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Does TopTaq Stabilizer of the TopTaq DNA Polymerase and Master Mix Kits interfere with downstream applications?
FAQ ID -1744
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Do you have a forensic post-PCR purification protocol to purify double-stranded DNA fragments from PCR reactions?
FAQ ID -1761
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Is sample pretreatment required for purifying RNA on the QIAsymphony SP?
FAQ ID -1938
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Is the QIAsymphony RNA Kit compatible with stabilized samples?
FAQ ID -1944
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Is RNA co-purified with DNA using QIAsymphony DNA Tissue protocols?
FAQ ID -1920
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Is there a charge for user training on the QIAsymphony SP System?
FAQ ID -1900
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What are the expected DNA yields from tissue processed on the QIAsymphony SP?
FAQ ID -1919
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QuantiTect Primer Assays are bioinformatically validated, genomewide primer sets. What does “bioinformatically validated” mean?
FAQ ID -1982
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What is the range of elution volumes on the QIAsymphony SP?
FAQ ID -1908
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How many samples can be processed per kit or per reagent cartridge for RNA purification on the QIAsymphony SP?
FAQ ID -1940
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How does QIAsymphony SP keep cellular samples in suspension prior to pipetting so that samples do not settle?
FAQ ID -1905
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How many PCR reactions can be performed for different scales of miScript Primer Assays?
FAQ ID -1990
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How efficient is DNA removal by a gDNA Eliminator 96 plate compared with DNase digestion on an RNeasy 96 plate?
FAQ ID -1994
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How long can homogenates remain on the QIAsymphony SP without risk of RNA degradation?
FAQ ID -1941
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How much sample can be used with the Allprep DNA/RNA 96 Kit?
FAQ ID -1997
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Which types and amounts of sample material can be processed for RNA extraction on the QIAsymphony SP?
FAQ ID -1937
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How much starting material can be used with the QIAsymphony DNA Mini Kit on the QIAsymphony SP?
FAQ ID -1917
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What sample types can be used with the AllPrep DNA/RNA 96 Kit?
FAQ ID -1996
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What sample types should be processed with the High Content (HC) protocol for DNA extraction on the QIAsymphony SP?
FAQ ID -1918
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What sample types can be used with the RNeasy Plus 96 Kit?
FAQ ID -1991
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Will mitochondrial DNA also be isolated using the DNA Tissue protocols on the QIAsymphony SP?
FAQ ID -1921
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Why does the Allprep DNA/RNA 96 Kit use Buffer RLT, whereas the AllPrep DNA/RNA Mini Kit uses Buffer RLT Plus?
FAQ ID -1999
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Why is there no vacuum-only protocol for the RNeasy Plus 96 Kit?
FAQ ID -1993
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What is the turn around time for custom miScript miRNA mimics, inhibitors and primer assays?
FAQ ID -1985
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What kind of molecules are miScript miRNA Inhibitors?
FAQ ID -1983
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Is it possible to modify QIAsymphony protocols?
FAQ ID -1903
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What are the expected yields of total RNA isolated with the QIAsymphony SP?
FAQ ID -1942
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What are the starting volumes of homogenate for RNA extraction on the QIAsymphony SP?
FAQ ID -1939
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What sample input volumes can be used on the QIAsymphony SP?
FAQ ID -1907
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What sample types can be processed using the tissue protocols for DNA extraction on the QIAsymphony SP?
FAQ ID -1915
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What kind of molecules are miScript miRNA Mimics?
FAQ ID -1986
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What RNA elution volumes are available with the QIAsymphony RNA Kit?
FAQ ID -1943
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Do limiting primer concentrations need to be determined when using QuantiFast Multiplex PCR Kits?
FAQ ID -1976
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Do samples require pretreatment before loading onto the QIAsymphony SP for DNA extraction?
FAQ ID -1916
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Can uneven sample numbers be processed on the QIAsymphony SP module?
FAQ ID -1906
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Does HiPerFect work for the transfection of miScript miRNA mimics and inhibitors?
FAQ ID -1984
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Does the QIAsymphony SP system generate run reports and/or log files?
FAQ ID -1911
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Do you have a protocol for the purification of archive-quality DNA from 100 to 5 x 10e8 cultured cells using Gentra Puregene Kits?
FAQ ID -1960
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Do you have a protocol for the purification of archive-quality DNA from 0.5–20 mg paraffin-embedded tissue using Gentra Puregene Kits?
FAQ ID -1964
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Do you have a protocol for the purification of archive-quality DNA from 1 ml of whole blood with the Gentra Puregene Tissue Kit?
FAQ ID -1953
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Do you have a protocol for rapid purification of archive-quality DNA from up to 9 x 10e6 cells using Gentra Puregene Kits?
FAQ ID -1969
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Can I program my own protocols for the QIAsymphony SP?
FAQ ID -1904
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Are QuantiFast Multiplex PCR Kits available in trial sizes?
FAQ ID -1981
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Can 4-plex, real-time PCR be performed on the ABI PRISM 7000, 7700, or 7900 using the QuantiFast Multiplex PCR Kit?
FAQ ID -1978
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Can the QIAsymphony RNA Kit be used to purify RNA from blood?
FAQ ID -1945
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Can the QIAsymphony SP Module be connected to a laboratory information management system (LIMS)?
FAQ ID -1909
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Can protocol runs on the QIAsymphony SP be interrupted and resumed?
FAQ ID -1912
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Can the protocols for purification of small RNAs from cells in the appendices of the RNeasy Plus 96 Handbook also be used with tissue samples?
FAQ ID -1995
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Do you have a protocol for the purification of archive-quality DNA from marine invertebrate tissue using Gentra Puregene Kits?
FAQ ID -1950
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Do you have a protocol for the purification of archive-quality DNA from nematodes using Gentra Puregene Kits?
FAQ ID -1951
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Do you have a protocol for the purification of archive-quality DNA from human dried blood using Gentra Puregene Kits?
FAQ ID -1959
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Do you have a protocol for the purification of archive-quality DNA from Gram-negative bacteria using Gentra Puregene Kits?
FAQ ID -1975
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Do you have a protocol for the purification of archive-quality DNA from Gram-positive bacteria using Gentra Puregene Kits?
FAQ ID -1946
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Do you have a protocol for the purification of archive-quality DNA from hair roots using Gentra Puregene Kits?
FAQ ID -1947
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Do you have a protocol for the purification of DNA from fruit flies?
FAQ ID -1970
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How can the QIAsymphony SP system be decontaminated?
FAQ ID -1914
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Do you have a protocol for the purification of archive-quality DNA from tissue fixed in ethanol or formalin using Gentra Puregene Kits?
FAQ ID -1974
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Do you have a protocol for the purification of archive-quality DNA from umbilical cord blood using Gentra Puregene Kits?
FAQ ID -1958
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Do you have a protocol for the purification of archive-quality DNA from whole blood using Gentra Puregene Kits?
FAQ ID -1954
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Do you have a protocol for the purification of archive-quality DNA from buffy coat using Gentra Puregene Kits?
FAQ ID -1955
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Do you have a protocol for the purification of archive-quality DNA from cerebrospinal fluid (CSF) using Gentra Puregene Kits?
FAQ ID -1967
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Do you have a protocol for the purification of archive-quality DNA from Chlamydomonas using Gentra Puregene Kits?
FAQ ID -1968
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Do you have a protocol for the purification of archive-quality DNA from blood smears using Gentra Puregene Kits?
FAQ ID -1948
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Do you have a protocol for the purification of archive-quality DNA from fecal cells using Gentra Puregene Kits?
FAQ ID -1972
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Do you have a protocol for the purification of archive-quality DNA from buccal brushes using Gentra Puregene Kits?
FAQ ID -1965
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Do you have a protocol for the purification of archive-quality DNA from fish using Gentra Puregene Kits?
FAQ ID -1973
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Do you have a protocol for the purification of archive-quality DNA from Eimeria oocysts using Gentra Puregene Kits?
FAQ ID -1952
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Do you have a protocol for the purification of archive-quality DNA from fungus using Gentra Puregene Kits?
FAQ ID -1971
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Do you have a protocol for cleanup of REPLI-g amplified DNA?
FAQ ID -1545
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What are the Tms of PCR products obtained using the miScript System?
FAQ ID -1599
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What is Q-Bond used in the QIAGEN Fast Cycling PCR and QuantiFast Kits?
FAQ ID -1554
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What controls should be used for normalizing miRNA real-time PCR data obtained with the miScript System?
FAQ ID -1596
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Will QuantiTect Primer Assays work at an annealing temperature of 60ºC with QuantiFast SYBR Green PCR and RT-PCR Kits?
FAQ ID -1553
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Which homogenization method do you recommend for tissues stored in Allprotect Tissue Reagent?
FAQ ID -1572
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Can different incubation times be used for the 2 hour amplification step in the QuantiTect Whole Transcriptome protocol?
-8
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Does residual Allprotect Tissue Reagent left behind on tissue samples interfere with DNA, RNA, and protein purification?
FAQ ID -1573
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Is cDNA generated with the QuantiTect Whole Transcriptome Kit suitable for use in microarray analysis?
FAQ ID -1587
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Do you have a protocol for purification of genomic DNA from cultured cells using the QIAamp DNA Micro Kit?
FAQ ID -1547
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Do you have a protocol for concentration of RNA in 96-well plates?
FAQ ID -1546
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Do you have a protocol for purification of total RNA from fatty tissues using QIAzol Lysis Reagent and MaXtract High Density?
FAQ ID -1550
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Which real-time PCR kits are recommended downstream of the QuantiTect Whole Transcriptome Kit?
FAQ ID -1592
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What primers are used in the reverse-transcription step of the QuantiTect Whole Transcriptome procedure?
FAQ ID -1584
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Can the AllPrep DNA/RNA/Protein Mini Kit be used with fibrous or lipid tissues?
FAQ ID -1579
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What is the maximum amount of RNA that can be used for amplification with the QuantiTect Whole Transcriptome Kit?
FAQ ID -1591
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What starting amount of RNA purified using an miRNeasy Kit should be used with the miScript System?
FAQ ID -1597
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Can RNA purified from formalin-fixed, paraffin-embedded (FFPE) tissue be used with the QuantiTect Whole Transcriptome Kit?
FAQ ID -1583
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Can samples in Allprotect Tissue Reagent undergo repeated freeze-thaws without degradation of DNA, RNA, and protein?
FAQ ID -1575
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Can the QuantiFast Probe PCR +ROX Vial Kit be used on the ABI PRISM 7900 and Applied Biosystems 7500 cyclers?
FAQ ID -1555
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Does Buffer APP in the AllPrep DNA/RNA/Protein Mini Kit contain acetone?
FAQ ID -1576
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Does cDNA amplified with the QuantiTect Whole Transcriptome Kit correspond to full-length RNA transcripts?
FAQ ID -1585
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Can tissue incubated in Allprotect Tissue Reagent be used for morphological studies?
FAQ ID -1571
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Can less than 10 ng RNA be used with the QuantiTect Whole Transcriptome Kit?
FAQ ID -1590
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Can cDNA prepared by any method be used as starting material in the ligation reaction of the QuantiTect Whole Transcriptome Kit?
FAQ ID -1589
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Can Allprotect Tissue Reagent be used to stabilize RNA, DNA and proteins in cells, bacteria, yeast, and plants?
FAQ ID -1574
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Can degraded RNA be used with the QuantiTect Whole Transcriptome Kit?
FAQ ID -1586
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How much cDNA is sufficient for real-time PCR detection of miRNA transcripts generated with the miScript System?
FAQ ID -1598
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How many samples can be run in parallel with the Rotor-Gene Q real-time PCR instrument?
FAQ ID -1519
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What main input voltage is required for the QIAxcel Analyzer?
FAQ ID -1821
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Is there a quality difference between CompactPrep Plasmid Mega and Giga and the CompactPrep Plasmid Midi and Maxi Kits?
FAQ ID -1882
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The Gel Cartridge is installed and all status indicators for the QIAxcel System are good but when clicking the "RUN" button, nothing happens. The green highlight goes down the Sequence?
FAQ ID -1850
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Why does the copy function not apply relative to migration time and normalized area values after loading the DNA size marker table and adding the alignment marker peaks on the QIAxcel System?
FAQ ID -1832
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Can customers use their own DNA size marker with the QIAxcel System and if yes, what concentration should be used?
FAQ ID -1841
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Can data be saved at once with the QIAxcel System after analysis of all samples in a 96-well PCR plate?
FAQ ID -1861
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How can a positive threshold be set when running the QIAxcel System?
FAQ ID -1843
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How long will the intensity calibration procedure on the QIAxcel System take?
FAQ ID -1826
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Will the QIAxcel System run using a green Data Review Software Key?
FAQ ID -1853
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When should an intensity calibration be re-performed on the QIAxcel System?
FAQ ID -1825
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Can the QIAxcel System or QIAxcel Advanced be connected to a network?
FAQ ID -1862
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How can a clogged capillary channel be identified when running samples on the QIAxcel System or QIAxcel Advanced?
FAQ ID -1836
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Can the QIAxcel software be loaded to another PC to view the data generated with the QIAxcel System?
FAQ ID -1855
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Can multiple QIAxcel Systems be run from one computer?
FAQ ID -1860
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How often should the solutions in the Buffer Tray for the QIAxcel System or QIAxcel Advanced be replaced?
FAQ ID -1840
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How often should the alignment marker for the QIAxcel System be changed?
FAQ ID -1839
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If the M500 method is used with the QIAxcel System, does a new DNA reference marker table have to be created?
FAQ ID -1856
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In the results table generated with the QIAxcel System, what does "normalized area" mean?
FAQ ID -1854
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What is the composition of buffer OCD?
FAQ ID -423
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What is the composition of buffer QN?
FAQ ID -414
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What is the composition of buffer OCL?
FAQ ID -422
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What is the composition of buffer OL2?
FAQ ID -424
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Can the QIAamp MinElute Virus Kits be used to extract viral nucleic acid from cell culture supernatant?
FAQ ID -472
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Can RNAi experiments be performed in Drosophila?
FAQ ID -436
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How is the RNeasy Lipid Tissue Mini Kit different from the RNeasy Mini Kit?
FAQ ID -468
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Has RNAi been successful using siRNA in Zebrafish and Xenopus?
FAQ ID -400
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Are Northern Blots sensitive enough to detect siRNA-induced gene silencing?
FAQ ID -403
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Can I use the RNeasy MinElute Cleanup Kit to clean up my in vitro transcription reaction?
FAQ ID -429
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What is the composition of buffer OEB?
FAQ ID -420
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Why is the pQE DNA provided in QIAexpress Kits blue in color?
FAQ ID -487
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What is the minimum elution volume when using the RNeasy Micro Kit?
FAQ ID -428
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What is the maximum amount of starting material that can be processed with the RNeasy Micro Kit?
FAQ ID -426
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What is the minimum elution volume when using the RNeasy MinElute Cleanup Kit?
