNA from plant cells using CTAB, should MaXtract Low or High Density be used?

FAQ ID -1308

View

Which parts are exchanged during a Preventive Maintenance visit for my QIAGEN BioRobot?

FAQ ID -1334

View

Which controls can I choose for FlexiPlate siRNA?

FAQ ID -1368

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Which density gel type is contained in the yellow or green MaXtract Tubes?

FAQ ID -1315

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What is the composition of FlexiPlate siRNA buffer?

FAQ ID -1361

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Which software updates are included in a Premium Cover Agreement for my QIAGEN Instrument?

FAQ ID -1336

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Can MaXtract tubes be autoclaved prior to use?

FAQ ID -1305

View

Can I reduce the amount of starting material below 10 ng when using the REPLI-g UltraFast Mini Kit?

FAQ ID -1325

View

Can I reduce the reaction time to e.g., 30 minutes, when using the REPLI-g UltraFast Mini Kit?

FAQ ID -1326

View

Can I use any type of DNA extraction method to purify template DNA for amplification using the REPLI-g Mitochondrial DNA Kit?

FAQ ID -1321

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Can several extraction steps be performed in the same MaXtract tube?

FAQ ID -1302

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Can a phenol-chloroform ratio different from 1:1 also be used with MaXtract Low and High Density?

FAQ ID -1309

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Can DNA isolated with MaXtract tubes be used for downstream applications such as restriction digestion, labeling, blotting, PCR, and automated sequencing?

FAQ ID -1303

View

Can I amplify mitochondrial DNA directly from whole blood or cells using the REPLI-g Mitochondrial DNA Kit?

FAQ ID -1319

View

Can I order Alexa Fluor labeled controls with FlexiPlate siRNA?

FAQ ID -1369

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Can HP Validated siRNAs be ordered on the FlexiPlate?

FAQ ID -1367

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Can frozen sample material pulverized in liquid nitrogen be directly added to MaXtract tubes?

FAQ ID -1301

View

Can I also screen large constructs like BACs, fosmids, and cosmids using the QIAGEN PlasmidAmp Kit?

FAQ ID -1337

View

How much REPLI-g amplified mitochondrial DNA should be used in a subsequent PCR reaction?

FAQ ID -1320

View

Do mutations in the target mitochondrial DNA prevent primer binding, resulting in no DNA amplification with the REPLI-g Mitochondrial DNA Kit?

FAQ ID -1323

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Do you have stability data for genomic DNA isolated with the BioSprint Kits?

FAQ ID -1318

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Do you have stability data for genomic DNA isolated with the Flexigene DNA Kit?

FAQ ID -1317

View

Does the addition of sample and organic solution to the MaXtract Tubes require a specific pipetting sequence?

FAQ ID -1307

View

How can I upload my gene list for FlexiPlate siRNA to GeneGlobe?

FAQ ID -1364

View

How does a Service Support Agreement provide defined budgeting for maintaining my BioRobot?

FAQ ID -1330

View

How can I ensure optimal performance of my QIAGEN instrument?

FAQ ID -1329

View

Is it possible to purify viral RNA using FastLane Cell RT-PCR Kits?

FAQ ID -1484

View

Why is the activation time for HotStarTaq Plus Polymerase in the QuantiFast SYBR Green Kits different from that for QuantiFast Probe Kits?

FAQ ID -1449

View

Why is the RT step with the QuantiFast RT Kits much shorter compared to QuantiTect RT Kits?

FAQ ID -1451

View

Why is the storage time for QuantiFast PCR Kits shorter than that for QuantiTect PCR Kits?

FAQ ID -1446

View

Why is the reaction volume for QuantiFast PCR Kits lower than that for QuantiTect PCR Kits?

FAQ ID -1447

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Why is the QuantiFast denaturation step different for PCR and RT-PCR runs in the two-step protocol for the ABI 7500 and other cyclers?

FAQ ID -1442

View

What is the minimum number of siRNAs per order? If I order 40 siRNAs, can I get those on 2 plates?

FAQ ID -1468

View

Can purification columns from other suppliers be processed on the QIAcube?

FAQ ID -1406

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Can QIAcube protocol runs be interrupted, and subsequently be continued to completion?

FAQ ID -1414

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Can I use more than 4 µl FastLane lysate in subsequent real-time RT-PCR?

FAQ ID -1480

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Are FlexiPlate siRNAs available at a higher final concentration than 10 µM?

FAQ ID -1476

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Can different amounts of siRNA be ordered on one FlexiPlate?

FAQ ID -1473

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Can 2 µl reaction volumes be used with QuantiFast PCR Kits?

FAQ ID -1440

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Do the QuantiTect Primer Assays work with QuantiFast SYBR Green Kits?

FAQ ID -1439

View

Do special settings have to be used for QuantiFast PCR Kits on the Eppendorf Mastercycler ep realplex?

FAQ ID -1437

View

What information is required to reorder specific siRNAs from a siRNA Set in FlexiPlate format?

FAQ ID -1463

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What are the turnaround times for FlexiPlate siRNA orders?

FAQ ID -1478

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How much time will be saved when switching from standard cycling to fast cycling with QuantiFast Kits?

FAQ ID -1438

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How many reactions can I perform with the new QuantiFast Kits for real-time PCR?

FAQ ID -1425

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If FlexiPlate siRNA was accidentally redissolved in siRNA Suspension Buffer instead of water, resulting in a 2x buffer, would it affect performance of the siRNAs?

FAQ ID -1477

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How do you achieve fast cycling, yet still deliver the same performance in PCR as that achieved with standard cycling?

FAQ ID -1430

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Is it necessary to place the lids of the elution tube and column into the slots of the rotor adapter during processing on the QIAcube?

FAQ ID -1415

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Why do QuantiFast SYBR Green PCR Kits require such a high primer concentration?

FAQ ID -1443

View

What QuantiFast Kit should be used on the Eppendorf Mastercycler ep realplex?

FAQ ID -1436

View

What is the detection limit of the QuantiFast Kits for real-time PCR?

FAQ ID -1432

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Which kind of 96 and 384-well plates are available for FlexiPlate siRNAs?

FAQ ID -1459

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Which cell lines have been tested with FastLane Cell RT-PCR Kits?

FAQ ID -1485

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Can I adjust the ROX concentration in the QuantiFast master mix?

FAQ ID -1427

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Can I follow the reaction setup for probe-based assays described in Appendix D of the FastLane Cell RT-PCR Handbook using 384-well plates?

FAQ ID -1482

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Can FlexiPlate siRNAs be ordered in plates other than the standard NUNC 96 well V bottom plates?

FAQ ID -1458

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Can fast cycling be carried out with QuantiFast Probe PCR Kits and FRET probes?

FAQ ID -1433

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Can FlexiPlate siRNA be ordered in solution on dry-ice?

FAQ ID -1474

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How do QuantiFast PCR Kits compare to QuantiTect PCR Kits for quantitative real-time PCR?

FAQ ID -1441

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How can I get software updates for the QIAcube?

FAQ ID -1413

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Do you offer trial-kit sizes for the new QuantiFast Kits?

FAQ ID -1429

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Can the QIAcube rotor adapters be autoclaved?

FAQ ID -1409

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Can QuantiFast PCR Kits be used on real-time PCR instruments without fast cycling options?

FAQ ID -1428

View

Does the high primer concentration required by QuantiFast SYBR Green PCR Kits impair annealing specificity?

FAQ ID -1444

View

Does the master mix in the QuantiFast Kits contain dUTP to allow UNG treatments?

FAQ ID -1431

View

Can the QuantiFast Probe PCR +ROX Vial mastermix be stored long-term after adding ROX dye?

FAQ ID -1435

View

What are important points regarding the genomic DNA wipeout buffer in the QuantiFast Probe RT-PCR Plus Kit?

FAQ ID -2359

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Will the sequence of the QuantiFast Probe Assays be provided?

FAQ ID -2369

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Why are samples centrifuged at 4°C after addition of chloroform to the QIAzol lysates in following kits: RNeasy Lipid Tissue Mini/Midi kits, RNeasy Microarray Tissue Mini kit, RNeasy Plus Universal Mini/Midi kit, RNeasy 96 Universal Tissue kit, miRNeasy Mini kit, miRNeasy Micro kit, miRNeasy 96 kit and miRNeasy Serum/Plasma kit?

FAQ ID -2345

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Why are there various different QuantiFast Probe Assays for my gene (previous versions, transcript variants)?

FAQ ID -2366

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Why are the QuantiFast Probe Assays located on a single exon, thereby also detecting genomic DNA?

FAQ ID -2365

View

What are the main differences between the qBiomarker PCR Arrays and the RT2 Profiler PCR Arrays?

