Product FAQ - QIAGEN



Product FAQ Bookmark Share more.. You are not authorized to download the resource Sort options Sort alphabetically Sort by relevance Sorted by relevance Why are the RT² qPCR Primer Assays not designed to cross exon-intron junctions or boundaries? FAQ ID -2710 View Why should I use RT² SYBR Green Mastermix with RT² qPCR Primer Assays? FAQ ID -2706 View Will pipetting error affect the EpiTect Methyl qPCR Array results? FAQ ID -2741 View Will pipetting error affect the miRNA qPCR Assay results? FAQ ID -2728 View Why had my RT² SYBR Green Mastermix been working well in the past, but now does not seem to be? FAQ ID -2717 View What MOI should I use for my cells? FAQ ID -2770 View What RT² qPCR Primer Assays are available? FAQ ID -2708 View What transfection reagents are compatible with the Cignal Reporter Assays? FAQ ID -2764 View What is the most reliable transfection reagent for delivering shRNA plasmids and siRNA to cells in culture? FAQ ID -2777 View What are the key parameters contributing to unwanted off-target effects in an RNAi experiment? FAQ ID -2775 View What are the structures of the siRNA molecules used in RNAi studies? FAQ ID -2760 View What are the critical factors in designing the siRNA molecules to be used for RNAi studies? FAQ ID -2759 View Why is my EpiTect Methyl qPCR Assay "failed" as indicated in the QC page of the data analysis Excel file? FAQ ID -2734 View Why is the qRT-PCR reproducibility so critical when detecting gene expression knock down in an RNAi experiment? FAQ ID -2774 View Can miScript miRNA qPCR Assays be used for pre-miRNA detection? FAQ ID -2724 View Can miScript miRNA qPCR Assays detect single nucleotide difference between miRNAs? FAQ ID -2723 View Do I need an internal methylated DNA reference in the EpiTect Methyl qPCR Array? FAQ ID -2736 View Do I need the sequence specific miRNA primers in the RT step of the miRNA qPCR Assays? FAQ ID -2725 View Do you always run samples in triplicates? FAQ ID -2703 View Can you use stained (H & E) sections with RT² PreAMP cDNA Synthesis Kit? FAQ ID -2722 View Does this method with the EpiTect Methyl qPCR Array detect methylation at specific CpG dinucleotides? FAQ ID -2737 View Does QIAGEN guarantee the performance of the SureSilencing shRNA Plasmids? FAQ ID -2787 View Can I make stable cell lines with the Cignal Reporter Assays? FAQ ID -2763 View How are the primers in the EpiTect Methyl qPCR Array designed? FAQ ID -2735 View Is it acceptable to correct for transfection efficiency, when determining the level of gene expression knock down by an shRNA expression vector? FAQ ID -2783 View Should I use monoclonal or polyclonal antibodies in EpiTect ChIP assays? FAQ ID -2747 View What are the advantages of using the SureSilencing shRNA Plasmids over siRNA? FAQ ID -2786 View The pathway reporter luciferase activity values are greater than the positive control, is there a problem? FAQ ID -2766 View The EpiTect ChIP qPCR primers I used show very high Ct. Are there any solutions? FAQ ID -2750 View The pathway reporter luciferase activity values are less than the negative control, is there a problem? FAQ ID -2767 View How can I prevent the evaporation of reaction volume from the wells EpiTect Methyl qPCR Arrays? FAQ ID -2742 View How can RNA interference be used as a life science research tool? FAQ ID -2756 View How can I be sure that the restriction enzyme digestion is complete in the EpiTect Methyl qPCR system? FAQ ID -2731 View How can I normalize my EpiTect ChIP results? FAQ ID -2751 View What is the recommended amount of input template for each RT² qPCR Primer Assay? FAQ ID -2716 View What is the RT² Profiler PCR Array? FAQ ID -2718 View Can the EpiTect Methyl qPCR System technology reliably characterize heterogenous tissue samples? FAQ ID -2732 View Can users insert custom transcriptional regulatory elements or promoters into the Cignal Reporter Assays? FAQ ID -2765 View Can you custom design the Epitect qPCR primers for the CpG island outside of the defined promoter region? FAQ ID -2744 View Can I manually set the threshold line? FAQ ID -2702 View Can I use your EpiTect One-Day kit with transcription factors? FAQ ID -2752 View Can I obtain the sequence of the RT² qPCR Primer Assay that I purchased? FAQ ID -2709 View Are EpiTect Methyl qPCR Arrays or Assays applicable to FFPE samples? FAQ ID -2733 View Are there any unique elements that need to be incorporated into an shRNA expression vector? FAQ ID -2772 View Can I use the default PCR program to run EpiTect Methyl qPCR Arrays or Assays? FAQ ID -2745 View Can I use total RNA for the miRNA PCR Arrays or Assays? FAQ ID -2726 View Can I reliably detect intermediately methylated DNA with EpiTect Methyl qPCR Array technology? FAQ ID -2738 View Can I use your EpiTect ChIP system in a RNA study? FAQ ID -2748 View Can I transform the Cignal Reporter Assays? FAQ ID -2762 View How many experiments can I do with the Cignal Lenti Reporter Assays? FAQ ID -2769 View Can I use fluorescence microscopy to assess shRNA plasmid transfection efficiency in my model cell line of interest, when using a vector expressing a GFP reporter gene? FAQ ID -2782 View How many housekeeping genes are included in each PCR Array? FAQ ID -2704 View How reliable are the results from the miScript Primer Assays? FAQ ID -2730 View What are EpiTect ChIP qPCR Assays? FAQ ID -2720 View What are the advantages of EpiTect ChIP qPCR Assays and Arrays? FAQ ID -2721 View How do I choose EpiTect ChIP-grade antibodies? FAQ ID -2746 View How do you determine the efficiency using the PCR array? FAQ ID -2701 View How does QIAGEN control the quality of the RT² qPCR Primer Assays? FAQ ID -2711 View How do I choose between the DNA-based Cignal Reporter Assays and the Cignal Lenti Reporter Assays? FAQ ID -2761 View Is the Microbial qPCR mastermix used in the Microbial DNA assay and in the Microbial DNA arrays free of genomic bacterial DNA? FAQ ID - 3535 View Show more results Back to top Contact QIAGEN Global contacts Technical Service Customer Care