FAQ ID -432
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What is the smallest sample size that can be used with RNeasy Mini Kits?
FAQ ID -485
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What is a homogeneous LiquiChip assay?
FAQ ID -476
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What detection reagents (reporter fluorophores) do you recommend for the LiquiChip System?
FAQ ID -480
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What are the excitation and emission wavelengths of the reporter and classification lasers of the LiquiChip Instrument?
FAQ ID -479
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What is the binding capacity of the RNeasy MinElute Cleanup Kit column?
FAQ ID -430
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What is the composition and size of the LiquiChip Beads?
FAQ ID -475
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What is the binding capacity of the RNeasy Micro Kit column?
FAQ ID -427
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Is dihydrofolate reductase (DHFR) immunogenic?
FAQ ID -471
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What are some of the assays that are adaptable to the LiquiChip System?
FAQ ID -477
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My QuantiTect Master Mix did not freeze at degrees. Is it still ok to use?
-20
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I want to start with gene silencing (RNAi) experiments. Do you have informational literature?
FAQ ID -580
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Is it possible to use less than 1 mg of protein with the PhosphoProtein Purification Kit?
FAQ ID -599
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Is it possible to use a QIAzol lysate for the isolation of RNA from tissue on the BioRobot EZ1?
FAQ ID -573
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What is a quenched FRET assay?
FAQ ID -542
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What do you recommend for isolating RNA from more than 100 mg of plant tissue?
FAQ ID -570
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What happens if I spin my lysate on the RNeasy Spin Columns at maximum speed?
FAQ ID -514
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How should I dissolve the lyophilized Anti-His and Tag Antibodies?
-100
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Is it possible to perform a UNG treatment when using QuantiTect kits?
FAQ ID -564
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Is it possible to clean up a methylation reaction containing bisulfite with QIAquick Cleanup Kits?
FAQ ID -519
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Is it possible to isolate both RNA and recombinant 6xHis-tagged protein from the same sample?
FAQ ID -532
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Why are my realtime PCR amplification plots hook-shaped?
FAQ ID -587
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Why are samples centrifuged at 4°C after the addition of chloroform when using RNeasy Lipid Tissue Kits?
FAQ ID -533
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How was it determined that the PhosphoSerine Antibody will bind phosphoserines only?
FAQ ID -579
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If pQE40 is used to express a fusion protein with DHFR, can the DHFR be cleaved afterwards?
FAQ ID -513
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Which chloroform should I use for the RNeasy Lipid Tissue Kit?
FAQ ID -516
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Will pancreas tissue swim on top of RNAprotect Tissue Reagent?
FAQ ID -536
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Will the 2x QIAGEN Multiplex PCR Master Mix freeze at -20°C?
FAQ ID - 525
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Why does my realtime PCR assay quality decrease over time?
FAQ ID -589
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What is the longest fragment that was cloned with the pQE UA Cloning Vector?
-30
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What is the difference between BSA-free and BSA-containing RGS-His Antibody?
FAQ ID -569
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When using QIAzol Lysis Reagent for RNA extraction, I see a pinkish colored aqueous phase! Is it OK?
FAQ ID -534
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Where can I find the public Smith-Waterman homology search tool that you refer to on your siRNA online design page?
FAQ ID -562
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What reaction volume should I use with QuantiTect Kits?
FAQ ID -551
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What makes QIAGEN's 10x Taq and HotStarTaq DNA Polymerase PCR buffer superior?
FAQ ID -566
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How many template copies can I detect using QuantiTect Kits in real-time PCR?
FAQ ID -543
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Can I use RNAiFect for co-transfection of plasmid DNA and siRNA?
FAQ ID -515
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Does DEPC harm RNA?
FAQ ID -529
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Does endogenously expressed DAPase in human cells degrade proteins that are expressed in cell culture?
FAQ ID -527
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Can the MAPK1 control siRNA be used for rat cells?
FAQ ID -547
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Are the columns of the MinElute Reaction Cleanup-, Gel Extraction-, and PCR Purification Kit identical?
FAQ ID -581
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After using your U/A PCR Cloning Kit I can detect colonies, which are light blue, and not really white. Why is that?
FAQ ID -512
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Can I make a master mix with QuantiProbes, primers, and all other reaction components and store it for later use?
FAQ ID -584
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Can I homogenize samples in QIAzol using the QIAshredder?
FAQ ID -501
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Can I shorten the activation time for the HotStarTaq DNA Polymerase?
FAQ ID -565
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Does QIAGEN sell 6xHis-tagged proteins besides the 6xHis Protein Ladder?
FAQ ID -594
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Do you offer QIAshredder spin columns for the RNeasy Midi/Maxi Kit format? If not, what is the alternative?
FAQ ID -560
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Do you provide control proteins for use with the PhosphoProtein Purification Kit?
FAQ ID -596
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How long should the amplicon be if I am using QuantiTect SYBR Green detection?
FAQ ID -553
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How is an antibody epitope determined?
FAQ ID -593
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Do I need to calibrate my real-time cycler if I want to use Yakima Yellow?
FAQ ID -540
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Do we recommend pooling siRNA sequences?
FAQ ID -517
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Does the His-tag interfere with the purification procedure of the QIAGEN PhosphoProtein Purification Kit?
FAQ ID -598
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Does the use of MAPK1 as a control in siRNA transfection experiments have secondary effects on the cell culture?
FAQ ID -548
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Do you have a protocol to isolate RNA from blood and bone marrow using the BioRobot EZ1?
FAQ ID -567
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Do you have any recommendations for using the PosphoProtein Kit with starting material other than cells?
FAQ ID -597
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Do you have a protocol for isolating RNA from yeast using RNeasy?
FAQ ID -535
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What is the run time for the BioSprint 96 DNA Blood protocol on the BioSprint 96 instrument?
FAQ ID -622
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Will REPLI-g work at high temperatures?
FAQ ID -656
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The Multiflow Dispense Head on my BioRobot is not dispensing the correct amount of liquid. What can I do?
FAQ ID -652
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What is the smallest amount of DNA I can put into a 50 ul REPLI-g reaction?
FAQ ID -663
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What is the recommended incubation time to stabilize tissue RNA in RNAprotect Tissue Reagent?
FAQ ID -647
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What do you mean by “Quantification of locus representation” in REPLI-g WGA product?
FAQ ID -698
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What is REPLI-g whole genome amplification?
FAQ ID -654
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What is the average amount of DNA and RNA present in 1 ml normal serum?
FAQ ID -635
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What is the biggest protein that can be synthesized with the EasyXpress Protein Synthesis System?
FAQ ID -601
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What is the length of the MDA whole genome amplification product?
FAQ ID -690
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What is the expected yield of genomic DNA isolated from bacteria with the DNeasy Blood & Tissue or QIAamp DNA Mini Kit?
FAQ ID -632
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Is it possible to perform laser microdissection with tissues stabilized with RNAprotect Tissue reagent?
FAQ ID -608
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Is it possible to synthesize membrane proteins using the EasyXpress Protein Synthesis Kit?
FAQ ID -602
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What are Elution Microtube adapters and Microplate adapters used in the DirectPrep 96 Miniprep Kit, and how can I order them?
FAQ ID -646
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What are the advantages of REPLI-g over conventional DNA sample processing and amplification methods?
FAQ ID -686
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Why do I get amplification in a negative control DNA tube using the REPLI-g Kit for WGA?
FAQ ID -675
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Why did you choose MAPK1 as a positive control in the QIAGEN RNAi Human/Mouse Control Kit?
FAQ ID -604
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Will the PhosphoSerine antibody recognize Serine residues if the neighboring residues are Leucine or Isoleucine?
FAQ ID -644
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Will TA cloning work with REPLI-g WGA product?
FAQ ID -672
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Will DNA from chromosomes collected in TE with a drop of mineral oil work with REPLI-g amplification?
FAQ ID -664
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What is the stability of the REPLI-g MDA product?
FAQ ID -693
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When delivering liquid to a 96-well plate using the MultiFlow Dispenser Head on my BioRobot , one or more rows in the plate contains less liquid per well than specified by the protocol. What is going wrong?
FAQ ID -653
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Where should I add a dye-label modification on the siRNA?
FAQ ID -648
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What types of materials from environmental samples could interfere with WGA?
FAQ ID -666
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Can I stabilize RNA in bacteria grown on solid media or substrate using the RNAprotect Bacteria Reagent?
FAQ ID -615
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Can I re-amplify whole genome amplification product?
FAQ ID -670
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Can I use another lysis buffer than the one supplied in the PhophoProtein Purification Kit?
FAQ ID -645
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Can I run less than 15 samples on the BioSprint 15 instrument?
FAQ ID -621
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Can the QIAshredder Maxi Spin Columns from the DNeasy Plant Maxi Kit be used for RNeasy samples?
FAQ ID -616
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Do you have an off-the shelf diagnostics kit for WGA?
FAQ ID -678
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Are there components in biological samples that can inhibit the REPLI-g reaction?
FAQ ID -667
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Are the BioSprint 15/96 instruments stand-alone workstations?
FAQ ID -620
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Can the computer for the LiquiChip Workstation be networked?
FAQ ID -629
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Are QIAGEN EZ Competent Cells of the QIAGEN PCR Cloning Kit dam+ or dam-?
FAQ ID -638
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Are samples that are contaminated with high molecular weight DNA (e.g. from a paramedic or police officer) a problem with REPLI-g amplification?
FAQ ID -683
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Can BAC DNA prepared with the R.E.A.L. Prep 96 Plasmid Kit be used for high-throughput sequencing?
FAQ ID -643
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Can I do whole genome amplification from brain tissue using REPLI-g?
FAQ ID -669
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Can I do whole genome amplification from mitochondrial DNA using REPLI-g?
FAQ ID -668
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Can FTA paper for dried blood spots be used with REPLI-g?
FAQ ID -685
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How can I quantify the amount of REPLI-g DNA I have amplified?
FAQ ID -694
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Can I amplify from plasma or serum samples using the REPLI-g Mini/Midi kits?
FAQ ID -671
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How should I store the REPLI-g amplification product?
FAQ ID -692
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Is 2 kb the minimal gDNA fragment size for REPLI-g Whole Genome Amplification?
FAQ ID -682
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How long can I store bacterial culture stabilized in RNAprotect Bacteria Reagent?
FAQ ID -614
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Do I need to clean up the REPLI-g amplification product for downstream analysis?
FAQ ID -691
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Do you have a kit for RNA isolation from any kind of sample type?
FAQ ID -627
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Does REPLI-g technology for whole genome amplification work with paraffin embedded samples?
FAQ ID -673
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How can I determine the quality of my REPLI-g amplified products?
FAQ ID -695
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How can I decrease ribosomal RNA (rRNA) contamination using Oligotex?
FAQ ID -642
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Do you know of anything in the buffers/DNA sample that can cause bias in REPLI-g WGA?
FAQ ID -684
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Do you have a protocol for the purification of DNA from rodent tail using the BioSprint System?
FAQ ID -979
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Do you have a protocol for the isolation of DNA from epithelial cells mixed with sperm cells?
FAQ ID -926
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Do you have a protocol for isolation of genomic DNA from saliva using the DNeasy Blood & Tissue Kit?
FAQ ID -931
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Do you have a protocol for purification of DNA from clotted blood using the FlexiGene DNA Kit?
FAQ ID -973
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Do you have a protocol for Streptokinase treatment of clotted blood?
FAQ ID -912
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Do you have a protocol for the isolation of total nucleic acids from animal and human cells on the BioRobot EZ1?
FAQ ID -974
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Do you have a protocol for the isolation of total RNA from plant tissue using QIAGEN-tips?
FAQ ID -949
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Do you have a protocol for the isolation of total RNA from buccal swabs?
FAQ ID -956
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Do you have a protocol for the isolation of viral DNA from stool?
FAQ ID -929
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Do you have a protocol for the isolation of total RNA from plants using the RNeasy 96 Kit?
FAQ ID -951
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Do you have a protocol for the isolation of DNA from formalin-preserved stool samples?
FAQ ID -922
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Do you have a protocol for the isolation of genomic DNA from fungi?
FAQ ID -911
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Do you have a protocol for the isolation of genomic DNA and/or proteins from fatty tissue treated with QIAzol?
FAQ ID -955
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Do you have a protocol for the purification of DNA from cells using the BioSprint System?
FAQ ID -977
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Do you have a protocol for the purification of DNA from dried blood spots using the BioSprint System?
FAQ ID -982
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Do you have a protocol for the preparation of tissues preserved in RNAprotect Tissue Reagent for pathological studies?
FAQ ID -950
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Do you have a protocol for the purification of DNA from buccal swabs using the BioSprint System?
FAQ ID -983
View
Do you have a protocol for isolation of genomic and viral DNA from lymphocytes using QIAGEN Genomic-tips?
FAQ ID -901
View
Do you have a protocol for isolation of genomic and plasmid DNA from cell cultures using QIAGEN Genomic-tips?
FAQ ID -902
View
Do you have a protocol for transfection of adherent cells with low concentrations of siRNA using RNAiFect Transfection Reagent?
FAQ ID -970
View
Do you have a protocol for GMO testing of food samples?
FAQ ID -921
View
Do you have a protocol for isolation of bacterial DNA from soil?
FAQ ID -923
View
Do you have a protocol for isolation of genomic DNA from dried blood spots using the QIAamp 96 DNA Blood Kit?
FAQ ID -919
View
Do you have a protocol for isolation of genomic DNA from insects?
FAQ ID -904
View
Do you have a protocol for detection of HBV DNA by PCR?
FAQ ID -915
View
Does QIAGEN sell a vector with a T7 promotor for use with the EasyXpress Protein Synthesis Insect Kit?
FAQ ID -937
View
Can the BioSprint instruments be placed in a cool room?
FAQ ID -938
View
Do you have a protocol for Acetyl Cysteine (NALC) treatment of viscous samples?
FAQ ID -913
View
Do you have a protocol for co-transfection of adherent cells with siRNA and plasmid DNA?
FAQ ID -969
View
Do you have a protocol for adding new reporter dyes to the ABI PRISM 7900 Sequence Detection System?
FAQ ID -965
View
Do you have a protocol for cleanup of non-radioactive DNA samples?
FAQ ID -945
View
Do you have a protocol for radioactive labelling of cDNA?
FAQ ID -960
View
Do you have protocols for multiple extractions of DNA fragments from agarose gels?
FAQ ID -944
View
Why is it not recommended to stabilize cells with RNAprotect Tissue Reagent?
FAQ ID -941
View
Do you have a protocol for the detection of Bordetella pertussis DNA by PCR?
FAQ ID -914
View
Do you have a protocol for the isolation of DNA from cultured cells using the BioRobot EZ1?
FAQ ID -990
View
Do you have a protocol for performing quantitative multiplex PCR on the LightCycler 2.0 system?
FAQ ID -966
View
Do you have a protocol for long-term, siRNA-mediated gene silencing using HiPerFect Transfection Reagent?