FAQ ID -2438

View

What are the recommended storage conditions of the QuantiFast Probe RT-PCR Plus Kit components?

FAQ ID -2355

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What are the differences between the existing QuantiFast Probe RT-PCR Kit and the new Plus Kit?

FAQ ID -2358

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What is the concentration of the primers and the probe in a reaction using the QuantiFast Probe Assays?

FAQ ID -2370

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What is the average length of a QuantiFast Probe Assay?

FAQ ID -2374

View

Is the RNeasy Plus Universal Mini Kit suited for adipose, brain, and other fatty tissues?

FAQ ID -2343

View

Is there a size limit to the protein I can crystallize with the NexTal Cubic Phase products?

FAQ ID -2331

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Is the T790M mutation included in the EGFR PCR kit?

FAQ ID -2314

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Is it necessary to perform calibration steps for the use of the MAX dye in duplex RT-PCR experiments on different cyclers?

FAQ ID -2373

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Is there also a miRNeasy Plus Universal Kit available?

FAQ ID -2346

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I use cultured cells as starting material. Can I also use the RNeasy Plus Universal Kit?

FAQ ID -2342

View

I would like to order the QuantiFast Probe Assays without master mix. Is this possible?

FAQ ID -2360

View

My ADAPT report states "Run Invalid" and does not display any sample results. Why is this?

FAQ ID -2306

View

Is V600E the only mutation that can be detected with the codon 600 assay in the BRAF Pyro Kit?

FAQ ID -2382

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Is the yield and quality of RNA and DNA purified with the AllPrep DNA/RNA FFPE Kit comparable to yield and quality of nucleic acids purified separately with other methods.

FAQ ID -2349

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Which purification kits are recommended for the Pyro kits?

FAQ ID -2380

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Which kit, the QIAprep 96 Plus Miniprep Kit or the QIAGEN Plasmid Plus 96 Miniprep Kit, do you recommend for transfection?

FAQ ID -2320

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Which probe labels are available for the QuantiFast Probe Assays?

FAQ ID -2361

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What should be the concentration of my protein with the NexTal Cubic Phase products?

FAQ ID -2325

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What is the maximum plasmid size which I can use with the QIAprep 96 Plus Miniprep Kit or the QIAGEN Plasmid Plus 96 Miniprep Kit?

FAQ ID -2316

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What plug-ins are available for the Pyrosequencing kits?

FAQ ID -2381

View

Which crystallization solutions are compatible with lipidic cubic phase crystallization?

FAQ ID -2418

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Which deparaffinization method can I use with the AllPrep DNA/RNA FFPE Kit?

FAQ ID -2352

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What species are QuantiTect Probe Assays offered for?

FAQ ID -2367

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Can the insertions in Exon 20 be detected with the EGFR Pyro Kit?

FAQ ID -2383

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Is it also possible to isolate miRNA with the AllPrep DNA/RNA FFPE Kit?

FAQ ID -2348

View

Can I use the RNeasy Plus Universal Mini Kit for all types of tissue?

FAQ ID -2338

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Can the QIAprep 96 Plus Miniprep Kit or the QIAGEN Plasmid Plus 96 Miniprep Kit be used for low-copy number plasmids?

FAQ ID -2317

View

Can the QuantiFast Probe Assay detect short fragmented RNA as well as longer fragments of RNA?

FAQ ID -2364

View

Can negative crossing threshold (CT) values be negative when using the KRAS and EGFR PCR kits?

FAQ ID -2300

View

Can QuantiFast Probe Assays be ordered offline via e-mail or fax?

FAQ ID -2368

View

For the the QIAprep 96 Plus Miniprep Kit or the QIAGEN Plasmid Plus 96 Miniprep Kit, can plates be partially used?

FAQ ID -2319

View

For which applications can the RNA isolated with the RNeasy Plus Universal Kit also be used?

FAQ ID -2339

View

Does the KRAS PCR kit cover the codon 61 mutation?

FAQ ID -2308

View

Are the QIAprep 96 Plus Miniprep Kit or the QIAGEN Plasmid Plus 96 Miniprep Kit suited for short hairpin vectors?

FAQ ID -2318

View

Are the QuantiFast Probe Assays hydrolysis or FRET probes?

FAQ ID -2363

View

Are the buffers in the RNeasy QIAcube kit delivered in QIAcube buffer bottles?

FAQ ID -2334

View

Are QIAshredder spin columns delivered with the RNeasy Mini QIAcube Kit?

FAQ ID -2333

View

Can I do singleplex PCR with the QuantiFast Probe Assays?

FAQ ID -2362

View

Can I use more than 10ng DNA for the PCR reaction for the Pyro kits?

FAQ ID -2379

View

Are the QuantiFast Probe Assays wet-lab validated?

FAQ ID -2375

View

At what temperature can I perform the experiment with the NexTal Cubic Phase kits?

FAQ ID -2323

View

How much can the mixed standard ?Ct values vary in the KRAS PCR kit?

FAQ ID -2307

View

How many samples can be processed per RNeasy Mini QIAcube kit?

FAQ ID -2332

View

How much DNA/RNA can be isolated with the AllPrep DNA/RNA FFPE Kit?

FAQ ID -2353

View

How many freeze-thaw cycles can the master mix contained in the QuantiFast Probe RT-PCR Plus Kit tolerate?

FAQ ID -2357

View

How pure should the chloroform used with the RNeasy Plus Universal Mini Kit be?

FAQ ID -2344

View

How much sample material can I use for DNA/RNA purification with the AllPrep DNA/RNA FFPE Kit?

FAQ ID -2351

View

How much time can be saved when using the RNeasy Mini QIAcube Kit?

FAQ ID -2335

View

How does the QuantiFast Probe RT-PCR Plus Kit eliminate genomic DNA contamination?

FAQ ID -2354

View

How do I analyze the data for the particular iPSC qBiomarker Arrays I have ordered?

FAQ ID -2440

View

How are the genes in each iPSC qBiomarker PCR Array selected?

FAQ ID -2439

View

How can I adjust the ROX concentration in the QuantiFast Probe RT-PCR Plus master mix?

FAQ ID -2356

View

How do I harvest the crystals from meso phase? Are special tools needed with the NexTal Cubic Phase products?

FAQ ID -2330

View

What is the advantage of EpiTect MethyLight PCR Kits over QuantiTect Probe PCR Kits for methylation studies?

FAQ ID -2010

View

What is the advantage of using the Type-it Microsatellite PCR Kit?

FAQ ID -2059

View

What is a good starting amount of template DNA for PCR with the EpiTect MSP Kit?

FAQ ID -2005

View

What is the advantage of using the Type-it Mutation Detect PCR Kit?

FAQ ID -2064

View

What is the maximum lifetime of the QIAGEN rotor for the Centrifuge 4 and 4K?

-16

View

What is the principle behind the DNA Polymerase in the EpiTect MSP Kit?

FAQ ID -2003

View

What is the maximum DNA yield from forensic samples processed with the QIAsymphony SP?

FAQ ID -2028

View

Is it possible to use different sample types in one run with the MagAttract 96 cador Pathogen Kit?

FAQ ID -2072

View

Is magnetic particle carry-over in the eluates on the BioSprint 96 normal?

FAQ ID -2073

View

What happens when using EpiTect MethyLight Assays on only partially bisulfite converted DNA template?

FAQ ID -2009

View

What are the expected PCR results when using EpiTect Control DNA on untreated or bisulfite-converted DNA?

FAQ ID -2011

View

What are the features of the expression vector used for QIAgenes Insect/Mammalia?

FAQ ID -2050

View

Why is CoralLoad included in the Type-it Mutation Detect, but not in the Type-it Microsatellite PCR Kit?

FAQ ID -2068

View

Why do casework-sample protocols for the QIAsymphony DNA Investigator Kit start with different lysate volumes (200 µl, 500 µl, or 1000 µl)?

FAQ ID -2035

View

Why do pQE-T7 vectors of the QIAgenes E. coli system have an additional UAG amber stop codon?

FAQ ID -2043

View

Why is the Type-it Mutation Detect PCR Kit recommended for preamplification of SNPs?

FAQ ID -2067

View

Why is maximum amplicon size for the Type-it Microsatellite PCR Kit limited to 500 bp?

FAQ ID -2060

View

Will the optimized DNA sequence for QIAgenes be provided?

FAQ ID -2045

View

What sample types can be processed using the QIAsymphony Virus/Bacteria protocols?

FAQ ID -2036

View

What sample volumes can be processed with the QIAsymphony Virus/Bacteria Kits?

FAQ ID -2037

View

Can data be analyzed on the Rotor-Gene Q while a run is in progress?

FAQ ID -2091

View

Are there recommendations for sensitive detection of underrepresented fragments using the Type-it Mutation Detect PCR Kit?

FAQ ID -2066

View

Are there two vectors for protein expression using QIAgenes in insect or mammalian cells?

FAQ ID -2048

View

Is mitochondrial DNA isolated with the QIAsymphony DNA Investigator Kit on the QIAsymphony SP?

FAQ ID -2030

View

Is it possible to edit the sample sheet in the Rotor-Gene Q software after a PCR run?

FAQ ID -2092

View

Is it possible to import a standard curve from a previous PCR run on the Rotor-Gene Q?

FAQ ID -2093

View

What kind of samples should be processed using the large-volume protocol with the QIAsymphony DNA Investigator Kit?

FAQ ID -2034

View

Which QIAsymphony Virus/Bacteria Kit size should be used for each protocol?

FAQ ID -2038

View

Which expression systems can be used with the QIAgenes Insect/Mammalia expression constructs?

FAQ ID -2049

View

What types of reaction vessels are required for use in the Rotor-Gene Q?

FAQ ID -2085

View

Which capillary sequencers can be used for detection of amplicons from the Type-it Microsatellite PCR Kits?

FAQ ID -2063

View

Can the EpiTect PCR Control DNAs be used for mouse and rat assays?

FAQ ID -2008

View

Can the EpiTect PCR Control DNAs also be used as standard in real-time methylation specific quantification?

FAQ ID -2006

View

Can the protocols for purification of small RNAs from cells in the appendices of the AllPrep DNA/RNA 96 Handbook also be used with tissue samples?

FAQ ID -2001

View

Can QIAGEN rotors used with the centrifuges 4C and 4KC also be used with 4-16 and 4-16K models?

-15

View

Can the MagAttract 96 cador Pathogen Kit also be used on the KingFisher 96 or MagMAX Express 96 instruments?

FAQ ID -2074

View

Can TopTaq Master Mix simply be stored at room temperature on the lab bench for up to 4 months?

FAQ ID -2052

View

Do all QIAsymphony DNA Investigator protocols use carrier RNA?

FAQ ID -2033

View

Can the Type-it Microsaltellite PCR Kit be used on cyclers with fast ramping rates?

FAQ ID -2062

View

Can the Type-it Mutation Detect PCR kit be used for multiplex PCR of fragments longer than 500 bp?

FAQ ID -2065

View

Can 0.1 ml and 0.2 ml tubes used on the Rotor-Gene Q be labeled?

FAQ ID -2082

View

Can DNase digestion be performed on the RNeasy 96 plate during the RNA purification part of the Allprep DNA/RNA 96 procedure?

FAQ ID -2000

View

How many signal readings are taken during the real-time data acquisition on the Rotor-Gene Q?

FAQ ID -2083

View

Can membrane proteins be expressed with QIAgenes expression kits?

FAQ ID -2047

View

Do QIAsymphony Virus/Bacteria samples require pretreatment before they are loaded onto the instrument?

FAQ ID -2039

View

Does the centrifugal force have any effect on the kinetics of a PCR reaction on the Rotor-Gene Q?

FAQ ID -2081

View

Does an internal control have to be included for each QIAsymphony Virus/Bacteria protocol run?

FAQ ID -2040

View

How can full-length genes or individual domains of the QIAgenes Insect/Mammalia constructs be subcloned?

FAQ ID -2051

View

Can frozen samples be used with the MagAttract 96 cador Pathogen Kit?

FAQ ID -2070

View

How fast does the rotor spin during a run on the Rotor-Gene Q?

FAQ ID -2080

View

How is the log graph threshold value (Ct) for the Rotor-Gene Q determined?

FAQ ID -2088

View

How long can DNA converted with EpiTect Bisulfite Kits be stored before use in methylation specific PCR with the EpiTect MSP Kit?

FAQ ID -2002

View

How will running less than 96 samples with the MagAttract 96 cador Pathogen kit affect usage of plasticware?

FAQ ID -2071

View

If new dyes or chemistries appear on the real-time PCR market, will Rotor-Gene Q be capable of running those dyes?

FAQ ID -2087

View

If orders cannot be placed online, how can QIAgenes be ordered?

FAQ ID -2046

View

How much space does the PyroMark Q24 instrument need?

FAQ ID -2098

View

Does the Type-it Microsatellite PCR Kit always require an annealing temperature of 60°C, or can pre-optimized annealing temperatures for target-primer systems be used?

FAQ ID -2061

View

What is the turn around time for the ordering of miScript Target Protector?

FAQ ID -2254

View

What is the optimal range of PCR fragment sizes for normalization with the SeqTarget System?

FAQ ID -2247

View

What is the processing time for 96 normalizations with the SeqTarget System?

FAQ ID -2237

View

What technologies are used by the KRAS and EGFR PCR kits?

FAQ ID -2286

View

What method do you recommend to quantify DNA before running the KRAS and EGFR PCR kit?

FAQ ID -2292

View

What kind of automation can be done with the EasyXtal 15-well Tool?

FAQ ID -2220

View

What format are the EasyXtal 15-well tools?

FAQ ID -2219

View

What dyes are used in the KRAS and EGFR PCR kits?

FAQ ID -2288

View

What do I do if I get amplification in my no template control (NTC) with the KRAS and EGFR PCR kits?

FAQ ID -2298

View

What do I need to provide when ordering a miScript Target Protector?

FAQ ID -2251

View

What is the difference of the sequence of a miScript miRNA Inhibitor and miScript Target Protector?

FAQ ID -2253

View

What is a miScript Target Protector?

FAQ ID -2249

View

What is a miScript Precursor Assay?

FAQ ID -2214

View

Which transfection reagent is recommended for transfection of the miScript Target Protectors?

FAQ ID -2252

View

Which variants of the EasyXtal 15-well tool are available?

FAQ ID -2221

View

Why are most of my samples outside of the cluster in supervised mode using Rotor-Gene ScreenClust HRM Software?

FAQ ID -2205

View

Why are some of my samples outside of the cluster using Rotor-Gene ScreenClust HRM Software?

FAQ ID -2204

View

Why does the miScript Target Protector not induce the degradation process?

FAQ ID -2263

View

Why does the SeqTarget Prep Protocol require a 2-step procedure?

FAQ ID -2238

View

What will happen, if the normalized DNA for the SeqTarget System is eluted with Buffer EB instead of EB2?

FAQ ID -2242

View

When negative results are generated using the KRAS and EGFR PCR kits, do I rerun them?

FAQ ID -2294

View

What type of samples can I use for the KRAS PCR and EGFR kits?

FAQ ID -2293

View

What voltage should be used to run my agarose gel with the GelPilot Agarose?

FAQ ID -2261

View

Which mode should I use in the Rotor-Gene ScreenClust HRM Software, supervised or unsupervised?

FAQ ID -2202

View

Do the KRAS and EGFR PCR kits contain a passive reference dye?

FAQ ID -2295

View

Does the atypical look of the lyophilized FlexiTube siRNA Premix have a negative impact on performance?

FAQ ID -2267

View

Does the miScript Target Protector match the target sequence?

FAQ ID -2250

View

How do I store my FlexiTube siRNA Premix?

FAQ ID -2270

View

Do I have to order at least 4 FlexiTube siRNA Premixes?

FAQ ID -2266

View

How is ARMS technology superior to alternative methods such as dye terminator sequencing?

FAQ ID -2290

View

How do I search for all the miScript Target Protectors for one species?

FAQ ID -2255

View

How can I check the success of the normalization procedure for the SeqTarget System?

FAQ ID -2245

View

Can PCR fragments be normalized for the SeqTarget System after purification with QIAquick?

FAQ ID -2240

View

Are all 3 components of the SeqTarget System required or can a different Long Range PCR Kit and primers be used?

FAQ ID -2234

View

Can PCR fragments be normalized for the SeqTarget System without a previous purification step?

FAQ ID -2246

View

Can I use a 384 well plate for the KRAS and EGFR PCR kits?

FAQ ID -2296

View

Can the miScript Precursor Assay differentiate between pri- and pre-miRNA?

FAQ ID -2211

View

Does ethanol from the PCR product purification influence the normalization procedure for the SeqTarget System?

FAQ ID -2244

View

What are EasyXtal 15-well Tools used for?

FAQ ID -2217

View

What are Principal Components analyzed in Rotor-Gene ScreenClust HRM Software?

FAQ ID -2200

View

What are the amplicon lengths of the KRAS and EGFR PCR kits?

FAQ ID -2289

View

What are clusters analyzed in the Rotor-Gene ScreenClust HRM Software?