FAQ ID -972
View
Do you have a protocol for manual purification of 6xHis-tagged proteins expressed in E. coli using Ni-NTA Superflow?
FAQ ID -967
View
Do you have a protocol for purification of DNA from cultured animal cells using the DNeasy 96 Blood & Tissue Kit?
FAQ ID -934
View
Do you have a protocol for purifying 2x96 PCR samples simultaneously with the QIAquick 96 PCR Purification Kit?
FAQ ID -935
View
Do you have a protocol for purification of DNA fragments from Cy3/Cy5 dye-label reactions?
FAQ ID -947
View
Do you have a protocol for polyacrylamide gel analysis of oligonucleotides?
FAQ ID -961
View
Do you have a protocol for isolation of genomic DNA from plants and filamentous fungi using QIAGEN Genomic-tips?
FAQ ID -905
View
Do you have a protocol for isolation of genomic DNA from flies using QIAGEN Genomic-tips?
FAQ ID -903
View
What are exo-resistant random hexamers used in the REPLI-g reaction?
FAQ ID -710
View
The Sigma Centrifuge makes a loud noise at low speeds which disappears at higher speeds, when using the 2x96-well plate rotor. What is the problem?
FAQ ID -776
View
Is RNAprotect Bacteria Reagent compatible with the RNeasy Mini Kit?
FAQ ID -797
View
What is the basic technology behind the Strep-tag Protein Purification System?
FAQ ID -740
View
What are the expected average DNA yields from plant extractions on the BioSprint 96?
FAQ ID -771
View
If template abundance differs significantly between housekeeping and target gene, are both amplified equally efficient with the QuantiTect Multiplex Kits?
FAQ ID -718
View
How to quantitate DNA purified using the BioSprint 96?
FAQ ID -773
View
How to store DNA from forensic samples eluted into water on the EZ1 biorobot in TE buffer instead?
FAQ ID -734
View
Is it possible to use LiquiChip beads for FACS applications?
FAQ ID -733
View
Is it possible to buy the EZ Competent Cells of the QIAGEN PCR Cloning Plus Kit separately?
FAQ ID -763
View
Is GFP excited and detected by the green laser of the LiquiChip System?
FAQ ID -737
View
Which Qproteome or Protein Fractionation Kits from QIAGEN are compatible with tissue samples?
FAQ ID -755
View
Why are some of my RNAprotect-stabilized samples frozen at 0ºC while others are not?
FAQ ID -758
View
Why are the QuantiTect and QuantiFast Multiplex PCR Kits limited to triplex real-time PCR on some cyclers?
FAQ ID -715
View
Why should DNA or cDNA targets be less than 250 bp long for real-time PCR?
FAQ ID-751
View
Where can I find info on compatible reporter dyes for use in real-time multiplex PCR using the QuantiTect Multiplex PCR Kits?
FAQ ID -719
View
Why might my gene of interest drop out during WGA if it is not near a telomere or centromere?
FAQ ID -703
View
Will the random hexamers in the REPLI-g reaction interfere with downstream analysis?
FAQ ID -713
View
Why is there no detection of free reporter fluorescence in the LiquiChip System?
FAQ ID -732
View
What is the cell number range one can use with the FastLane Cell cDNA Kit for real-time RT-PCR?
FAQ ID -782
View
What negative (unphosphorylated) and positive (phosphorylated) control proteins do you recommend for use with the PhosphoProtein Purification Kit?
FAQ ID -779
View
What is the enzyme used in the REPLI-g reaction?
FAQ ID -704
View
What type of Sequencing Services does QIAGEN offer?
FAQ ID -781
View
Where can I find a copy of the report files generated by running accessory protocols "Dilutor Delivery (monthly)" and "High-Speed Pipetting Sytem (monthly)" on my BioRobot 9604?
FAQ ID -766
View
What is the general sensitivity level of the LiquiChip System?
FAQ ID -731
View
Can QIAGEN's Taq- and HotstarTaq DNA Polymerases be used for cycle sequencing?
FAQ ID -741
View
Can I use the PhosphoProtein Purification Kit to purify phosphorylated peptides of around 1 kD size?
FAQ ID -778
View
Can RNAprotect Tissue Reagent be used to stabilize tissue which has already been flash-frozen?
FAQ ID -757
View
Can lyophilized plant material be used for DNA extraction on the BioSprint 96?
FAQ ID -772
View
Can the REPLI-g WGA enzyme work with labeled dNTPs?
FAQ ID -706
View
Can the MAPK1 siRNA of the RNAi Human/Mouse Starter Kit be purchased separately?
FAQ ID -744
View
Can the QIAvac 96 vacuum manifold be used with DirectPrep 96 plates?
FAQ ID -722
View
Are the buffers in the Ni-NTA Fast Start Kit the same as the ones for use with Ni-NTA purchased separately?
FAQ ID -791
View
Any data on the fidelity of the REPLI-g MDA technique?
FAQ ID -707
View
Are Centromeres and Telomeres amplified using REPLI-g WGA?
FAQ ID -702
View
Can I use my own primers for REPLI-g WGA to amplify a specific chromosomal region?
FAQ ID -712
View
Can I do 4-plex real-time PCR, on the ABI PRISM 7000, 7700, or 7900 using the QuantiTect Multiplex PCR Kit?
FAQ ID -716
View
How can one perform multiplex real-time PCR analysis using the LightCycler 2.0?
FAQ ID -780
View
How can I be sure that I am harvesting my induced bacterial culture at the best time point for protein expression?
FAQ ID -788
View
How do the FastLane Cell cDNA Kit and QuantiTect Reverse Transcription Kit eliminate genomic DNA contamination?
FAQ ID -783
View
How many cells can be used with the FastLane Cell cDNA Kit?
FAQ ID -796
View
Do you have an antibody for Western Blot Analysis of MAPK1 gene knockdown using your MAPK1 control siRNA?
FAQ ID -746
View
Can frozen blood be used for whole genome amplification with REPLI-g?
FAQ ID -794
View
Do I have to switch off the laser of the LiquiChip Workstation overnight?
FAQ ID -736
View
How many reporter dyes can be used in the same analysis using the LiquiChip Workstation?
FAQ ID -738
View
Do I need to determine limiting primer concentrations with the QuantiTect Multiplex PCR Kits?
FAQ ID -714
View
Do you synthesize thiophosphate modified random hexamers?
FAQ ID -711
View
Has anyone verified whole genome amplification accuracy with Sequencing?
FAQ ID -701
View
Do you have a protocol for the fixation of cells transfected with fluorescently labeled siRNA?
FAQ ID -793
View
How best to handle the light sensitive LiquiChip reagents?
FAQ ID -735
View
Do you have information on the use of recombinant DNA and RNA as absolute standards for realtime RT-PCR?
FAQ ID -729
View
Is it possible to transfect DNA into insect cell lines such as Drosophila melanogaster S2?
FAQ ID -397
View
Is it possible to isolate free genomic DNA using the QIAamp UltraSens Virus Kit?
FAQ ID -358
View
Is it possible to store TAGzyme enzymes at°C?
-80
View
I accidentally added RNAprotect Tissue Reagent to my cells, and now the cells are difficult to pellet. What can I do?
FAQ ID -364
View
What are the calculated molecular weights of the TAGZyme enzymes?
FAQ ID -329
View
How complete is the removal of DAPase in the TAGZyme process?
FAQ ID -319
View
How long is fluorescence detectable in cells after transfection with fluorescently labeled siRNAs?
FAQ ID -392
View
How do I submit a siRNA order by telephone or online?
FAQ ID -399
View
How many transfections can I perform with 20 nmol of HPP grade siRNA?
FAQ ID -367
View
Which primers can I use for sequencing pQE-expression vector constructs?
FAQ ID -343
View
What is the standard assay volume recommended for LiquiChip assays?
FAQ ID -330
View
What is the acceptable maximum g force for centrifugation of Xa Removal Resin?
FAQ ID -320
View
What is the lowest elution volume that can be used with QIAprecipitator Midi and Maxi Modules?
FAQ ID -307
View
What is the composition of Buffer FG3?
FAQ ID -356
View
Can I use ethanol instead of isopropanol for DNA precipitation when using HiSpeed Plasmid Kits?
FAQ ID -354
View
303 - Can I use my own lysis buffer with the DNeasy Blood & Tissue or QIAamp DNA Mini Kit?
View
Can I use a partial MinElute 96 UF plate?
FAQ ID -355
View
Can I use the RNeasy Mini Kit or RNeasy 96 Kit with fewer than 100 cells?
FAQ ID -372
View
Can I use water for elution from the QIAprecipitator of the HiSpeed Plasmid Kits?
FAQ ID -306
View
Are the QIAprecipitator Midi and Maxi Modules of the HiSpeed Plasmid Kits interchangeable?
FAQ ID -308
View
Apart from the antibody itself, does the Penta-His Biotin Conjugate solution contain any protein?
FAQ ID -359
View
Are QIAGEN monoclonal Anti His Antibodies glycosylated?
FAQ ID -334
View
Can I perform in-vitro transcription with the pDrive Cloning Vector from the QIAGEN PCR Cloning Kit?
FAQ ID -332
View
Do the QuantiTect SYBR Green Kits contain a stabilization reagent that inhibits the sensitivity of real-time PCR?
FAQ ID -328
View
Do I need to anneal, deprotect or desalt my QIAGEN siRNA?
FAQ ID -398
View
How are siRNAs introduced into C. elegans?
FAQ ID -396
View
Do pQE vectors contain the CAT gene?
FAQ ID -362
View
How can I improve the expression of proteins containing hydrophobic regions?
FAQ ID -339
View
How can I check if any residual proteins remain on the Ni-NTA Agarose matrix after elution?
FAQ ID -324
View
Do freeze-thaw cycles affect the stability of the U-overhang on the pDrive Cloning Vector?
FAQ ID -323
View
Can RNAprotect Tissue Reagent be used to store previously isolated RNA?
FAQ ID -337
View
Can MinElute 96 UF PCR be used to purify PCR samples which contain mineral oil?
FAQ ID -360
View
Does SYBR Green dye present in QuantiTect kits interfere with ethidium bromide staining?
FAQ ID -312
View
Do any of the buffers in the HotStarTaq DNA Polymerase Kit contain Triton?
FAQ ID -327
View
Do any of the kit components or the product packaging of the QIAamp 96 DNA Blood Kit contain latex?
FAQ ID -318
View
What data documentation does QIAGEN's Sequencing Service provide for customers?
FAQ ID -832
View
What are the sequences of the FlexiTube siRNAs?
FAQ ID -851
View
My BioRobot 9604 came with several Accessory Applications. Is it recommended I run these protocols?
FAQ ID -872
View
What is the proper way to turn on or turn off the EZ1 instrument?
FAQ ID -870
View
What is the Energy Mix in the EasyXpress Kits?
FAQ ID -846
View
What is the concentration of the primers in a reaction using the QuantiTect Primer Assays?
FAQ ID -850
View
I would like to sequence my siRNA constructs. Does QIAGEN offer Sequencing Services for cloned siRNA?
FAQ ID -829
View
Is it possible to use frozen cell pellets with the FastLane Cell cDNA Kit?
FAQ ID -835
View
Is it possible to elute plasmid DNA from the QIAprep Spin Miniprep columns with buffer containing Potassium Phosphate?
FAQ ID -854
View
Is it possible to perform immunoprecipitation (IP) directly on protein fractions from the Qproteome Cell Compartment Kit?
FAQ ID -855
View
Is it possible to adjust the Lysis Buffer volume used with the Qproteome Bacterial Protein Preparation Kit?
FAQ ID -841
View
Is it possible to use QIAGEN's pQE-TriSystem Vectors with the EasyXpress Protein Synthesis Insect Kit?
FAQ ID -876
View
Is there a stopping point in the protocol for Suspended Cells using the FastLane Cell cDNA Kit?
FAQ ID -837
View
Is the random biotin labeling of proteins expressed with your EasyXpress Biotin Labeling Kit as sensitive as labeling with radioactivity?
FAQ ID -875
View
Will biotin detection assays be efficient when doing in vitro translation from E. coli lysates with the EasyXpress Site-Specific Biotin Kit?
FAQ ID -878
View
Why would clumps occur following the addition of Buffer P2 when using LyseBlue Reagent in a plasmid preparation?
FAQ ID -862
View
Will acetone precipitation recommended in the Qproteome Protocols denature Protein?
FAQ ID -807
View
Will site-specific biotin labeling with the EasyXpress Site-Specific Biotin kit alter the structure and function of my protein?
FAQ ID -877
View
Will the sequences of the QuantiTect Primer Assays be provided?
FAQ ID -804
View
Will the FastLane Cell cDNA Kit work with suspension cells?
FAQ ID -801
View
Which resin is used in the QIAexpress Ni-NTA Fast Start Columns?
FAQ ID -836
View
What publicly funded Genome Sequencing Projects has QIAGEN's Sequencing Service been involved in?
FAQ ID -815
View
What is the typical yield of site-specific labeled protein generated using the EasyXpress Site-Specific Biotin Kit?
FAQ ID -873
View
What programs does your Sequencing Service use to ensure accurate base calling, Sequence Quality, and Alignment?
FAQ ID -831
View
Where can I find a copy of the report file generated by running 'Maintenance Protocol I' on my BioRobot MDx?
FAQ ID -806
View
Which fraction will contain soluble mitochondrial proteins using the Qproteome Cell Compartment Kit?
FAQ ID -839
View
Which fraction will contain endosomal, microsomal and lysosomal proteins using the Qproteome Cell Compartment Kit?
FAQ ID -808
View
Where can I find QIAGEN products for a specific gene or gene product?
FAQ ID -803
View
Can the QuantiTect Primer Assays be used for detection of genomic DNA?
FAQ ID -811
View
Do I need to use RNase inhibitors with the RNeasy Kits?
FAQ ID -813
View
Do the Buffers CE1 - CE4 of the Qproteome Cell Compartment Kit contain DTT or any reducing agent?
FAQ ID -824
View
Can the FastLane Cell cDNA Kit also be used for qualitative end-point PCR?
FAQ ID -834
View
Can the FastLane Cell cDNA Kit be used with tissue?
FAQ ID -821
View
Do you have a protocol for the isolation of bacteriophage P1 derived constructs with any of your plasmid kits?
FAQ ID -883
View
Do you have a protocol for the isolation of BAC DNA using the QIAGEN Plasmid Midi Kit?
FAQ ID -881
View
895 - Do you have a protocol for the isolation of endotoxin-free plasmid DNA using the QIAGEN Plasmid Midi Kit?
View
Do you have a protocol for isolation of plasmid DNA using the Sigma Centrifuge 4?
-15
View
Can Buffer R1 of the R.E.A.L. Prep 96 Plasmid Kit be replaced with Buffer P1?
FAQ ID -823
View
Are there specific recommendations for performing RT-PCR on RNA isolated from paraffin-embedded samples?
FAQ ID -828
View
Can I stabilize adipose tissue using RNAprotect Tissue Reagent prior to RNA isolation?