FAQ ID -2201

View

Is the SeqTarget System compatible with standard-size PCRs?

FAQ ID -2241

View

What controls are used in the KRAS and EGFR PCR kits to ensure reliable results?

FAQ ID -2291

View

What does QIAGEN use for a positive control in precursor detection experiments?

FAQ ID -2212

View

What do I do if I cannot see amplification in the FAM channel in my KRAS and EGFR PCR kits?

FAQ ID -2297

View

What are the corresponding QIAGEN names for former Biotage instruments?

FAQ ID -2285

View

What can be the reason for low DNA yields after normalization with the SeqTarget System?

FAQ ID -2239

View

How much buffer should be used for agarose gel electrophoresis?

FAQ ID -2257

View

How long does it take to generate results using the KRAS and EGFR PCR kits?

FAQ ID -2299

View

How should gels be cast with the GelPilot Agarose so that optimal resolution is achieved?

FAQ ID -2258

View

How long should PCR fragments be for the SeqTarget System?

FAQ ID -2236

View

Is it possible to use lower siRNA concentrations with FlexiTube siRNA Premix?

FAQ ID -2264

View

How many miScript Precursor Assays do you offer?

FAQ ID -2213

View

Is one able to use FlexiTube siRNA Premix for transfection of primary adherent cell types?

FAQ ID -2268

View

If I am working with a difficult-to-transfect cell type or if I obtain only weak silencing effects what can I do?

FAQ ID -2265

View

Is one able to use FlexiTube siRNA Premix for transfection of primary suspension cell types?

FAQ ID -2269

View

Is it possible to normalize to more or less than 500 ng with the SeqTarget System if needed?

FAQ ID -2235

View

How many samples can be processed with the QIAsymphony DNA Mini Kit and the Virus Blood 200 protocol?

FAQ ID -2159

View

How often can the Strep-Tactin Superflow Plus resin be reused?

FAQ ID -2169

View

Is it possible to purify and normalize long range PCRs produced with PCR kits from other vendors for the SeqTarget System?

FAQ ID -2243

View

If housekeeping and target genes differ significantly in abundance, will they both be amplified with equal efficiency using the Rotor-Gene Multiplex PCR Kit?

FAQ ID -2129

View

If Vapor-Lock is used to overlay PCR reactions, which volume should be entered as reaction volume in the Rotor-Gene Q Software?

FAQ ID -2150

View

Is it possible to purify a GST-tagged protein under denaturing conditions using Glutathione Superflow?

FAQ ID -2172

View

Is it normal that the binding capacity of GST-resins decreases with flow rate?

FAQ ID -2174

View

Why is the initial activation step different for Rotor-Gene Probe, SYBR Green and Multiplex Kits?

FAQ ID -2118

View

Will Rotor-Gene Kits also work on the Rotor-Gene 6000 and 3000 cyclers?

FAQ ID -2121

View

Why is there an asterisk for my miScript Primer Assays?

FAQ ID -2176

View

Will Uracil-N-Glycosylase (UNG) completely remove contaminating amplicons when using QuantiTect +UNG Kits?

FAQ ID -2139

View

Will QuantiTect Primer Assays work with Rotor-Gene SYBR Green Kits using an annealing step at 60ºC?

FAQ ID -2124

View

What is miRNA*? Do I need to consider this in my profiling experiment?

FAQ ID -2179

View

What are the main differences between Rotor-Gene and QuantiTect or QuantiFast PCR Kits?

FAQ ID -2119

View

What are possible reasons for reduced DNA yields with REPLI-g Kits?

FAQ ID -2148

View

What is the residuals plot in the Rotor-Gene ScreenClust HRM Software?

FAQ ID -2199

View

What is the target nucleic acid for the Virus Blood 200 Protocol on the QIAsymphony?

FAQ ID -2158

View

What is the detection limit of the Rotor-Gene and QuantiFast Multiplex RT-PCR Kits?

FAQ ID -2144

View

What is the minimum number of assays that can be ordered on QuantiTect Primer Assay Plates?

FAQ ID -2109

View

What is the maximum number of targets that can be amplified per reaction with the QuantiFast Multiplex RT-PCR Kit?

FAQ ID -2145

View

How should DNA isolated on the QIAsymphony be stored?

FAQ ID -2165

View

How should samples be stored before purification using the Virus Blood 200 Protocol on the QIAsymphony?

FAQ ID -2157

View

How pure should the chloroform used with the RNeasy Microarray Tissue Mini Kit be?

FAQ ID -2193

View

Why should I use the RNeasy Microarray Tissue Mini Kit when purifying RNA for microarray analysis?

FAQ ID -2188

View

Which elution volumes can be selected using the QIAsymphony Virus Blood 200 protocol?

FAQ ID -2160

View

What sample volumes can be used with the Virus Blood 200 Protocol on the QIAsymphony?

FAQ ID -2156

View

Which is the best condition for eluting GST-tagged proteins from Glutathione Superflow?

FAQ ID -2173

View

What species are QuantiTect Primer Assay Plates offered for?

FAQ ID -2110

View

Which plate formats are offered for QuantiTect Primer Assay Plates?

FAQ ID -2106

View

Why are the denaturation and annealing/extension times for QuantiFast Multiplex RT-PCR Kits much shorter than those for QuantiFast Multiplex PCR Kits?

FAQ ID -2147

View

Which sample types can be processed using the QIAsymphony Virus Blood 200 protocol?

FAQ ID -2154

View

How many reactions can be performed per well of the QuantiTect Primer Assay Plates?

FAQ ID -2107

View

Can assays for 20 and 100 reactions be ordered on the same QuantiTect Primer Assay Plate?

FAQ ID -2108

View

Can QuantiTect Primer Assay Plates be ordered offline via e-mail or fax?

FAQ ID -2112

View

Can Glutathione Superflow resin be reused?

FAQ ID -2171

View

Can Uracil-N-Glycosylase (UNG) be added to the QuantiTect PCR Master Mix and be stored for later use?

FAQ ID -2138

View

Can Uracil-N-Glycosylase (UNG) be purchased separately, independent of QuantiTect PCR Kits?

FAQ ID -2134

View

Are there any changes in the procedure using the Strep-Tactin Superflow Plus resin with the higher capacity over the standard matrix?

FAQ ID -2168

View

Can Uracil-N-Glycosylase (UNG) be used with a one-step RT-PCR kit, such as the QuantiTect Probe RT-PCR Kit?

FAQ ID -2135

View

Can the Rotor-Gene Multiplex RT-PCR Kit be used on other cyclers?

FAQ ID -2142

View

Can RNA isolated with the RNeasy Microarray Tissue Mini Kit also be used in downstream applications other than microarray analysis?

FAQ ID -2191

View

Are QuantiTect PCR +UNG Kits available in bulk format (e.g., 25 ml master mix)?

FAQ ID -2136

View

Are Rotor-Gene Kits compatible with reaction setup using the QIAgility instrument?

FAQ ID -2116

View

How does template quality influence the results with the Type-it HRM PCR Kit?

FAQ ID -2197

View

Are the layouts for both 96- and 384-well QuantiTect Primer Assay Plates visible when ordering online?

FAQ ID -2111

View

Do you have protocols for software setup on various PCR platforms using QuantiFast Probe RT-PCR Kits?

FAQ ID -2105

View

How does magnetic-particle carry-over in QIAsymphony eluates need to be handled?

FAQ ID -2166

View

How does the RNeasy Microarray Tissue Mini Kit prevent traces of phenol from negatively affecting the reverse-transcription step in microarray experiments?

FAQ ID -2189

View

How do Rotor-Gene Probe Kits compare with QuantiFast Probe Kits?

FAQ ID -2125

View

How many elution steps are recommended when using Glutathione Superflow resin?

FAQ ID -2175

View

Do you have a protocol for Rotor-Gene software setup for the Rotor-Gene SYBR Green PCR and RT-PCR Kits?

FAQ ID -2104

View

Does an internal control have to be used with the QIAsymphony Virus Blood 200 Protocol?

FAQ ID -2162

View

Can Vapor-Lock be used with samples processed on the QIAgility?

FAQ ID -2153

View

Do the master mixes in Rotor-Gene Kits contain dUTP to allow UNG pretreatment?

FAQ ID -2117

View

Do QuantiTect Primer Assays also work with Rotor-Gene SYBR Green PCR Kits?

FAQ ID -2123

View

Do limiting primer concentrations need to be determined when using the Rotor-Gene Multiplex PCR Kit?

FAQ ID -2128

View

What buffer should be used to dilute cDNA made using the miScript Reverse Transcription Kit?

FAQ ID -1601

View

Is FlexiTube GeneSolution available for other scales than the 1 nmol scale?

FAQ ID -1666

View

Is it necessary to clean up cDNA prepared with the QuantiTect Whole Transcriptome Kit?

FAQ ID -1610

View

Is the master mix of QuantiFast Kits for real-time PCR aliquoted into several tubes to prevent cross-contamination?

FAQ ID -1697

View

Is the QuantiTect Whole Transcriptome Kit suitable for miRNA amplification?

FAQ ID -1609

View

Is it necessary to include a genomic DNA removal step with the QuantiTect Whole Transcriptome Kit?

FAQ ID -1614

View

Is sequence information for HP Validated siRNAs provided in the FlexiTube format?

FAQ ID -1665

View

Where can I find background information and literature on Whole Genome Amplification with REPLI-g Kits?

FAQ ID -1690

View

Why is there DNA in the no-template (negative) control when using the QuantiTect Whole Transcriptome Kit?

FAQ ID -1620

View

What is the maximum volume of RNA in solution that can be used with the QuantiTect Whole Transcriptome Kit?

FAQ ID -1616

View

Can the Ni-NTA and Strep-Tactin Superflow Cartridges be reused?

FAQ ID -1607

View

Can the Ni-NTA Superflow Cartridges and/or Strep-Tactin Cartridges be connected in series?

FAQ ID -1606

View

Can the QuantiTect Whole Transcriptome Kit be used for amplification of RNA from LCM samples?

FAQ ID -1612

View

Can I store cDNA generated by the miScript System?

FAQ ID -1602

View

Does the QuantiTect Whole Transcriptome Kit work with RNA purified from bacteria, yeast, or plants?

FAQ ID -1615

View

Can T-Script® enzyme of the QuantiTect Whole Transcriptome Kit be substituted by Quantiscript Reverse Transcriptase?

FAQ ID -1617

View

Can the QuantiTect Whole Transcriptome reaction be stopped after the reverse transcription step to proceed with the ligation and amplification steps at a later time?

FAQ ID -1618

View

Can I still reorder siRNA that has been removed from the GeneGlobe data base?

FAQ ID -1668

View

Can amplification with the QuantiTect Whole Transcriptome Kit be extended to more than 8 hours to improve cDNA yields?

FAQ ID -1608

View

Can I select HP Validated and HP GenomeWide siRNAs for FlexiTube?

FAQ ID -1658

View

Can cDNA prepared with the QuantiTect Whole Transcriptome Kit be reampliied using REPLI-g Kits?

FAQ ID -1611

View

Can FlexiTube siRNA or FlexiTube GeneSolution products be ordered in solution?

FAQ ID -1667

View

How fast is the 6xHis-tagged protein purification process using Ni-NTA Superflow Cartridges?

FAQ ID -1605

View

How long is cDNA generated with the QuantiTect Whole Transcriptome Kit?

FAQ ID -1613

View

How long can I archive cDNA generated with the QuantiTect Whole Transcriptome Kit?

FAQ ID -1623

View

Do I need to use the miScript Universal Primer for detection of mRNA?

FAQ ID -1600

View

Have you observed co-amplification of genomic DNA from RNA templates used in the QuantiTect Whole Transcriptome Procedure?

FAQ ID -1619

View

Do you offer a QuantiFast Kit for one-step RT-PCR?

FAQ ID -1695

View

Do you have a protocol for purification of total RNA from plant latex?

FAQ ID -1694

View

Do you have a protocol for purification of total RNA from plant tissue with the RNeasy Lipid Tissue Mini Kit?

FAQ ID -1693

View

What effect does homogenization have on DNA yield and integrity when using AllPrep DNA/RNA Kits?

FAQ ID -1751

View

Is TopTaq DNA Polymerase a hot-start polymerase?

FAQ ID -1748

View

What is the fidelity of TopTaq DNA Polymerase?

FAQ ID -1739

View

Which nucleotide analogs can be used with TopTaq DNA Polymerase?

FAQ ID -1741

View

Why might Affymetrix GeneChip Mapping assays interfere with the REPLI-g FFPE kit?

FAQ ID -1755

View

What is the shelf-life of TopTaq DNA Polymerase and Master Mix?

FAQ ID -1743

View

What is the principle of the REPLI-g FFPE Kit?

FAQ ID -1753

View

What is the largest plasmid size that can be purified using the QuickLyse Miniprep Kit?

FAQ ID -1752

View

What is the minimum amount of starting material that can be used with the RNeasy Plus Micro and AllPrep DNA/RNA Micro Kit?

FAQ ID -1750

View

What is the recommended sample size for use with the REPLI-g FFPE Kit?

FAQ ID -1756

View

Can small miRNA-containing RNA fractions be separated from large RNAs using the miRNeasy FFPE Kit?

FAQ ID -1737

View

Can miRNA and other RNAs smaller than 200 nucleotides be isolated with the AllPrep DNA/RNA Micro Kit or RNeasy Plus Micro Kit?

FAQ ID -1749

View

Can TA/UA cloning be done with PCR products amplified with TopTaq DNA Polmyerase and TopTaq Master Mix Kits?

FAQ ID -1740

View

Are there problems with the default threshold setting on the Applied Biosystems 7500 when using QuantiFast Probe PCR Kits?

FAQ ID -1702

View

At which step in the REPLI-g FFPE protocol should PicoGreen quantification be done?

FAQ ID -1758

View

How can foaming of Buffer RLT Plus lysates be avoided when using RNeasy Plus Micro and Mini Kits, or the Allprep DNA/RNA Mini Kit?

FAQ ID -1734

View

Do you have protocols for sample processing on the Autopure LS instrument?

FAQ ID -1759

View

Have QuantiTect Primer Assays been tested with QuantiFast SYBR Green PCR Kits on the Mastercycler ep realplex?

FAQ ID -1714

View

Can the REPLI-g FFPE procedure restore DNA derived from FFPE tissue samples in the original order?

FAQ ID -1754

View

Can the REPLI-g FFPE Kit also be used for formalin-fixed, ethanol preserved samples?

FAQ ID -1760

View

Do you have a protocol for the isolation of total RNA from paraffin-embedded sections on the Biorobot M48?

FAQ ID -1733

View

Does TopTaq Stabilizer of the TopTaq DNA Polymerase and Master Mix Kits interfere with downstream applications?

FAQ ID -1744

View

Do you have a forensic post-PCR purification protocol to purify double-stranded DNA fragments from PCR reactions?

FAQ ID -1761

View

Is sample pretreatment required for purifying RNA on the QIAsymphony SP?

FAQ ID -1938

View

Is the QIAsymphony RNA Kit compatible with stabilized samples?

FAQ ID -1944

View

Is RNA co-purified with DNA using QIAsymphony DNA Tissue protocols?

FAQ ID -1920

View

Is there a charge for user training on the QIAsymphony SP System?

FAQ ID -1900

View

What are the expected DNA yields from tissue processed on the QIAsymphony SP?

FAQ ID -1919

View

QuantiTect Primer Assays are bioinformatically validated, genomewide primer sets. What does “bioinformatically validated” mean?

FAQ ID -1982

View

What is the range of elution volumes on the QIAsymphony SP?

FAQ ID -1908

View

How many samples can be processed per kit or per reagent cartridge for RNA purification on the QIAsymphony SP?

FAQ ID -1940

View

How does QIAsymphony SP keep cellular samples in suspension prior to pipetting so that samples do not settle?

FAQ ID -1905

View

How many PCR reactions can be performed for different scales of miScript Primer Assays?

FAQ ID -1990

View

How efficient is DNA removal by a gDNA Eliminator 96 plate compared with DNase digestion on an RNeasy 96 plate?

FAQ ID -1994

View

How long can homogenates remain on the QIAsymphony SP without risk of RNA degradation?

FAQ ID -1941

View

How much sample can be used with the Allprep DNA/RNA 96 Kit?

FAQ ID -1997

View

Which types and amounts of sample material can be processed for RNA extraction on the QIAsymphony SP?

FAQ ID -1937

View

How much starting material can be used with the QIAsymphony DNA Mini Kit on the QIAsymphony SP?

FAQ ID -1917

View

What sample types can be used with the AllPrep DNA/RNA 96 Kit?

FAQ ID -1996

View

What sample types should be processed with the High Content (HC) protocol for DNA extraction on the QIAsymphony SP?

FAQ ID -1918

View

What sample types can be used with the RNeasy Plus 96 Kit?

FAQ ID -1991

View

Will mitochondrial DNA also be isolated using the DNA Tissue protocols on the QIAsymphony SP?