FAQ ID -856
View
Can I use the PhosphoProtein Purification Kit for the isolation of phosphorylated proteins from yeast?
FAQ ID -838
View
Can I use QIAGEN's pQE vectors as templates in the EasyXpress Biotin Labeling Kits?
FAQ ID -874
View
Can I use my gene-specific primers with the FastLane Cell cDNA Kit and the QuantiTect Reverse Transcription Kit?
FAQ ID -812
View
Can I use LyseBlue with R.E.A.L. Prep 96, QIAwell Ultra, or QIAprep 96 Turbo Miniprep Kits for the BioRobot systems?
FAQ ID -863
View
Do you have information on in vivo RNA interference experiments?
FAQ ID -817
View
Do you sell the ATP-Dependent Exonuclease of the QIAGEN Large-Construct Kit separately?
FAQ ID -825
View
I cannot see the LCD display fonts on the Sigma Centrifuge. How do I adjust the contrast?
FAQ ID -822
View
How long does a typical in vitro translation reaction take with your large-scale EasyXpress protein synthesis kits?
FAQ ID -880
View
How much protein can be produced with the EasyXpress Protein Synthesis Mega Kit?
FAQ ID -843
View
Do you have a protocol for the isolation of very low-copy plasmids from Streptomyces spp.?
FAQ ID -893
View
Do you have a protocol for the isolation of plasmid DNA from Citrobacter freundii?
FAQ ID -885
View
Do you have a protocol for the isolation of plasmid DNA from Corynebacterium glutamicum?
FAQ ID -886
View
Do you have a protocol for the isolation of plasmid DNA from Borrelia spp.?
FAQ ID -884
View
Do you have a protocol for the isolation of plasmid DNA from Lactobacillus spp.?
FAQ ID -887
View
Do you have a protocol for the isolation of plasmid DNA from yeast?
FAQ ID -853
View
Do you have a protocol for the isolation of single-stranded DNA from M13 phage using QIAGEN Plasmid Kits?
FAQ ID -894
View
Do you have a protocol for the isolation of plasmid DNA from Staphylococcus spp.?
FAQ ID -890
View
Do you have a protocol for the isolation of plasmid DNA from Oligotropha carboxidovorans?
FAQ ID -888
View
Do you have a protocol for the isolation of plasmid DNA from Proteus spp.?
FAQ ID -889
View
What are your recommendations for cotransfecting several plasmids?
FAQ ID -124
View
What are the recommended culture and buffer volumes for a very low-copy plasmid?
FAQ ID -168
View
Is mRNA isolation necessary for sensitive RT-PCR?
FAQ ID -111
View
When should carrier be used with the QIAamp DNA Mini or the DNeasy Blood & Tissue Kit?
FAQ ID -100
View
Which Anti-His Antibody is the most sensitive for my protein of interest?
FAQ ID -172
View
Why do I have to add beta-mercaptoethanol (beta-ME) to lysis Buffer RLT of the RNeasy Kits?
FAQ ID -101
View
What is the recommended culture medium for the QIAprep System?
FAQ ID -154
View
How can I eliminate contaminating protein in my Ni-NTA 6xHis-tag protein purification?
FAQ ID -102
View
How can I purify very small amounts of 6xHis-tagged protein using Ni-NTA technology?
FAQ ID -134
View
How can I improve ligation efficiency of DNA from a QIAEX II Gel Extraction Kit?
FAQ ID -141
View
How can I isolate RNA from 1 gram of plant tissue?
FAQ ID -128
View
How can I improve the performance of the HiSpeed QIAprecipitator module?
FAQ ID -144
View
What can I use to isolate RNA smaller than 200 nucleotides?
FAQ ID -115
View
What epitopes do the Anti-His Conjugates and Anti-His Antibodies recognize?
FAQ ID -170
View
What are the maximum culture volumes to use with the QIAGEN Plasmid Midi or Maxi Kit?
FAQ ID -167
View
How should RNeasy Kits be stored and how long are they stable?
FAQ ID -103
View
Is it possible to cleave the 6xHis-tag from an expressed protein?
FAQ ID -140
View
How should QIAGEN Plasmid Purification Kits be stored and for how long?
FAQ ID -192
View
What are the features and benefits of the QIAexpress 6xHis Tag System?
FAQ ID -193
View
Do you have transfection data for QIAGEN Transfection Reagents?
FAQ ID -158
View
Do you have data showing effects of sample size on DNA yield and purity using the DNeasy 96 Blood & Tissue kit?
FAQ ID -129
View
Does QIAGEN offer vectors for direct cloning of PCR products?
FAQ ID -146
View
How can I avoid variable transfection efficiencies with SuperFect Reagent?
FAQ ID -179
View
Can DyeEx be used to clean up labeled DNA other than sequencing templates?
FAQ ID -137
View
Are QIAamp DNA isolation kits suitable for apoptosis studies?
FAQ ID -149
View
Can I use a QIAfilter Cartridge for purifying large plasmids?
FAQ ID -121
View
Can I use Omniscript or Sensiscript RT's at a higher temperature?
FAQ ID -116
View
How do you ensure that RNeasy buffers are RNase-free?
FAQ ID -113
View
How can I purify DNA from soil, food and sewage samples for PCR?
FAQ ID -118
View
How should I store Anti·His and Tag·100 Antibodies?
FAQ ID -194
View
How do I prepare an insert for pQE vectors?
FAQ ID -185
View
How can I obtain DNA-free RNA using an RNeasy Midi or Maxi Kit?
FAQ ID -143
View
What are the compatibilities of different reagents with Ni-NTA matrices?
FAQ ID -49
View
How can I prevent running out of M48 Kit reagents before the full number of samples are processed?
FAQ ID -1042
View
Do you have a protocol for the isolation of genomic DNA from paraffin-embedded samples on the BioRobot M48?
FAQ ID -1019
View
Is the TurboCapture Microplate Holder Type B compatible with the Biomek FX robotic workstation?
FAQ ID -1040
View
Is there a limit to the size of proteins that can be purified using the Qproteome Glycoprotein Kits?
FAQ ID -1071
View
Is it possible to scale up QuantiTect Reverse Transcription reactions to allow use of larger amounts of RNA?
FAQ ID -1063
View
Is it possible to use the PhosphoProtein Purification Kit in batch format?
FAQ ID -1069
View
What are the recommended storage conditions for the QuantiTect Reverse Transcription Kit and its components?
FAQ ID -1077
View
What is the difference between TurboCapture Microplate Holders Type A and Type B?
FAQ ID -1038
View
What blocking reagents do you recommend for chemiluminescent detection using Penta-, Tetra-, and RGS-His antibodies?
FAQ ID -1093
View
Is Factor SB of the HotStar HiFidelity Polymerase Kit the same as Factor MP of the QIAGEN Multiplex PCR Kit?
FAQ ID -1049
View
How can I transfect siRNA into insect cells such as Drosophila melanogaster S2?
FAQ ID -1046
View
How can I tell TurboCapture Microplate Holder Type A from Type B?
FAQ ID -1039
View
How much template can I use in the reaction and what is the maximum volume of template that can be used in the QuantiTect Kits?
FAQ ID -1086
View
How much dye-labeled siRNA should I use to monitor transfection efficiency when using HiPerfect Transfection Reagent?
FAQ ID -1058
View
What is the error rate of HotStar HiFidelity DNA Polymerase?
FAQ ID -1048
View
Which QIAGEN kit do you recommend for purifying plasmid DNA suitable for transfection of sensitive cells?
FAQ ID -1092
View
Which kit should I use for RNA isolation from Cartilage?
FAQ ID -1026
View
Where can I find the serial number on QIAGEN's BioRobots?
FAQ ID -1064
View
Which kit can be used for simultaneous purification of DNA and RNA from biopsies?
FAQ ID -1089
View
Why is there RNA in the DNA eluate when using the AllPrep DNA Mini Spin Column?
FAQ ID -1044
View
Why does my PCR product show up later when comparing the QuantiTect SYBR Green PCR Kits with Roche kits using the same annealing temperature?
FAQ ID -1083
View
Will the "classic" RNeasy Mini Kit be discontinued after the launch of the Allprep DNA/RNA Mini Kit?
FAQ ID -1054
View
Why does TA/UA cloning work with your proofreading HotStar HiFidelity DNA Polymerase?
FAQ ID -1053
View
Why do melting temperatures differ between PCR fragments amplified with QIAGEN's QuantiTect SYBR Green PCR Kits and Roche Kits?
FAQ ID -1084
View
What is the range of melting temperatures for the QuantiTect Primer Assay amplicons in the dissociation curve?
FAQ ID -1079
View
What is the protein loading capacity per column of the Qproteome Albumin/IgG Depletion Kit?
FAQ ID -1090
View
What is your recommendation for cleaning the TissueLyser Bead Dispenser (96-well format)?
FAQ ID -1055
View
What is the resin in the Qproteome Murine Albumin and Qproteome Albumin/IgG Depletion Kits?
FAQ ID -1034
View
How can I prevent clogging of QIAfilter cartridges?
FAQ ID -1060
View
What is the volume of the eluate when using either spin or vacuum procedures with the QIAprep 96 Turbo Miniprep Kit?
FAQ ID -1067
View
Do I have to use a ramp time of 2°C/sec on the LightCycler when using Quantitect Primer Assays?
FAQ ID -1065
View
Does an activation time of 15 minutes influence the performance of the HotStarTaq Plus DNA Polymerase?
FAQ ID -1050
View
Do you have a protocol for the isolation of RNA from bacterial cultures using RNAprotect Bacteria Reagent and QIAzol Lysis Reagent?
FAQ ID -1020
View
Do you have a protocol for the isolation of DNA from mouse tails using MagAttract technology on BioRobot M96?
FAQ ID -1007
View
Do you have a protocol for the isolation of DNA from forensic samples using the BioRobot M48?
FAQ ID -1017
View
Can the USB Port of the BioSprint 96 instrument be used to transfer programs from a PC to the instrument?
FAQ ID -1032
View
Are your FlexiTube siRNAs preferentially designed to any particular region of the target gene?
FAQ ID -1091
View
Can PCR fragments generated with the EasyXpress Linear Template Kit be cloned into the pIX4.0 vector of the EasyXpress Protein Synthesis Insect Kit?
FAQ ID -1078
View
Can the QProteome O-Glycan Glycoprotein Kits be used for blood cells?
FAQ ID -1036
View
Can the Reverse Transcriptases of the QuantiTect Reverse Transcription Kit and the QuantiTect Probe RT-PCR Kit be used interchangeably?
FAQ ID -1066
View
Can the AllPrep RNA/Protein Kit be used with tissue?
FAQ ID -1095
View
Can the Blocking Reagent supplied with the Penta-His HRP Conjugate be used with other antibodies?
FAQ ID -1073
View
How can air bubbles in PhosphoProtein Purification Columns be avoided?
FAQ ID -1070
View
How can I increase low-copy plasmid DNA yields using QIAfilter Plasmid Kits?
FAQ ID -1062
View
How can I ensure complete genomic DNA removal when using the RNase-Free DNase Set?
FAQ ID -1087
View
Do you have information on the reproducibility of DNA yields from blood using the M48 BioRobot?
FAQ ID - 1004
View
Do you have a protocol for transient transfection of HEK 293 cells in 75 cm3 flasks?
FAQ ID -1014
View
Do you have a protocol for transient transfection of cells in 96-well plates using PolyFect Transfection Reagent?
FAQ ID -1013
View
Do you have a protocol for transient transfection of HeLa-S3 cells in 60 mm dishes using PolyFect?
FAQ ID -1012
View
Do you have a protocol for the isolation of total nucleic acids from cells on the M48 BioRobot?
FAQ ID -1009
View
Do you have a protocol for vacuum processing of blood samples with the QIAamp DNA Blood Midi or Maxi Kits?
FAQ ID -1011
View
Do you have a protocol for the isolation of total nucleic acids from tissue on the M48 BioRobot?
FAQ ID -1008
View
Do you have information on realtime RT-PCR performance of RNA isolated from monocytes on the M48 BioRobot?
FAQ ID -1010
View
Do you have information on DNA yields from blood with variable numbers of white blood cells using the M48 BioRobot?
FAQ ID -1006
View
Do you have information on PCR template preparation from preserved blood samples on the M48 BioRobot?
FAQ ID - 1003
View
Can the PAXgene Blood RNA Tubes undergo freeze/thaw cycles?
FAQ ID -2471
View
If I want to use BioMag particles, do I have to buy a QIAGEN magnet too or can I use one that I already have?
FAQ ID -266
View
Should I use Omniscript or Sensiscript for reverse transcription of low-copy mRNA?
FAQ ID -297
View
What are the main differences between other magnetic beads and the BioMag particles?
FAQ ID -272
View
My BioMag is about to expire, is it still any good?
FAQ ID -268
View
How magnetically responsive is BioMag?
FAQ ID -265
View
If I am using BioMag Streptavidin to capture biotinylated oligos, how can I then remove the oligos?
FAQ ID -279
View
Why is an ice-incubation step included during reaction set-up when following the QuantiTect RT-PCR but not the QuantiTect PCR protocol.
FAQ ID -283
View
What sequencing primers can I use with the pDrive cloning vector of the QIAGEN PCR Cloning Kit?
FAQ ID -292
View
What is the difference between the QIAamp MinElute Virus Spin and the MinElute Virus Vacuum Kit?
FAQ ID -295
View
What is the difference between the Fc specific versions of your secondary antibody BioMag particles and the non-specific?
FAQ ID -275
View
Can I use my own cycling conditions with the QIAGEN Multiplex PCR Kit?
FAQ ID -287
View
Can I select FITC labeled cells with BioMag?
FAQ ID -278
View
Can I use BioMag particles with any specific primary antibody?
FAQ ID -273
View
Are BioMag particles stable in other solvents?
FAQ ID -267
View
Can BioMag be used for positive and negative selection?
FAQ ID -269
View
How can I avoid poor immunolocalization morphology with Anti-His Antibodies?
FAQ ID -200
View
How can I prevent non-specific binding when using BioMag?
FAQ ID -277
View
How do I prevent bubbles from forming in my Ni-NTA agarose column?
FAQ ID -285
View
How can I purify mRNA from total RNA using an Oligotex Direct mRNA Kit?
FAQ ID -201
View
Is your Nuclease-Free Water fluorescence-free?
FAQ ID -1291
View
What quality level does your Nuclease-Free Water have?
FAQ ID -1292
View
How should the GelPilot DNA Loading Dye be handled?
FAQ ID -1287
View
Does GelPilot DNA Loading Dye contain ethidium bromide?
FAQ ID -1286
View
Can phenol/chloroform be placed in the MaXtract tube for extraction at a later time?
FAQ ID -1299
View
Are MaXtract tubes siliconized?
FAQ ID -1298
View
Should I input the dilution factor of my samples into the LiquiChip Analyser Software dilution factors of samples?
FAQ ID -1211
View
What cell lines have been tested with the Qproteome Plasma Membrane Protein Kit?
FAQ ID -1205
View
Is a saliva collection device provided in the RNeasy Protect Saliva Mini Kit?
FAQ ID -1213
View
Is it possible to increase nucleic acid concentrations when following protocols on the BioRobot EZ1?
FAQ ID -1232
View
Is RNAprotect Cell Reagent provided in the RNeasy Protect Cell Kit available separately?
FAQ ID -1217
View
Is it possible to use QIAGEN's pQE-Trisystem Vector with the EasyXpress Insect Kit II?
FAQ ID -1225
View
What vector do you recommend as template for in-vitro protein expression with the EasyXpress Insect Kit II?
FAQ ID -1224
View
What type of promoter should my template have for in-vitro protein expression with the EasyXpress Insect Kit II?
FAQ ID -1223
View
What is the maximum number of cells that can be used with the AllPrep RNA/Protein Kit ?
FAQ ID -1208
View
Will the Qproteome Plasma Membrane Protein Kit work with starting materials other than adherent mammalian cells?
FAQ ID -1202
View
What is the difference between various FlexiTube siRNAs listed for the same target gene, and which one should I choose?
FAQ ID -1206
View
What is the protein yield when using the Qproteome Plasma Membrane Protein Kit?
FAQ ID -1201
View
What is the size of the BioRobot EZ1?
FAQ ID -1245
View
Does anything have to be added to express post-translational modifications (PTMs) with the EasyXpress Insect Kit II?
FAQ ID -1226
View
Do you have a protocol for isolating cytoplasmic RNA from animal cells using the RNeasy Mini Kit?
FAQ ID -1207
View
Do you have a protocol for purification of miRNA from animal cells using the RNeasy Plus Mini Kit and RNeasy MinElute Cleanup Kit?
FAQ ID -1258
View
Can cells stabilized with RNAprotect Cell Reagent be used for cell sorting by flow cytometry (FACS)?
FAQ ID -1216
View
Can glycosylated proteins be expressed using the EasyXpress Insect Kit II?
FAQ ID -1227
View
Can RNAprotect Saliva Reagent be purchased separately?
FAQ ID -1214
View
Can you recommend a Reagent or Kit for stabilizing RNA in cultured cells?
FAQ ID -1215
View
Can the Strep-Tactin Magnetic Beads of the Qproteome Plasma Membrane Protein Kit be reused?
FAQ ID -1204
View
Can the Qproteome Plasma Membrane Protein Kit be used with tissue samples?
FAQ ID -1200
View
Can the RLT lysate of the BioSprint DNA Plant protocol be stored frozen?
FAQ ID -1248
View
How can I decontaminate the BioRobot EZ1?
FAQ ID -1231
View
How do you ensure that concentrated RNA is purified from saliva using the RNeasy Protect Saliva Mini Kit?
FAQ ID -1212
View
How much active protein can be expressed in-vitro using the EasyXpress Insect Kit II, and how much time does it take?
FAQ ID -1222
View
How many freeze-thaw cycles can the various reaction mixes in the EasyXpress Insect Kit II tolerate?
FAQ ID -1219
View
Do you have a protocol for the isolation of DNA from buccal cells using the BioRobot EZ1?
FAQ ID -1260
View
Do you have a protocol for transfection of differentiated macrophage cell lines (THP) with siRNA?
-1
View
How are plasma membrane containing vesicles eluted off the ligand in the Qproteome Plasma Membrane Protein procedure?
FAQ ID -1203
View
Which fixation method is used in the PAXgene Tissue System?
FAQ ID - 3600
View
What is the fixation reagent of PAXgene Tissue FIX?
FAQ ID - 3601
View
What is the stabilization reagent of PAXgene Tissue STABILIZER?
FAQ ID - 3602
View
What is the maximum tissue size that can be fixed in a PAXgene Tissue FIX Container (50 ml)?
FAQ ID - 3604
View
Why are two reagents used in the PAXgene Tissue System?
FAQ ID - 3603
View
Which conditions are recommended for the storage of tissues in PAXgene Tissue STABILIZER?
FAQ ID - 3605
View
Is it possible to process formalin-fixed and PAXgene Tissue fixed samples together in one run?
FAQ ID - 3606
View
Is it necessary to clean a processor normally used for formalin-fixed tissue before using it with PAXgene Tissue fixed tissue?
FAQ ID - 3607
View
Is it possible to integrate the PAXgene Tissue STABILIZER into tissue processing?
FAQ ID - 3608
View
Are immunohistochemistry (IHC) assays developed for formalin-fixed, paraffin-embedded (FFPE) tissues compatible with PAXgene Tissue fixed, paraffin-embedded (PFPE) tissues?
FAQ ID - 3609
View
How well is RNA integrity preserved in PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?
FAQ ID - 3611
View
Is it possible to use formalin-fixed, paraffin-embedded (FFPE) kits and protocols to isolate biomolecules from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?
FAQ ID - 3610
View
How well is DNA integrity preserved in PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?
FAQ ID - 3612
View
Are special kits and protocols required to isolate biomolecules from PAXgene Tissue fixed, cryo-embedded (PFCE) tissue?
FAQ ID - 3614
View
What are the yield and integrity of nucleic acids isolated from blocks of PAXgene Tissue fixed, cryo-embedded (PFCE) tissue?
FAQ ID - 3613
View
Can proteins be extracted from PAXgene Tissue fixed specimens?
FAQ ID - 3616
View
Does the PAXgene Tissue RNA Kit co-purify small RNAs?
FAQ ID - 3615
View
Which kits and protocols can be used to isolate nucleic acids from microdissected PAXgene Tissue fixed, paraffin-embedded (PFPE) and PAXgene Tissue fixed, cryo-embedded (PFCE) specimens?
FAQ ID - 3617
View
What is the PCR performance of DNA purified from PAXgene Tissue fixed, paraffin-embedded (PFPE) and PAXgene Tissue fixed, cryo-embedded (PFCE) tissue compared to DNA from snap frozen or formalin-fixed, paraffin-embedded (FFPE) tissue?
FAQ ID - 3618
View
How is the quality of proteins purified from PAXgene Tissue fixed and stabilized tissue?
FAQ ID - 3619
View
Can the RNeasy kits be used to extract RNA from saliva collected using the Oragene collection kit?
FAQ ID - 3623
View
How do I analyze codon 61 mutations with the RAS extension kit (cat. no. 971590)?
FAQ ID - 3622
View
Are QR codes recognized by QIAsymphony?
FAQ ID - 3625
View
Do I need to use a blood collection set with the PAXgene Blood ccfDNA Tube?
FAQ ID - 3626
View
What is the reagent in the PAXgene Blood ccfDNA Tube?
FAQ ID - 3632
View
Does the insert sequence provided in the specification sheet correspond to the target sequence or to anti-sense sequence?
FAQ ID - 3627
View
What is the shelf life of unused PAXgene Blood ccfDNA Tubes?
FAQ ID - 3633
View
How should unused PAXgene Blood ccfDNA Tubes be stored?
FAQ ID - 3634
View
Are there special considerations for phlebotomy when using the PAXgene Blood ccfDNA Tube (blood collection set, discard tube, positioning of the tube, issues with short draws, etc.)?
FAQ ID - 3635
View
Are PAXgene Blood ccfDNA Tubes sterile?
FAQ ID - 3636
View
What is the expected ccfDNA yield from plasma generated from 10 ml of blood?
FAQ ID - 3637
View
Can I use plasma from underfilled PAXgene Blood ccfDNA Tubes?
FAQ ID - 3638
View
How long can whole blood stabilized in PAXgene Blood ccfDNA Tubes be stored at room temperature?
FAQ ID - 3639
View
Can whole blood in PAXgene Blood ccfDNA Tubes be stored at temperatures above 25°C?
FAQ ID - 3640
View
Can whole blood in PAXgene Blood ccfDNA Tubes be stored at temperatures below 15°C?
FAQ ID - 3641
View
Can whole blood be frozen in PAXgene Blood ccfDNA Tubes?
FAQ ID - 3642
View
Is double centrifugation required for plasma processing?
FAQ ID - 3643
View
How long and at which temperature can plasma generated from whole blood in PAXgene Blood ccfDNA Tubes be stored?
FAQ ID - 3644
View
What should I do if cryoprecipitates form while thawing a frozen plasma sample?
FAQ ID - 3646
View
Which plasma volumes can I process with the QIAsymphony PAXgene Blood ccfDNA protocols?
FAQ ID - 3648
View
How many extractions can I perform with a QIAsymphony PAXgene Blood ccfDNA Kit, using the 2.4 ml or the 4.8 ml protocols?
FAQ ID - 3649
View
How long after first use can a reagent cartridge be stored for further use?
FAQ ID - 3650
View
For what type of sample material is the QIAsymphony PAXgene Blood ccfDNA Kit intended?
FAQ ID - 3652
View
Which tubes are recommended for the QIAsymphony sample rack
FAQ ID - 3653
View
Which protocol line should I use to process plasma samples?
FAQ ID - 3654
View
Does the QIAsymphony PAXgene Blood ccfDNA Kit also allow eluting ccfDNA in less than 60 µl?
FAQ ID - 3656
View
How should eluates produced with the PAXgene Blood ccfDNA System be stored?
FAQ ID - 3657
View
Which downstream applications have been tested with ccfDNA purified from whole blood collected and processed with the PAXgene Blood ccfDNA System?
FAQ ID - 3658
View
What is the shelf life of the QIAsymphony PAXgene Blood ccfDNA Kit?
FAQ ID - 3651
View
Which elution volumes are available with the QIAsymphony PAXgene Blood ccfDNA Kit?
FAQ ID - 3655
View
Why is a blood collection set required to collect blood into the PAXgene Blood ccfDNA Tube?
FAQ ID - 3628
View
Can reagent from the PAXgene Blood ccfDNA Tube flow back into the patient’s arm?
FAQ ID - 3629
View
Can I use a different brand blood collection set with the PAXgene Blood ccfDNA Tube?
FAQ ID - 3630
View
What is the blood draw volume of the PAXgene Blood ccfDNA Tube
FAQ ID - 3631
View
What is the difference between RT² Profiler PCR Array and RT² Profiler PCR Array PLUS version?
FAQ ID - 3685
View
Where can I find the gene list for a specific RT² Profiler PCR Array?
FAQ ID - 3684
View
Is it possible to store nucleic acids extracted using the SpeedXtract Virus Kit?
FAQ ID - 3680
View
Total RNA from what sample types are compatible with the QIAseq miRNA Library Kit?
FAQ ID - 3660
View
Can miRNA sequencing libraries be prepared from total RNA isolated from heparinized plasma samples?
FAQ ID - 3661
View
Are any components of the QIAseq miRNA Library Kit interchangeable with components from other QIAGEN kits?
FAQ ID - 3662
View
Can I still perform sequencing if my miRNA sequencing library concentrations are too low to obtain a 4 nM library?
FAQ ID - 3666
View
What method is used to eliminate adapter dimers from the QIAseq miRNA library?
FAQ ID - 3667
View
Does a QIAseq miRNA library include hY4 Y RNA?
FAQ ID - 3670
View
What is the sequence of the QIAseq miRNA NGS 3’ Adapter?
FAQ ID - 3671
View
Do the covered regions in the BED file include the primers?
FAQ ID - 3689
View
What do the GTIN, UDI and REF abbreviations mean on the label of any QIAGEN box?
FAQ ID - 3697
View
Do eluates generated with the PAXgene Blood miRNA Kit contain residual genomic DNA?
FAQ ID - 3637
View
Do you have a protocol for high-throughput purification of plasmid DNA using the BioRobot Universal System?
FAQ ID -1191
View
Is the surface tension of the crystallization supports of the EasyXtal 15-Well Tools similar to that of a siliconized cover slide?
FAQ ID -1107
View
Is the pH indicated in the formulation sheet for Screening Suite buffers the final pH?
FAQ ID -1123
View
What are the applications the EasyXtal 15-Well Tool can be used for?
FAQ ID -1111
View
Is the EasyXtal Tool compatible with liquid-handling systems and visualization robots?
FAQ ID -1118
View
Is it possible to collect intact mitochondria before or after the density gradient centrifugation step of the Qproteome Mitochondria Isolation protocol?
FAQ ID -1187
View
What cell lines have been tested with the Fastlane Cell cDNA Kit?
FAQ ID -1175
View
What does 'QIAGEN Certified Solution' for Protein Crystallization mean?
FAQ ID -1121
View
How many protein crystallization conditions are available at QIAGEN?
FAQ ID -1119
View
How much detection reagent do you generally recommend for LiquiChip Assays?
FAQ ID -1159
View
How many cells can be used with the Qproteome Mitochondria Isolation Kit, and how much mitochondrial protein can be expected?
FAQ ID -1189
View
How many drops can be set up on a protein crystallization support?
FAQ ID -1110
View
How many LiquiChip beads are recommended for an xMAP experiment?
FAQ ID -1161
View
How much solution can I add to an EasyXtal 15-Well Tool reservoir well?
FAQ ID -1114
View
In which formats are crystallization condition Screening Suites available?
FAQ ID -1127
View
If I cannot find a QuantiTect Primer Assay for my target gene in rat, can I use the assays for the orthologous genes in mouse and human?
FAQ ID -1145
View
If a QuantiTect Primer Assay has more than one version number, does this mean that earlier assay versions may not work?
FAQ ID -1136
View
How sensitive is the LiquiChip Reader?
FAQ ID -1157
View
How much time is needed for a LiquiChip assay point measurement?
FAQ ID -1162
View
Why are there various different QuantiTect Primer Assays for my gene (previous versions, transcript variants)?
FAQ ID -1133
View
What is the delivery time for QuantiTect Primer Assays?
FAQ ID -1142
View
Where can I find the composition tables for solutions used in individual Crystallization Screening Suites?
FAQ ID -1124
View
What kind of strategy for initial screening of protein crystallization conditions do you recommend?
FAQ ID -1120
View
When searching for a QuantiTect Primer Assay for my gene of interest, why do I only find the assay for human but not for rat?
FAQ ID -1139
View
What type of magnet do you recommend for handling the magnetic beads in the Qproteome Plasma Membrane Protein protocol?
FAQ ID -1193
View
What starting material can be used with the Qproteome Mitochondria Isolation Kit?
FAQ ID -1184
View
Can the EZ1 DNA Investigator Tip Dance protocol cope with any solid matrix remaining in the sample tube?
FAQ ID -1153
View
Are EasyXtal 15-Well Tools stackable?
FAQ ID -1116
View
Why can I not find the QuantiTect Primer Assay in GeneGlobe that I had previously ordered from QIAGEN?
FAQ ID -1138
View
Which QuantiTect Primer Assay should I choose for my gene of interest?
FAQ ID -1135
View
Why does the QuantiTect Primer Assay for my gene of interest have only one version number?
FAQ ID -1134
View
Which chemical products do you use to prepare screening solutions for protein crystallization?