FAQ ID -1921

View

Why does the Allprep DNA/RNA 96 Kit use Buffer RLT, whereas the AllPrep DNA/RNA Mini Kit uses Buffer RLT Plus?

FAQ ID -1999

View

Why is there no vacuum-only protocol for the RNeasy Plus 96 Kit?

FAQ ID -1993

View

What is the turn around time for custom miScript miRNA mimics, inhibitors and primer assays?

FAQ ID -1985

View

What kind of molecules are miScript miRNA Inhibitors?

FAQ ID -1983

View

Is it possible to modify QIAsymphony protocols?

FAQ ID -1903

View

What are the expected yields of total RNA isolated with the QIAsymphony SP?

FAQ ID -1942

View

What are the starting volumes of homogenate for RNA extraction on the QIAsymphony SP?

FAQ ID -1939

View

What sample input volumes can be used on the QIAsymphony SP?

FAQ ID -1907

View

What sample types can be processed using the tissue protocols for DNA extraction on the QIAsymphony SP?

FAQ ID -1915

View

What kind of molecules are miScript miRNA Mimics?

FAQ ID -1986

View

What RNA elution volumes are available with the QIAsymphony RNA Kit?

FAQ ID -1943

View

Do limiting primer concentrations need to be determined when using QuantiFast Multiplex PCR Kits?

FAQ ID -1976

View

Do samples require pretreatment before loading onto the QIAsymphony SP for DNA extraction?

FAQ ID -1916

View

Can uneven sample numbers be processed on the QIAsymphony SP module?

FAQ ID -1906

View

Does HiPerFect work for the transfection of miScript miRNA mimics and inhibitors?

FAQ ID -1984

View

Does the QIAsymphony SP system generate run reports and/or log files?

FAQ ID -1911

View

Do you have a protocol for the purification of archive-quality DNA from 100 to 5 x 10e8 cultured cells using Gentra Puregene Kits?

FAQ ID -1960

View

Do you have a protocol for the purification of archive-quality DNA from 0.5–20 mg paraffin-embedded tissue using Gentra Puregene Kits?

FAQ ID -1964

View

Do you have a protocol for the purification of archive-quality DNA from 1 ml of whole blood with the Gentra Puregene Tissue Kit?

FAQ ID -1953

View

Do you have a protocol for rapid purification of archive-quality DNA from up to 9 x 10e6 cells using Gentra Puregene Kits?

FAQ ID -1969

View

Can I program my own protocols for the QIAsymphony SP?

FAQ ID -1904

View

Are QuantiFast Multiplex PCR Kits available in trial sizes?

FAQ ID -1981

View

Can 4-plex, real-time PCR be performed on the ABI PRISM 7000, 7700, or 7900 using the QuantiFast Multiplex PCR Kit?

FAQ ID -1978

View

Can the QIAsymphony RNA Kit be used to purify RNA from blood?

FAQ ID -1945

View

Can the QIAsymphony SP Module be connected to a laboratory information management system (LIMS)?

FAQ ID -1909

View

Can protocol runs on the QIAsymphony SP be interrupted and resumed?

FAQ ID -1912

View

Can the protocols for purification of small RNAs from cells in the appendices of the RNeasy Plus 96 Handbook also be used with tissue samples?

FAQ ID -1995

View

Do you have a protocol for the purification of archive-quality DNA from marine invertebrate tissue using Gentra Puregene Kits?

FAQ ID -1950

View

Do you have a protocol for the purification of archive-quality DNA from nematodes using Gentra Puregene Kits?

FAQ ID -1951

View

Do you have a protocol for the purification of archive-quality DNA from human dried blood using Gentra Puregene Kits?

FAQ ID -1959

View

Do you have a protocol for the purification of archive-quality DNA from Gram-negative bacteria using Gentra Puregene Kits?

FAQ ID -1975

View

Do you have a protocol for the purification of archive-quality DNA from Gram-positive bacteria using Gentra Puregene Kits?

FAQ ID -1946

View

Do you have a protocol for the purification of archive-quality DNA from hair roots using Gentra Puregene Kits?

FAQ ID -1947

View

Do you have a protocol for the purification of DNA from fruit flies?

FAQ ID -1970

View

How can the QIAsymphony SP system be decontaminated?

FAQ ID -1914

View

Do you have a protocol for the purification of archive-quality DNA from tissue fixed in ethanol or formalin using Gentra Puregene Kits?

FAQ ID -1974

View

Do you have a protocol for the purification of archive-quality DNA from umbilical cord blood using Gentra Puregene Kits?

FAQ ID -1958

View

Do you have a protocol for the purification of archive-quality DNA from whole blood using Gentra Puregene Kits?

FAQ ID -1954

View

Do you have a protocol for the purification of archive-quality DNA from buffy coat using Gentra Puregene Kits?

FAQ ID -1955

View

Do you have a protocol for the purification of archive-quality DNA from cerebrospinal fluid (CSF) using Gentra Puregene Kits?

FAQ ID -1967

View

Do you have a protocol for the purification of archive-quality DNA from Chlamydomonas using Gentra Puregene Kits?

FAQ ID -1968

View

Do you have a protocol for the purification of archive-quality DNA from blood smears using Gentra Puregene Kits?

FAQ ID -1948

View

Do you have a protocol for the purification of archive-quality DNA from fecal cells using Gentra Puregene Kits?

FAQ ID -1972

View

Do you have a protocol for the purification of archive-quality DNA from buccal brushes using Gentra Puregene Kits?

FAQ ID -1965

View

Do you have a protocol for the purification of archive-quality DNA from fish using Gentra Puregene Kits?

FAQ ID -1973

View

Do you have a protocol for the purification of archive-quality DNA from Eimeria oocysts using Gentra Puregene Kits?

FAQ ID -1952

View

Do you have a protocol for the purification of archive-quality DNA from fungus using Gentra Puregene Kits?

FAQ ID -1971

View

Do you have a protocol for cleanup of REPLI-g amplified DNA?

FAQ ID -1545

View

What are the Tms of PCR products obtained using the miScript System?

FAQ ID -1599

View

What is Q-Bond used in the QIAGEN Fast Cycling PCR and QuantiFast Kits?

FAQ ID -1554

View

What controls should be used for normalizing miRNA real-time PCR data obtained with the miScript System?

FAQ ID -1596

View

Will QuantiTect Primer Assays work at an annealing temperature of 60ºC with QuantiFast SYBR Green PCR and RT-PCR Kits?

FAQ ID -1553

View

Which homogenization method do you recommend for tissues stored in Allprotect Tissue Reagent?

FAQ ID -1572

View

Can different incubation times be used for the 2 hour amplification step in the QuantiTect Whole Transcriptome protocol?

-8

View

Does residual Allprotect Tissue Reagent left behind on tissue samples interfere with DNA, RNA, and protein purification?

FAQ ID -1573

View

Is cDNA generated with the QuantiTect Whole Transcriptome Kit suitable for use in microarray analysis?

FAQ ID -1587

View

Do you have a protocol for purification of genomic DNA from cultured cells using the QIAamp DNA Micro Kit?

FAQ ID -1547

View

Do you have a protocol for concentration of RNA in 96-well plates?

FAQ ID -1546

View

Do you have a protocol for purification of total RNA from fatty tissues using QIAzol Lysis Reagent and MaXtract High Density?

FAQ ID -1550

View

Which real-time PCR kits are recommended downstream of the QuantiTect Whole Transcriptome Kit?

FAQ ID -1592

View

What primers are used in the reverse-transcription step of the QuantiTect Whole Transcriptome procedure?

FAQ ID -1584

View

Can the AllPrep DNA/RNA/Protein Mini Kit be used with fibrous or lipid tissues?

FAQ ID -1579

View

What is the maximum amount of RNA that can be used for amplification with the QuantiTect Whole Transcriptome Kit?

FAQ ID -1591

View

What starting amount of RNA purified using an miRNeasy Kit should be used with the miScript System?

FAQ ID -1597

View

Can RNA purified from formalin-fixed, paraffin-embedded (FFPE) tissue be used with the QuantiTect Whole Transcriptome Kit?

FAQ ID -1583

View

Can samples in Allprotect Tissue Reagent undergo repeated freeze-thaws without degradation of DNA, RNA, and protein?

FAQ ID -1575

View

Can the QuantiFast Probe PCR +ROX Vial Kit be used on the ABI PRISM 7900 and Applied Biosystems 7500 cyclers?

FAQ ID -1555

View

Does Buffer APP in the AllPrep DNA/RNA/Protein Mini Kit contain acetone?

FAQ ID -1576

View

Does cDNA amplified with the QuantiTect Whole Transcriptome Kit correspond to full-length RNA transcripts?