FAQ ID -1122
View
Will proteins eluted in the Qproteome Plasma Membrane Protein protocol be modified, e.g. with the ligand?
FAQ ID -1195
View
Will the ligand in the Qproteome Plasma Membrane Protein protocol be specific for plasma membrane exclusively?
FAQ ID -1198
View
Will integral, peripheral as well as lipid-anchored plasma membrane proteins be isolated with the Qproteome Plasma Membrane Protein Kit?
FAQ ID -1199
View
Why is there no QuantiTect Primer Assay for my gene of interest?
FAQ ID -1140
View
What is the EasyXtal 15-Well Tool made of?
FAQ ID -1106
View
What is the dynamic range of detection of the LiquiChip Reader?
FAQ ID -1158
View
Can the nuclei and cell debris fraction generated in the QProteome Mitochondria isolation procedure be used for further fractionation?
FAQ ID -1185
View
Can the EZ1 DNA Forensic Card be used with the EZ1 DNA Investigator Kit?
FAQ ID -1151
View
Can the EasyXtal 15-Well Tools be reused?
FAQ ID -1117
View
Can the Qproteome Plasma Membrane Protein Kit be used with suspension cells?
FAQ ID -1194
View
Can your Tag antibody recognize rat MAPK1 also?
-100
View
Can xMAP kits from other Luminex partners be used on the LiquiChip System purchased from QIAGEN?
FAQ ID -1156
View
Can the remaining fractions obtained in the Qproteome Plasma Membrane Protein procedure be used in downstream assays?
FAQ ID -1197
View
Can the Spin Columns of the Qproteome Albumin/IgG Depletion Kit be reused?
FAQ ID -1173
View
Are scoring sheets available for each crystallization condition Screening Suite?
FAQ ID -1125
View
Can mitochondria separated with the Qproteome Mitochondria Isolation Kit be used for downstream DNA isolation?
FAQ ID -1188
View
Are protein modifications such as glycosylation problematic when using the Qproteome Plasma Membrane Protein Kit?
FAQ ID -1196
View
Can acetone be used for precipitation of protein from Buffer RLT lysates generated with RNeasy Kits?
FAQ ID -1164
View
Can I increase the annealing temperature recommended for QuantiTect Primer Assays used with the QuantiTect SYBR Green PCR Kits?
FAQ ID -1146
View
How are NeXtal deep-well blocks sealed? Can they be resealed?
FAQ ID -1128
View
Does the EasyXtal Tool conform to an SBS format?
FAQ ID -1115
View
Do you have WGA protocols for starting materials not mentioned in the REPLI-g Handbooks?
FAQ ID -1160
View
How easy is it to scoop up a protein crystal with the lip around the crystallization supports of the EasyXtal 15-Well Tools?
FAQ ID -1113
View
Do you have a protocol for the purification of 6xHis-tagged proteins using BioSprint?
FAQ ID -1167
View
How did you determine that your AllStar Negative Control siRNA binds to RISC?
FAQ ID -1180
View
How is the quality of the crystallization condition Screening Suites guaranteed?
FAQ ID -1126
View
How do I use EasyXtal Crystallization Tools?
FAQ ID -1105
View
How does the sealing efficiency of the crystallization supports of the EasyXtal 15-Well Tool compare to grease?
FAQ ID -1109
View
Do you have a kit for protein isolation from mitochondria prepared with your Qproteome Mitochondria Isolation Kit?
FAQ ID -1190
View
Do you have a kit for the isolation of RNA from formalin-fixed, paraffin-embedded samples?
FAQ ID -1174
View
Do I need an ultracentrifuge for the density gradient centrifugation step in the Qproteome Mitochondria Isolation protocol?
FAQ ID -1186
View
Do protein crystals stick to the walls of cavities when using the DropGuard crystallization supports?
FAQ ID -1108
View
Do you have a protocol for evaluating pipetting accuracy of the BioRobot EZ1?
FAQ ID -1131
View
Do you have a protocol for purification of DNA from stool samples for pathogen detection using BioSprint Instruments?
FAQ ID -1165
View
Do you have a protocol for purification of DNA from stool samples using BioSprint Instruments?
FAQ ID -1166
View
Do you have a protocol for evaluating temperature accuracy of the BioRobot EZ1?
FAQ ID -1168
View
Which competent E. coli cells should I use to amplify the SureSilencing shRNA Plasmids?
FAQ ID -2890
View
Which analyses can be performed with the PyroMark Q96 ID software version 1.0?
FAQ ID -2845
View
Does the same U1 promoter in the SureSilencing shRNA Plasmids express shRNA in human, mouse, and rat cell lines?
FAQ ID -2887
View
How can I use the SureSilencing shRNA Plasmids for stable transfection and knock down in a cell line that is already resistant to both neomycin and puromycin?
FAQ ID -2895
View
Can I use my own siRNA design?
FAQ ID -2897
View
Can I order the nucleotides from PyroMark Gold Reagents separately?
FAQ ID -2827
View
Can I reclone the insert sequences from the SureSilencing shRNA Plasmids into a viral-based vector?
FAQ ID -2896
View
Can I directly transfect SureSilencing shRNA Plasmids into my cells?
FAQ ID -2899
View
Do any of the SureSilencing shRNA Plasmids contain inducible promoters?
FAQ ID -2888
View
Can PyroMark Gold reagents be vortexed?
FAQ ID -2844
View
What are the features of the new PyroMark Q96 ID software 2.5?
FAQ ID -2867
View
What cell line does QIAGEN use for quality control testing of the SureSilencing shRNA Plasmids?
FAQ ID -2885
View
Should I use the SureSilencing shRNA Plasmids with the neomycin-resistance marker, the puromycin-resistance marker, or the GFP reporter gene?
FAQ ID -2882
View
What else do I need to complete an experiment using the SureSilencing shRNA Plasmids?
FAQ ID -2889
View
What cell lines can I use with the SureSilencing shRNA Plasmids?
FAQ ID -2891
View
How long should I wait after transfection before enriching my transfected cells, validating knock down, and looking for phenotypic changes?
FAQ ID -2893
View
Is the expression level of miRNA precursors lower compared to that of mature miRNAs?
FAQ ID -2807
View
How many times can the CDTs, NDTs, and RDTs be used?
FAQ ID -2865
View
What is the PyroMark Q96 Data Converter?
FAQ ID -2868
View
What is the pyrosequencing data exchange tool for?
FAQ ID -2864
View
What is the composition of Buffer APP?
FAQ ID -2804
View
What is the composition of Buffer FRN?
FAQ ID -2802
View
What is the composition of Buffer APL?
FAQ ID -2803
View
Which analyses can be performed with the PyroMark Q96 MD software?
FAQ ID -2866
View
Which PyroMark Gold Q96 Reagent should be used for which instrument and application?
FAQ ID -2836
View
What is the SureSilencing shRNA Plasmids guarantee?
FAQ ID -2898
View
What primers may I use to sequence the shRNA cassette within the SureSilencing shRNA Plasmids?
FAQ ID -2886
View
Does the PyroMark CpG LINE assay target mouse transposons as well?
-1
View
How do I find the SureSilencing shRNA Plasmids for my gene of interest?
FAQ ID -2883
View
How are the SureSilencing shRNA Plasmids shipped?
FAQ ID -2884
View
Which sample volumes can be used for the QIAsymphony DSP Circulating DNA Kit?
FAQ ID - 3700
View
What sample volume should be loaded on the QIAsymphony instrument for extraction of 2 ml and 4 ml samples?
FAQ ID - 3701
View
Which elution volume is used for the QIAsymphony DSP Circulating DNA Kit?
FAQ ID - 3703
View
How much circulating cell-free DNA can I expect from samples, and how can I quantify the yield?
FAQ ID - 3704
View
How can I check the quality or size of my extracted circulating cell-free DNA?
FAQ ID - 3705
View
How long can a reagent cartridge remain open without affecting my results?
FAQ ID - 3706
View
What is the concentration of SureENTRY Transduction Reagent?
FAQ ID - 3708
View
Is it possible to get a Custom RT2 Profiler PCR Array, cat. # 330131, with primer assays targeting transcripts of different species, e.g. assays targeting transcripts from mouse and assays targeting transcripts from human, on one plate?
FAQ ID - 3710
View
Is it possible to purchase Buffer VXL which is part of the QIAamp cador Pathogen Mini Kit, (Cat No.: 54104, 54106) separately?
FAQ ID - 3712
View
What sample tubes can I use on my QIAsymphony SP system?
FAQ ID - 3713
View
How can I decontaminate my QIAsymphony SP / AS system?
FAQ ID - 3714
View
Can other QIAGEN buffers from non-UCP kits (e.g. AW1 instead of AUW1) be used with the QIAamp UCP DNA Micro Kit (cat. no. 56204)?
FAQ ID - 3717
View
How is the Pyromark Q48 cartridge to be cleaned?
FAQ ID - 3718
View
Can the disc and absorber strips for the Pyromark Q48 be reused?
FAQ ID - 3720
View
What is the dead volume of the cartridges in PyroMark Q48?
FAQ ID - 3719
View
How much DNA is needed in the PyroMark Q48?
FAQ ID - 3721
View
Can I use PyroMark Q48 for diagnostics?
FAQ ID - 3722
View
Can I transfer my assays from PyroMark Q24/Q96 to PyroMark Q48 Autoprep?
FAQ ID - 3723
View
Can I analyze my Next Generation Sequencing (NGS) samples on the QIAxcel for Quality control?
FAQ ID - 3724
View
Is an additional proteinase K digest possible with DNeasy PowerSoil Kit and DNeasy PowerLyzer Kit?
FAQ ID - 3725
View
Can I buy the 1 ml Bead Plates from the DNeasy UltraClean 96 Microbial Kit (384) separately?
FAQ ID - 3726
View
Does QIAGEN offer an anti-GFP gapmer control?
FAQ ID - 3739
View
Why do I observe a CT shift for the Internal Control in some samples in comparison to the CT value for the Internal Control of the dilution series of the Male Control DNA M1?
FAQ ID - 3745
View
How long can I store the dilutions of the Male Control DNA M1 standards at 2–8°C?
FAQ ID - 3744
View
Do I need to buy special kits for the QIAcube Connect?
FAQ ID - 141485
View
Can I program my own protocols for the QIAcube Connect?
FAQ ID - 141486
View
Can the QIAcube Connect rotor and/or buckets be removed for cleaning?
FAQ ID - 141491
View
Can purification columns from other suppliers be processed on the QIAcube Connect?
FAQ ID - 141487
View
How can I get software updates for the QIAcube Connect?
FAQ ID - 141494
View
Is it necessary to place the lids of the elution tube and column into the slots of the rotor adapter during processing on the QIAcube Connect?
FAQ ID - 141496
View
Do I need to discard partially used QIAcube Connect tip racks?
FAQ ID - 141499
View
How can I decontaminate the QIAcube Connect system?
FAQ ID - 141500
View
Can the QIAcube Connect heater/shaker be used independently from protocol runs?
FAQ ID - 141502
View
How can I load new protocols onto the QIAcube Connect?
FAQ ID - 141501
View
What dedicated QIAcube Connect kits are available?
FAQ ID - 141504
View
Do you have a protocol for the QIAprep Spin M13 Kit on the QIAcube Connect?
FAQ ID - 1414507
View
Where can I find the ordering information for QIAcube Connect accessories such as shaker rack plugs, rack labeling strips, reagent bottle racks, 30 ml reagent bottles, rotor-adapter holder, rotor adapters, sample tubes CB, sample tubes RB and spin-column adapter rings?
FAQ ID - 141505
View
Do you have a protocol for the AllPrep DNA/RNA/Protein Mini Kit on the QIAcube Connect?
FAQ ID - 141506
View
Which QIAcube Connect standard protocol might be suitable to extract RNA from saliva or from a buccal cell pellet?
FAQ ID - 141508
View
How often should the o-ring for the pipettor tip adapter be changed on the QIAcube Connect?
FAQ ID - 141509
View
Can the CB and RB sample tubes be used interchangeably?
FAQ ID - 141510
View
Which network settings are supported by the QIAcube Connect and Q-Base?
FAQ ID - 141517
View
Why can I not find the Q-Base device in the device list during the first installation?
FAQ ID - 141516
View
Why can’t I see my QIAcube Connect instrument in the QIAcube Connect App?
FAQ ID - 1414518
View
Why is the QIAcube Connect App reacting so slowly?
FAQ ID - 141519
View
Why are not all applications/kits/protocol files visible in the run setup (QIAcube Connect App)?
FAQ ID - 1414520
View
Why does the connection test fail?
FAQ ID - 141522
View
How can I accurately determine the amount of input material for the EpiTect Hi-C Kit?
FAQ ID -
143065
View
What sample source do you support?
FAQ ID -
143066
View
How long does it take to complete the EpiTect Hi-C protocol?
FAQ ID -
143068
View
How many reactions are in a kit?
FAQ ID -
143067
View
Can I use cells that have been previously crosslinked and frozen (e.g., for a ChIP or ChIP-seq experiment) as input material for the EpiTect Hi-C protocol?
FAQ ID -
143069
View
Can I substitute the endonuclease used in the Hi-C Digestion step with another endonuclease?
FAQ ID -
143070
View
Are there stopping points in the Hi-C workflow?
FAQ ID -
143071
View
With which platform can I perform my sequencing?
FAQ ID -
143072
View
With which read length should the EpiTect Hi-C NGS libraries be sequenced
FAQ ID -
143073
View
How do I know if my Hi-C sample is a good candidate for deep sequencing?
FAQ ID -
143074
View
What kind of analysis does the EpiTect Hi-C Data Analysis Portal perform?
FAQ ID -
143076
View
Can I use my own pipeline to analyze results from my EpiTect Hi-C experiments?
FAQ ID -
143078
View
How critical is the time of the temperature change from 60°C to 80°C?
FAQ ID -
143768
View
Is it possible to use Lyse&Spin baskets?
FAQ ID -
143762
View
Why is DTT used only for semen samples?
FAQ ID -
143761
View
Can the Repli-g Advanced DNA Single Cell Kit also be used for bacterial cells?
FAQ ID -
143079
View
Can the lysis volume be reduced for higher sensitivity?
FAQ ID -
143763
View
What if only one heater shaker is available?
FAQ ID -
143764
View
Why is there no male DNA obtained from sexual assault samples?
FAQ ID -
143767
View
If 400 µl are used as lysis volume, how do I concentrate the lysate? (Otherwise the DNA would be too diluted.)
FAQ ID -
143766
View
Why is the QIAamp Viral RNA Mini Kit (50) currently unavailable?
FAQ ID -147395
View
What RNAs has QIAseq FastSelect been designed to remove?
FAQ ID -147910
View
How does QIAseq FastSelect work?
FAQ ID -147907
View
Is QIAseq FastSelect truly as easy as combining a reagent with RNA and ramping down the temperature?
FAQ ID -147909
View
Will QIAseq FastSelect work in other species?
FAQ ID -147911
View
What library prep kits has QIAseq FastSelect been tested with?
FAQ ID -147912
View
I am using an RNA library prep kit that is not listed in the QIAseq FastSelect handbook. Will QIAseq FastSelect work with my kit?
FAQ ID -147913
View
What RNA range has the QIAseq FastSelect been tested with?
FAQ ID -147914
View
Can QIAseq FastSelect Plant or QIAseq FastSelect Yeast be combined with FastSelect 5S/16S/23S?
FAQ ID -147915
View
Is it possible to test the efficiency of FastSelect rRNA removal by using a Bioanalyzer, etc.?
FAQ ID -147916
View
Is plasmid DNA purified with QIAGEN Plasmid Purification Kits suitable for in vitro transcription?
FAQ ID -1
View
How should I quantify RNA isolated with RNeasy Kits?
FAQ ID -32
View
When is chloramphenicol amplification of plasmids performed?
FAQ ID -3
View
What are the expected DNA yields from different tissues using the QIAamp DNA Mini Kit?
FAQ ID -45
View
What are the most commonly used protease inhibitors?
FAQ ID -53
View
How should I propagate pQE expression plasmids?
FAQ ID -58
View
How can I increase the amount of soluble recombinant protein in E. coli expression?
FAQ ID -64
View
How can I remove imidazole from a protein sample?
FAQ ID -91
View
Why does my isolated RNA have a low OD 260/280 ratio?
FAQ ID -97
View
How can I avoid little or no RNA yields when using an RNeasy Kit?
FAQ ID -28
View
Do I need to use an RNase inhibitor in my RT reaction?
FAQ ID -119
View
Can I use QIAprep Miniprep kits for low-copy plasmids and cosmids?
FAQ ID -127
View
Can I use the QIAquick PCR Purification Kit for restriction enzyme cleanup?
FAQ ID -130
View
How does imidazole affect my quantitation of protein?
FAQ ID -132
View
Can a QIAquick Gel Extraction Kit be used to obtain RNA from a formaldehyde gel?
FAQ ID -133
View
What is the difference between disruption and homogenization in the RNeasy System?
FAQ ID -139
View
What is the size of genomic DNA that is obtained with QIAGEN Genomic-tips?
FAQ ID -142
View
Should I use Ni-NTA Agarose in column or batch format for purification of 6xHis-tagged proteins?
FAQ ID -147
View
What is the genotype of the EZ Competent Cells?
FAQ ID -157
View
What are the restriction sites of the pDrive Vector in the QIAGEN PCR Cloning Kit?
FAQ ID -160
View
What are the molecular weights of proteins in the 6xHis Protein Ladder?
FAQ ID -169
View
How can I optimize the transfection of oligos and large plasmids using PolyFect Transfection Reagent?
FAQ ID -176
View
How can I improve recoveries when using the QIAquick Kits?
FAQ ID -180
View
How can I improve transfection efficiency using Effectene Transfection Reagent?
FAQ ID -181
View
What is the principle behind Effectene Transfection Reagent?
FAQ ID -184
View
What is the composition of Buffer EB?
FAQ ID -199
View
Is it possible to modify the transduction protocol for Cignal Lenti Reporter Assays to save some pipetting steps?
FAQ ID - 3709
View
Can I store the QIAseq Beads of the QIAseq Targeted DNA Panel at -20°C?
FAQ ID - 3711
View
What is the difference between the QIAamp UCP DNA Micro Kit (cat. no. 56204) and RNeasy UCP Kits (cat. no. 73934) and non-UCP QIAGEN purification kits?
FAQ ID - 3715
View
Can I use carrier RNA with the RNeasy UCP Micro Kit (cat. no. 73934)?
FAQ ID - 3716
View
How can I check for purity of RNA isolated using RNeasy Kits?
FAQ ID -1023
View
What is the recipe for 1x PBS solution?
FAQ ID -1030
View
Where can I find a protocol for cleanup of already purified plasmid DNA?
FAQ ID -1031
View
How do I perform an Acetone Precipitation for concentrating and desalting protein samples?
FAQ ID -1035
View
What is the difference between Buffers RLT and RLT Plus?
FAQ ID -1043
View
What is the largest PCR amplicon that can be amplified with the HotStar HiFidelity Polymerase Kit?
FAQ ID -1047
View
Are the CoralLoad dyes in the HotStarTaq Plus PCR Buffer visible when loading small amounts of PCR product onto a gel?
FAQ ID -1051
View
Do I have to use CoralLoad Gel loading dye when using your HotStarTaq Plus DNA Polymerase?
FAQ ID -1052
View
How can I increase DNA concentration using QIAprecipitators of the HiSpeed Plasmid Kits?
FAQ ID -1061
View
What should I use as a standard for absolute quantification in real-time PCR?
FAQ ID -1085
View
What does transcript variant mean when searching for specific QuantiTect Primer Assays?
FAQ ID -1137
View
What is a QuantiTect Primer Assay?
FAQ ID -1141
View
Do QuantiTect Primer Assays contain SYBR Green dye?
FAQ ID -1143
View
What is the smallest and the largest protein that you tested for expression with the EasyXpress Insect Kit II?
FAQ ID -1218
View
What are the new features of the EasyXpress Insect Kit II compared to the original Protein Synthesis Insect kit?
FAQ ID -1220
View
What are your recommendations for PCR template preparation for use with the EasyXpress Insect Kit II?
FAQ ID -1221
View
Do you have a protocol for transfection of suspension cell lines (Jurkat and K562) with siRNA?
FAQ ID -1250
View
Do you have a protocol for transfection of macrophage cell lines (J774.A1 and RAW 264.7) with siRNA?
FAQ ID -1251
View
What is the pH value of QIAGEN's Nuclease-Free Water?
FAQ ID -1290
View
Why is there DNA in the no-template control reaction when using the standard REPLI-g procedure, but not when using the UltraFast procedure?
FAQ ID -1327
View
Will the REPLI-g UltraFast Mini Kit reaction always yield 10 µg DNA?
FAQ ID -1328
View
Can the bacterial pellet in the QuickLyse Miniprep procedure be resuspended by pipetting up and down or shortening the vortexing time?
FAQ ID -1352
View
How can I increase the DNA yield from low-copy plasmids using the QuickLyse Miniprep Kit?
FAQ ID -1354
View
What is the composition of elution buffer QLE in the QuickLyse Miniprep Kit?
FAQ ID -1356
View
How do I resuspend my FlexiPlate siRNAs?
FAQ ID -1360
View
Are there any specific cleaning solutions recommended for the QIAcube?
FAQ ID -1416
View
Why is a 2-step (and not a 3-step) cycling protocol recommended for QuantiFast SYBR Green PCR Kits?
FAQ ID -1450
View
Can tissue homogenized in Buffer RLT Plus instead of Buffer RLT be used with the AllPrep DNA/RNA/Protein Mini Kit?
FAQ ID -1577
View
What can be done if the protein pellet does not solubilize completely using the AllPrep DNA/RNA/Protein Mini Kit?
FAQ ID -1580
View
What is the binding capacity of the Ni-NTA Superflow Cartridges?
FAQ ID -1603
View
How comparable is CoralLoad gel loading dye contained in various QIAGEN PCR Kits to Sigma Red?
FAQ ID -1644
View
Do I need to resuspend FlexiTube siRNAs in siRNA Resuspension buffer?
FAQ ID -1659
View
Are FlexiTube siRNA and FlexiTube GeneSolution also available for rat siRNAs?
FAQ ID -1664
View
What is the composition of Buffer PE?
FAQ ID -1691
View
What is the upper length of PCR products that can be amplified with TopTaq DNA Polymerase and Master Mix Kits?
FAQ ID -1738
View
What is the minimum sample volume required for the QIAxcel System and QIAxcel Advanced?
FAQ ID -1852
View
What are the dimensions and weight of the QIAsymphony SP System?
FAQ ID -1901
View
Are there any specific cleaning solutions recommended for the QIAsymphony SP?
FAQ ID -1913
View
How much sample can be used with the RNeasy Plus 96 Kit?
FAQ ID -1992
View
What is the composition of Buffer P2?
FAQ ID -203
View
A white precipitate has formed in my 10x RT buffer. Is it still ok to use?
FAQ ID -216
View
For how long are BioMag Particles stable?
FAQ ID -262
View
I used to buy BioMag particles from a company by another name? Are your BioMag particles the same thing?
FAQ ID -263
View
Can I clean up my DNase treated RNA samples using RNeasy columns?
FAQ ID -286
View
How can one determine the optimal annealing temperature for a specific PCR assay?
FAQ ID -288
View
What is the composition of the QIAGEN Multiplex PCR Buffer?
FAQ ID -289
View
Can I use HEPES buffer instead of phosphate in my Ni-NTA column?
FAQ ID -291
View
Can I use a centrifuge instead of vacuum when using the QIAquick 96 PCR Purification Kit?
FAQ ID -293
View
What are the ideal and the largest PCR amplicon sizes when using the EpiTect MSP Kit?
FAQ ID -2004
View
What is elution Buffer ATE of the QIAsymphony DNA Investigator Kit?
FAQ ID -2029
View
How can a C-terminal 6xHis-tagged QIAgene be expressed?
FAQ ID -2041
View
Why is there more than one QIAgene for my gene of interest?
FAQ ID -2044
View
Is a passive internal reference dye, like ROX, required on the Rotor-Gene Q to obtain reproducible results?
FAQ ID -2079
View
Does the Rotor-Gene Q software have the capacity to export data?
FAQ ID -2089
View
Why is a 2-step (and not a 3-step) cycling protocol recommended for Rotor-Gene SYBR Green Kits?
FAQ ID -2122
View
Does carrier RNA have to be added using the QIAsymphony Virus Blood 200 Protocol?
FAQ ID -2163
View
Can I use the RNeasy Microarray Tissue Mini Kit for all tissue types?
FAQ ID -2190
View
How is the RNeasy Microarray Tissue Mini Kit different from the classic RNeasy Mini Kit?
FAQ ID -2192
View
Why is EvaGreen instead of SYBR Green used as fluorescent dye in the Type-it HRM PCR Kit?
FAQ ID -2196
View
Why do I need normalization using Rotor-Gene ScreenClust HRM Software?
FAQ ID -2198
View
What is the difference between probability and typicality in the Rotor-Gene ScreenClust HRM Software?
FAQ ID -2203
View
What buffer conditions give the best resolution for agarose gel electrophoresis?
FAQ ID -2256
View
How much DNA should be loaded per well of an agarose gel?
FAQ ID -2259
View
Why do I have wavy DNA bands on my agarose gel?
FAQ ID -2260
View
What is the "seed sequence" of a miRNA?
FAQ ID -2262
View
Are you able to supply a Certificate of Analysis for the KRAS and EGFR PCR kits?
FAQ ID -2301
View
Which Rotor-Gene Q rotor should be used with the EGFR RGQ PCR kit?
FAQ ID -2315
View
How is the RNeasy Plus Universal Kit different from the RNeasy Plus Kit?
FAQ ID -2340
View
How does the gDNA eliminator solution of the RNeasy Plus Universal Kit work?
FAQ ID -2341
View
How long can I store my purified DNA/RNA isolated with the AllPrep DNA/RNA FFPE kit?
FAQ ID -2350
View
Do you have any information or guidelines regarding the choice of reference genes for real-time PCR?
FAQ ID -2371
View
Can I buy QIAquick and MinElute columns separately?
FAQ ID -2460
View
What is the PAXgene 96 filter plate?
FAQ ID -2486
View
Is RNA from red blood cells also isolated when RNA is extracted from a PAXgene Bone Marrow RNA Tube?
FAQ ID -2509
View
Is the 260/230 nm absorbance ratio specified for RNA derived from the PAXgene Bone Marrow RNA System?
FAQ ID -2514
View
How long is the fixation time?
FAQ ID -2519
View
What is the nature of the heterologous Internal Amplification Control and Positive Control in the mericon Assay kits?
FAQ ID -2543
View
Are there any accessories required for sample disruption with the TissueLyser LT?
FAQ ID -2551
View
Why do the Internal Control templates for extraction (Internal Control DNA or RNA [High conc.]) have a 10x higher concentration than the IC templates provided with the QuantiFast Pathogen PCR +IC Kit and the QuantiFast Pathogen RT-PCR +IC Kit?
FAQ ID -2603
View
During data analysis, how should the threshold be set in the Yellow channel on the Rotor-Gene Q cycler to analyze the Internal Control from the QuantiFast Pathogen PCR +IC Kit or the QuantiFast Pathogen RT-PCR +IC Kit
FAQ ID -2607
View
What is the SBTengine® software?
FAQ ID -2628
View
How do I create a workspace that is free of DNA contamination, prior to carrying out a qPCR experiment?
FAQ ID -2654
View
What is the recommended solution in which to store RNA samples that will be used as templates for cDNA synthesis?
FAQ ID -2659
View
How can I avoid or remove genomic DNA contamination from the total RNA preparation?
FAQ ID -2662
View
What are the differences between one-step and two-step RT--PCR?
FAQ ID -2666
View
What is qPCR?
FAQ ID -2668
View
What are the common primer and probe chemistries utilized for qPCR assays?
FAQ ID -2669
View
What is the purpose of the ROX and fluorescein dyes, also known as passive reference dyes, in qPCR master mixes?
FAQ ID -2671
View
What negative controls are typically included in qPCR and/or qRT-PCR experiments?
FAQ ID -2672
View
What positive controls are typically included in qPCR and/or qRT-PCR experiments?
FAQ ID -2673
View
What are the guidelines for choosing a housekeeping gene for normalizing qPCR results?
FAQ ID -2674
View
Why is 18S ribosomal RNA (rRNA) used as a housekeeping gene to normalize sample-to-sample, systematic variation in qPCR assays?
FAQ ID -2675
View
How does HotStart PCR help minimize nonspecific amplification events?
FAQ ID -2676
View
What is a dissociation curve, and why is it important to run a dissociation curve, following qPCR using SYBR Green chemistry?
FAQ ID -2678
View
What is the delta Rn value?
FAQ ID -2681
View
What is the threshold cycle or Ct value?
FAQ ID -2682
View
Why are my qPCR Ct values too low (< 12) in my qRT-PCR Assay?
FAQ ID -2684
View
Why are my qPCR Ct values too high (> 35 or not detectable) in my qRT-PCR assay?
FAQ ID -2685
View
Why is my no template control (NTC) real-time Ct value < 35 cycles in my qPCR Assay?
FAQ ID -2686
View
Why do my qPCR amplification curves or plots decrease in fluorescence intensity after the saturation phase?
FAQ ID -2689
View
Why do I see multiple high-intensity peaks in my qPCR dissociation curve at temperatures less than 70 ºC?
FAQ ID -2690
View
What is the standard curve method for qPCR assay data analysis? How is the standard curve method for qPCR assay data analysis performed?