FAQ ID -1585

View

Can tissue incubated in Allprotect Tissue Reagent be used for morphological studies?

FAQ ID -1571

View

Can less than 10 ng RNA be used with the QuantiTect Whole Transcriptome Kit?

FAQ ID -1590

View

Can cDNA prepared by any method be used as starting material in the ligation reaction of the QuantiTect Whole Transcriptome Kit?

FAQ ID -1589

View

Can Allprotect Tissue Reagent be used to stabilize RNA, DNA and proteins in cells, bacteria, yeast, and plants?

FAQ ID -1574

View

Can degraded RNA be used with the QuantiTect Whole Transcriptome Kit?

FAQ ID -1586

View

How much cDNA is sufficient for real-time PCR detection of miRNA transcripts generated with the miScript System?

FAQ ID -1598

View

How many samples can be run in parallel with the Rotor-Gene Q real-time PCR instrument?

FAQ ID -1519

View

What main input voltage is required for the QIAxcel Analyzer?

FAQ ID -1821

View

Is there a quality difference between CompactPrep Plasmid Mega and Giga and the CompactPrep Plasmid Midi and Maxi Kits?

FAQ ID -1882

View

The Gel Cartridge is installed and all status indicators for the QIAxcel System are good but when clicking the "RUN" button, nothing happens. The green highlight goes down the Sequence?

FAQ ID -1850

View

Why does the copy function not apply relative to migration time and normalized area values after loading the DNA size marker table and adding the alignment marker peaks on the QIAxcel System?

FAQ ID -1832

View

Can customers use their own DNA size marker with the QIAxcel System and if yes, what concentration should be used?

FAQ ID -1841

View

Can data be saved at once with the QIAxcel System after analysis of all samples in a 96-well PCR plate?

FAQ ID -1861

View

How can a positive threshold be set when running the QIAxcel System?

FAQ ID -1843

View

How long will the intensity calibration procedure on the QIAxcel System take?

FAQ ID -1826

View

Will the QIAxcel System run using a green Data Review Software Key?

FAQ ID -1853

View

When should an intensity calibration be re-performed on the QIAxcel System?

FAQ ID -1825

View

Can the QIAxcel System or QIAxcel Advanced be connected to a network?

FAQ ID -1862

View

How can a clogged capillary channel be identified when running samples on the QIAxcel System or QIAxcel Advanced?

FAQ ID -1836

View

Can the QIAxcel software be loaded to another PC to view the data generated with the QIAxcel System?

FAQ ID -1855

View

Can multiple QIAxcel Systems be run from one computer?

FAQ ID -1860

View

How often should the solutions in the Buffer Tray for the QIAxcel System or QIAxcel Advanced be replaced?

FAQ ID -1840

View

How often should the alignment marker for the QIAxcel System be changed?

FAQ ID -1839

View

If the M500 method is used with the QIAxcel System, does a new DNA reference marker table have to be created?

FAQ ID -1856

View

In the results table generated with the QIAxcel System, what does "normalized area" mean?

FAQ ID -1854

View

What is the composition of buffer OCD?

FAQ ID -423

View

What is the composition of buffer QN?

FAQ ID -414

View

What is the composition of buffer OCL?

FAQ ID -422

View

What is the composition of buffer OL2?

FAQ ID -424

View

Can the QIAamp MinElute Virus Kits be used to extract viral nucleic acid from cell culture supernatant?

FAQ ID -472

View

Can RNAi experiments be performed in Drosophila?

FAQ ID -436

View

How is the RNeasy Lipid Tissue Mini Kit different from the RNeasy Mini Kit?

FAQ ID -468

View

Has RNAi been successful using siRNA in Zebrafish and Xenopus?

FAQ ID -400

View

Are Northern Blots sensitive enough to detect siRNA-induced gene silencing?

FAQ ID -403

View

Can I use the RNeasy MinElute Cleanup Kit to clean up my in vitro transcription reaction?

FAQ ID -429

View

What is the composition of buffer OEB?

FAQ ID -420

View

Why is the pQE DNA provided in QIAexpress Kits blue in color?

FAQ ID -487

View

What is the minimum elution volume when using the RNeasy Micro Kit?

FAQ ID -428

View

What is the maximum amount of starting material that can be processed with the RNeasy Micro Kit?

FAQ ID -426

View

What is the minimum elution volume when using the RNeasy MinElute Cleanup Kit?

FAQ ID -432

View

What is the smallest sample size that can be used with RNeasy Mini Kits?

FAQ ID -485

View

What is a homogeneous LiquiChip assay?

FAQ ID -476

View

What detection reagents (reporter fluorophores) do you recommend for the LiquiChip System?

FAQ ID -480

View

What are the excitation and emission wavelengths of the reporter and classification lasers of the LiquiChip Instrument?

FAQ ID -479

View

What is the binding capacity of the RNeasy MinElute Cleanup Kit column?

FAQ ID -430

View

What is the composition and size of the LiquiChip Beads?

FAQ ID -475

View

What is the binding capacity of the RNeasy Micro Kit column?

FAQ ID -427

View

Is dihydrofolate reductase (DHFR) immunogenic?

FAQ ID -471

View

What are some of the assays that are adaptable to the LiquiChip System?

FAQ ID -477

View

My QuantiTect Master Mix did not freeze at degrees. Is it still ok to use?

-20

View

I want to start with gene silencing (RNAi) experiments. Do you have informational literature?

FAQ ID -580

View

Is it possible to use less than 1 mg of protein with the PhosphoProtein Purification Kit?

FAQ ID -599

View

Is it possible to use a QIAzol lysate for the isolation of RNA from tissue on the BioRobot EZ1?

FAQ ID -573

View

What is a quenched FRET assay?

FAQ ID -542

View

What do you recommend for isolating RNA from more than 100 mg of plant tissue?

FAQ ID -570

View

What happens if I spin my lysate on the RNeasy Spin Columns at maximum speed?

FAQ ID -514

View

How should I dissolve the lyophilized Anti-His and Tag Antibodies?

-100

View

Is it possible to perform a UNG treatment when using QuantiTect kits?

FAQ ID -564

View

Is it possible to clean up a methylation reaction containing bisulfite with QIAquick Cleanup Kits?

FAQ ID -519

View

Is it possible to isolate both RNA and recombinant 6xHis-tagged protein from the same sample?

FAQ ID -532

View

Why are my realtime PCR amplification plots hook-shaped?

FAQ ID -587

View

Why are samples centrifuged at 4°C after the addition of chloroform when using RNeasy Lipid Tissue Kits?

FAQ ID -533

View

How was it determined that the PhosphoSerine Antibody will bind phosphoserines only?

FAQ ID -579

View

If pQE40 is used to express a fusion protein with DHFR, can the DHFR be cleaved afterwards?

FAQ ID -513

View

Which chloroform should I use for the RNeasy Lipid Tissue Kit?

FAQ ID -516

View

Will pancreas tissue swim on top of RNAprotect Tissue Reagent?

FAQ ID -536

View

Will the 2x QIAGEN Multiplex PCR Master Mix freeze at -20°C?

FAQ ID - 525

View

Why does my realtime PCR assay quality decrease over time?

FAQ ID -589

View

What is the longest fragment that was cloned with the pQE UA Cloning Vector?

-30

View

What is the difference between BSA-free and BSA-containing RGS-His Antibody?

FAQ ID -569

View

When using QIAzol Lysis Reagent for RNA extraction, I see a pinkish colored aqueous phase! Is it OK?

FAQ ID -534

View

Where can I find the public Smith-Waterman homology search tool that you refer to on your siRNA online design page?

FAQ ID -562

View

What reaction volume should I use with QuantiTect Kits?

FAQ ID -551

View

What makes QIAGEN's 10x Taq and HotStarTaq DNA Polymerase PCR buffer superior?

FAQ ID -566

View

How many template copies can I detect using QuantiTect Kits in real-time PCR?

FAQ ID -543

View

Can I use RNAiFect for co-transfection of plasmid DNA and siRNA?

FAQ ID -515

View

Does DEPC harm RNA?

FAQ ID -529

View

Does endogenously expressed DAPase in human cells degrade proteins that are expressed in cell culture?

FAQ ID -527

View

Can the MAPK1 control siRNA be used for rat cells?

FAQ ID -547

View

Are the columns of the MinElute Reaction Cleanup-, Gel Extraction-, and PCR Purification Kit identical?

FAQ ID -581

View

After using your U/A PCR Cloning Kit I can detect colonies, which are light blue, and not really white. Why is that?

FAQ ID -512

View

Can I make a master mix with QuantiProbes, primers, and all other reaction components and store it for later use?