FAQ ID -2691
View
What is the difference between Absolute Quantification and Relative Quantification in qPCR, using the standard curve approach?
FAQ ID -2692
View
What is the comparative or ??Ct method for qPCR assay data analysis? How is the comparative or ??Ct method for qPCR assay data analysis performed?
FAQ ID -2693
View
How do I determine the amplification efficiency of my qPCR assay?
FAQ ID -2694
View
How do I determine the linear dynamic range of my qPCR or qRT-PCR assay?
FAQ ID -2696
View
What is the best approach for determining where to set the CT threshold when you have >15 samples? Is it best to go through all of them, looking for a range of best fit, and then just choose one value that fits all of them?
FAQ ID -2705
View
What do I need to complete a RT² qPCR Primer Assay?
FAQ ID -2707
View
How can I prevent the evaporation of reaction volume from the wells in the miScript miRNA PCR array?
FAQ ID -2729
View
What is the minimum amount of genomic DNA required for analysis using EpiTect Methyl qPCR Arrays?
FAQ ID -2739
View
How do you design your EpiTect ChIP qPCR Assay primers? Do they work with SB GR?
FAQ ID -2749
View
How can I identify the binding sites of my transcription factor using ChIP?
FAQ ID -2753
View
At what point can I stop and freeze my samples when using ChIP?
FAQ ID -2754
View
What is RNA interference (RNAi)?
FAQ ID -2755
View
What are the key factors for success in an RNAi experiment?
FAQ ID -2758
View
What criteria should one use in choosing between siRNA versus shRNA for their studies?
FAQ ID -2771
View
How does stable transfection of a shRNA-encoding plasmid work?
FAQ ID -2779
View
What are the SureSilencing shRNA Plasmids?
FAQ ID -2784
View
What is the composition of Buffer S3?
FAQ ID -2788
View
What is the composition of Buffer ETR?
FAQ ID -2789
View
What is the composition of Buffer BB?
FAQ ID -2790
View
What is the composition of Buffer RLT plus?
FAQ ID -2794
View
What is the composition of Buffer RLC?
FAQ ID -2795
View
What is the composition of Buffer RPE?
FAQ ID -2797
View
What is the composition of Buffer RWT?
FAQ ID -2798
View
What is the composition of gDNA Eliminator Solution?
FAQ ID -2799
View
What is the composition of Buffer RDD?
FAQ ID -2800
View
What is the composition of Buffer PKD?
FAQ ID -2801
View
What is the composition of Buffer ALO?
FAQ ID -2805
View
What is the composition of RBC Lysis Solution?
FAQ ID -2808
View
What is the composition of Cell Lysis Solution?
FAQ ID -2809
View
What is the composition of Protein Precipitation Solution?
FAQ ID -2810
View
What is the composition of Cell Suspension Solution?
FAQ ID -2811
View
What is the composition and concentration of Glycogen Solution in Gentra Puregene Kits?
FAQ ID -2812
View
What is the composition of DNA Hydration Solution in Gentra Puregene Kits?
FAQ ID -2813
View
What is the meaning of the abbreviations NDT and CDT and what are the differences?
FAQ ID -2841
View
What are the changes of PyroMark Assay Design Software Version 2.0 compared to 1.0?
FAQ ID -2849
View
Can I use fluorescence microscopy to assess the transfection efficiency of the SureSilencing shRNA Plasmids with GFP in my model cell line of interest?
FAQ ID -2894
View
Do tissue samples require pretreatment before loading onto the QIAsymphony SP for DNA extraction?
FAQ ID -2926
View
What is the composition of Buffer G2?
FAQ ID -2943
View
What is the composition of Buffer B1?
FAQ ID -2944
View
What is the composition of Buffer B2?
FAQ ID -2945
View
How much DNA does a human cell contain?
FAQ ID -2948
View
How much RNA does a bacterial cell contain?
FAQ ID -2949
View
How much DNA and RNA can be expected from human blood cells?
FAQ ID -2950
View
What is the cellular composition of human blood?
FAQ ID -2951
View
How can I desalt and concentrate a protein fraction by trichloroacetic acid (TCA) precipitation?
FAQ ID -2954
View
Is there a risk of crosstalk from the FAM channel to the VIC channel if the residual DNA target is present in very high copy numbers?
FAQ ID -2980
View
Why is the final heat denaturation step so important with the QIAsymphony PAXgene Blood RNA protocol?
FAQ ID -2987
View
What is the sequence of the miScript Universal Primer?
FAQ ID -2994
View
How can I keep my centrifuge tubes endotoxin-free?
FAQ ID -301
View
Can Buffers N3 and P3 be used interchangeably?
FAQ ID -310
View
Are QIAprep and QIAquick Spin columns interchangeable?
FAQ ID -311
View
Can I store agarose gel slices containing DNA for gel extraction at a later point?
FAQ ID -313
View
How can QIAGEN Protease and Proteinase K be inactivated?
FAQ ID -315
View
Does the 5x OneStep RT-PCR Buffer contain BSA?
FAQ ID -326
View
What is the origin of replication and the plasmid copy number of the pQE vectors?
FAQ ID -338
View
Is QIAGEN carrier RNA sold separately?
FAQ ID -351
View
What is the white insoluble precipitate in my resuspended plasmid DNA pellet?
FAQ ID -352
View
What is the composition of PBS?
FAQ ID -361
View
How can I express toxic protein in E. coli?
FAQ ID -373
View
How can I improve DNA yields from very tough tissues using the DNeasy Blood & Tissue Kit or the QIAamp DNA Mini Kit?
FAQ ID -374
View
What are the dissociation constants for the Anti-His Antibodies?
FAQ ID -385
View
What is the average molecular weight of a siRNA, and how do I convert uM to ug values?
FAQ ID -388
View
Can siRNA silence bacterial genes?
FAQ ID -395
View
How should I reconstitute the lyophilized RNase-Free DNase Set with the RNeasy 96 kit?
FAQ ID -3003
View
Is it possible to use a standard processor — the kind used routinely for formalin-fixed samples — for dehydration and paraffin infiltration of PAXgene Tissue treated samples?
FAQ ID -3032
View
Can I use Tungsten Carbide beads for RNA extraction protocols using QIAGEN Kits?
FAQ ID -3053
View
Where can I find the ordering information for QIAcube accessories such as Shaker Rack plugs, Rack labeling strips, Reagent bottle rack, 30mL Reagent bottles, Rotor Adapter holder, Rotor adapters, Sample tubes CB, Sample tubes RB and Spin column adapter rings?
FAQ ID -3054
View
What is the part number for O-rings for QIAsymphony SP and AS instruments? How often should the O-rings be replaced?
FAQ ID -3055
View
What is the thermal output for a BioRobot MDx and BioRobot Universal?
FAQ ID -3056
View
What is the thermal output for a QIAsymphony SP and AS instrument?
FAQ ID -3057
View
How precise is the QIAgility?
FAQ ID -3063
View
What are the advantages and disadvantages of turning on the tip “re-use” feature on the QIAgility?
FAQ ID -3064
View
How long does it take to set up a 96-well plate PCR reaction on the QIAgility?
FAQ ID -3066
View
Can I use a bar code scanner or reader on the QIAgility?
FAQ ID -3067
View
How can I find the run parameters for a BioSprint protocol that I am running?
FAQ ID -3069
View
Can BioSprint 96 be connected to LIMS?
FAQ ID -3070
View
What is the expected level of endotoxins in plasmid DNA purified with QIAprep spin miniprep kit?
FAQ ID -3081
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What is the expected level of endotoxins in plasmid DNA purified with QIAGEN Plasmid kits, QIAfilter Plasmid kits and HiSpeed Plasmid purification kits?
FAQ ID -3082
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What is the concentration of the Gentra RNase A that is used with the Autopure and Puregene protocols?
FAQ ID -3083
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What is the concentration of the different Proteinase K solutions sold by QIAGEN?
FAQ ID -3084
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Where can I access Material Safety Data Sheets (MSDS) online for detailed information on the reagents for each respective kit?
FAQ ID -3085
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What tubes can be used with TissueLyser Adapter Set 2 x 96 (cat. no. 69984)?
FAQ ID -3086
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Is there a need to compensate for additional liquid volume added with the positive control DNA, or a buccal swab lysate in the Investigator IDplex GO! Kit?
FAQ ID -3088
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What is the safest procedure to add FTA punches to the PCR reactions of the Investigator IDplex GO! Kit?
FAQ ID -3096
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How to upload a Flexiplate on QIAGEN Webpage?
FAQ ID -3103
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Can we do modification XXXXX for custom siRNA that is not on the web page?
FAQ ID -3107
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What is the stability of siRNA lyophilized and resuspended?
FAQ ID -3109
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Do you offer FlexiTube or FlexiPlate siRNAs for rat?
FAQ ID -3114
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Is it possible to extract RNA from frozen blood?
FAQ ID -3119
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Is it possible to extract RNA from RNAprotect-stabilized-blood?
FAQ ID -3120
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What's the pulsed-field gel electrophoresis (PFGE) conditions of the gel picture showing on your website? http://www.qiagen.com/Search/MagAttract-HMW-DNA-Kit#productdetails
-48
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Can Dithiothreitol (DTT) be used instead of BME in Buffer RLT Plus of RNeasy Plus Mini Kit?
FAQ ID -3125
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Why does my purified DNA/RNA sometimes have a 260/280 or 260/230 ratio of more than 2? Does that mean the purity is poor?
FAQ ID -3132
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How do I remove the RNA contamination from purified DNA?
FAQ ID -3133
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What is the final concentration of Protease upon reconstitution in the QIAamp DNA Blood Mini?
FAQ ID -3140
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What is the Spike-in-Control in the miRNeasy Serum/Plasma Kit ?
FAQ ID -3169
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What is the difference between the 1 nmol FlexiTube and FlexiTube Gene Solution siRNAs?
FAQ ID -3174
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What could be a reason for cloudy precipitate in PCR reaction with STR Plus chemistry after cycling protocol is completed?
FAQ ID -3175
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What is needed for performing delta delta Ct relative quantitation on the Rotor-Gene Q?
FAQ ID -3178
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Is REST software Windows 7 compatible?
FAQ ID -3182
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Can the Rotor-Gene Q perform gradient PCR?
FAQ ID -3183
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What is the expected amplicon size of the Microbial DNA qPCR Assays?
FAQ ID — 3396
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What is the difference between Positive PCR Control (PPC) and Microbial DNA Positive Control?
FAQ ID — 3397
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What is LLOQ?
FAQ ID — 3401
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What is the difference between LLOQ and LOD?
FAQ ID — 3402
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What species are detected by the Pan Bacteria 1 and Pan Bacteria 3 Assays?
FAQ ID - 3427
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What is the composition of Buffer AC in the QIAamp UltraSens Virus Kit?
FAQ ID - 3429
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Can the miScript II RT Kit be used to quantify plant miRNAs?
FAQ ID - 3431
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What is the dissociation curve temperature of PCR products generated with the miScript Plant qPCR system?
FAQ ID - 3444
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Can specific types of white blood cells be enriched from a PAXgene Blood RNA Tube prior to the RNA isolation?
FAQ ID - 3467
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What is the proper disposal procedure for waste from the sample preparation?
FAQ ID - 3470
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What is the expected yield from 8.5 ml of blood?
FAQ ID - 3496
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What does the resuspension buffer, Buffer BG4, in the PAXgene Blood DNA kit contain?
FAQ ID - 3501
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How should blood samples drawn into PAXgene Blood DNA Tubes be transported?
FAQ ID - 3507
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How long can samples in PAXgene Blood DNA Tubes be stored at room temperature?
FAQ ID - 3508
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What is the expected DNA yield from 2.5 ml of blood when using the QIAsymphony, QIAcube, and salting-out precipitation method?
FAQ ID - 3510
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Which downstream applications have been tested with DNA purified from samples collected in PAXgene Blood DNA Tubes IVD?
FAQ ID - 3512
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What does PAXgene Tissue STABILIZER contain?
FAQ ID - 3513
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What is the minimum number of cells that can be efficiently processed with the QIAamp DNA Mini and QIAamp Blood Mini kits and what is the expected yield?
FAQ ID - 3516
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Is the quality and size of DNA extracted with the DNeasy Blood & Tissue kit good enough to generate DNA-libraries for next generation sequencing?
FAQ ID - 3517
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What is the minimum number of cells or minimum amount of tissue that can be efficiently processed with the RNeasy mini kit?
FAQ ID - 3519
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Can I use the RNeasy Fibrous Tissue Mini Kit (cat. no. 74704) to extract RNA from fibrous plant material?
FAQ ID - 3521
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Will the Reverse transcription control on the RT2 profiler PCR array work on any cDNA library?
FAQ ID - 3534
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Microscopic/visual confirmation of successfully sorted cells can be used to optimize sorting conditions. Can stained cells be used?
FAQ ID - 3559
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Is the miRNA reverse transcription control (miRTC) used in conjunction with the miScript Single Cell qPCR Kit?
FAQ ID - 3573
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Is clot detection possible on the QIAsymphony?
FAQ - ID 3588
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How can I determine if a tube is suitable for clot detection on QIAsymphony?
FAQ ID - 3590
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How should I thaw frozen plasma samples?
FAQ ID - 3645
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How do Unique Molecular Indexes (UMIs) improve quantification?
FAQ ID - 3669
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Can REPLI-g WGA be used to amplify cDNA if the cDNA is dsDNA (double-stranded DNA)?
FAQ ID - 3686
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I would like to stop during the library preparation procedure. When can I do that?
FAQ ID - 3687
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Which regions are covered by the GR DNAseq V2 Panels (#181900)?
FAQ ID - 3688
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When preparing plasma with the PAXgene Blood ccfDNA tubes, cat. # 768115, do you recommend that the first centrifugation step should be done with the brakes on or off?
FAQ ID - 3690
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How do I insert and replace an absorber strip into the PyroMark Q48 Autoprep chamber?
FAQ ID - 3691
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Are peak heights of Q48 comparable to other PyroMark instruments?
FAQ ID - 3692
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How much space does the PyroMark Q48 Autoprep instrument need?
FAQ ID - 3693
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Can unused wells in a pyrosequencing discs be used in the next run?
FAQ ID - 3694
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Where can I find the certificate of calibration for the Rotor-Disc OTV Kit (catalog number 981400)?
FAQ ID - 3695
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What is the minimum guaranteed shelf-life of the PAXgene Blood ccfDNA Tubes (100) (catalog no. 768115)?
FAQ ID - 3696
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What methods are used during normalization of RNAseq data in the secondary GeneGlobe Data Analysis Center?
FAQ ID - 3698
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How do I prepare Buffer MP?
FAQ ID - 3620
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Are the QIAshredder columns included in various QIAGEN kits the same?
FAQ ID - 3624
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What is the composition of buffer QBT?
FAQ ID -411
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What is the composition of buffer QC?
FAQ ID -412
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What is the composition of buffer QF?
FAQ ID -413
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What is the composition of buffer STE?
FAQ ID -415
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