FAQ ID -584

View

Can I homogenize samples in QIAzol using the QIAshredder?

FAQ ID -501

View

Can I shorten the activation time for the HotStarTaq DNA Polymerase?

FAQ ID -565

View

Does QIAGEN sell 6xHis-tagged proteins besides the 6xHis Protein Ladder?

FAQ ID -594

View

Do you offer QIAshredder spin columns for the RNeasy Midi/Maxi Kit format? If not, what is the alternative?

FAQ ID -560

View

Do you provide control proteins for use with the PhosphoProtein Purification Kit?

FAQ ID -596

View

How long should the amplicon be if I am using QuantiTect SYBR Green detection?

FAQ ID -553

View

How is an antibody epitope determined?

FAQ ID -593

View

Do I need to calibrate my real-time cycler if I want to use Yakima Yellow?

FAQ ID -540

View

Do we recommend pooling siRNA sequences?

FAQ ID -517

View

Does the His-tag interfere with the purification procedure of the QIAGEN PhosphoProtein Purification Kit?

FAQ ID -598

View

Does the use of MAPK1 as a control in siRNA transfection experiments have secondary effects on the cell culture?

FAQ ID -548

View

Do you have a protocol to isolate RNA from blood and bone marrow using the BioRobot EZ1?

FAQ ID -567

View

Do you have any recommendations for using the PosphoProtein Kit with starting material other than cells?

FAQ ID -597

View

Do you have a protocol for isolating RNA from yeast using RNeasy?

FAQ ID -535

View

What is the run time for the BioSprint 96 DNA Blood protocol on the BioSprint 96 instrument?

FAQ ID -622

View

Will REPLI-g work at high temperatures?

FAQ ID -656

View

The Multiflow Dispense Head on my BioRobot is not dispensing the correct amount of liquid. What can I do?

FAQ ID -652

View

What is the smallest amount of DNA I can put into a 50 ul REPLI-g reaction?

FAQ ID -663

View

What is the recommended incubation time to stabilize tissue RNA in RNAprotect Tissue Reagent?

FAQ ID -647

View

What do you mean by “Quantification of locus representation” in REPLI-g WGA product?

FAQ ID -698

View

What is REPLI-g whole genome amplification?

FAQ ID -654

View

What is the average amount of DNA and RNA present in 1 ml normal serum?

FAQ ID -635

View

What is the biggest protein that can be synthesized with the EasyXpress Protein Synthesis System?

FAQ ID -601

View

What is the length of the MDA whole genome amplification product?

FAQ ID -690

View

What is the expected yield of genomic DNA isolated from bacteria with the DNeasy Blood & Tissue or QIAamp DNA Mini Kit?

FAQ ID -632

View

Is it possible to perform laser microdissection with tissues stabilized with RNAprotect Tissue reagent?

FAQ ID -608

View

Is it possible to synthesize membrane proteins using the EasyXpress Protein Synthesis Kit?

FAQ ID -602

View

What are Elution Microtube adapters and Microplate adapters used in the DirectPrep 96 Miniprep Kit, and how can I order them?

FAQ ID -646

View

What are the advantages of REPLI-g over conventional DNA sample processing and amplification methods?

FAQ ID -686

View

Why do I get amplification in a negative control DNA tube using the REPLI-g Kit for WGA?

FAQ ID -675

View

Why did you choose MAPK1 as a positive control in the QIAGEN RNAi Human/Mouse Control Kit?

FAQ ID -604

View

Will the PhosphoSerine antibody recognize Serine residues if the neighboring residues are Leucine or Isoleucine?

FAQ ID -644

View

Will TA cloning work with REPLI-g WGA product?

FAQ ID -672

View

Will DNA from chromosomes collected in TE with a drop of mineral oil work with REPLI-g amplification?

FAQ ID -664

View

What is the stability of the REPLI-g MDA product?

FAQ ID -693

View

When delivering liquid to a 96-well plate using the MultiFlow Dispenser Head on my BioRobot , one or more rows in the plate contains less liquid per well than specified by the protocol. What is going wrong?

FAQ ID -653

View

Where should I add a dye-label modification on the siRNA?

FAQ ID -648

View

What types of materials from environmental samples could interfere with WGA?

FAQ ID -666

View

Can I stabilize RNA in bacteria grown on solid media or substrate using the RNAprotect Bacteria Reagent?

FAQ ID -615

View

Can I re-amplify whole genome amplification product?

FAQ ID -670

View

Can I use another lysis buffer than the one supplied in the PhophoProtein Purification Kit?

FAQ ID -645

View

Can I run less than 15 samples on the BioSprint 15 instrument?

FAQ ID -621

View

Can the QIAshredder Maxi Spin Columns from the DNeasy Plant Maxi Kit be used for RNeasy samples?

FAQ ID -616

View

Do you have an off-the shelf diagnostics kit for WGA?

FAQ ID -678

View

Are there components in biological samples that can inhibit the REPLI-g reaction?

FAQ ID -667

View

Are the BioSprint 15/96 instruments stand-alone workstations?

FAQ ID -620

View

Can the computer for the LiquiChip Workstation be networked?

FAQ ID -629

View

Are QIAGEN EZ Competent Cells of the QIAGEN PCR Cloning Kit dam+ or dam-?

FAQ ID -638

View

Are samples that are contaminated with high molecular weight DNA (e.g. from a paramedic or police officer) a problem with REPLI-g amplification?

FAQ ID -683

View

Can BAC DNA prepared with the R.E.A.L. Prep 96 Plasmid Kit be used for high-throughput sequencing?

FAQ ID -643

View

Can I do whole genome amplification from brain tissue using REPLI-g?

FAQ ID -669

View

Can I do whole genome amplification from mitochondrial DNA using REPLI-g?

FAQ ID -668

View

Can FTA paper for dried blood spots be used with REPLI-g?

FAQ ID -685

View

How can I quantify the amount of REPLI-g DNA I have amplified?

FAQ ID -694

View

Can I amplify from plasma or serum samples using the REPLI-g Mini/Midi kits?

FAQ ID -671

View

How should I store the REPLI-g amplification product?

FAQ ID -692

View

Is 2 kb the minimal gDNA fragment size for REPLI-g Whole Genome Amplification?

FAQ ID -682

View

How long can I store bacterial culture stabilized in RNAprotect Bacteria Reagent?

FAQ ID -614

View

Do I need to clean up the REPLI-g amplification product for downstream analysis?

FAQ ID -691

View

Do you have a kit for RNA isolation from any kind of sample type?

FAQ ID -627

View

Does REPLI-g technology for whole genome amplification work with paraffin embedded samples?

FAQ ID -673

View

How can I determine the quality of my REPLI-g amplified products?

FAQ ID -695

View

How can I decrease ribosomal RNA (rRNA) contamination using Oligotex?

FAQ ID -642

View

Do you know of anything in the buffers/DNA sample that can cause bias in REPLI-g WGA?

FAQ ID -684

View

Do you have a protocol for the purification of DNA from rodent tail using the BioSprint System?

FAQ ID -979

View

Do you have a protocol for the isolation of DNA from epithelial cells mixed with sperm cells?

FAQ ID -926

View

Do you have a protocol for isolation of genomic DNA from saliva using the DNeasy Blood & Tissue Kit?

FAQ ID -931

View

Do you have a protocol for purification of DNA from clotted blood using the FlexiGene DNA Kit?

FAQ ID -973

View

Do you have a protocol for Streptokinase treatment of clotted blood?

FAQ ID -912

View

Do you have a protocol for the isolation of total nucleic acids from animal and human cells on the BioRobot EZ1?

FAQ ID -974

View

Do you have a protocol for the isolation of total RNA from plant tissue using QIAGEN-tips?

FAQ ID -949

View

Do you have a protocol for the isolation of total RNA from buccal swabs?

FAQ ID -956

View

Do you have a protocol for the isolation of viral DNA from stool?

FAQ ID -929

View

Do you have a protocol for the isolation of total RNA from plants using the RNeasy 96 Kit?

FAQ ID -951

View

Do you have a protocol for the isolation of DNA from formalin-preserved stool samples?

FAQ ID -922

View

Do you have a protocol for the isolation of genomic DNA from fungi?

FAQ ID -911

View

Do you have a protocol for the isolation of genomic DNA and/or proteins from fatty tissue treated with QIAzol?

FAQ ID -955

View

Do you have a protocol for the purification of DNA from cells using the BioSprint System?

FAQ ID -977

View

Do you have a protocol for the purification of DNA from dried blood spots using the BioSprint System?

FAQ ID -982

View

Do you have a protocol for the preparation of tissues preserved in RNAprotect Tissue Reagent for pathological studies?

FAQ ID -950

View